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| These NOD mice are hemizygous for the Lymphocytic choriomeningitis virus nucleoprotein under the control of human insulin promoter (HIP). This model provides a novel autoantigen expressed in pancreatic islet cells. LCMV infected transgenic mice provide an inducible system of early onset diabetes. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Background Strain NOD/ShiLtJ H2 Haplotype g7 Donating Investigator Dr. Matthais von Herrath, La Jolla Institute for Allergy and Immun Appearance
albino
Related Genotype: A/A Tyrc/TyrcDescription
Transgenic mice were created with the Lymphocytic choriomeningitis virus (LCMV) nucleoprotein(NP) under the control of the human insulin promoter (HIP). RT-PCR analysis indicates that transgenic mice express LCMV-nucleoprotein in the pancreas and thymus. Naive transgenic female mice exhibit slightly accelerated diabetes onset (95% diabetic by 35 weeks of age) when compared to wildtype controls (80% diabetic by 35 weeks of age), with no significant difference in overall diabetes incidence by 40 weeks of age. Immunohistochemistry of pancreas at one-week post LCMV infection shows an early infiltration of CD8 T cells in transgenic mice. Within 2 weeks of infection with LCMV HIP-NP mice exhibit rapid onset of diabetes compared to 6-8 months in infected B6.RIP-NP (Stock No. 004826) mice. LCMV infected transgenic mice exhibit upregulation of CXCR3 and CXCR10. This model provides a novel autoantigen expressed in pancreatic islet cells. LCMV infected transgenic mice provide an inducible system of early onset diabetes. This LCMV inducible strain may provide a model for early onset diabetes.Development
NOD/ShiLt-Tg(INS-NP)6171Mvh/J expresses the complete cDNA from Armstrong's clone 53b of the lymphocytic choriomeningitis virus, LCMV, nucleoprotein, NP, regulated by human insulin promoter, INS, commonly referred to as HIP. A Hepatitis B Virus polyadenylation signal as a terminator of transcription was fused downstream of the LCMV-NP. This transgene was microinjected into NOD/ShiLt oocytes and progeny of founder 6171, have been maintained on the NOD/ShiLtJ (Stock No. 001976) background (Martinic et al, 2007). In 2010, this strain arrived at The Jackson Laboratory and was backcrossed to NOD/ShiLtJ.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of INS
006411 B6.129-Gasttm1(INS)Ez/J 017570 C.Cg-Tg(INS)1Gdmn/J 005115 NOD.FVB-Tg(INS-MT2A,Tyr)1Pne/PneJ 005113 NOD.FVB-Tg(INS-SOD2)3Pne/PneJ View Strains carrying other alleles of INS (4 strains)
004826 B6.Cg-Tg(Ins2-NP)25-3Olds/MhvJ 004827 C.Cg-Tg(Ins2-NP)25-3Olds/MvhJ
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Type 1 Diabetes
Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research
T Cell Receptor Transgenics
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(INS-NP)6171Mvh | ||
|---|---|---|---|
| Allele Name | transgene insertion 6171, Matthias von Herrath | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Ins-LCMV-NP; LCMV-NP; | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | NP, lymphocytic choriomeningitis virus nucleoprotein, viral | ||
| Promoter | INS, insulin, human | ||
| Molecular Note | The transgene expresses the complete cDNA from Armstrong's clone 53b of the lymphocytic choriomeningitis virus, LCMV, nucleoprotein, NP, regulated by human insulin promoter, INS. A Hepatitis B Virus polyadenylation signal as a terminator of transcription was fused downstream of the LCMV-NP. This transgene was microinjected into NOD/ShiLt oocytes and founder line 6171 was maintained on the NOD/ShiLt background. [MGI Ref ID J:122080] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ins2-NP)25-3Olds, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Martinic MM; Juedes AE; Bresson D; Homann D; Skak K; Huber C; Ling E; Ejrnaes M; Wolfe T; Togher L; Christen U; von Herrath MG. 2007. Minimal impact of a de novo-expressed beta-cell autoantigen on spontaneous diabetes development in NOD mice. Diabetes 56(4):1059-68. [PubMed: 17395746] [MGI Ref ID J:122080]
Additional References
Tg(INS-NP)6171Mvh relatedVan Belle TL; Nierkens S; Arens R; von Herrath MG. 2012. Interleukin-21 receptor-mediated signals control autoreactive T cell infiltration in pancreatic islets. Immunity 36(6):1060-72. [PubMed: 22579473] [MGI Ref ID J:187322]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain is maintained +/+ x hemizygote.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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