Strain Name:

B6.129S1-Jag2tm1Grid/J

Stock Number:

010546

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Availability:

Cryopreserved - Ready for recovery

Homozygous Jag2deltaDSL mutant mice exhibit syndactyly and craniofacial defects that cause perinatal lethality. Heterozygotes have abnormal inner ear morphology. This mutant mouse strain may be useful in studies of craniofacial, limb and inner ear development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN10pN1
Generation Definitions
 
Donating Investigator Tom Gridley,   Maine Medical Center Research Institute

Description
Mice that are homozygous for this targeted mutation have a perinatal lethal phenotype, dying shortly after birth due to craniofacial defects (cleft palate, due to fusion of unelevated palatal shelves with the tongue). Embryos homozygous for the mutation and aged embryonic day 10.5-11.5, display a hyperplastic thick apical ectodermal ridge of the limb buds. Homozygous neonates have bilateral cleft of the secondary palate and syndactyly (fused digits) of both forelimbs and hindlimbs, although the hindlimbs are more affected. The number of inner ear hair cells is increased in mutants. Histological analysis reveals an increased number of inner ear hair cells and disorganized stereocilia bundles in mutants. Homozygotes exhibit abnormal thymic morphology and decreased gamma-delta T cell number. Heterozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Heterozygotes have abnormal inner ear morphology (increased number of hair cells).

Development
A targeting vector containing a PGK-neo cassette was used to disrupt 5.0kb of sequence containing the exons which encode the DSL domain and half of the first EGF repeat. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J females, and then C57BL/6J for 10 generations.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
007664   129S-Efnb1tm1Sor/J
000646   A/J
000647   A/WySnJ
005709   B6.129-Skitm1Cco/J
002619   B6.129-Tgfb3tm1Doe/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
002711   B6;129-Gabrb3tm1Geh/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010618   B6;129S-Jag1tm2Grid/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
022358   B6;129S6-Rr23tm1Axvi/Mmjax
022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
002990   B6;129S7-Inhbatm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCebOve/J
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
003318   STOCK Shhtm1Amc/J
003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
008469   STOCK Wnt9btm1.2Amc/J
View Facebase: models     (61 strains)

Strains carrying other alleles of Jag2
000239   STOCK Jag2sm/J
View Strains carrying other alleles of Jag2     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Jag2tm1Grid/Jag2+

        involves: 129S1/Sv * C57BL/6J
  • hearing/vestibular/ear phenotype
  • increased cochlear hair cell number
    • heterozygotes exhibit a slight but insignificant increase in average IHC density and total number relative to wild-type mice   (MGI Ref ID J:53432)
    • increased cochlear inner hair cell number
      • heterozygotes display a relatively high incidence of IHC duplications relative to wild-type mice   (MGI Ref ID J:53432)
  • nervous system phenotype
  • increased cochlear hair cell number
    • heterozygotes exhibit a slight but insignificant increase in average IHC density and total number relative to wild-type mice   (MGI Ref ID J:53432)
    • increased cochlear inner hair cell number
      • heterozygotes display a relatively high incidence of IHC duplications relative to wild-type mice   (MGI Ref ID J:53432)

