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| When these transgenic mice are crossed with a tissue or cell-specific cre strain to remove a floxed stop cassette, tetracycline-controlled transactivator (tTA) is expressed in hippocampal CA3 and the dentate gyrus under the control of the calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. When further mated to a transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names C57BL/6-Tg(Camk2a-tTA)8082Mmay/J (Changed: 25-JAN-10 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 15-JUN-10 Species laboratory mouse Generation F?+F3 (30-JUN-11)
Generation DefinitionsDonating Investigator Susumu Tonegawa, Massachusetts Institute of Technology Description
When these transgenic mice are crossed with a tissue or cell-specific cre strain to remove a floxed stop cassette, tetracycline-controlled transactivator (tTA) is expressed in hippocampal CA3 and the dentate gyrus under the control of the calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter.When further mated to a transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. Expression in tTA/tetO animals can be reversibly inhibited by the presence of doxycycline or induced by withdrawal of the tetracycline analog.
Development
A Camk2a (αCamKII) promoter followed by a loxP-flanked STOP cassette and tetracycline-controlled transactivator (tTA) were introduced to C57BL/6 embryos. This strain was created and maintained on a C57BL/6 background (see SNP note below) by the donating laboratory.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Camk2a
View Strains carrying other alleles of Camk2a (17 strains)
Additional Web Information
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Neurobiology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Camk2a-tTA)1Stl | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Susumu Tonegawa | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg2; alphaCamKII-loxP-STOP-loxP-tTA; | ||
| Mutation Made By | Susumu Tonegawa, Massachusetts Institute of Technology | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory | ||
| Molecular Note | The transgene construct contained the tetracycline transactivator (tTA) coding sequence downstream of a loxP-STOP-loxP fragement. This was inserted downstream of the alpha-CamKII (Camk2a) promoter in the final construct. Pronuclei of fertilized C57BL/6 eggs were injected with the purified linearized construct. A founder line 1 was established by backcrossing the founder with C57BL/6 mice. [MGI Ref ID J:131852] | ||
Genotyping
Genotyping Information
Genotyping Protocols
010712 - C57BL/6-Tg(Camk2a-tTA)1Stl/J, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Nakashiba T; Young JZ; McHugh TJ; Buhl DL; Tonegawa S. 2008. Transgenic inhibition of synaptic transmission reveals role of CA3 output in hippocampal learning. Science 319(5867):1260-4. [PubMed: 18218862] [MGI Ref ID J:131852]
Additional References
Tg(Camk2a-tTA)1Stl relatedTorborg CL; Nakashiba T; Tonegawa S; McBain CJ. 2010. Control of CA3 output by feedforward inhibition despite developmental changes in the excitation-inhibition balance. J Neurosci 30(46):15628-37. [PubMed: 21084618] [MGI Ref ID J:166441]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes or homozygotes may be bred. Mating System Noncarrier x Hemizygote (Female x Male) 15-JUN-10 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Camk2a-tTA)1Stl
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Camk2a-tTA)1Stl x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Camk2a-tTA)1Stl Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Camk2a-tTA)1Stl
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Camk2a-tTA)1Stl x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Camk2a-tTA)1Stl Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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