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| Mouse strains created by the Pleiades Promoter Project (Dr. Elizabeth M. Simpson, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Canada) are now available through the Mutant Mouse Regional Resource Center (MMRRC) at The Jackson Laboratory. For the Ple233-EGFP mice, please see MMRRC #032938 at the MMRRC website. | ||||||||||||||||||||||
| These mice have the Ple233-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human TNNT1 gene direct expression of EGFP. | ||||||||||||||||||||||
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- Health & husbandry
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N3pN1
Generation DefinitionsDonating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple233-EGFP;mEMS3251 mice have the Ple233-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human troponin T type 1 (skeletal, slow) [TNNT1] gene directing expression of an enhanced green fluorescent protein (EGFP). These Ple233-EGFP;mEMS3251 mice may be useful in studying TNNT1-expressing cells in the brain and diseases affecting the brain.The donating investigator reports:
These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).
Development
The Ple233-EGFP transgene (pEMS1437) was designed with the 1216 bp Ple233 minipromoter (TNNT1-C; derived from a subsection of the promoter and a downstream putative regulatory element from the human troponin T type 1 (skeletal, slow) [TNNT1] gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1204 embryonic stem cells (generated from F1 generation male pups born of C57BL/6-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS3251) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. The donating investigator reports that these Ple233-EGFP;mEMS3251 mutant mice were bred with C57BL/6J wild-type mice for at least three generations prior to sending to The Jackson Laboratory. Upon arrival, Ple233-EGFP;mEMS3251 mutant males were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice (Stock No. 000664) to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony. The rederived mice should be screened and selectively bred to be sure they are no longer segregating for the Gt(ROSA)26Sortm1Sor mutation. mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least two generations to establish the colony.For more information, see The Pleiades Promoter Project website (Ple233 Promoter (pEMS1437)).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
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Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Hprttm54(Ple233-EGFP)Ems/Y
B6.129P2(Cg)-Hprttm54(Ple233-EGFP)Ems/Mmjax
- normal phenotype
- no abnormal phenotype detected
- heterozygous females and hemizygous males are viable and fertile (MGI Ref ID J:145689)
This mouse can be used to support research in many areas including:
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Hprttm54(Ple233-EGFP)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 54, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprttm54(mEMS3251)Ems; Ple233; TNNT1-EFGP; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | (B6.129P2-Hprt | ||
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1; | ||
| Associated Marker Note | Expressed-count: 1Ex1-Source: JellyfishEx1-Gene: EGFPDriver-count: 1Dr1-Source: HumanDr1-Gene: 7138 | ||
| General Note | Germ line transmission of mutant cell line mEMS3251 has been established. | ||
| Molecular Note | The Ple233-EGFP transgene (pEMS1437) was designed with the 1216 bp Ple233 minipromoter (TNNT1-C; derived from a subsection of the promoter and a downstream putative regulatory element from the human troponin T type 1 (skeletal, slow) [TNNT1] gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. [MGI Ref ID J:144244] [MGI Ref ID J:164356] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Hprttm54(Ple233-EGFP)Ems relatedPortales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF; Bonaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Simpson EM:. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 107(38):16589-94. [PubMed: 20807748] [MGI Ref ID J:164356]
The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699] [MGI Ref ID J:144244]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664).
Purchasing information
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
General Supply Notes
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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