Jag2tm1Grid/Jag2tm1Grid

        involves: 129S1/Sv * C57BL/6J
  • mortality/aging
  • complete perinatal lethality
    • all homozygotes die within a few minutes after birth because of craniofacial defects   (MGI Ref ID J:46930)
  • respiratory system phenotype
  • respiratory distress
    • most newborn homozygotes are unable to breathe   (MGI Ref ID J:46930)
    • only a few are able to breathe, but all of these die within a few hours after birth with large amounts of air in the stomach and intestines   (MGI Ref ID J:46930)
  • homeostasis/metabolism phenotype
  • cyanosis
    • newborn homozygotes become rapidly cyanotic   (MGI Ref ID J:46930)
  • craniofacial phenotype
  • absent maxillary shelf   (MGI Ref ID J:46930)
  • absent palatine shelf   (MGI Ref ID J:46930)
  • cleft secondary palate
    • at E18 (~1 day prior to birth), all homozygotes display a bilateral cleft of the secondary palate   (MGI Ref ID J:46930)
    • failure of palatal shelf elevation
      • palatal shelves fail to elevate and fuse in the dorsal midline   (MGI Ref ID J:46930)
    • palatal shelf fusion with tongue or mandible
      • at E18 (~1 day prior to birth), homozygotes display fusion of the tongue with the unelevated palatal shelves, preventing proper formation of the oral cavity   (MGI Ref ID J:46930)
  • embryogenesis phenotype
  • thick apical ectodermal ridge
    • at E10.5-E11.5, homozygotes display a hyperplastic AER   (MGI Ref ID J:46930)
    • however, no decreased AER programmed cell death is noted at E10.5   (MGI Ref ID J:46930)
  • limbs/digits/tail phenotype
  • abnormal foot plate morphology
    • at E13.5, mutant foot plates are rounder and do not exhibit the same degree of interdigital clefting as wild-type foot plates   (MGI Ref ID J:46930)
  • abnormal phalanx morphology
    • 5 of 13 newborn homozygotes show primary chondrogenic or secondary osseous fusions of the distal phalanges, with the hindfeet more severely affected   (MGI Ref ID J:46930)
    • several newborns exhibit splitting of the terminal phalanx of digit 2 of the hindfeet   (MGI Ref ID J:46930)
  • syndactyly
    • newborn homozygotes exhibit syndactyly of the fore- and hindlimbs   (MGI Ref ID J:46930)
    • syndactyly is associated with reduced interdigital cell death in digits 2, 3, and 4, and often involves soft tissue fusions   (MGI Ref ID J:46930)
    • hindlimbs are more severely affected than forelimbs   (MGI Ref ID J:46930)
  • thick apical ectodermal ridge
    • at E10.5-E11.5, homozygotes display a hyperplastic AER   (MGI Ref ID J:46930)
    • however, no decreased AER programmed cell death is noted at E10.5   (MGI Ref ID J:46930)
  • digestive/alimentary phenotype
  • absent maxillary shelf   (MGI Ref ID J:46930)
  • absent palatine shelf   (MGI Ref ID J:46930)
  • cleft secondary palate
    • at E18 (~1 day prior to birth), all homozygotes display a bilateral cleft of the secondary palate   (MGI Ref ID J:46930)
    • failure of palatal shelf elevation
      • palatal shelves fail to elevate and fuse in the dorsal midline   (MGI Ref ID J:46930)
    • palatal shelf fusion with tongue or mandible
      • at E18 (~1 day prior to birth), homozygotes display fusion of the tongue with the unelevated palatal shelves, preventing proper formation of the oral cavity   (MGI Ref ID J:46930)
  • immune system phenotype
  • abnormal gamma-delta T cell differentiation
    • at E18, homozygotes display impaired differentiation of gamma-delta T lymphocyte lineage   (MGI Ref ID J:46930)
    • in contrast, differentiation of alpha-beta T cells is unaffected   (MGI Ref ID J:46930)
  • abnormal thymus morphology
    • at E18 (~1 day prior to birth), homozygotes display altered thymic morphology   (MGI Ref ID J:46930)
    • abnormal thymus medulla morphology
      • at E18, both the number and the size of developing medullary regions are reduced   (MGI Ref ID J:46930)
  • decreased gamma-delta T cell number
    • the total percentage of gamma-delta T cells, as well as those among the CD4-/CD8- double negative subset, is about one-half that observed in wild-type thymi   (MGI Ref ID J:46930)
  • hearing/vestibular/ear phenotype
  • abnormal cochlear outer hair cell morphology
    • at E18, patterning in OHC rows is much more irregular than in wild-type   (MGI Ref ID J:62727)
    • increased cochlear outer hair cell number   (MGI Ref ID J:62727)
      • at E18, homozygotes contain four rather than three rows of OHCs in some regions of the sensory epithelium   (MGI Ref ID J:53432)
  • abnormal orientation of inner hair cell stereociliary bundles
    • at E18, homozygotes often exhibit abnormal orientation of IHC stereociliary bundles   (MGI Ref ID J:53432)
  • decreased organ of Corti supporting cell number
    • at E18, the overall pattern of non-sensory supporting cells is present; however, some regions appear to contain a reduced number of supporting cell nuclei   (MGI Ref ID J:53432)
    • some pairs of IHCs appear to be in contact with one another, suggesting absence of interdigitating supporting cells at these positions   (MGI Ref ID J:53432)
  • increased cochlear hair cell number   (MGI Ref ID J:62727)
    • at E18, homozygotes exhibit a significant increase in the total number and density of cochlear hair cells, primariliy due to an increase in IHCs   (MGI Ref ID J:53432)
    • a significant reduction in the ratio of OHCs to IHCs is observed   (MGI Ref ID J:53432)
    • increased cochlear inner hair cell number   (MGI Ref ID J:62727)
      • at E18, homozygotes display multiple IHC duplications, resulting in the addition of a nearly complete second row of IHCs   (MGI Ref ID J:53432)
    • increased cochlear outer hair cell number   (MGI Ref ID J:62727)
      • at E18, homozygotes contain four rather than three rows of OHCs in some regions of the sensory epithelium   (MGI Ref ID J:53432)
  • nervous system phenotype
  • abnormal cochlear outer hair cell morphology
    • at E18, patterning in OHC rows is much more irregular than in wild-type   (MGI Ref ID J:62727)
    • increased cochlear outer hair cell number   (MGI Ref ID J:62727)
      • at E18, homozygotes contain four rather than three rows of OHCs in some regions of the sensory epithelium   (MGI Ref ID J:53432)
  • abnormal orientation of inner hair cell stereociliary bundles
    • at E18, homozygotes often exhibit abnormal orientation of IHC stereociliary bundles   (MGI Ref ID J:53432)
  • increased cochlear hair cell number   (MGI Ref ID J:62727)
    • at E18, homozygotes exhibit a significant increase in the total number and density of cochlear hair cells, primariliy due to an increase in IHCs   (MGI Ref ID J:53432)
    • a significant reduction in the ratio of OHCs to IHCs is observed   (MGI Ref ID J:53432)
    • increased cochlear inner hair cell number   (MGI Ref ID J:62727)
      • at E18, homozygotes display multiple IHC duplications, resulting in the addition of a nearly complete second row of IHCs   (MGI Ref ID J:53432)
    • increased cochlear outer hair cell number   (MGI Ref ID J:62727)
      • at E18, homozygotes contain four rather than three rows of OHCs in some regions of the sensory epithelium   (MGI Ref ID J:53432)
  • hematopoietic system phenotype
  • abnormal gamma-delta T cell differentiation
    • at E18, homozygotes display impaired differentiation of gamma-delta T lymphocyte lineage   (MGI Ref ID J:46930)
    • in contrast, differentiation of alpha-beta T cells is unaffected   (MGI Ref ID J:46930)
  • abnormal thymus morphology
    • at E18 (~1 day prior to birth), homozygotes display altered thymic morphology   (MGI Ref ID J:46930)
    • abnormal thymus medulla morphology
      • at E18, both the number and the size of developing medullary regions are reduced   (MGI Ref ID J:46930)
  • decreased gamma-delta T cell number
    • the total percentage of gamma-delta T cells, as well as those among the CD4-/CD8- double negative subset, is about one-half that observed in wild-type thymi   (MGI Ref ID J:46930)
  • skeleton phenotype
  • abnormal phalanx morphology
    • 5 of 13 newborn homozygotes show primary chondrogenic or secondary osseous fusions of the distal phalanges, with the hindfeet more severely affected   (MGI Ref ID J:46930)
    • several newborns exhibit splitting of the terminal phalanx of digit 2 of the hindfeet   (MGI Ref ID J:46930)
  • absent maxillary shelf   (MGI Ref ID J:46930)
  • absent palatine shelf   (MGI Ref ID J:46930)

