| |||||||||||||||||||
| These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice express an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed. These TetRE-ErbB2 mice may be useful in generating bi-transgenic mutant mice for the conditional (inducible/reversible) expression of a constitutively active ErbB2 isoform, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins). | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Jeffrey Kudlow, University of Alabama at Birmingham Description
The donating investigator reports that homozygous mice are viable and fertile. These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice have expression of an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of the constitutively active ErbB2 protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed. These TetRE-ErbB2 mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of an activated ErbB2 isoform, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).Development
The TetRE-ErbB2 (pTRE-Neu-SV40) transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 4.8 kb cDNA sequence from the activated form of rat c-neu/erbB2 (ErbB2; with Val to Glu mutation within the transmembrane domain at amino acid 664), and an SV40 intron/polyA sequence. This 6.8 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 8-4 were bred with wildtype sibling mice to establish the colony. The donating investigator reports that TetRE-ErbB2 transgenic mice were bred together, and/or to tTA-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the TetRE-ErbB2 transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Erbb2
005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J View Strains carrying other alleles of Erbb2 (2 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cancer Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
Adenoma: multiple tissues - induced
Adenomas: lung, induced
Other Tissues/Organs: lung, induced
Skin Cancers: Induced
Cell Biology Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation
Developmental Biology Research
Defects in Cell Adhesion Molecules
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Neurobiology Research
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Cancer Research
Tetop Tet System
Cell Biology Research
Genetics Research
Mutagenesis and Transgenesis: Tetop Tet System
Mutagenesis and Transgenesis: transcriptional activation
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-Erbb2*)8-4Jek | ||
|---|---|---|---|
| Allele Name | transgene insertion 8-4, Jeffrey E Kudlow | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TRE-ErbB2; TRE-Neu; TetRE-ErbB2; | ||
| Strain of Origin | (C57BL/6 x SJL) | ||
| Expressed Gene | Erbb2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), rat | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The TetRE-ErbB2 (pTRE-Neu-SV40) transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 4.8 kb cDNA sequence from the activated form ofrat c-neu/erbB2 (ErbB2; with Val to Glu mutation within the transmembrane domain at amino acid 664), and an SV40 intron/polyA sequence. This 6.8 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 8-4 were bred with wildtype sibling mice to establish the colony. [MGI Ref ID J:55970] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Xie W; Chow LT; Paterson AJ; Chin E; Kudlow JE. 1999. Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene 18(24):3593-607. [PubMed: 10380881] [MGI Ref ID J:55970]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.