Strain Name:

NOD/ShiLt-Tg(INS-NP)6171Mvh/J

Stock Number:

010632

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Availability:

Cryopreserved - Ready for recovery

These NOD mice are hemizygous for the Lymphocytic choriomeningitis virus nucleoprotein under the control of human insulin promoter (HIP). This model provides a novel autoantigen expressed in pancreatic islet cells. LCMV infected transgenic mice provide an inducible system of early onset diabetes.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Background Strain NOD/ShiLtJ
H2 Haplotypeg7
 
Donating InvestigatorDr. Matthais von Herrath,   La Jolla Institute for Allergy and Immun

Appearance
albino
Related Genotype: A/A Tyrc/Tyrc

Description
Transgenic mice were created with the Lymphocytic choriomeningitis virus (LCMV) nucleoprotein(NP) under the control of the human insulin promoter (HIP). RT-PCR analysis indicates that transgenic mice express LCMV-nucleoprotein in the pancreas and thymus. Naive transgenic female mice exhibit slightly accelerated diabetes onset (95% diabetic by 35 weeks of age) when compared to wildtype controls (80% diabetic by 35 weeks of age), with no significant difference in overall diabetes incidence by 40 weeks of age. Immunohistochemistry of pancreas at one-week post LCMV infection shows an early infiltration of CD8 T cells in transgenic mice. Within 2 weeks of infection with LCMV HIP-NP mice exhibit rapid onset of diabetes compared to 6-8 months in infected B6.RIP-NP (Stock No. 004826) mice. LCMV infected transgenic mice exhibit upregulation of CXCR3 and CXCR10. This model provides a novel autoantigen expressed in pancreatic islet cells. LCMV infected transgenic mice provide an inducible system of early onset diabetes. This LCMV inducible strain may provide a model for early onset diabetes.

Development
NOD/ShiLt-Tg(INS-NP)6171Mvh/J expresses the complete cDNA from Armstrong's clone 53b of the lymphocytic choriomeningitis virus, LCMV, nucleoprotein, NP, regulated by human insulin promoter, INS, commonly referred to as HIP. A Hepatitis B Virus polyadenylation signal as a terminator of transcription was fused downstream of the LCMV-NP. This transgene was microinjected into NOD/ShiLt oocytes and progeny of founder 6171, have been maintained on the NOD/ShiLtJ (Stock No. 001976) background (Martinic et al, 2007). In 2010, this strain arrived at The Jackson Laboratory and was backcrossed to NOD/ShiLtJ.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of INS
006411   B6.129-Gasttm1(INS)Ez/J
017570   C.Cg-Tg(INS)1Gdmn/J
005115   NOD.FVB-Tg(INS-MT2A,Tyr)1Pne/PneJ
005113   NOD.FVB-Tg(INS-SOD2)3Pne/PneJ
View Strains carrying other alleles of INS     (4 strains)

Strains carrying other alleles of NP
004826   B6.Cg-Tg(Ins2-NP)25-3Olds/MhvJ
004827   C.Cg-Tg(Ins2-NP)25-3Olds/MvhJ
View Strains carrying other alleles of NP     (2 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      Type 1 Diabetes

Research Tools
Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research
      T Cell Receptor Transgenics

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(INS-NP)6171Mvh
Allele Name transgene insertion 6171, Matthias von Herrath
Allele Type Transgenic (random, expressed)
Common Name(s) Ins-LCMV-NP; LCMV-NP;
Strain of OriginNOD/ShiLt
Expressed Gene NP, lymphocytic choriomeningitis virus nucleoprotein, viral
Promoter INS, insulin, human
Molecular Note The transgene expresses the complete cDNA from Armstrong's clone 53b of the lymphocytic choriomeningitis virus, LCMV, nucleoprotein, NP, regulated by human insulin promoter, INS. A Hepatitis B Virus polyadenylation signal as a terminator of transcription was fused downstream of the LCMV-NP. This transgene was microinjected into NOD/ShiLt oocytes and founder line 6171 was maintained on the NOD/ShiLt background. [MGI Ref ID J:122080]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Ins2-NP)25-3Olds, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Martinic MM; Juedes AE; Bresson D; Homann D; Skak K; Huber C; Ling E; Ejrnaes M; Wolfe T; Togher L; Christen U; von Herrath MG. 2007. Minimal impact of a de novo-expressed beta-cell autoantigen on spontaneous diabetes development in NOD mice. Diabetes 56(4):1059-68. [PubMed: 17395746]  [MGI Ref ID J:122080]

Additional References

Tg(INS-NP)6171Mvh related

Van Belle TL; Nierkens S; Arens R; von Herrath MG. 2012. Interleukin-21 receptor-mediated signals control autoreactive T cell infiltration in pancreatic islets. Immunity 36(6):1060-72. [PubMed: 22579473]  [MGI Ref ID J:187322]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain is maintained +/+ x hemizygote.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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