Strain Name:

B6;SJL-Tg(Prl-tTA)6-5Jek/J

Stock Number:

010573

Availability:

Under Development for Distribution Colony

Use Restrictions Apply, see Terms of Use
To register your interest in this strain go to the Strain Interest Form.
These Prl-tTA transgenic mice have expression of the tetracycline-controlled transactivator (tTA) protein directed to PRL-producing cells (pituitary lactotropes/mammotropes) by the rat prolactin (Prl) promoter. The donating investigator also reports some expression in testis. These mice are a Tet-Off tool that allows dox-conditional expression of genes in PRL-producing cells/tissues and may be useful for studying pituitary hormone secretion and proliferation.

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Jeffrey Kudlow,   University of Alabama at Birmingham

Description
The donating investigator claims homozygous mice are viable and fertile. These Prl-tTA transgenic mice have expression of the tetracycline-controlled transactivator (tTA) protein directed to PRL-producing cells (pituitary lactotropes/mammotropes) by the rat prolactin (Prl) promoter. The donating investigator also reports some expression in testis. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene in the PRL-producing cells may be conditionally abolished with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These Prl-tTA transgenic mice are a Tet-Off tool that allows dox-conditional expression of genes in PRL-producing cells/tissues (such as pituitary lactotropes/mammotropes) and may be useful for studying pituitary hormone secretion and proliferation.

Development
The PRL-tTA transgene was designed with a 3.2 kb promoter sequence from the rat prolactin (Prl) gene, a tetracycline-regulated transactivator (tTA) gene, and an SV40 intron/polyA sequence. This 5.2 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 6-5 were bred with C57BL/6;SJL wild-type mice to establish the colony. The donating investigator reports that PRL-tTA transgenic mice were bred together or to tetO-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the PRL-tTA transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.

Control Information

  Control
   Noncarrier
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Prl
006101   B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J
View Strains carrying other alleles of Prl     (1 strain)

View Strains carrying other alleles of tTA     (22 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Endocrine Deficiency Research
Hypothalamus/Pituitary Defects
      control strain

Neurobiology Research
Tet Expression System
      tTA/rtTA Expressing Strains

Research Tools
Cancer Research
      Tetop Tet System
Cardiovascular Research
      Tetop Tet System
Endocrine Deficiency Research
Genetics Research
      Mutagenesis and Transgenesis: Tetop Tet System
      Tissue/Cell Markers
Neurobiology Research
      Tetop Tet System
Reproductive Biology Research
      Tetop Tet System
      testicular cell marker
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Prl-tTA)6-5Jek
Allele Name transgene insertion 6-5, Jeffrey E Kudlow
Allele Type Transgenic (random, expressed)
Common Name(s) Prl-tTA;
Mutation Made By Jeffrey Kudlow,   University of Alabama at Birmingham
Strain of Origin(C57BL/6 x SJL)
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Prl, prolactin, rat
Molecular Note The PRL-tTA transgene was designed with a 3.2 kb promoter sequence from the rat prolactin (Prl) gene, a tetracycline-regulated transactivator (tTA) gene, and an SV40 intron/polyA sequence. This 5.2 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 6-5 were bred with C57BL/6;SJL wildtype mice to establish the colony. [MGI Ref ID J:68692]
 
 

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Roh M; Paterson AJ; Asa SL; Chin E; Kudlow JE. 2001. Stage-sensitive blockade of pituitary somatomammotrope development by targeted expression of a dominant negative epidermal growth factor receptor in transgenic mice. Mol Endocrinol 15(4):600-13. [PubMed: 11266511]  [MGI Ref ID J:68692]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development and On Hold

Supply Details

Standard SupplyUnder Development for Distribution Colony
Supply Notes

Control Information

  Control
   Noncarrier
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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