Strain Name:

B6;129S-Jag1tm2Grid/J

Stock Number:

010618

Order this mouse

Availability:

Repository- Live

These floxed mutant mice possess loxP sites flanking exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the Jag1 gene. This strain may be useful for generating conditional mutations for studies of Notch signaling during development.

Description

Strain Information

Type Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   26-OCT-10
Specieslaboratory mouse
GenerationF?+F8 (12-DEC-13)
Generation Definitions
 
Donating Investigator Tom Gridley,   Maine Medical Center Research Institute

Description
These mice possess loxP sites on either side of exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s). The exon 4-deleted allele produces a nonfunctional JAG1 protein.

When bred to a strain with Cre recombinase expression during development in the telencephalon and discrete head structures, such as the otocyst (see Stock No. 006084 for example), this mutant mouse strain may be useful in studies of developmental inner ear defects.

When bred to a strain with Cre recombinase expression in endothelial cells during embryogenesis and adulthood (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of congenital heart defects and ductus arteriosus development.

When bred to mice carrying Tg(Wnt1-cre)11Rth (Stock No. 009107), Cre recombinase expression in the midbrain and developing neural tube results in a craniofacial phenotype characteristic of Alagille Syndrome.

Development
A FRT site flanked targeting vector containing a PGK-neo cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were crossed to C57BL/6J females. Heterozygotes were intercrossed to generate homozygotes. The mice were then crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (STOCK no. 003946) to remove the PGK-neo selection cassette. These mice no longer carry the Gt(ROSA)26Sortm1(FLP1)Dym allele. The mice were crossed to C57BL/6J once to establish the colony.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
007664   129S-Efnb1tm1Sor/J
000646   A/J
000647   A/WySnJ
005709   B6.129-Skitm1Cco/J
002619   B6.129-Tgfb3tm1Doe/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010546   B6.129S1-Jag2tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
002711   B6;129-Gabrb3tm1Geh/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
022358   B6;129S6-Rr23tm1Axvi/Mmjax
022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
002990   B6;129S7-Inhbatm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCeb/OveJ
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
003318   STOCK Shhtm1Amc/J
003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
008469   STOCK Wnt9btm1.2Amc/J
View Facebase: models     (61 strains)

Strains carrying other alleles of Jag1
010616   B6.129S1-Jag1tm1Grid/J
View Strains carrying other alleles of Jag1     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Alagille Syndrome 1; ALGS1
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Jagged 1; JAG1   (JAG1)
Tetralogy of Fallot; TOF   (JAG1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Jag1tm2Grid/Jag1tm2Grid Tg(Tek-cre)12Flv/0

        involves: C3H * C57BL/6   (conditional)
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • die at approximately E10.5   (MGI Ref ID J:166769)
  • cardiovascular system phenotype
  • abnormal vascular smooth muscle morphology
    • non-cell autonomous defects in vascular smooth muscle cell differentiation   (MGI Ref ID J:166769)
  • abnormal vitelline vascular remodeling
    • fail to remodel the primary vascular plexus of the yolk sac to form large blood vessels   (MGI Ref ID J:166769)
  • hemorrhage   (MGI Ref ID J:166769)
  • pericardial edema   (MGI Ref ID J:166769)
  • embryogenesis phenotype
  • abnormal vitelline vascular remodeling
    • fail to remodel the primary vascular plexus of the yolk sac to form large blood vessels   (MGI Ref ID J:166769)
  • homeostasis/metabolism phenotype
  • pericardial edema   (MGI Ref ID J:166769)
  • muscle phenotype
  • abnormal vascular smooth muscle morphology
    • non-cell autonomous defects in vascular smooth muscle cell differentiation   (MGI Ref ID J:166769)

