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| The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ser217/221Glu mutant mitogen-activated protein kinase kinase 1 (Map2k1). These mice develop a mild cardiac hypertrophy. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Jeffery D. Molkentin, Children's Hospital Medical Center Description
The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ser217/221Glu mutant mitogen-activated protein kinase kinase 1 (Map2k1). These mice develop a mild concentric cardiac ventricular hypertrophy. No premature lethality or cardiac dilation is observed in hemizygotes.Development
The mouse cardiac alpha myosin heavy chain (Myh6) promoter drives expression of an activated Ser217Glu/Ser221Glu form of mouse MEK1 (Map2k1). The strain was created and maintained on an FVB background by the donating laboratory. This is the "high-expressing" line; copy number was not determined.
Related Strains
Strains carrying other alleles of Myh6
View Strains carrying other alleles of Myh6 (38 strains)
Additional Web Information
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Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Cardiofaciocutaneous Syndrome (MAP2K1)
assigned by genotype
Tg(Myh6-Map2k1*)1Jmol/?
involves: FVB
- cardiovascular system phenotype
- decreased cardiomyocyte apoptosis
- less apoptosis is observed in mice subjected to ischemia/reperfusion of the left coronary artery (MGI Ref ID J:66102)
- decreased left ventricle diastolic pressure
- diastolic function is reduced due to decreased ventricular volume (MGI Ref ID J:66102)
- enlarged myocardial fiber
- about 30% increase in the cross-sectional area of each myocardial fiber (MGI Ref ID J:66102)
- heart left ventricle hypertrophy
- there is about a 50% increase left ventricular posterior wall thickness (MGI Ref ID J:66102)
- increased heart weight
- increased heart:body weight ratio of 34, 41, and 23% is observed at 3 weeks, 8 weeks, and 6 months of age (MGI Ref ID J:66102)
- increased left ventricle systolic pressure
- greater than 10% increase in left ventricular systolic pressure (MGI Ref ID J:66102)
- thick ventricular wall
- there is about a 50% increase in septal thickness and left ventricular posterior wall thickness (MGI Ref ID J:66102)
- muscle phenotype
- decreased cardiomyocyte apoptosis
- less apoptosis is observed in mice subjected to ischemia/reperfusion of the left coronary artery (MGI Ref ID J:66102)
- enlarged myocardial fiber
- about 30% increase in the cross-sectional area of each myocardial fiber (MGI Ref ID J:66102)
- cellular phenotype
- decreased cardiomyocyte apoptosis
- less apoptosis is observed in mice subjected to ischemia/reperfusion of the left coronary artery (MGI Ref ID J:66102)
Tg(Myh6-Map2k1*)1Jmol/?
involves: FVB/N
- cardiovascular system phenotype
- abnormal cardiac output
- 7 days after ischemia injury, mice have no loss of cardiac output compared to 50% loss in wild-type controls (MGI Ref ID J:127872)
- decreased cardiomyocyte apoptosis
- eight fold less cardiomyocytes are undergoing apoptosis 24 hours after ischemia/reperfusion injury (MGI Ref ID J:127872)
- decreased myocardial infarction size
- there is two-third less infarction of the left ventricular area after ischemia/reperfusion injury (MGI Ref ID J:127872)
- muscle phenotype
- decreased cardiomyocyte apoptosis
- eight fold less cardiomyocytes are undergoing apoptosis 24 hours after ischemia/reperfusion injury (MGI Ref ID J:127872)
- homeostasis/metabolism phenotype
- decreased myocardial infarction size
- there is two-third less infarction of the left ventricular area after ischemia/reperfusion injury (MGI Ref ID J:127872)
- cellular phenotype
- decreased cardiomyocyte apoptosis
- eight fold less cardiomyocytes are undergoing apoptosis 24 hours after ischemia/reperfusion injury (MGI Ref ID J:127872)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Heart Abnormalities
cardiomyopathy
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Myh6-Map2k1*)1Jmol | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffery D Molkentin | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | MEK1; MEK1 TG; | ||
| Mutation Made By | Jeffery Molkentin, Children's Hospital Medical Center | ||
| Strain of Origin | FVB | ||
| Expressed Gene | Map2k1, mitogen-activated protein kinase kinase 1, mouse, laboratory | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine | ||
| Molecular Note | A Map2k1 gene that encodes a constitutively active protein by substituting glutamines for serines 217 and 221 was put under the control of the cardiac specific Myh6 promoter. Nine independent lines were created with transgene copy number ranging from 2 to over 40. Transgene expression was detected in heart muscle at levels 3.9 to 6.3 fold higher than expression of endogenous Map2k1. This is a representative record for all lines. [MGI Ref ID J:66102] | ||
References
References provided by MGI
Selected Reference(s)
Bueno OF; De Windt LJ; Tymitz KM; Witt SA; Kimball TR; Klevitsky R; Hewett TE; Jones SP; Lefer DJ; Peng CF; Kitsis RN; Molkentin JD. 2000. The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice EMBO J 19(23):6341-50. [PubMed: 11101507] [MGI Ref ID J:66102]
Additional References
Tg(Myh6-Map2k1*)1Jmol relatedLips DJ; Bueno OF; Wilkins BJ; Purcell NH; Kaiser RA; Lorenz JN; Voisin L; Saba-El-Leil MK; Meloche S; Pouyssegur J; Pages G; De Windt LJ; Doevendans PA; Molkentin JD. 2004. MEK1-ERK2 signaling pathway protects myocardium from ischemic injury in vivo. Circulation 109(16):1938-41. [PubMed: 15096454] [MGI Ref ID J:127872]
Maejima Y; Galeotti J; Molkentin JD; Sadoshima J; Zhai P. 2012. Constitutively active MEK1 rescues cardiac dysfunction caused by overexpressed GSK-3alpha during aging and hemodynamic pressure overload. Am J Physiol Heart Circ Physiol 303(8):H979-88. [PubMed: 22904158] [MGI Ref ID J:191817]
Purcell NH; Darwis D; Bueno OF; Muller JM; Schule R; Molkentin JD. 2004. Extracellular signal-regulated kinase 2 interacts with and is negatively regulated by the LIM-only protein FHL2 in cardiomyocytes. Mol Cell Biol 24(3):1081-95. [PubMed: 14729955] [MGI Ref ID J:149730]
Purcell NH; Wilkins BJ; York A; Saba-El-Leil MK; Meloche S; Robbins J; Molkentin JD. 2007. Genetic inhibition of cardiac ERK1/2 promotes stress-induced apoptosis and heart failure but has no effect on hypertrophy in vivo. Proc Natl Acad Sci U S A 104(35):14074-9. [PubMed: 17709754] [MGI Ref ID J:124903]
van Berlo JH; Elrod JW; Aronow BJ; Pu WT; Molkentin JD. 2011. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A 108(30):12331-6. [PubMed: 21746915] [MGI Ref ID J:174537]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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