Jag2tm1Grid/Jag2tm1Grid

        involves: 129S1/Sv * 129S1/SvImJ * C57BL/6J
  • mortality/aging
  • complete postnatal lethality
    • mice do not survive postnatally   (MGI Ref ID J:132241)
  • hearing/vestibular/ear phenotype
  • abnormal orientation of cochlear hair cell stereociliary bundles
    • hair cell stereocilia bundles exhibit some disorganization   (MGI Ref ID J:132241)
  • increased cochlear inner hair cell number   (MGI Ref ID J:132241)
  • increased cochlear outer hair cell number   (MGI Ref ID J:132241)
  • nervous system phenotype
  • abnormal orientation of cochlear hair cell stereociliary bundles
    • hair cell stereocilia bundles exhibit some disorganization   (MGI Ref ID J:132241)
  • increased cochlear inner hair cell number   (MGI Ref ID J:132241)
  • increased cochlear outer hair cell number   (MGI Ref ID J:132241)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Craniofacial and Palate Defects
      congenital cleft palate
Skeletal Defects
      syndactyly

Neurobiology Research
Hearing Defects

Research Tools
Immunology, Inflammation and Autoimmunity Research
      specific T cell deficiency

Sensorineural Research
Hearing Defects

Jag2tm1Grid related

Developmental Biology Research
Craniofacial and Palate Defects
      congenital cleft palate
Embryonic Lethality (Homozygous)
Postnatal Lethality
      Homozygous
Skeletal Defects
      syndactyly

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      specific T cell deficiency

Internal/Organ Research
Thymus Defects

Sensorineural Research
Hearing Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Jag2tm1Grid
Allele Name targeted mutation 1, Tom Gridley
Allele Type Targeted (knock-out)
Common Name(s) Jag2-; Jag2deltaDSL;
Mutation Made By Tom Gridley,   Maine Medical Center Research Institute
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameCJ7
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Jag2, jagged 2
Chromosome 12
Gene Common Name(s) D12Ggc2e; DNA segment, Chr 12, G. Consalez 2, expressed; HJ2; SER2; Serh; serrate homolog (Drosophila); sm; syndactylism;
Molecular Note The gene was disrupted by replacement of a 5 kb region with a PGK-neo cassette via recombination resulting in deletion of the DSL domain and half of the first EGF repeat. The absence of the DSL domain-containing sequence was confirmed by Southern blot analysis. [MGI Ref ID J:46930]

