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| These TetRE-EGFR-tr (TRE-EGFR-tr) transgenic mice express a dominant negative, cytoplasmic tyrosine kinase domain-deleted mutant form of epidermal growth factor receptor (EGFR-tr) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. EGFR-tr expression disrupts subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation, and survival. These TetRE-EGFR-tr mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of a dominant negative EGF receptor, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins). | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Jeffrey Kudlow, University of Alabama at Birmingham Description
The donating investigator reports that homozygous mice are viable and fertile. These TetRE-EGFR-tr (TRE-EGFR-tr) transgenic mice express a dominant negative, cytoplasmic tyrosine kinase domain-deleted mutant form of epidermal growth factor receptor (EGFR-tr) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue EGFR-tr expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. EGFR-tr expression disrupts subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation, and survival. These TetRE-EGFR-tr mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of a dominant negative EGF receptor, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).For example, when bred to a strain expressing rtTA in pituitary somatotropes (see Stock No. 010574), this mutant mouse strain may be useful in studies of pituitary development.
When bred to a strain expressing tTA in pituitary lactotropes/mammotropes (see Stock No. 010573 for example), this mutant mouse strain may be useful in studies of pituitary development.
Development
The TetRE-EGFRtr-SV40 transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 2.3 kb C-terminal truncated mouse epidermal growth factor receptor cDNA sequence (EGFR-tr; encoding amino acid 1-690 followed by a TGA stop codon), and an SV40 intron/polyA sequence. This 4.5 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 2-9 were bred with C57BL/6;SJL wildtype mice to establish the colony. The donating investigator reports that TetRE-EGFR-tr transgenic mice were bred with rtTA-transgenic mice (on the same mixed C57BL/6;SJL genetic background), and then together or to wildtype siblings for many generations. Mice harboring only the TetRE-EGFR-tr transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Egfr
000553 B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J 006926 C57BL/6J-EgfrVel/J 002857 STOCK Egfrtm1Mag/J 000317 STOCK a/a Egfrwa2/J View Strains carrying other alleles of Egfr (4 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(Gh1-rtTA)4-3Jek/0 Tg(tetO-Egfr*)2-9Jek/0
involves: C57BL/6 * SJL
- mortality/aging
- postnatal lethality
- offspring of treated female mice die shortly after birth (MGI Ref ID J:68692)
- growth/size phenotype
- decreased body size
- by six weeks of age, animals display a dwarf phenotype; growth stops around 6 weeks (MGI Ref ID J:68692)
- slow postnatal weight gain
- at birth, weight of animals treated with doxycycline is comparable to controls but lags behind during postnatal period; when growth stops, mice have weights about half to two thirds that of control (MGI Ref ID J:68692)
- postnatal growth retardation
- pups from transgenic females treated with doxycycline during gestation are not distinguishable from controls animals during the initial 10 days post-natal; by 14 days, animals are visibly smaller than controls (MGI Ref ID J:68692)
- slow postnatal weight gain
- at birth, weight of animals treated with doxycycline is comparable to controls but lags behind during postnatal period; when growth stops, mice have weights about half to two thirds that of control (MGI Ref ID J:68692)
- endocrine/exocrine gland phenotype
- abnormal adenohypophysis morphology
- anterior lobe in doxycycline-treated mice (treatment from conception or from E14) are significantly smaller than wild-type at 6 weeks; posterior and intermediate lobes are normal (MGI Ref ID J:68692)
- abnormal gonadotroph morphology
- at 6 weeks, gonadotropin staining is slightly reduced compared to wild-type adults or age-matched controls (MGI Ref ID J:68692)
- decreased lactotroph cell number
- prolactin-staining cells show marked reduction in number (MGI Ref ID J:68692)
- decreased somatotroph cell number
- nearly complete absence of growth hormone-staining cells is observed at 6 weeks (MGI Ref ID J:68692)
- reproductive system phenotype
- abnormal reproductive system physiology
- dwarf females can carry pregnancies to birth, but offspring die shortly after birth, suggesting that the dams have impaired lactation (MGI Ref ID J:68692)
- nervous system phenotype
- abnormal adenohypophysis morphology
- anterior lobe in doxycycline-treated mice (treatment from conception or from E14) are significantly smaller than wild-type at 6 weeks; posterior and intermediate lobes are normal (MGI Ref ID J:68692)
- abnormal gonadotroph morphology
- at 6 weeks, gonadotropin staining is slightly reduced compared to wild-type adults or age-matched controls (MGI Ref ID J:68692)
- decreased lactotroph cell number
- prolactin-staining cells show marked reduction in number (MGI Ref ID J:68692)
- decreased somatotroph cell number
- nearly complete absence of growth hormone-staining cells is observed at 6 weeks (MGI Ref ID J:68692)
- vision/eye phenotype
- delayed eyelid opening
- eye opening of mutants is delayed by 2-3 days relative to wild-type or single positive controls (MGI Ref ID J:68692)
Tg(Prl-tTA)6-5Jek/0 Tg(tetO-Egfr*)2-9Jek/0
involves: C57BL/6 * SJL
- growth/size phenotype
- *normal* growth/size phenotype
- rates of growth and weight gain in transgenic mice after doxycycline-withdrawalare identical to wild-type mice (MGI Ref ID J:68692)
- endocrine/exocrine gland phenotype
- *normal* endocrine/exocrine gland phenotype
- pituitary size and pituitary gross morphology in transgenic mice after doxycycline withdrawal are the same as wild-type mice; cell proliferation in the pituitary shows no difference compared to controls (MGI Ref ID J:68692)
- reproductive system phenotype
- *normal* reproductive system phenotype
- ability of transgenic mice to lactate is not impaired (MGI Ref ID J:68692)
- homeostasis/metabolism phenotype
- *normal* homeostasis/metabolism phenotype
- pituitary hormones in transgenic mice after doxycycline-withdrawal show normal distribution patterns compared to wild-type mice; prolactin (PRL) shows a normal distribution pattern before and during pregnancy (MGI Ref ID J:68692)
This mouse can be used to support research in many areas including:
Cancer Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Cell Biology Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation
Developmental Biology Research
Defects in Cell Adhesion Molecules
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Neurobiology Research
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Cancer Research
Tetop Tet System
Cell Biology Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Mutagenesis and Transgenesis: transcriptional activation
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-Egfr*)2-9Jek | ||
|---|---|---|---|
| Allele Name | transgene insertion 2-9, Jeffrey E Kudlow | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TRE-EGFR-tr; TetRE-EGFR-tr; | ||
| Mutation Made By | Jeffrey Kudlow, University of Alabama at Birmingham | ||
| Strain of Origin | (C57BL/6 x SJL) | ||
| Expressed Gene | Egfr, epidermal growth factor receptor, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The TetRE-EGFRtr-SV40 transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 2.3 kb C-terminal truncated mouse epidermal growth factorreceptor cDNA sequence (EGFR-tr; encoding amino acid 1-690 followed by a TGA stop codon), and an SV40 intron/polyA sequence. This 4.5 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 2-9 were bred with C57BL/6;SJL wildtype mice to establish the colony. [MGI Ref ID J:68692] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Egfr*)2-9Jek STD PCR, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Roh M; Paterson AJ; Asa SL; Chin E; Kudlow JE. 2001. Stage-sensitive blockade of pituitary somatomammotrope development by targeted expression of a dominant negative epidermal growth factor receptor in transgenic mice. Mol Endocrinol 15(4):600-13. [PubMed: 11266511] [MGI Ref ID J:68692]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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