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| These Dlx5/6-Flpe mice have expression of Myc-tagged FLPe recombinase directed to GABAergic neuron populations in the forebrain. When bred with mice containing an FRT-flanked sequence, FLPe-mediated recombination will result in deletion of the FRT-flanked sequence in forebrain GABAergic populations of the offspring. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(Dlx5/Dlx6-FLPe)39Fsh/J (Changed: 19-FEB-10 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Gord Fishell, NYU School of Medicine Description
Hemizygous Dlx5/6-Flpe mice are viable and fertile, with expression of Myc-tagged FLPe recombinase directed to GABAergic neuron populations in the forebrain by the mouse Dlx5/Dlx6 (id6/id5) intergenic enhancer region and β-globin basal promoter. The donating investigator reports that no abnormal phenotype is observed in hemizygous mice. When bred with mice containing an FRT-flanked sequence, FLPe-mediated recombination will result in deletion of the FRT-flanked sequence in forebrain GABAergic populations of the offspring.Development
The Dlx5/6-Flpe transgene was designed with the intergenic enhancer region between the mouse Dlx5 and Dlx6 (id6/id5) locus together with a β-globin basal promoter, a chimeric intron containing a human β-globin splice donator site and IgHC variable region splice acceptor site, a Flpe site-specific recombinase with Myc tag (FlpeMyc) sequence, and SV40 early mRNA polyadenylation signal cassette. This transgene was microinjected into FVB/N mouse cells. Mice from founder line 39 were mated with outbred Swiss Webster mice for many generations. Dlx5/6-Flpe mice predominantly on an outbred Swiss Webster genetic background (and homozygous for the the retinal degeneration allele Pde6brd1) were sent to The Jackson Laboratory. Upon arrival, sperm from transgenic mice was cryopreserved. Some of the cryopreserved sperm was used to fertilize oocytes from FVB/NJ inbred mice (Stock No. 001800) to establish the Dlx5/6-Flpe colony. When maintained by breeding Dlx5/6-Flpe mice together or to FVB/NJ inbred mice, the Dlx5/6-Flpe mice should also be homozygous for the retinal degeneration allele Pde6brd1.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Dlx5
010705 B6(Cg)-Dlx5tm1(cre/ERT2)Zjh/J View Strains carrying other alleles of Dlx5 (1 strain)
Additional Web Information
FLP-FRT Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
FLP1 relatedResearch Tools
FLP-FRT System
FLP Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: FLP recombinase
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: FLP recombinase
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(mI56i-FLPe)39Fsh | ||
|---|---|---|---|
| Allele Name | transgene insertion 39, Gordon Fishell | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Dlx5/6-FLPe; | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Myc-tagged FLPe recombinase is expressed in GABAergic neuron populations in the forebrain. | ||
| Expressed Gene | FLP1, FLP1 recombinase, Saccharomyces cerevisiae | ||
| FLP1 recombinase is an enzyme derived from Saccharomyces cerevisiae that specifically recognizes a 34 base pair FLP1 recombinase target (FRT) sequence. FLP1 has been shown to effectively mediate the excision of DNA located between similarly oriented FRT sites. After the excision event, DNA ends recombine leaving a single FRT site in place of the intervening sequence. DNA located between oppositely orientated FRT sites is inverted rather than deleted. | |||
| Promoter | Dlx5, distal-less homeobox 5, mouse, laboratory | ||
| Promoter | Dlx6, distal-less homeobox 6, mouse, laboratory | ||
| Driver Note | mI56i | ||
| Molecular Note | The Dlx5/6-FLPe transgene was designed with the intergenic enhancer region between the mouse Dlx5 and Dlx6 (id6/id5, mI56i) locus together with a beta-globin basal promoter, a chimeric intron containing a human _-globin splice donator site and IgHC variable region splice acceptor site, a FLPe site-specific recombinase with Myc tag (FLPeMyc) sequence, and SV40 early mRNA polyadenylation signal cassette. This transgene was microinjected into FVB/N mouse cells. Mice from founder line 39 were mated with outbred Swiss Webster mice for many generations. [MGI Ref ID J:156662] | ||
| Gene Symbol and Name | Tg(mI56i-FLPe)39Fsh, transgene insertion 39, Gordon Fishell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Dlx5/6-FLPe; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(mI56i-FLPe)39Fsh, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Tg(mI56i-FLPe)39Fsh relatedFishell G. 2010. Generation of an FLPe transgenic allele MGI Direct Data Submission :. [MGI Ref ID J:156662]
Imayoshi I; Hirano K; Sakamoto M; Miyoshi G; Imura T; Kitano S; Miyachi H; Kageyama R. 2012. A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination. Neurosci Res 73(1):85-91. [PubMed: 22343123] [MGI Ref ID J:182745]
Jeanneteau F; Deinhardt K; Miyoshi G; Bennett AM; Chao MV. 2010. The MAP kinase phosphatase MKP-1 regulates BDNF-induced axon branching. Nat Neurosci 13(11):1373-9. [PubMed: 20935641] [MGI Ref ID J:166927]
Miyoshi G; Hjerling-Leffler J; Karayannis T; Sousa VH; Butt SJ; Battiste J; Johnson JE; Machold RP; Fishell G. 2010. Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons. J Neurosci 30(5):1582-94. [PubMed: 20130169] [MGI Ref ID J:157935]
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. [PubMed: 21943598] [MGI Ref ID J:177261]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgene carrier mice may be bred to wildtype (noncarrier) mice from the colony.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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