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| These human Thy-1-COX-2 transgenic mice express human COX-2 (PTGS2 or PGE2) primarily in neurons of the amygdala, striatum, cerebral cortex, and hippocampus as directed by the murine Thy1.2 expression cassette. These overexpressing COX-2 transgenic mice exhibit PGE2 levels that are ~25-40-fold greater than non-transgenic controls. | ||||||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6J-Tg(Thy1-PTGS2)303Kand/J (Changed: 22-SEP-11 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Katrin I Andreasson, Stanford University Description
Mice hemizygous for the human Thy-1-COX-2 transgene (hCOX-2 transgene) are viable and fertile, with expression of human COX-2 (PTGS2 or PGE2) directed primarily to neurons of the amygdala, striatum, cerebral cortex, and hippocampus by the murine Thy1.2 expression cassette. These overexpressing C57BL/6J COX-2 transgenic line 303 mice have PGE2 levels that are ~25-40-fold greater than non-transgenic controls (compared to ~10-12-fold overexpression in line 300; see Stock No. 010800). At approximately 12 months of age, COX-2 transgenic mice develop an age-dependent deficit in spatial memory. Around 20 months of age, a less pronounced, but significant deterioration in performance of non-spatial memory tasks develops. Further progressive memory impairments are observed over time. These cognitive deficits are associated with parallel age-dependent increases in cortical neuronal apoptosis and glial activation. Transgenic mice exhibit enhanced hippocampal longterm synaptic plasticity. Chronic overexpression of neuronal COX-2 enzymatic activity leads to long-term cellular changes that result in diminished neuron viability in transient focal ischemia/ischemic injury challenge. Elevated expression of COX-2 leads to increased oxidative degradation of endocannabinoids (eCBs) resulting in abolished depolarization-induced suppression of inhibition (DSI; or eCB-induced suppression of GABAergic synaptic transmission). These human Thy-1-COX-2 transgenic mice may be useful to model chronic elevations in COX-2 enzymatic activity associated with neurodegenerative diseases and aging, oxidative metabolism of endocannabinoids in modulation of synaptic transmission and plasticity, and ischemia and postischemic inflammatory reaction.Development
The human Thy-1-COX-2 transgene (hCOX-2 transgene) was designed with the entire 1.8 kb human COX-2 (PTGS2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) or PGE2) open reading frame sequence inserted into the 8.2 kb Thy-1 expression cassette (in the second exon of the "murine Thy-1.2 gene"). This transgene was injected into the male pronucleus of C57BL/6J cells. Transgenic founders were bred with C57BL/6J and mice from founder line 303 were found to have an approximately 25-40-fold increase in PGE2 expression. Transgenic mice were bred with C57BL/6NCrl mice for approximately five years prior to sending to The Jackson Laboratory Repository. Upon arrival, sperm from transgenic mice was cryopreserved. When rederiving the live colony, transgenic sperm will be used to fertilize oocytes from C57BL/6NJ females (Stock No. 005304).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (107 strains)
010800 C57BL/6-Tg(Thy1-PTGS2)300Kand/J
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Apoptosis Research
Endogenous Regulators
Extracellular Modulators
Immunology, Inflammation and Autoimmunity Research
Inflammation
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Channel and Transporter Defects
Cortical Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects
Research Tools
Apoptosis Research
Immunology and Inflammation Research
Neurobiology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-PTGS2)303Kand | ||
|---|---|---|---|
| Allele Name | transgene insertion 303, Katrin Andreasson | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Thy-1-COX-2 tg line 303; hCOX-2 tg; | ||
| Mutation Made By | Katrin Andreasson, Stanford University | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | PTGS2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), human | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The transgene construct contains the entire 1.8 kb human COX-2 (PTGS2; prostaglandin-endoperoxide synthase 2 or PGE2) open reading frame sequence inserted into the 8.2 kb Thy-1 expression cassette. This construct was injected into the male pronuclei of C57BL/6J cells. No founder lines were provided in the original reference but lines 300, 303, and 316 were characterized in subsequent publications. Mice from founder line 303 were found to have an approximately 25-40-fold increase in PGE2 expression. [MGI Ref ID J:72034] | ||
References
References provided by MGI
Selected Reference(s)
Andreasson KI; Savonenko A; Vidensky S; Goellner JJ; Zhang Y; Shaffer A; Kaufmann WE; Worley PF; Isakson P; Markowska AL. 2001. Age-dependent cognitive deficits and neuronal apoptosis in cyclooxygenase-2 transgenic mice. J Neurosci 21(20):8198-209. [PubMed: 11588192] [MGI Ref ID J:72034]
Boutaud O; Andreasson KI; Zagol-Ikapitte I; Oates JA. 2005. Cyclooxygenase-dependent lipid-modification of brain proteins. Brain Pathol 15(2):139-42. [PubMed: 15912886] [MGI Ref ID J:156165]
Dore S; Otsuka T; Mito T; Sugo N; Hand T; Wu L; Hurn PD; Traystman RJ; Andreasson K. 2003. Neuronal overexpression of cyclooxygenase-2 increases cerebral infarction. Ann Neurol 54(2):155-62. [PubMed: 12891667] [MGI Ref ID J:156166]
Melnikova T; Savonenko A; Wang Q; Liang X; Hand T; Wu L; Kaufmann WE; Vehmas A; Andreasson KI. 2006. Cycloxygenase-2 activity promotes cognitive deficits but not increased amyloid burden in a model of Alzheimer's disease in a sex-dimorphic pattern. Neuroscience 141(3):1149-62. [PubMed: 16753269] [MGI Ref ID J:111748]
Vidensky S; Zhang Y; hand T; Goellner J; Shaffer A; Isakson P; Andreasson K. 2003. Neuronal overexpression of COX-2 results in dominant production of PGE2 and altered fever response. Neuromolecular Med 3(1):15-28. [PubMed: 12665673] [MGI Ref ID J:156168]
Yang H; Zhang J; Andreasson K; Chen C. 2008. COX-2 oxidative metabolism of endocannabinoids augments hippocampal synaptic plasticity. Mol Cell Neurosci 37(4):682-95. [PubMed: 18295507] [MGI Ref ID J:135668]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6NJ inbred mice (Stock No. 005304).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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