Strain Name:

C;129S-Adora2atm1Jfc/J

Stock Number:

010685

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Availability:

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Mice that are homozygous for this knockout of the Adora2a gene, are resistant to experimentally induced ischemic brain injury and exhibit increased resistance to the addictive substances amphetamine and cocaine.

Description

Strain Information

Former Names C;129-Adora2atm1Jfc/J    (Changed: 20-OCT-09 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   27-JUL-10
Specieslaboratory mouse
GenerationN2F?+F11 (27-AUG-14)
Generation Definitions
 
Donating Investigator Joel Linden,   University of Virginia

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No functional gene product (protein) is detected by receptor autoradiography analysis of the brain. Experimentally induced cerebral infarction volume and resulting neurological function impairment are reduced in homozygotes. Treatment with receptor agonist, CGS 21680, does not elicit decreased locomotor activity in homozygotes, as compared to wildtype. Homozygotes have reduced spontaneous activity and increased resistance to the addictive substances amphetamine and cocaine. This mutant mouse strain may be useful in studies of behavior, ischemic brain injury, stroke, responses to addictive substances and T cell response to inflammation.

Development
A targeting vector containing PGKneo cassette was used to disrupt the 3' portion of exon 2 and intron 2. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129/SvEvTac mice, and then been backcrossed to BALB/cJ for 2 generations (using a speed congenic protocol) before arriving at The Jackson Laboratory. The mice were crossed to BALB/cJ once to establish the colony.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Adora2a
017863   B6.Cg-Tg(Adora2a-Chrm3*,-mCherry)AD6Blr/J
010687   B6;129-Adora2atm1Dyj/J
View Strains carrying other alleles of Adora2a     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Pulmonary Hypertension, Primary, 1; PPH1
Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.
Myopia 2, Autosomal Dominant; MYP2
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Adora2atm1Jfc/Adora2atm1Jfc

        involves: 129S4/SvJae * C57BL/6
  • behavior/neurological phenotype
  • hypoactivity   (MGI Ref ID J:73594)
    • reduced spontaneous activity most prominently in the dark cycle; however normal locomotor circadian rhythms are seen   (MGI Ref ID J:107988)
  • impaired behavioral response to addictive substance
    • no significant increase in wakefulness is seen in homozygotes after injection of 15 mg/kg, 10 mg/kg, or 5 mg/kg caffeine, unlike wild-type mice   (MGI Ref ID J:99612)
    • however, baseline circadian sleep-wake profiles are identical to wild-type   (MGI Ref ID J:99612)
    • single exposure amphetamine or cocaine stimulated increases in locomotor activity are reduced compared to wild-type mice   (MGI Ref ID J:107988)
    • however, amphetamine stimulated increases in fine movement are not significantly different from wild-type   (MGI Ref ID J:107988)
  • homeostasis/metabolism phenotype
  • decreased susceptibility to ischemic brain injury
    • 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice   (MGI Ref ID J:73594)
    • decreased cerebral infarction size
      • after middle cerebral arterial occlusion total infarct volume is reduced by 26% using hematoxylin and eosin staining or by 77% using TTC staining (marker of intact cellular metabolism) compared to wild-type mice   (MGI Ref ID J:73594)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • morphology of cortex and striatum appears normal   (MGI Ref ID J:73594)
    • dopaminergic innervation is normal   (MGI Ref ID J:107988)
    • decreased susceptibility to ischemic brain injury
      • 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice   (MGI Ref ID J:73594)
      • decreased cerebral infarction size
        • after middle cerebral arterial occlusion total infarct volume is reduced by 26% using hematoxylin and eosin staining or by 77% using TTC staining (marker of intact cellular metabolism) compared to wild-type mice   (MGI Ref ID J:73594)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • basal mean arterial blood pressure, absolute blood flow, and cortical cerebral blood flow before, during. and after middle cerebral arterial occlusion is similar to wild-type mice   (MGI Ref ID J:73594)
  • vision/eye phenotype
  • *normal* vision/eye phenotype
    • normal growth in the anterior segment and normal pupil size   (MGI Ref ID J:164077)
    • abnormal sclera morphology
      • sclera contains denser collagen fibrils with reduced diameter   (MGI Ref ID J:164077)
    • myopia
      • mice develop greater myopia than wild-type mice, that is associated with increases in vitreous chamber depth and axial length from P28 to P56   (MGI Ref ID J:164077)