Jag1tm2Grid/Jag1tm2Grid Tg(Wnt1-cre)11Rth/0

        involves: 129S1/Sv * C57BL/6 * CBA   (conditional)
  • mortality/aging
  • premature death
    • maxilla deficiencies lead to poor feeding and death around one month of age   (MGI Ref ID J:181120)
  • growth/size/body phenotype
  • abnormal palate morphology
    • the palatal dimensions are proportionally smaller, however the structural components are in tact   (MGI Ref ID J:181120)
    • mutants exhibit reduced proliferation in the palate shelves at E14.5 and in the adjacent palatal epithelium   (MGI Ref ID J:181120)
    • mutants exhibit reduced hyaluronic acid in the palate shelves at E14.5   (MGI Ref ID J:181120)
    • mutants exhibit aberrant vascular pattering in the palate including reduced vascular branching at E14.5 and E15.5   (MGI Ref ID J:181120)
    • abnormal primary palate development
      • at E14.5, palatal shelf elevation above the tongue occurs normally but palatal elongation is reduced anteriorly, whereas the posterior palate shelf length is unaffected   (MGI Ref ID J:181120)
      • mutants exhibit delayed palatal shelf elongation   (MGI Ref ID J:181120)
      • some E15.5 mutants show delayed palate shelf apposition and the persistence of the epithelial seam, however palate fusion occurs in all mutants   (MGI Ref ID J:181120)
    • short palate
      • palate shelf height is reduced in both the anterior and posterior regions   (MGI Ref ID J:181120)
  • decreased body size   (MGI Ref ID J:181120)
  • midface hypoplasia
    • midfacial hypoplasia resulting in a reduction in anterior facial width   (MGI Ref ID J:181120)
  • craniofacial phenotype
  • abnormal palate morphology
    • the palatal dimensions are proportionally smaller, however the structural components are in tact   (MGI Ref ID J:181120)
    • mutants exhibit reduced proliferation in the palate shelves at E14.5 and in the adjacent palatal epithelium   (MGI Ref ID J:181120)
    • mutants exhibit reduced hyaluronic acid in the palate shelves at E14.5   (MGI Ref ID J:181120)
    • mutants exhibit aberrant vascular pattering in the palate including reduced vascular branching at E14.5 and E15.5   (MGI Ref ID J:181120)
    • abnormal primary palate development
      • at E14.5, palatal shelf elevation above the tongue occurs normally but palatal elongation is reduced anteriorly, whereas the posterior palate shelf length is unaffected   (MGI Ref ID J:181120)
      • mutants exhibit delayed palatal shelf elongation   (MGI Ref ID J:181120)
      • some E15.5 mutants show delayed palate shelf apposition and the persistence of the epithelial seam, however palate fusion occurs in all mutants   (MGI Ref ID J:181120)
    • short palate
      • palate shelf height is reduced in both the anterior and posterior regions   (MGI Ref ID J:181120)
  • malocclusion
    • dental occlusion is misaligned with severe midface hypoplasia requiring trimming of the lower incisors weekly   (MGI Ref ID J:181120)
  • midface hypoplasia
    • midfacial hypoplasia resulting in a reduction in anterior facial width   (MGI Ref ID J:181120)
  • short maxilla
    • shortened maxillary regions; anterior-posterior facial length is normal at P0 but over time the mutants exhibit smaller maxillary lengths   (MGI Ref ID J:181120)
    • the inframaxillary length and the posterior-anterior length are reduced   (MGI Ref ID J:181120)
  • skeleton phenotype
  • malocclusion
    • dental occlusion is misaligned with severe midface hypoplasia requiring trimming of the lower incisors weekly   (MGI Ref ID J:181120)
  • short maxilla
    • shortened maxillary regions; anterior-posterior facial length is normal at P0 but over time the mutants exhibit smaller maxillary lengths   (MGI Ref ID J:181120)
    • the inframaxillary length and the posterior-anterior length are reduced   (MGI Ref ID J:181120)
  • behavior/neurological phenotype
  • abnormal eating behavior
    • mutants exhibit poor feeding after 30 days of age due to malocclusion and require soft mouse chow   (MGI Ref ID J:181120)
  • cardiovascular system phenotype
  • abnormal blood vessel morphology
    • mutants exhibit aberrant vascular pattering in the palate; reduced vascular branching in the palate at E14.5 and E15.5, reduced vasculature organization and vessel size, poor vascular smooth muscle investment, and irregular vessel formation   (MGI Ref ID J:181120)
  • digestive/alimentary phenotype
  • abnormal palate morphology
    • the palatal dimensions are proportionally smaller, however the structural components are in tact   (MGI Ref ID J:181120)
    • mutants exhibit reduced proliferation in the palate shelves at E14.5 and in the adjacent palatal epithelium   (MGI Ref ID J:181120)
    • mutants exhibit reduced hyaluronic acid in the palate shelves at E14.5   (MGI Ref ID J:181120)
    • mutants exhibit aberrant vascular pattering in the palate including reduced vascular branching at E14.5 and E15.5   (MGI Ref ID J:181120)
    • abnormal primary palate development
      • at E14.5, palatal shelf elevation above the tongue occurs normally but palatal elongation is reduced anteriorly, whereas the posterior palate shelf length is unaffected   (MGI Ref ID J:181120)
      • mutants exhibit delayed palatal shelf elongation   (MGI Ref ID J:181120)
      • some E15.5 mutants show delayed palate shelf apposition and the persistence of the epithelial seam, however palate fusion occurs in all mutants   (MGI Ref ID J:181120)
    • short palate
      • palate shelf height is reduced in both the anterior and posterior regions   (MGI Ref ID J:181120)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Jag1tm2Grid
Allele Name targeted mutation 2, Tom Gridley
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Jag1flox;
Mutation Made By Tom Gridley,   Maine Medical Center Research Institute
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Jag1, jagged 1
Chromosome 2
Gene Common Name(s) ABE2; AGS; AHD; AWS; CD339; Gsfabe2; HJ1; Headturner; Htu; JAGL1; Ozz; Serrate-1; gsf abnormal behavior 2; headturner; ozzy;
Molecular Note Exon 4 was flanked by loxP sites. An FRT-flanked neo was removed from the locus. [MGI Ref ID J:115783]

