Strain Name:

STOCK Tgfb1tm2.1Doe/J

Stock Number:

010721

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Availability:

Repository- Live

These Tgfb1flox-ex 6 mutant mice harbor loxP sites flanking exon 6 of the Tgfb1 (transforming growth factor, beta 1) gene and may be useful in generating conditional mutations for studying the role of TGFβ1 signaling in cancer, wound healing, tissue fibrosis, and muscular, neurological, ophthalmic, cardiovascular and immunological disorders, as well as other human diseases including Marfan syndrome and Camurati-Engelmann disease.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   21-APR-11
Specieslaboratory mouse
GenerationF?+N1F3 (09-OCT-13)
Generation Definitions
 
Donating Investigator Thomas Doetschman,   University of Arizona

Description
Mice homozygous for this Tgfb1flox-ex 6 conditional allele are viable and fertile, with loxP sites flanking exon 6 of the Tgfb1 (transforming growth factor, beta 1) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissues while leaving the precursor pro-region and neighboring B9d2 (LOC232987) gene intact.

Development
A targeting vector was designed to place a loxP-flanked PMC1-Neo cassette just upstream of exon 6, and a third loxP site just downstream of exon 6 of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived KG-1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre recombinase-expressing plasmid. The resulting ES cells with the Tgfb1flox-ex 6 genotype (neo selection cassette removed; leaving a single loxP site upstream of exon 6 and a single loxP site downstream of exon 6) were injected into recipient blastocysts. Chimeric males were bred with Black-Swiss females to generate the Tgfb1flox-ex 6 colony. Homozygous mice were bred together for approximately five generations and then bred with Tgfb1- mice (on a mixed CF-1, 129 and BALB/c genetic background; see Stock No. 002098). The Tgfb1flox-ex 6/- mice were bred together for approximately ten generations. Offspring homozygous for the Tgfb1flox-ex 6 allele were sent to The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the Tgfb1flox-ex 6 colony.

In September 2011, the living Tgfb1flox-ex 6 mouse colony at The Jackson Laboratory Repository was found to be homozygous for the Tgfb1flox-ex 6 allele and to also be segregating for the Cre recombinase gene. The Cre recombinase expression was unlikely to be in germline (as the colony was homozygous for the Tgfb1flox-ex 6 allele using an assay that would not produce a PCR product for a deletion allele). Whether or not the source of Cre recombinase was from previously mating Tgfb1flox-ex 6 mice with LckCre mice is not known. Mice double confirmed to be Cre-negative were selected and bred to C57BL/6J inbred mice to repopulate the colony. By May 2012, the living Tgfb1flox-ex 6 mouse colony at The Jackson Laboratory Repository was homozygous for the Tgfb1flox-ex 6 mutation and without Cre recombinase.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tgfb1
024931   129-Tgfb1tm1(Tgfb3)Kul/J
002220   B6.129S2-Tgfb1tm1Doe/J
002098   STOCK Tgfb1tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
View Strains carrying other alleles of Tgfb1     (4 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Camurati-Engelmann Disease; CAEND   (TGFB1)
Cystic Fibrosis; CF   (TGFB1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tgfb1tm1Doe/Tgfb1tm2.1Doe Tg(Lck-cre)548Jxm/?

        involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss * BALB/c * C57BL/6 * CBA   (conditional)
  • immune system phenotype
  • lung inflammation
    • inflammation is observed in the lungs at 5 months of age   (MGI Ref ID J:150024)
  • salivary gland inflammation
    • salivary gland inflammation is observed in mice at 5 months of age   (MGI Ref ID J:150024)
  • respiratory system phenotype
  • lung inflammation
    • inflammation is observed in the lungs at 5 months of age   (MGI Ref ID J:150024)
  • digestive/alimentary phenotype
  • salivary gland inflammation
    • salivary gland inflammation is observed in mice at 5 months of age   (MGI Ref ID J:150024)
  • endocrine/exocrine gland phenotype
  • salivary gland inflammation
    • salivary gland inflammation is observed in mice at 5 months of age   (MGI Ref ID J:150024)