Adora2atm1Jfc/Adora2atm1Jfc

        involves: 129S4/SvJae * 129S6/SvEvTac
  • behavior/neurological phenotype
  • impaired behavioral response to addictive substance
    • homozygotes do not display enhanced locomotor activation (over activation seen after the first exposure) after repeated administration of amphetamines   (MGI Ref ID J:106215)
    • however, locomotor responses to dopamine receptor D1A and dopamine receptor 2 agonists is similar to wild-type mice   (MGI Ref ID J:106215)
    • single exposure amphetamine stimulated increases in locomotor activity are reduced compared to wild-type mice   (MGI Ref ID J:107988)
  • nervous system phenotype
  • decreased susceptibility to ischemic brain injury
    • 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice   (MGI Ref ID J:73594)
    • decreased cerebral infarction size
      • after middle cerebral arterial occlusion total infarct volume is reduced by 30% compared to wild-type mice   (MGI Ref ID J:73594)
      • after middle cerebral arterial occlusion infarct volume in the cerebral cortex and striatum are reduced by 33% and 27%, respectively   (MGI Ref ID J:73594)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • basal mean arterial blood pressure, absolute blood flow, and cortical cerebral blood flow before, during. and after middle cerebral arterial occlusion is similar to wild-type mice   (MGI Ref ID J:73594)
  • homeostasis/metabolism phenotype
  • decreased physiological sensitivity to xenobiotic
    • homozygotes do not display increased expression of dynorphin mRNA in the striatum after repeated administration of amphetamines   (MGI Ref ID J:106215)
  • decreased susceptibility to ischemic brain injury
    • 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice   (MGI Ref ID J:73594)
    • decreased cerebral infarction size
      • after middle cerebral arterial occlusion total infarct volume is reduced by 30% compared to wild-type mice   (MGI Ref ID J:73594)
      • after middle cerebral arterial occlusion infarct volume in the cerebral cortex and striatum are reduced by 33% and 27%, respectively   (MGI Ref ID J:73594)

Adora2atm1Jfc/Adora2atm1Jfc

        involves: 129 * C57BL/6
  • behavior/neurological phenotype
  • abnormal locomotor activation
    • mutants do not show lower levels of locomotor stimulation in response to a Grm5 antagonist, MPEP, whereas compared to wild-type controls   (MGI Ref ID J:102700)

Adora2atm1Jfc/Adora2atm1Jfc

        B6.129S4-Adora2atm1Jfc
  • cardiovascular system phenotype
  • abnormal lung vasculature morphology
    • mutants exhibit hypertrophy of pulmonary resistance vessels with increased wall thickness and area   (MGI Ref ID J:194389)
    • mice exhibit evidence of pulmonary vascular remodeling, showing fibroblast, smooth muscle and endothelium cell hypertrophy   (MGI Ref ID J:194389)
    • however, mutants do not exhibit pulmonary edema, lung inflammation, fibrosis or thickening of the alveolar septa   (MGI Ref ID J:194389)
  • abnormal pulmonary artery morphology
    • cell proliferation is increased in all major cell types across entire pulmonary arterial walls, including smooth muscle, endothelium cells and fibroblasts compared to wild-type mice   (MGI Ref ID J:194389)
    • pulmonary arteries exhibit swelling and hypertrophy of endothelial and smooth muscle cells, with abundance of cytoplasm and an increase in intracytoplasmic vesicles   (MGI Ref ID J:194389)
    • pulmonary artery endothelium contains more Weibel-Palade bodies than in wild-type, indicating activation of endothelial cells   (MGI Ref ID J:194389)
    • cytoplasm of pulmonary artery smooth muscle cells contains numerous filaments and dense bodies, indicating activation of smooth muscle cells   (MGI Ref ID J:194389)
    • mutants exhibit enhanced hyperplasia of pulmonary artery fibroblasts, as indicated by an increase in fibroblasts seen outside the external elastic laminae and more clustered collagaen fibers deposition in adventitia pulmonary arterial walls   (MGI Ref ID J:194389)
  • heart right ventricle hypertrophy
    • the mean Fulton index (ratio of right ventricle over left ventricle plus septum) is higher in mutants than wild-type mice at 14-16 weeks of age, indicating hypertrophic right ventricles   (MGI Ref ID J:194389)
  • increased right ventricle systolic pressure
    • mice exhibit a 44.8% increase in right ventricular systolic pressure at 14-16 weeks of age   (MGI Ref ID J:194389)
    • however, mean systolic blood pressure and heart rate are normal   (MGI Ref ID J:194389)
    • following chronic exposure to hypoxia, mutants show an exacerbated elevation in right ventricular systolic pressure   (MGI Ref ID J:194389)
  • pulmonary hypertension
    • mice develop pulmonary arterial hypertension without affecting systemic circulation or heart rate   (MGI Ref ID J:194389)
    • following chronic exposure to hypoxia, mutants show exacerbated elevation in right ventricular systolic pressure, hypertrophy of pulmonary resistance vessels, and increased cell proliferation in pulmonary resistance vessels compared to wild-type mice, indicating a further increase in pulmonary hypertension under hypoxic conditions   (MGI Ref ID J:194389)
    • however, mutants do not show any features of hypertensive nephropathy   (MGI Ref ID J:194389)
  • vascular smooth muscle hypertrophy
    • pulmonary artery wall exhibits increased smooth muscle cell hypertorphy   (MGI Ref ID J:194389)
  • muscle phenotype
  • vascular smooth muscle hypertrophy
    • pulmonary artery wall exhibits increased smooth muscle cell hypertorphy   (MGI Ref ID J:194389)
  • respiratory system phenotype
  • abnormal lung vasculature morphology
    • mutants exhibit hypertrophy of pulmonary resistance vessels with increased wall thickness and area   (MGI Ref ID J:194389)
    • mice exhibit evidence of pulmonary vascular remodeling, showing fibroblast, smooth muscle and endothelium cell hypertrophy   (MGI Ref ID J:194389)
    • however, mutants do not exhibit pulmonary edema, lung inflammation, fibrosis or thickening of the alveolar septa   (MGI Ref ID J:194389)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Ischemia studies

