Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator Chen Dong,   MD Anderson Cancer Center

Description
Mice that are hemizygous for this transgene are viable, fertile and normal in size. By three months of age, hemizygotes exhibit lung inflammation characterized by hypertrophic lung epithelium in the bronchus and bronchiole as well as thickening of the alveolar walls. After 3 months, large eosinophilic macrophages are observed in lung parenchyma, followed by infiltration of CD4+ lymphocytes beginning at 5 months. Older mice exhibit thin crystals in the bronchioles, mucus production, collagen accumulation in bronchioles and increased chemokine expression. This mutant mouse strain may be useful in studies of airway inflammation and T-cell mediated inflammatory diseases.

Development
A transgenic construct containing a mouse uteroglobin (Scgb1a1) promoter, mouse interleukin 17 (Il17) cDNA and an IRES-GFP cassette was injected into C57BL/6 fertilized embryos. Founder animals were bred to C57BL/6 mice.

SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (4 of 5 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Il17a

018472	C57BL/6-Il17atm1Bcgen/J
016879	STOCK Il17atm1.1(icre)Stck/J

View Strains carrying other alleles of Il17a     (2 strains)

Strains carrying other alleles of Scgb1a1

016225	B6N.129S6(Cg)-Scgb1a1tm1(cre/ERT)Blh/J
012890	C57BL/6-Tg(Scgb1a1-Il17f,GFP)1Cdon/J

View Strains carrying other alleles of Scgb1a1     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tg(Scgb1a1-Il17a)3Cdon/0

        C57BL/6-Tg(Scgb1a1-Il17a)3Cdon

• respiratory system phenotype
• abnormal bronchiole morphology
  • in 5 month-old mice, bronchioles contain long thin neendle-like crystals   (MGI Ref ID J:112600)
• abnormal lung epithelium morphology
  • at 3 months of age, epithelium is hypertrophic in bronchus and bronchioles   (MGI Ref ID J:112600)
  • mucous production by airway epithelium is increased compared to controls   (MGI Ref ID J:112600)
  • subepithelium of bronchioles has substantial deposits of collagen in contrast to controls   (MGI Ref ID J:112600)
• lung inflammation
  • after 3 months of age, parenchyma shows large eosinophilic macrophage infiltration   (MGI Ref ID J:112600)
  • between 5 and 10 months, mice have focal accumulation and infiltration of mononuclear cells including nodular collections of mononuclear CD4 lymphocytes   (MGI Ref ID J:112600)
• thick pulmonary interalveolar septum
  • alveolar walls are thickened compared to controls   (MGI Ref ID J:112600)
• immune system phenotype
• abnormal chemokine level
  • chemokines including CCL7, CCL10, CCL20, CCL11 and CXCL1 show increased expression in lungs of transgenics; TIMP-1 and MMP-9 levels in lungs are elevated   (MGI Ref ID J:112600)
• lung inflammation
  • after 3 months of age, parenchyma shows large eosinophilic macrophage infiltration   (MGI Ref ID J:112600)
  • between 5 and 10 months, mice have focal accumulation and infiltration of mononuclear cells including nodular collections of mononuclear CD4 lymphocytes   (MGI Ref ID J:112600)
• homeostasis/metabolism phenotype
• abnormal chemokine level
  • chemokines including CCL7, CCL10, CCL20, CCL11 and CXCL1 show increased expression in lungs of transgenics; TIMP-1 and MMP-9 levels in lungs are elevated   (MGI Ref ID J:112600)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Inflammation

Internal/Organ Research
Lung Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(Scgb1a1-Il17a)3Cdon
Allele Name		transgene insertion 3, Chen Dong
Allele Type		Transgenic (random, expressed)
Common Name(s)		Cc10-Il17a;
Mutation Made By		Chen Dong,   MD Anderson Cancer Center
Strain of Origin		C57BL/6
Expressed Gene		Il17a, interleukin 17A, mouse, laboratory
Promoter		Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), mouse, laboratory
Molecular Note		A transgenic construct containing a mouse uteroglobin Scgb1a1 promoter, mouse interleukin 17 (Il17a) cDNA and an IRES-GFP cassette was injected into C57BL/6 fertilized embryos. Founder animals from line 3 were bred to C57BL/6 mice. [MGI Ref ID J:112600]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Park H; Li Z; Yang XO; Chang SH; Nurieva R; Wang YH; Wang Y; Hood L; Zhu Z; Tian Q; Dong C. 2005. A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol 6(11):1133-41. [PubMed: 16200068]  [MGI Ref ID J:112600]

Additional References

Tg(Scgb1a1-Il17a)3Cdon related

Shan M; Yuan X; Song LZ; Roberts L; Zarinkamar N; Seryshev A; Zhang Y; Hilsenbeck S; Chang SH; Dong C; Corry DB; Kheradmand F. 2012. Cigarette smoke induction of osteopontin (SPP1) mediates T(H)17 inflammation in human and experimental emphysema. Sci Transl Med 4(117):117ra9. [PubMed: 22261033]  [MGI Ref ID J:183702]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not tried to maintain this strain as a homozygous colony.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.