Tg(Lck-cre)548Jxm/? Tgfb1tm2.1Doe/Tgfb1tm2.1Doe

        involves: 129S6/SvEvTac * Black Swiss * C57BL/6 * CBA   (conditional)
  • growth/size/body phenotype
  • weight loss
    • there is more than a 10% reduction in mean weight of mice compared to controls at 5 months of age   (MGI Ref ID J:150024)
  • digestive/alimentary phenotype
  • salivary gland inflammation
    • modest salivary gland inflammation is observed in mice at 5 months of age   (MGI Ref ID J:150024)
  • immune system phenotype
  • abnormal T cell activation
    • there is a higher percentage of T cells expressing activation markers   (MGI Ref ID J:150024)
  • abnormal T cell subpopulation ratio
    • there are slightly more CD8+ T cells than CD4+ T cells among splenocytes   (MGI Ref ID J:150024)
  • abnormal immune system organ morphology   (MGI Ref ID J:150024)
    • decreased spleen weight
      • spleen weight is reduced by about a third at 2 months of age   (MGI Ref ID J:150024)
    • decreased thymus weight
      • thymus weight at 5 months of age is reduced by more than a third   (MGI Ref ID J:150024)
    • spleen hypoplasia
      • spleen cellularity is about half that of controls at 5 months of age   (MGI Ref ID J:150024)
    • thymus hypoplasia
      • thymus cellularity is reduced by more than two-thirds at 1 month of age   (MGI Ref ID J:150024)
  • increased inflammatory response   (MGI Ref ID J:150024)
    • lung inflammation
      • modest inflammation is observed in the lungs at 5 months of age   (MGI Ref ID J:150024)
    • salivary gland inflammation
      • modest salivary gland inflammation is observed in mice at 5 months of age   (MGI Ref ID J:150024)
  • increased interferon-gamma secretion
    • interferon-gamma secretion is enhanced in mutant T cells when activated with various stimuli   (MGI Ref ID J:150024)
  • increased interleukin-2 secretion
    • interleukin-2 secretion is enhanced in mutant T cells when activated with various stimuli   (MGI Ref ID J:150024)
  • respiratory system phenotype
  • lung inflammation
    • modest inflammation is observed in the lungs at 5 months of age   (MGI Ref ID J:150024)
  • endocrine/exocrine gland phenotype
  • decreased thymus weight
    • thymus weight at 5 months of age is reduced by more than a third   (MGI Ref ID J:150024)
  • salivary gland inflammation
    • modest salivary gland inflammation is observed in mice at 5 months of age   (MGI Ref ID J:150024)
  • thymus hypoplasia
    • thymus cellularity is reduced by more than two-thirds at 1 month of age   (MGI Ref ID J:150024)
  • hematopoietic system phenotype
  • abnormal T cell activation
    • there is a higher percentage of T cells expressing activation markers   (MGI Ref ID J:150024)
  • abnormal T cell subpopulation ratio
    • there are slightly more CD8+ T cells than CD4+ T cells among splenocytes   (MGI Ref ID J:150024)
  • decreased spleen weight
    • spleen weight is reduced by about a third at 2 months of age   (MGI Ref ID J:150024)
  • decreased thymus weight
    • thymus weight at 5 months of age is reduced by more than a third   (MGI Ref ID J:150024)
  • spleen hypoplasia
    • spleen cellularity is about half that of controls at 5 months of age   (MGI Ref ID J:150024)
  • thymus hypoplasia
    • thymus cellularity is reduced by more than two-thirds at 1 month of age   (MGI Ref ID J:150024)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines

Cell Biology Research
Signal Transduction

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Internal/Organ Research
Wound Healing

Research Tools
Cancer Research
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tgfb1tm2.1Doe
Allele Name targeted mutation 2.1, Thomas Doetschman
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Tgfb1flox ex6;
Mutation Made By Thomas Doetschman,   University of Arizona
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Tgfb1, transforming growth factor, beta 1
Chromosome 7
Gene Common Name(s) CED; DPD1; LAP; TGF-beta 1; TGF-beta1; TGFB; TGFbeta; TGFbeta1; Tgfb; Tgfb-1;
Molecular Note A floxed neomycin cassette was placed upstream of exon 6 with an additional loxP site placed downstream of exon 6. The neomycin cassette was subsequently removed by transient expression of cre recombinase leaving exon 6 floxed. Germline transmission ofthe allele was confirmed by genomic PCR. [MGI Ref ID J:150024]

Genotyping

Genotyping Information

Genotyping Protocols

Tgfb1tm2.1Doe, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tgfb1tm2.1Doe related

Azhar M; Yin M; Bommireddy R; Duffy JJ; Yang J; Pawlowski SA; Boivin GP; Engle SJ; Sanford LP; Grisham C; Singh RR; Babcock GF; Doetschman T. 2009. Generation of mice with a conditional allele for transforming growth factor beta 1 gene. Genesis 47(6):423-31. [PubMed: 19415629]  [MGI Ref ID J:150024]

Meyer A; Wang W; Qu J; Croft L; Degen JL; Coller BS; Ahamed J. 2012. Platelet TGF-beta1 contributions to plasma TGF-beta1, cardiac fibrosis, and systolic dysfunction in a mouse model of pressure overload. Blood 119(4):1064-74. [PubMed: 22134166]  [MGI Ref ID J:181748]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous or homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   21-APR-11
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Tgfb1tm2.1Doe  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Tgfb1tm2.1Doe x Homozygous for Tgfb1tm2.1Doe  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Tgfb1tm2.1Doe  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Tgfb1tm2.1Doe x Homozygous for Tgfb1tm2.1Doe  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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