Immunology, Inflammation and Autoimmunity Research
Inflammation

Neurobiology Research
Behavioral and Learning Defects

Research Tools
Immunology, Inflammation and Autoimmunity Research
      T cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Adora2atm1Jfc
Allele Name targeted mutation 1, Jiang-Fan Chen
Allele Type Targeted (Null/Knockout)
Common Name(s) A2A; A2A KO; A2AAR-; A2AR KO; A2aR-; Adora2atm1Chren; gKO;
Mutation Made By Vanessa Hajzus,   University of Virginia
Strain of Origin129S4/SvJae
Gene Symbol and Name Adora2a, adenosine A2a receptor
Chromosome 10
Gene Common Name(s) A2AAR; A2a, Rs; A2aR; AA2AR; ADORA2; RDC8;
Molecular Note Part of exon 2 and intron 2 was replaced by a neomycin selection cassette. The absence of functional protein was demonstrated in homozygous mice by in situ binding assays and neurochemical tests. [MGI Ref ID J:73594]

Genotyping

Genotyping Information

Genotyping Protocols

Adora2atm1Jfc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chen JF; Huang Z; Ma J; Zhu J; Moratalla R; Standaert D; Moskowitz MA; Fink JS; Schwarzschild MA. 1999. A(2A) adenosine receptor deficiency attenuates brain injury induced by transient focal ischemia in mice. J Neurosci 19(21):9192-200. [PubMed: 10531422]  [MGI Ref ID J:73594]

Additional References

Adora2atm1Jfc related

Apasov S; Chen JF; Smith P; Sitkovsky M. 2000. A(2A) receptor dependent and A(2A) receptor independent effects of extracellular adenosine on murine thymocytes in conditions of adenosine deaminase deficiency Blood 95(12):3859-67. [PubMed: 10845921]  [MGI Ref ID J:63086]

Bilbao A; Cippitelli A; Martin AB; Granado N; Ortiz O; Bezard E; Chen JF; Navarro M; Rodriguez de Fonseca F; Moratalla R. 2006. Absence of quasi-morphine withdrawal syndrome in adenosine A2A receptor knockout mice. Psychopharmacology (Berl) 185(2):160-8. [PubMed: 16470403]  [MGI Ref ID J:136509]

Cekic C; Sag D; Li Y; Theodorescu D; Strieter RM; Linden J. 2012. Adenosine A2B receptor blockade slows growth of bladder and breast tumors. J Immunol 188(1):198-205. [PubMed: 22116822]  [MGI Ref ID J:180439]

Chen JF; Beilstein M; Xu YH; Turner TJ; Moratalla R; Standaert DG; Aloyo VJ; Fink JS; Schwarzschild MA. 2000. Selective attenuation of psychostimulant-induced behavioral responses in mice lacking A(2A) adenosine receptors. Neuroscience 97(1):195-204. [PubMed: 10771351]  [MGI Ref ID J:107988]

Chen JF; Moratalla R; Impagnatiello F; Grandy DK; Cuellar B; Rubinstein M; Beilstein MA; Hackett E; Fink JS; Low MJ; Ongini E; Schwarzschild MA. 2001. The role of the D(2) dopamine receptor (D(2)R) in A(2A) adenosine receptor (A(2A)R)-mediated behavioral and cellular responses as revealed by A(2A) and D(2) receptor knockout mice. Proc Natl Acad Sci U S A 98(4):1970-5. [PubMed: 11172060]  [MGI Ref ID J:67550]