Genotyping

Genotyping Information

Genotyping Protocols

Jag1tm2Grid, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kiernan AE; Xu J; Gridley T. 2006. The Notch ligand JAG1 is required for sensory progenitor development in the mammalian inner ear. PLoS Genet 2(1):e4. [PubMed: 16410827]  [MGI Ref ID J:115783]

Additional References

Jag1tm2Grid related

Feng X; Krebs LT; Gridley T. 2010. Patent ductus arteriosus in mice with smooth muscle-specific Jag1 deletion. Development 137(24):4191-9. [PubMed: 21068062]  [MGI Ref ID J:166769]

High FA; Jain R; Stoller JZ; Antonucci NB; Lu MM; Loomes KM; Kaestner KH; Pear WS; Epstein JA. 2009. Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development. J Clin Invest 119(7):1986-96. [PubMed: 19509466]  [MGI Ref ID J:152574]

High FA; Lu MM; Pear WS; Loomes KM; Kaestner KH; Epstein JA. 2008. Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development. Proc Natl Acad Sci U S A 105(6):1955-9. [PubMed: 18245384]  [MGI Ref ID J:143962]

Hill CR; Yuasa M; Schoenecker J; Goudy SL. 2014. Jagged1 is essential for osteoblast development during maxillary ossification. Bone 62:10-21. [PubMed: 24491691]  [MGI Ref ID J:207974]

Humphreys R; Zheng W; Prince LS; Qu X; Brown C; Loomes K; Huppert SS; Baldwin S; Goudy S. 2012. Cranial neural crest ablation of Jagged1 recapitulates the craniofacial phenotype of Alagille syndrome patients. Hum Mol Genet 21(6):1374-83. [PubMed: 22156581]  [MGI Ref ID J:181120]

Kode A; Manavalan JS; Mosialou I; Bhagat G; Rathinam CV; Luo N; Khiabanian H; Lee A; Murty VV; Friedman R; Brum A; Park D; Galili N; Mukherjee S; Teruya-Feldstein J; Raza A; Rabadan R; Berman E; Kousteni S. 2014. Leukaemogenesis induced by an activating beta-catenin mutation in osteoblasts. Nature 506(7487):240-4. [PubMed: 24429522]  [MGI Ref ID J:207492]

Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081]  [MGI Ref ID J:164383]

Zhou Y; Tanzie C; Yan Z; Chen S; Duncan M; Gaudenz K; Li H; Seidel C; Lewis B; Moran A; Libby RT; Kiernan AE; Xie T. 2013. Notch2 regulates BMP signaling and epithelial morphogenesis in the ciliary body of the mouse eye. Proc Natl Acad Sci U S A 110(22):8966-71. [PubMed: 23676271]  [MGI Ref ID J:197419]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   26-OCT-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Jag1tm2Grid  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Jag1tm2Grid x Homozygous for Jag1tm2Grid  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Jag1tm2Grid  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Jag1tm2Grid x Homozygous for Jag1tm2Grid  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)