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| The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ser189/193Ala mutant mitogen-activated protein kinase kinase 3 (Map2k3). These mice develop a progressive, severe hypertrophic cardiomyopathy detected at two months of age. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Jeffery Molkentin, Children's Hospital Medical Center Description
The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ser189/193Ala mutant mitogen-activated protein kinase kinase 3 (Map2k3). These mice develop a progressive, severe hypertrophic cardiomyopathy detected at two months of age. Histological staining reveals interstitial cell fibrosis and a significant increase in myocyte cross-sectional area by 4 months of age.Development
The mouse cardiac alpha myosin heavy chain (Myh6) promoter drives expression of a dominant negative Ser189Ala/Ser193Ala form of mouse MKK3 (Map2k3). The strain was created and maintained on an FVB background by the donating laboratory.
Related Strains
Strains carrying other alleles of Map2k3
006416 B6;129S1-Map2k3tm1Flv/J View Strains carrying other alleles of Map2k3 (1 strain)
Phenotype
Phenotype Information
assigned by genotype
Tg(Myh6-Map2k3*)1Jmol/0
FVB-Tg(Myh6-Map2k3*)1Jmol
- mortality/aging
- premature death
- most die by 8 months resulting from lethal cardiomyopathy (MGI Ref ID J:83547)
- cardiovascular system phenotype
- abnormal heart left ventricle morphology
- thinning of left ventricular free wall and septum are observed at later time points (MGI Ref ID J:83547)
- heart left ventricle hypertrophy
- end-diastolic and end-systolic dimensions are significantly increased, with unchanged or decreased wall thickness suggesting cardiomyopathy (MGI Ref ID J:83547)
- cardiac hypertrophy
- at 2 months, mice show significant increase in heart-to-body weight ratios that increase with age compared to controls (MGI Ref ID J:83547)
- before 2 months of age, mice exhibit concentric hypertrophic remodeling (MGI Ref ID J:83547)
- at 2 months, mice show greater than 2-fold increase in heart-to-body weight ratio compared to sham controls induced by 14 days of aortic banding; mice also display a significantly greater percent increase in heart-to-body weight versus controls after 14 days of infusion of angiotensin II, isoproterenol, or phenylephrine (MGI Ref ID J:83547)
- heart left ventricle hypertrophy
- end-diastolic and end-systolic dimensions are significantly increased, with unchanged or decreased wall thickness suggesting cardiomyopathy (MGI Ref ID J:83547)
- cardiac interstitial fibrosis
- insterstitial cell fibrosis is observed by 4 months of age (MGI Ref ID J:83547)
- cardiomyopathy (MGI Ref ID J:83547)
- decreased cardiac muscle contractility
- by 4 months, fractional shortening is significantly reduced (MGI Ref ID J:83547)
- decreased ventricle muscle contractility
- left ventricular pressure gradient versus time (systolic function) is significantly reduced by 4 months of age (MGI Ref ID J:83547)
- enlarged myocardial fiber
- myocyte cross-sectional area is increased significantly by 4 months of age (MGI Ref ID J:83547)
- muscle phenotype
- cardiomyopathy (MGI Ref ID J:83547)
- decreased cardiac muscle contractility
- by 4 months, fractional shortening is significantly reduced (MGI Ref ID J:83547)
- decreased ventricle muscle contractility
- left ventricular pressure gradient versus time (systolic function) is significantly reduced by 4 months of age (MGI Ref ID J:83547)
- enlarged myocardial fiber
- myocyte cross-sectional area is increased significantly by 4 months of age (MGI Ref ID J:83547)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Heart Abnormalities
cardiomyopathy
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Myh6-Map2k3*)1Jmol | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffery D Molkentin | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | dnMKK3; | ||
| Mutation Made By | Jeffery Molkentin, Children's Hospital Medical Center | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Map2k3, mitogen-activated protein kinase kinase 3, mouse, laboratory | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine | ||
| Molecular Note | he mouse cardiac alpha myosin heavy chain (Myh6) promoter drives expression of a dominant negative Ser189Ala/Ser193Ala form of mouse MKK3 (Map2k3). Two lines were generated and one was chosen for further characterization. [MGI Ref ID J:83547] | ||
References
References provided by MGI
Selected Reference(s)
Braz JC; Bueno OF; Liang Q; Wilkins BJ; Dai YS; Parsons S; Braunwart J; Glascock BJ; Klevitsky R; Kimball TF; Hewett TE; Molkentin JD. 2003. Targeted inhibition of p38 MAPK promotes hypertrophic cardiomyopathy through upregulation of calcineurin-NFAT signaling. J Clin Invest 111(10):1475-86. [PubMed: 12750397] [MGI Ref ID J:83547]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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