Chen JF; Moratalla R; Yu L; Martin AB; Xu K; Bastia E; Hackett E; Alberti I; Schwarzschild MA. 2003. Inactivation of adenosine A2A receptors selectively attenuates amphetamine-induced behavioral sensitization. Neuropsychopharmacology 28(6):1086-95. [PubMed: 12700712]  [MGI Ref ID J:106215]

Chia JS; McRae JL; Thomas HE; Fynch S; Elkerbout L; Hill P; Murray-Segal L; Robson SC; Chen JF; d'Apice AJ; Cowan PJ; Dwyer KM. 2013. The protective effects of CD39 overexpression in multiple low-dose streptozotocin-induced diabetes in mice. Diabetes 62(6):2026-35. [PubMed: 23364452]  [MGI Ref ID J:208556]

Dai SS; Li W; An JH; Wang H; Yang N; Chen XY; Zhao Y; Li P; Liu P; Chen JF; Zhou YG. 2010. Adenosine A2A receptors in both bone marrow cells and non-bone marrow cells contribute to traumatic brain injury. J Neurochem 113(6):1536-44. [PubMed: 20367749]  [MGI Ref ID J:163571]

Dai SS; Wang H; Yang N; An JH; Li W; Ning YL; Zhu PF; Chen JF; Zhou YG. 2013. Plasma glutamate-modulated interaction of A2AR and mGluR5 on BMDCs aggravates traumatic brain injury-induced acute lung injury. J Exp Med 210(4):839-51. [PubMed: 23478188]  [MGI Ref ID J:197541]

Day YJ; Huang L; McDuffie MJ; Rosin DL; Ye H; Chen JF; Schwarzschild MA; Fink JS; Linden J; Okusa MD. 2003. Renal protection from ischemia mediated by A2A adenosine receptors on bone marrow-derived cells. J Clin Invest 112(6):883-91. [PubMed: 12975473]  [MGI Ref ID J:117981]

Day YJ; Marshall MA; Huang L; McDuffie MJ; Okusa MD; Linden J. 2004. Protection from ischemic liver injury by activation of A2A adenosine receptors during reperfusion: inhibition of chemokine induction. Am J Physiol Gastrointest Liver Physiol 286(2):G285-93. [PubMed: 14715520]  [MGI Ref ID J:87604]

Deaglio S; Dwyer KM; Gao W; Friedman D; Usheva A; Erat A; Chen JF; Enjyoji K; Linden J; Oukka M; Kuchroo VK; Strom TB; Robson SC. 2007. Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. J Exp Med 204(6):1257-65. [PubMed: 17502665]  [MGI Ref ID J:125863]

Duan W; Gui L; Zhou Z; Liu Y; Tian H; Chen JF; Zheng J. 2009. Adenosine A2A receptor deficiency exacerbates white matter lesions and cognitive deficits induced by chronic cerebral hypoperfusion in mice. J Neurol Sci 285(1-2):39-45. [PubMed: 19524941]  [MGI Ref ID J:158143]

Duan W; Ran H; Zhou Z; He Q; Zheng J. 2012. Adenosine A2A receptor deficiency up-regulates cystatin F expression in white matter lesions induced by chronic cerebral hypoperfusion. PLoS One 7(12):e52566. [PubMed: 23285090]  [MGI Ref ID J:195759]

Figler RA; Wang G; Srinivasan S; Jung DY; Zhang Z; Pankow JS; Ravid K; Fredholm B; Hedrick CC; Rich SS; Kim JK; LaNoue KF; Linden J. 2011. Links between insulin resistance, adenosine A2B receptors, and inflammatory markers in mice and humans. Diabetes 60(2):669-79. [PubMed: 21270276]  [MGI Ref ID J:169783]

Fink JS; Kalda A; Ryu H; Stack EC; Schwarzschild MA; Chen JF; Ferrante RJ. 2004. Genetic and pharmacological inactivation of the adenosine A2A receptor attenuates 3-nitropropionic acid-induced striatal damage. J Neurochem 88(3):538-44. [PubMed: 14720203]  [MGI Ref ID J:90090]

Flach TL; Pang W; Chau EM; Desrosiers MD; Shi Y. 2009. Adenosine primes resting stage dendritic cells before their activation. Biochem Biophys Res Commun 380(4):748-51. [PubMed: 19338746]  [MGI Ref ID J:147345]

