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| Mouse strains created by the Pleiades Promoter Project (Dr. Elizabeth M. Simpson, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Canada) are now available through the Mutant Mouse Regional Resource Center (MMRRC) at The Jackson Laboratory. For the Ple49-lacZ mice, please see MMRRC #032936 at the MMRRC website. | ||||||||||||||||||||||
| These mice have the Ple49-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human DBH gene direct expression of β-galactosidase. | ||||||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
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Description
Strain Information
Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N3pN1
Generation DefinitionsDonating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple49-lacZ;mEMS3682 mice have the Ple49-lacZ transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene directing expression of β-galactosidase (lacZ). These Ple49-lacZ;mEMS3682 mice may be useful in studying DBH-expressing cells in the brain and diseases affecting the brain.For more information, see The Pleiades Promoter Project website (Ple49 Promoter (pEMS1520)). The donating investigator reports:
Ple49-lacZ;mEMS3682 mice show an enriched lacZ expression in noradrenergic cells in the locus coeruleus, with strong lacZ expression in the adrenal gland where it is coexpressed with tyrosine hydroxylase.Of note, Ple49-EGFP mice have expression of EGFP directed by the same minipromoter. These Ple49-EGFP are described by the donating investigator as:
MiniPromoter Ple49 was assayed for EGFP from pEMS1306 backbone. No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).
Development
The Ple49-lacZ transgene (pEMS1520) was designed with the 756 bp Ple49 minipromoter (DBH-B; derived from a subsection of the promoter and an upstream putative regulatory element of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene) upstream of a frt-flanked β-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1202 embryonic stem cells (generated from F1 generation male pups [heterozygous for the Hprtb-m3 allele and wildtype at the Gt(ROSA)26Sor locus] born from C57BL/6-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] and 129S-Gt(ROSA)26Sortm1Sor heterozygous males [see Stock No. 002171 and 003310, respectively]). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS3682) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. These Ple49-lacZ;mEMS3682 mutant mice were bred with C57BL/6J wildtype mice for three generations. Black mice still segregating for the Tyrc-2J mutation were sent to The Jackson Laboratory. Upon arrival, Ple49-lacZ;mEMS3682 mutant males were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice (Stock No. 000664) to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony. The rederived mice may still be segregating for the Tyrc-2J mutation.For more information, see The Pleiades Promoter Project website (Ple49 Promoter (pEMS1520)).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Pleiades Promoter Project GFP strains
View Pleiades Promoter Project GFP strains (25 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Hprttm44(Ple49-lacZ)Ems/Y
B6.129P2(Cg)-Hprttm44(Ple49-lacZ)Ems/Mmjax
- normal phenotype
- no abnormal phenotype detected
- heterozygous females and hemizygous males are viable and fertile (MGI Ref ID J:145689)
This mouse can be used to support research in many areas including:
Neurobiology Research
lacZ expression in neural tissue
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Hprttm44(Ple49-lacZ)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 44, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | DBH-B-lacZ; Hprttm44(mEMS3682)Ems; Ple49; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | (B6.129P2-Hprt | ||
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; Hgprtase; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1; | ||
| Associated Marker Note | Expressed-count: 1Ex1-Source: E. coliEx1-Gene: lacZDriver-count: 1Dr1-Source: HumanDr1-Gene: 1621 | ||
| General Note | Germ line transmission of mutant cell line mEMS3682 has been established. | ||
| Molecular Note |
The Ple49-lacZ transgene (pEMS1520) was designed with the 756 bp Ple49 minipromoter (DBH-B; derived from a subsection of the promoter and an upstream putative regulatory element of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human dopamine beta-hydroxylase (DBH) gene directs expression of beta galactosidase (lacZ) to the noradrenergic cells in the locus coeruleus, with strong expression inthe adrenal gland where it is coexpressed with tyrosine hydroxylase. [MGI Ref ID J:144244] [MGI Ref ID J:164356] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Robotic STD
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Hprttm44(Ple49-lacZ)Ems relatedPortales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF; Bonaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Simpson EM:. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 107(38):16589-94. [PubMed: 20807748] [MGI Ref ID J:164356]
The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699] [MGI Ref ID J:144244]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664).
Purchasing information
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
General Supply Notes
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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