Genotyping

Genotyping Information

Genotyping Protocols

Jag2tm1Grid, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jiang R; Lan Y; Chapman HD; Shawber C; Norton CR; Serreze DV; Weinmaster G; Gridley T. 1998. Defects in limb, craniofacial, and thymic development in Jagged2 mutant mice. Genes Dev 12(7):1046-57. [PubMed: 9531541]  [MGI Ref ID J:46930]

Additional References

Jag2tm1Grid related

Alappat SR; Zhang Z; Suzuki K; Zhang X; Liu H; Jiang R; Yamada G; Chen Y. 2005. The cellular and molecular etiology of the cleft secondary palate in Fgf10 mutant mice. Dev Biol 277(1):102-13. [PubMed: 15572143]  [MGI Ref ID J:95764]

Blackburn J; Ohazama A; Kawasaki K; Otsuka-Tanaka Y; Liu B; Honda K; Rountree RB; Hu Y; Kawasaki M; Birchmeier W; Schmidt-Ullrich R; Kinoshita A; Schutte BC; Hammond NL; Dixon MJ; Sharpe PT. 2012. The role of Irf6 in tooth epithelial invagination. Dev Biol 365(1):61-70. [PubMed: 22366192]  [MGI Ref ID J:184926]

Casey LM; Lan Y; Cho ES; Maltby KM; Gridley T; Jiang R. 2006. Jag2-Notch1 signaling regulates oral epithelial differentiation and palate development. Dev Dyn 235(7):1830-1844. [PubMed: 16607638]  [MGI Ref ID J:108824]

Jin JZ; Tan M; Warner DR; Darling DS; Higashi Y; Gridley T; Ding J. 2010. Mesenchymal cell remodeling during mouse secondary palate reorientation. Dev Dyn 239(7):2110-7. [PubMed: 20549719]  [MGI Ref ID J:161213]

Kiernan AE; Cordes R; Kopan R; Gossler A; Gridley T. 2005. The Notch ligands DLL1 and JAG2 act synergistically to regulate hair cell development in the mammalian inner ear. Development 132(19):4353-62. [PubMed: 16141228]  [MGI Ref ID J:132241]

Lanford PJ; Lan Y; Jiang R; Lindsell C; Weinmaster G; Gridley T ; Kelley MW. 1999. Notch signalling pathway mediates hair cell development in mammalian cochlea [see comments] Nat Genet 21(3):289-92. [PubMed: 10080181]  [MGI Ref ID J:53432]

Mitsiadis TA; Graf D; Luder H; Gridley T; Bluteau G. 2010. BMPs and FGFs target Notch signalling via jagged 2 to regulate tooth morphogenesis and cytodifferentiation. Development 137(18):3025-35. [PubMed: 20685737]  [MGI Ref ID J:164590]

Mitsiadis TA; Regaudiat L; Gridley T. 2005. Role of the Notch signalling pathway in tooth morphogenesis. Arch Oral Biol 50(2):137-40. [PubMed: 15721140]  [MGI Ref ID J:98508]

Richardson RJ; Dixon J; Jiang R; Dixon MJ. 2009. Integration of IRF6 and Jagged2 signalling is essential for controlling palatal adhesion and fusion competence. Hum Mol Genet 18(14):2632-42. [PubMed: 19439425]  [MGI Ref ID J:150009]

Robert-Moreno A; Guiu J; Ruiz-Herguido C; Lopez ME; Ingles-Esteve J; Riera L; Tipping A; Enver T; Dzierzak E; Gridley T; Espinosa L; Bigas A. 2008. Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1. EMBO J 27(13):1886-95. [PubMed: 18528438]  [MGI Ref ID J:137647]

Worsley AG; Leibundgut-Landmann S; Slack E; Phng LK; Gerhardt H; Sousa CR; Macdonald AS. 2008. Dendritic cell expression of the Notch ligand jagged2 is not essential for Th2 response induction in vivo. Eur J Immunol 38(4):1043-9. [PubMed: 18350543]  [MGI Ref ID J:133948]

Yao Y; Yao J; Radparvar M; Blazquez-Medela AM; Guihard PJ; Jumabay M; Bostrom KI. 2013. Reducing Jagged 1 and 2 levels prevents cerebral arteriovenous malformations in matrix Gla protein deficiency. Proc Natl Acad Sci U S A 110(47):19071-6. [PubMed: 24191040]  [MGI Ref ID J:202978]

Zhang N; Martin GV; Kelley MW; Gridley T. 2000. A mutation in the Lunatic fringe gene suppresses the effects of a Jagged2 mutation on inner hair cell development in the cochlea. Curr Biol 10(11):659-62. [PubMed: 10837254]  [MGI Ref ID J:62727]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have a perinatal lethal phenotype.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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