Fredduzzi S; Moratalla R; Monopoli A; Cuellar B; Xu K; Ongini E; Impagnatiello F; Schwarzschild MA; Chen JF. 2002. Persistent behavioral sensitization to chronic L-DOPA requires A2A adenosine receptors. J Neurosci 22(3):1054-62. [PubMed: 11826134]  [MGI Ref ID J:126975]

Gomez G; Sitkovsky MV. 2003. Differential requirement for A2a and A3 adenosine receptors for the protective effect of inosine in vivo. Blood 102(13):4472-8. [PubMed: 12947007]  [MGI Ref ID J:86917]

Gomez-Arroyo J; Saleem SJ; Mizuno S; Syed AA; Bogaard HJ; Abbate A; Taraseviciene-Stewart L; Sung Y; Kraskauskas D; Farkas D; Conrad DH; Nicolls MR; Voelkel NF. 2012. A brief overview of mouse models of pulmonary arterial hypertension: problems and prospects. Am J Physiol Lung Cell Mol Physiol 302(10):L977-91. [PubMed: 22307907]  [MGI Ref ID J:190182]

Grieder TE; George O; Tan H; George SR; Le Foll B; Laviolette SR; van der Kooy D. 2012. Phasic D1 and tonic D2 dopamine receptor signaling double dissociate the motivational effects of acute nicotine and chronic nicotine withdrawal. Proc Natl Acad Sci U S A 109(8):3101-6. [PubMed: 22308372]  [MGI Ref ID J:182622]

Gui L; Duan W; Tian H; Li C; Zhu J; Chen JF; Zheng J. 2009. Adenosine A 2A receptor deficiency reduces striatal glutamate outflow and attenuates brain injury induced by transient focal cerebral ischemia in mice. Brain Res 1297:185-93. [PubMed: 19703429]  [MGI Ref ID J:158556]

Huang JH; Cardenas-Navia LI; Caldwell CC; Plumb TJ; Radu CG; Rocha PN; Wilder T; Bromberg JS; Cronstein BN; Sitkovsky M; Dewhirst MW; Dustin ML. 2007. Requirements for T lymphocyte migration in explanted lymph nodes. J Immunol 178(12):7747-55. [PubMed: 17548612]  [MGI Ref ID J:148587]

Huang QY; Wei C; Yu L; Coelho JE; Shen HY; Kalda A; Linden J; Chen JF. 2006. Adenosine A2A receptors in bone marrow-derived cells but not in forebrain neurons are important contributors to 3-nitropropionic acid-induced striatal damage as revealed by cell-type-selective inactivation. J Neurosci 26(44):11371-8. [PubMed: 17079665]  [MGI Ref ID J:114707]

Huang ZL; Qu WM; Eguchi N; Chen JF; Schwarzschild MA; Fredholm BB; Urade Y; Hayaishi O. 2005. Adenosine A2A, but not A1, receptors mediate the arousal effect of caffeine. Nat Neurosci 8(7):858-9. [PubMed: 15965471]  [MGI Ref ID J:99612]

Kachroo A; Orlando LR; Grandy DK; Chen JF; Young AB; Schwarzschild MA. 2005. Interactions between metabotropic glutamate 5 and adenosine A2A receptors in normal and parkinsonian mice. J Neurosci 25(45):10414-9. [PubMed: 16280580]  [MGI Ref ID J:102700]

Katebi M; Soleimani M; Cronstein BN. 2009. Adenosine A2A receptors play an active role in mouse bone marrow-derived mesenchymal stem cell development. J Leukoc Biol 85(3):438-44. [PubMed: 19056861]  [MGI Ref ID J:146073]

Kiss I; Oskolas H; Toth R; Bouillet P; Toth K; Fulop A; Scholtz B; Ledent C; Fesus L; Szondy Z. 2006. Adenosine A2A receptor-mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77. Eur J Immunol 36(6):1559-71. [PubMed: 16673448]  [MGI Ref ID J:115069]

Lappas CM; Rieger JM; Linden J. 2005. A2A adenosine receptor induction inhibits IFN-gamma production in murine CD4+ T cells. J Immunol 174(2):1073-80. [PubMed: 15634932]  [MGI Ref ID J:95830]

Lee DJ; Taylor AW. 2013. Both MC5r and A2Ar are required for protective regulatory immunity in the spleen of post-experimental autoimmune uveitis in mice. J Immunol 191(8):4103-11. [PubMed: 24043903]  [MGI Ref ID J:206263]

Lee HT; Kim M; Joo JD; Gallos G; Chen JF; Emala CW. 2006. A3 adenosine receptor activation decreases mortality and renal and hepatic injury in murine septic peritonitis. Am J Physiol Regul Integr Comp Physiol 291(4):R959-69. [PubMed: 16728466]  [MGI Ref ID J:144900]

