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| These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice express an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed. These TetRE-ErbB2 mice may be useful in generating bi-transgenic mutant mice for the conditional (inducible/reversible) expression of a constitutively active ErbB2 isoform, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins). | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x Noncarrier (Female x Male) 23-MAR-10 Species laboratory mouse Generation ?+N1F3 (24-FEB-11)
Generation DefinitionsDonating Investigator Jeffrey Kudlow, University of Alabama at Birmingham Description
The donating investigator reports that homozygous mice are viable and fertile. These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice have expression of an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of the constitutively active ErbB2 protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed. These TetRE-ErbB2 mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of an activated ErbB2 isoform, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).Development
The TetRE-ErbB2 (pTRE-Neu-SV40) transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 4.8 kb cDNA sequence from the activated form of rat c-neu/erbB2 (ErbB2; with Val to Glu mutation within the transmembrane domain at amino acid 664), and an SV40 intron/polyA sequence. This 6.8 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 8-4 were bred with wildtype sibling mice to establish the colony. The donating investigator reports that TetRE-ErbB2 transgenic mice were bred together, and/or to tTA-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the TetRE-ErbB2 transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Erbb2
007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J View Strains carrying other alleles of Erbb2 (3 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cancer Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
Adenomas
Adenomas: lung, induced
Adenomas: multiple tissues - induced
Other Tissues/Organs
Other Tissues/Organs: lung, induced
Skin Cancers
Skin Cancers: Induced
Cell Biology Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation
Developmental Biology Research
Defects in Cell Adhesion Molecules
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Neurobiology Research
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Cancer Research
Tetop Tet System
Cell Biology Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Mutagenesis and Transgenesis: transcriptional activation
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-Erbb2*)8-4Jek | ||
|---|---|---|---|
| Allele Name | transgene insertion 8-4, Jeffrey E Kudlow | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TRE-ErbB2; TRE-Neu; TetRE-ErbB2; | ||
| Strain of Origin | (C57BL/6 x SJL) | ||
| Expressed Gene | Erbb2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), rat | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The TetRE-ErbB2 (pTRE-Neu-SV40) transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 4.8 kb cDNA sequence from the activated form ofrat c-neu/erbB2 (ErbB2; with Val to Glu mutation within the transmembrane domain at amino acid 664), and an SV40 intron/polyA sequence. This 6.8 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 8-4 were bred with wildtype sibling mice to establish the colony. [MGI Ref ID J:55970] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Erbb2*), Separated PCR
Tg(tetO-Erbb2*), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Xie W; Chow LT; Paterson AJ; Chin E; Kudlow JE. 1999. Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene 18(24):3593-607. [PubMed: 10380881] [MGI Ref ID J:55970]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control. Mating System Hemizygote x Noncarrier (Female x Male) 23-MAR-10 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(tetO-Erbb2*)8-4Jek
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(tetO-Erbb2*)8-4Jek x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(tetO-Erbb2*)8-4Jek Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(tetO-Erbb2*)8-4Jek
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(tetO-Erbb2*)8-4Jek x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(tetO-Erbb2*)8-4Jek Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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