Strain Name:

B6;SJL-Tg(MMTV-rtTA)4-1Jek/J

Stock Number:

010576

Availability:

Under Development for Distribution Colony

Use Restrictions Apply, see Terms of Use
To register your interest in this strain go to the Strain Interest Form.
These MMTV-rtTA mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in males) as well as prostate glands. These mice are a Tet-On tool that allows conditional, dox-inducible expression of genes primarily in mammary gland epithelial cells and may be useful in studying the endocrine function of mammary tissues and/or breast cancer (for example).

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Jeffrey Kudlow,   University of Alabama at Birmingham

Description
The donating investigator claims homozygous mice are viable and fertile. These MMTV-rtTA mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in the males) as well as prostate glands. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene may be induced with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These MMTV-rtTA mice are a Tet-On tool that allows conditional, dox-inducible expression of genes primarily in mammary gland epithelial cells and may be useful in studying the endocrine function of mammary tissues and/or breast cancer (for example).

Development
The MMTV-rtTA-SV40 transgene was designed with the 1.5 kb mouse mammary tumor virus long terminal repeat (MMTV-LTR) promoter, the reverse tetracycline transactivator gene and VP16 activation domain (rtTA-VP16), and SV40 intron/polyA sequence. The rtTA-VP16-polyA sequence is inserted into exon 3 of the rabbit beta-globin gene immediately following the MMTV promoter. This 3.75 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 4-1 were bred with C57BL/6;SJL wildtype mice to establish the colony. The donating investigator reports that MMTV-rtTA transgenic mice were bred together, to wildtype siblings, and/or to tetO-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the MMTV-rtTA transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.

Control Information

  Control
   Noncarrier
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of rtTA     (22 strains)

View Strains carrying other alleles of MMTV     (19 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(MMTV-rtTA)4-1Jek/0

        involves: C57BL/6 * SJL
  • no phenotypic analysis
  • *normal* no phenotypic analysis (MGI Ref ID J:134284)
    • mice are viable; no phenotypic details provided
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Endocrine Deficiency Research
Mammary Gland Defects

Neurobiology Research
Tet Expression System
      tTA/rtTA Expressing Strains

Research Tools
Cancer Research
      Tetop Tet System
Endocrine Deficiency Research
Genetics Research
      Mutagenesis and Transgenesis: Tetop Tet System
      Tissue/Cell Markers
Neurobiology Research
      Tetop Tet System
Reproductive Biology Research
      Tetop Tet System
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(MMTV-rtTA)4-1Jek
Allele Name transgene insertion 4-1, Jeffrey E Kudlow
Allele Type Transgenic (random, expressed)
Common Name(s) MMTV-rtTA; MMTV-rtTA-SV40;
Mutation Made By Jeffrey Kudlow,   University of Alabama at Birmingham
Strain of Origin(C57BL/6 x SJL)
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
Molecular Note The MMTV-rtTA-SV40 transgene was designed with the 1.5 kb mouse mammary tumor virus long terminal repeat (MMTV-LTR) promoter, the reverse tetracycline transactivator gene and VP16 activation domain (rtTA-VP16), and SV40 intron/polyA sequence. The rtTA-VP16-polyA sequence is inserted into exon 3 of the rabbit beta-globin gene immediately following the MMTV promoter. This 3.75 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 4-1 were bred with C57BL/6;SJL wildtype mice to establish the colony. [MGI Ref ID J:134284]
 
 

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Whisenhunt TR; Yang X; Bowe DB; Paterson AJ; Van Tine BA; Kudlow JE. 2006. Disrupting the enzyme complex regulating O-GlcNAcylation blocks signaling and development. Glycobiology 16(6):551-63. [PubMed: 16505006]  [MGI Ref ID J:134284]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development and On Hold

Supply Details

Standard SupplyUnder Development for Distribution Colony
Supply Notes

Control Information

  Control
   Noncarrier
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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