Leonard SK; Ferry-Leeper P; Mailman RB. 2006. Low affinity binding of the classical D(1) antagonist SCH23390 in rodent brain: Potential interaction with A(2A) and D(2)-like receptors. Brain Res 1117(1):25-37. [PubMed: 16962565]  [MGI Ref ID J:114543]

Li H; Zhang Z; Blackburn MR; Wang SW; Ribelayga CP; O'Brien J. 2013. Adenosine and dopamine receptors coregulate photoreceptor coupling via gap junction phosphorylation in mouse retina. J Neurosci 33(7):3135-50. [PubMed: 23407968]  [MGI Ref ID J:194258]

Li J; Zhao L; He X; Zeng YJ; Dai SS. 2013. Sinomenine protects against lipopolysaccharide-induced acute lung injury in mice via adenosine A(2A) receptor signaling. PLoS One 8(3):e59257. [PubMed: 23555007]  [MGI Ref ID J:199374]

Li L; Hao JX; Fredholm BB; Schulte G; Wiesenfeld-Hallin Z; Xu XJ. 2010. Peripheral adenosine A2A receptors are involved in carrageenan-induced mechanical hyperalgesia in mice. Neuroscience 170(3):923-8. [PubMed: 20678550]  [MGI Ref ID J:165291]

Li L; Huang L; Ye H; Song SP; Bajwa A; Lee SJ; Moser EK; Jaworska K; Kinsey GR; Day YJ; Linden J; Lobo PI; Rosin DL; Okusa MD. 2012. Dendritic cells tolerized with adenosine A(2)AR agonist attenuate acute kidney injury. J Clin Invest 122(11):3931-42. [PubMed: 23093781]  [MGI Ref ID J:194009]

Li W; Dai S; An J; Xiong R; Li P; Chen X; Zhao Y; Liu P; Wang H; Zhu P; Chen J; Zhou Y. 2009. Genetic inactivation of adenosine A2A receptors attenuates acute traumatic brain injury in the mouse cortical impact model. Exp Neurol 215(1):69-76. [PubMed: 18938161]  [MGI Ref ID J:144544]

Li Y; Oskouian RJ; Day YJ; Rieger JM; Liu L; Kern JA; Linden J. 2006. Mouse spinal cord compression injury is reduced by either activation of the adenosine A2A receptor on bone marrow-derived cells or deletion of the A2A receptor on non-bone marrow-derived cells. Neuroscience 141(4):2029-39. [PubMed: 16777350]  [MGI Ref ID J:113157]

Liu XL; Zhou R; Pan QQ; Jia XL; Gao WN; Wu J; Lin J; Chen JF. 2010. Genetic inactivation of the adenosine A2A receptor attenuates pathologic but not developmental angiogenesis in the mouse retina. Invest Ophthalmol Vis Sci 51(12):6625-32. [PubMed: 20610844]  [MGI Ref ID J:171393]

Lukashev D; Ohta A; Apasov S; Chen JF; Sitkovsky M. 2004. Cutting edge: Physiologic attenuation of proinflammatory transcription by the Gs protein-coupled A2A adenosine receptor in vivo. J Immunol 173(1):21-4. [PubMed: 15210754]  [MGI Ref ID J:90811]

Matos M; Augusto E; Agostinho P; Cunha RA; Chen JF. 2013. Antagonistic interaction between adenosine A2A receptors and Na+/K+-ATPase-alpha2 controlling glutamate uptake in astrocytes. J Neurosci 33(47):18492-502. [PubMed: 24259572]  [MGI Ref ID J:204161]

Matos M; Augusto E; Santos-Rodrigues AD; Schwarzschild MA; Chen JF; Cunha RA; Agostinho P. 2012. Adenosine A2A receptors modulate glutamate uptake in cultured astrocytes and gliosomes. Glia 60(5):702-16. [PubMed: 22298379]  [MGI Ref ID J:204802]

Mediero A; Frenkel SR; Wilder T; He W; Mazumder A; Cronstein BN. 2012. Adenosine A2A receptor activation prevents wear particle-induced osteolysis. Sci Transl Med 4(135):135ra65. [PubMed: 22623741]  [MGI Ref ID J:186649]

Mediero A; Kara FM; Wilder T; Cronstein BN. 2012. Adenosine A(2A) Receptor Ligation Inhibits Osteoclast Formation. Am J Pathol 180(2):775-86. [PubMed: 22138579]  [MGI Ref ID J:180525]

