Strain Name:

B6;129P2-Prdm9tm1Ymat/J

Stock Number:

010719

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Availability:

Cryopreserved - Ready for recovery

Mice homozygous for this knockout allele exhibit germ cell defects resulting in sterility.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating Investigator Yasuhisa Matsui,   Tohoku University

Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are viable but sterile. No gene product (mRNA or protein) is detected by RT-PCR analysis of testes from homozygotes. Male homozygotes display decreased testis weight and incomplete spermatogenesis (arrest at the pachytene stage). Female homozygotes exhibit reduced germ cell number in neonatal ovaries and no forming follicles. Homozygotes have abnormal homologous chromosome pairing, impaired double strand break repair and defective sex body (XY body) formation. This mutant mouse strain may be useful in studies of meiotic arrest, infertility, and double-stranded break repair.

Development
A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette was used to disrupt part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for an unknown number of generations.

Control Information

  Control
   Wild-type siblings from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Prdm9tm1Ymat/Prdm9tm1Ymat

        involves: 129P2/OlaHsd * C57BL/6
  • reproductive system phenotype
  • abnormal gametogenesis   (MGI Ref ID J:102875)
    • abnormal chromosome pairing during meiosis
      • deficient pairing of homologous chromosomes   (MGI Ref ID J:102875)
    • abnormal female meiosis
      • meitotic arrest with impaired homologous chromosome pairing and sex body formation   (MGI Ref ID J:102875)
      • pachytene chromosomes are frequently branched and connected to other chromosomes   (MGI Ref ID J:102875)
    • arrest of male meiosis
      • meitotic arrest with impaired homologous chromosome pairing and sex body formation   (MGI Ref ID J:102875)
      • pachytene chromosomes are frequently branched and connected to other chromosomes   (MGI Ref ID J:102875)
    • azoospermia   (MGI Ref ID J:102875)
    • decreased oocyte number
      • from E17.5 the number of germ cells is decreased with few present in neonates   (MGI Ref ID J:102875)
  • abnormal ovarian folliculogenesis
    • at 5 weeks of age no growing follicles at any stage of development are seen   (MGI Ref ID J:102875)
  • decreased testis weight
    • at 8 weeks of age, testis weigh 75% less than in wild-type mice   (MGI Ref ID J:102875)
  • female infertility   (MGI Ref ID J:102875)
  • male infertility   (MGI Ref ID J:102875)
  • endocrine/exocrine gland phenotype
  • abnormal ovarian folliculogenesis
    • at 5 weeks of age no growing follicles at any stage of development are seen   (MGI Ref ID J:102875)
  • decreased testis weight
    • at 8 weeks of age, testis weigh 75% less than in wild-type mice   (MGI Ref ID J:102875)
  • cellular phenotype
  • abnormal double-strand DNA break repair
    • germ cells show impaired double strand break repair compared to wild-type   (MGI Ref ID J:102875)
  • homeostasis/metabolism phenotype
  • abnormal double-strand DNA break repair
    • germ cells show impaired double strand break repair compared to wild-type   (MGI Ref ID J:102875)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other
      DNA Repair

Cell Biology Research
DNA Damage Response

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects

Research Tools
Reproductive Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Prdm9tm1Ymat
Allele Name targeted mutation 1, Yasuhisa Matsui
Allele Type Targeted (Null/Knockout)
Common Name(s) Meisetz-;
Mutation Made By Yasuhisa Matsui,   Tohoku University
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Prdm9, PR domain containing 9
Chromosome 17
Gene Common Name(s) BC012016; Dsbc1; G1-419-29; MEISETZ; MGC:21428; MSBP3; PFM6; PRMD9; Prdm7; Rcr1; ZNF899; cDNA sequence BC012016; double strand break control 1; recombination regulator 1; repro7; reproductive mutant 7, JAX Reproductive Mutagenesis Program;
Molecular Note A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette disrupted part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. RT-PCR analysis of testes from homozygous mutant mice did not detect transcript. [MGI Ref ID J:102875]

Genotyping

Genotyping Information

Genotyping Protocols

Prdm9tm1Ymat, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hayashi K; Yoshida K; Matsui Y. 2005. A histone H3 methyltransferase controls epigenetic events required for meiotic prophase. Nature 438(7066):374-8. [PubMed: 16292313]  [MGI Ref ID J:102875]

Additional References

Prdm9tm1Ymat related

Brick K; Smagulova F; Khil P; Camerini-Otero RD; Petukhova GV. 2012. Genetic recombination is directed away from functional genomic elements in mice. Nature 485(7400):642-5. [PubMed: 22660327]  [MGI Ref ID J:186641]

Flachs P; Bhattacharyya T; Mihola O; Pialek J; Forejt J; Trachtulec Z. 2014. Prdm9 incompatibility controls oligospermia and delayed fertility but no selfish transmission in mouse intersubspecific hybrids. PLoS One 9(4):e95806. [PubMed: 24756080]  [MGI Ref ID J:215170]

Flachs P; Mihola O; Simecek P; Gregorova S; Schimenti JC; Matsui Y; Baudat F; de Massy B; Pialek J; Forejt J; Trachtulec Z. 2012. Interallelic and intergenic incompatibilities of the Prdm9 (Hst1) gene in mouse hybrid sterility. PLoS Genet 8(11):e1003044. [PubMed: 23133405]  [MGI Ref ID J:194116]

Mihola O; Trachtulec Z; Vlcek C; Schimenti JC; Forejt J. 2009. A mouse speciation gene encodes a meiotic histone h3 methyltransferase. Science 323(5912):373-5. [PubMed: 19074312]  [MGI Ref ID J:143556]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are viable but sterile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type siblings from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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