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| These Jag1flox mutant mice may be useful in generating conditional mutations for studies of Notch signaling during development. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 26-OCT-10 Species laboratory mouse Donating Investigator Tom Gridley, Maine Medical Center Research Institute Description
These mice possess loxP sites on either side of exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s). The exon 4-deleted allele produces a nonfunctional JAG1 protein.When bred to a strain with Cre recombinase expression during development in the telencephalon and discrete head structures, such as the otocyst (see Stock No. 006084 for example), this mutant mouse strain may be useful in studies of developmental inner ear defects.
When bred to a strain with Cre recombinase expression in endothelial cells during embryogenesis and adulthood (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of congenital heart defects and ductus arteriosus development.
When bred to mice carrying Tg(Wnt1-cre)11Rth (Stock No. 009107), Cre recombinase expression in the midbrain and developing neural tube results in a craniofacial phenotype characteristic of Alagille Syndrome.
Development
A FRT site flanked targeting vector containing a PGK-neo cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were crossed to C57BL/6J females. Heterozygotes were intercrossed to generate homozygotes. The mice were then crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (STOCK no. 003946) to remove the PGK-neo selection cassette. These mice no longer carry the Gt(ROSA)26Sortm1(FLP1)Dym allele. The mice were crossed to C57BL/6J once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: models
View Facebase: models (58 strains)
010616 B6.129S1-Jag1tm1Grid/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Alagille Syndrome 1; ALGS1
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Jagged 1; JAG1 (JAG1)
Tetralogy of Fallot; TOF (JAG1)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Jag1tm2Grid/Jag1tm2Grid Tg(Tek-cre)12Flv/0
involves: C3H * C57BL/6 (conditional)
- mortality/aging
- complete embryonic lethality during organogenesis
- die at approximately E10.5 (MGI Ref ID J:166769)
- cardiovascular system phenotype
- abnormal vascular smooth muscle morphology
- non-cell autonomous defects in vascular smooth muscle cell differentiation (MGI Ref ID J:166769)
- abnormal vitelline vascular remodeling
- fail to remodel the primary vascular plexus of the yolk sac to form large blood vessels (MGI Ref ID J:166769)
- hemorrhage (MGI Ref ID J:166769)
- pericardial edema (MGI Ref ID J:166769)
- embryogenesis phenotype
- abnormal vitelline vascular remodeling
- fail to remodel the primary vascular plexus of the yolk sac to form large blood vessels (MGI Ref ID J:166769)
- homeostasis/metabolism phenotype
- pericardial edema (MGI Ref ID J:166769)
- muscle phenotype
- abnormal vascular smooth muscle morphology
- non-cell autonomous defects in vascular smooth muscle cell differentiation (MGI Ref ID J:166769)
Jag1tm2Grid/Jag1tm2Grid Tg(Wnt1-cre)11Rth/0
involves: 129S1/Sv * C57BL/6 * CBA (conditional)
- mortality/aging
- premature death
- maxilla deficiencies lead to poor feeding and death around one month of age (MGI Ref ID J:181120)
- growth/size phenotype
- decreased body size (MGI Ref ID J:181120)
- craniofacial phenotype
- abnormal palate morphology
- the palatal dimensions are proportionally smaller, however the structural components are in tact (MGI Ref ID J:181120)
- mutants exhibit reduced proliferation in the palate shelves at E14.5 and in the adjacent palatal epithelium (MGI Ref ID J:181120)
- mutants exhibit reduced hyaluronic acid in the palate shelves at E14.5 (MGI Ref ID J:181120)
- mutants exhibit aberrant vascular pattering in the palate including reduced vascular branching at E14.5 and E15.5 (MGI Ref ID J:181120)
- abnormal primary palate development
- at E14.5, palatal shelf elevation above the tongue occurs normally but palatal elongation is reduced anteriorly, whereas the posterior palate shelf length is unaffected (MGI Ref ID J:181120)
- mutants exhibit delayed palatal shelf elongation (MGI Ref ID J:181120)
- some E15.5 mutants show delayed palate shelf apposition and the persistence of the epithelial seam, however palate fusion occurs in all mutants (MGI Ref ID J:181120)
- short palate
- palate shelf height is reduced in both the anterior and posterior regions (MGI Ref ID J:181120)
- malocclusion
- dental occlusion is misaligned with severe midface hypoplasia requiring trimming of the lower incisors weekly (MGI Ref ID J:181120)
- midface retrusion