Mi T; Abbasi S; Zhang H; Uray K; Chunn JL; Xia LW; Molina JG; Weisbrodt NW; Kellems RE; Blackburn MR; Xia Y. 2008. Excess adenosine in murine penile erectile tissues contributes to priapism via A2B adenosine receptor signaling. J Clin Invest 118(4):1491-501. [PubMed: 18340377]  [MGI Ref ID J:135978]

Mills JH; Kim DG; Krenz A; Chen JF; Bynoe MS. 2012. A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis. J Immunol 188(11):5713-22. [PubMed: 22529293]  [MGI Ref ID J:188748]

Mohsenin A; Mi T; Xia Y; Kellems RE; Chen JF; Blackburn MR. 2007. Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice. Am J Physiol Lung Cell Mol Physiol 293(3):L753-61. [PubMed: 17601796]  [MGI Ref ID J:128038]

Naganuma M; Wiznerowicz EB; Lappas CM; Linden J; Worthington MT; Ernst PB. 2006. Cutting edge: Critical role for A2A adenosine receptors in the T cell-mediated regulation of colitis. J Immunol 177(5):2765-9. [PubMed: 16920910]  [MGI Ref ID J:139531]

Nowak M; Lynch L; Yue S; Ohta A; Sitkovsky M; Balk SP; Exley MA. 2009. The A2aR adenosine receptor controls cytokine production in iNKT cells. Eur J Immunol 40(3):682-687. [PubMed: 20039304]  [MGI Ref ID J:157769]

Ohta A; Gorelik E; Prasad SJ; Ronchese F; Lukashev D; Wong MK; Huang X; Caldwell S; Liu K; Smith P; Chen JF; Jackson EK; Apasov S; Abrams S; Sitkovsky M. 2006. A2A adenosine receptor protects tumors from antitumor T cells. Proc Natl Acad Sci U S A 103(35):13132-7. [PubMed: 16916931]  [MGI Ref ID J:112897]

Ohta A; Lukashev D; Jackson EK; Fredholm BB; Sitkovsky M. 2007. 1,3,7-trimethylxanthine (caffeine) may exacerbate acute inflammatory liver injury by weakening the physiological immunosuppressive mechanism. J Immunol 179(11):7431-8. [PubMed: 18025187]  [MGI Ref ID J:154815]

Ohta A; Madasu M; Subramanian M; Kini R; Jones G; Chouker A; Ohta A; Sitkovsky M. 2014. Hypoxia-induced and A2A adenosine receptor-independent T-cell suppression is short lived and easily reversible. Int Immunol 26(2):83-91. [PubMed: 24150242]  [MGI Ref ID J:207022]

Ohta A; Sitkovsky M. 2001. Role of G-protein-coupled adenosine receptors in downregulation of inflammation and protection from tissue damage. Nature 414(6866):916-20. [PubMed: 11780065]  [MGI Ref ID J:111970]

Peng Z; Borea PA; Wilder T; Yee H; Chiriboga L; Blackburn MR; Azzena G; Resta G; Cronstein BN. 2009. Adenosine signaling contributes to ethanol-induced fatty liver in mice. J Clin Invest 119(3):582-94. [PubMed: 19221436]  [MGI Ref ID J:146795]

Reutershan J; Cagnina RE; Chang D; Linden J; Ley K. 2007. Therapeutic anti-inflammatory effects of myeloid cell adenosine receptor A2a stimulation in lipopolysaccharide-induced lung injury. J Immunol 179(2):1254-63. [PubMed: 17617618]  [MGI Ref ID J:149341]

Rork TH; Wallace KL; Kennedy DP; Marshall MA; Lankford AR; Linden J. 2008. Adenosine A2A receptor activation reduces infarct size in the isolated, perfused mouse heart by inhibiting resident cardiac mast cell degranulation. Am J Physiol Heart Circ Physiol 295(5):H1825-33. [PubMed: 18757481]  [MGI Ref ID J:142451]

Roseti C; Martinello K; Fucile S; Piccari V; Mascia A; Di Gennaro G; Quarato PP; Manfredi M; Esposito V; Cantore G; Arcella A; Simonato M; Fredholm BB; Limatola C; Miledi R; Eusebi F. 2008. Adenosine receptor antagonists alter the stability of human epileptic GABAA receptors. Proc Natl Acad Sci U S A 105(39):15118-23. [PubMed: 18809912]  [MGI Ref ID J:143095]

Ryzhov S; Novitskiy SV; Goldstein AE; Biktasova A; Blackburn MR; Biaggioni I; Dikov MM; Feoktistov I. 2011. Adenosinergic regulation of the expansion and immunosuppressive activity of CD11b+Gr1+ cells. J Immunol 187(11):6120-9. [PubMed: 22039302]  [MGI Ref ID J:179751]

Scheibner KA; Boodoo S; Collins S; Black KE; Chan-Li Y; Zarek P; Powell JD; Horton MR. 2009. The adenosine a2a receptor inhibits matrix-induced inflammation in a novel fashion. Am J Respir Cell Mol Biol 40(3):251-9. [PubMed: 18703794]  [MGI Ref ID J:157434]

Sturgess JE; Ting-A-Kee RA; Podbielski D; Sellings LH; Chen JF; van der Kooy D. 2010. Adenosine A1 and A2A receptors are not upstream of caffeine's dopamine D2 receptor-dependent aversive effects and dopamine-independent rewarding effects. Eur J Neurosci 32(1):143-54. [PubMed: 20576036]  [MGI Ref ID J:171784]

Subramanian M; Kini R; Madasu M; Ohta A; Nowak M; Exley M; Sitkovsky M; Ohta A. 2014. Extracellular adenosine controls NKT-cell-dependent hepatitis induction. Eur J Immunol 44(4):1119-29. [PubMed: 24448964]  [MGI Ref ID J:209352]

Tebano MT; Martire A; Potenza RL; Gro C; Pepponi R; Armida M; Domenici MR; Schwarzschild MA; Chen JF; Popoli P. 2008. Adenosine A(2A) receptors are required for normal BDNF levels and BDNF-induced potentiation of synaptic transmission in the mouse hippocampus. J Neurochem 104(1):279-86. [PubMed: 18005343]  [MGI Ref ID J:141449]

Tikh EI; Fenton RA; Dobson JG Jr. 2006. Contractile effects of adenosine A1 and A2A receptors in isolated murine hearts. Am J Physiol Heart Circ Physiol 290(1):H348-56. [PubMed: 16143649]  [MGI Ref ID J:104792]

Tsukamoto H; Chernogorova P; Ayata K; Gerlach UV; Rughani A; Ritchey JW; Ganesan J; Follo M; Zeiser R; Thompson LF; Idzko M. 2012. Deficiency of CD73/ecto-5'-nucleotidase in mice enhances acute graft-versus-host disease. Blood 119(19):4554-64. [PubMed: 22262774]  [MGI Ref ID J:185014]

Wallace KL; Linden J. 2010. Adenosine A2A receptors induced on iNKT and NK cells reduce pulmonary inflammation and injury in mice with sickle cell disease. Blood 116(23):5010-20. [PubMed: 20798237]  [MGI Ref ID J:167256]

Wang H; Zhang W; Tang R; Zhu C; Bucher C; Blazar BR; Geng JG; Zhang C; Linden J; Wu C; Huo Y. 2010. Adenosine receptor A2A deficiency in leukocytes increases arterial neointima formation in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol 30(5):915-22. [PubMed: 20167656]  [MGI Ref ID J:175821]

Wang H; Zhang W; Zhu C; Bucher C; Blazar BR; Zhang C; Chen JF; Linden J; Wu C; Huo Y. 2009. Inactivation of the adenosine A2A receptor protects apolipoprotein E-deficient mice from atherosclerosis. Arterioscler Thromb Vasc Biol 29(7):1046-52. [PubMed: 19407243]  [MGI Ref ID J:167816]

Wen J; Jiang X; Dai Y; Zhang Y; Tang Y; Sun H; Mi T; Phatarpekar PV; Kellems RE; Blackburn MR; Xia Y. 2010. Increased adenosine contributes to penile fibrosis, a dangerous feature of priapism, via A2B adenosine receptor signaling. FASEB J 24(3):740-9. [PubMed: 19858092]  [MGI Ref ID J:158034]

Wilson JM; Kurtz CC; Black SG; Ross WG; Alam MS; Linden J; Ernst PB. 2011. The A2B adenosine receptor promotes Th17 differentiation via stimulation of dendritic cell IL-6. J Immunol 186(12):6746-52. [PubMed: 21593380]  [MGI Ref ID J:175473]

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Yao SQ; Li ZZ; Huang QY; Li F; Wang ZW; Augusto E; He JC; Wang XT; Chen JF; Zheng RY. 2012. Genetic inactivation of the adenosine A(2A) receptor exacerbates brain damage in mice with experimental autoimmune encephalomyelitis. J Neurochem 123(1):100-12. [PubMed: 22639925]  [MGI Ref ID J:190427]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   27-JUL-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Adora2atm1Jfc  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Adora2atm1Jfc x Homozygous for Adora2atm1Jfc  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Adora2atm1Jfc  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Adora2atm1Jfc x Homozygous for Adora2atm1Jfc  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice
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JAX® Services
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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