- midfacial hypoplasia resulting in a reduction in anterior facial width (MGI Ref ID J:181120)
- short maxilla
- skeleton phenotype
- malocclusion
- dental occlusion is misaligned with severe midface hypoplasia requiring trimming of the lower incisors weekly (MGI Ref ID J:181120)
- short maxilla
- behavior/neurological phenotype
- decreased eating behavior
- mutants exhibit poor feeding after 30 days of age due to malocclusion and require soft mouse chow (MGI Ref ID J:181120)
- cardiovascular system phenotype
- abnormal blood vessel morphology
- mutants exhibit aberrant vascular pattering in the palate; reduced vascular branching in the palate at E14.5 and E15.5, reduced vasculature organization and vessel size, poor vascular smooth muscle investment, and irregular vessel formation (MGI Ref ID J:181120)
- digestive/alimentary phenotype
- abnormal palate morphology
- the palatal dimensions are proportionally smaller, however the structural components are in tact (MGI Ref ID J:181120)
- mutants exhibit reduced proliferation in the palate shelves at E14.5 and in the adjacent palatal epithelium (MGI Ref ID J:181120)
- mutants exhibit reduced hyaluronic acid in the palate shelves at E14.5 (MGI Ref ID J:181120)
- mutants exhibit aberrant vascular pattering in the palate including reduced vascular branching at E14.5 and E15.5 (MGI Ref ID J:181120)
- abnormal primary palate development
- at E14.5, palatal shelf elevation above the tongue occurs normally but palatal elongation is reduced anteriorly, whereas the posterior palate shelf length is unaffected (MGI Ref ID J:181120)
- mutants exhibit delayed palatal shelf elongation (MGI Ref ID J:181120)
- some E15.5 mutants show delayed palate shelf apposition and the persistence of the epithelial seam, however palate fusion occurs in all mutants (MGI Ref ID J:181120)
- short palate
- palate shelf height is reduced in both the anterior and posterior regions (MGI Ref ID J:181120)
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Jag1tm2Grid | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Tom Gridley | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Jag1flox; | ||
| Mutation Made By | Tom Gridley, Maine Medical Center Research Institute | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Gene Symbol and Name | Jag1, jagged 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | ABE2; AGS; AHD; AWS; CD339; Gsfabe2; HJ1; Headturner; Htu; JAGL1; Ozz; Serrate-1; gsf abnormal behavior 2; headturner; ozzy; | ||
| Molecular Note | Exon 4 was flanked by loxP sites. An FRT-flanked neo was removed from the locus. [MGI Ref ID J:115783] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Jag1tm2Grid, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kiernan AE; Xu J; Gridley T. 2006. The Notch ligand JAG1 is required for sensory progenitor development in the mammalian inner ear. PLoS Genet 2(1):e4. [PubMed: 16410827] [MGI Ref ID J:115783]
Additional References
Jag1tm2Grid relatedFeng X; Krebs LT; Gridley T. 2010. Patent ductus arteriosus in mice with smooth muscle-specific Jag1 deletion. Development 137(24):4191-9. [PubMed: 21068062] [MGI Ref ID J:166769]
High FA; Jain R; Stoller JZ; Antonucci NB; Lu MM; Loomes KM; Kaestner KH; Pear WS; Epstein JA. 2009. Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development. J Clin Invest 119(7):1986-96. [PubMed: 19509466] [MGI Ref ID J:152574]
High FA; Lu MM; Pear WS; Loomes KM; Kaestner KH; Epstein JA. 2008. Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development. Proc Natl Acad Sci U S A 105(6):1955-9. [PubMed: 18245384] [MGI Ref ID J:143962]
Humphreys R; Zheng W; Prince LS; Qu X; Brown C; Loomes K; Huppert SS; Baldwin S; Goudy S. 2012. Cranial neural crest ablation of Jagged1 recapitulates the craniofacial phenotype of Alagille syndrome patients. Hum Mol Genet 21(6):1374-83. [PubMed: 22156581] [MGI Ref ID J:181120]
Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081] [MGI Ref ID J:164383]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 26-OCT-10 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $195.00 Female or Male Homozygous for Jag1tm2Grid
Price per Pair (US dollars $) Pair Genotype $390.00 Homozygous for Jag1tm2Grid x Homozygous for Jag1tm2Grid Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $253.50 Female or Male Homozygous for Jag1tm2Grid
Price per Pair (US dollars $) Pair Genotype $507.00 Homozygous for Jag1tm2Grid x Homozygous for Jag1tm2Grid Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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|
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Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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