Strain Name:

129S-Per2tm1Drw/J

Stock Number:

010832

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Availability:

Cryopreserved - Ready for recovery

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Mice that are homozygous for this targeted mutation of Per2, period homolog 2 (Drosophila), exhibit a short circadian period initially when housed in constant darkness, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129S4/SvJae-Per2tm1Drw/J    (Changed: 02-NOV-09 )
129S-Per2tm1Drw/J    (Changed: 29-SEP-09 )
Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN1pN1
Generation Definitions
 
Donating Investigator David R. Weaver,   Univ of Massachusetts Medical School

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Abberant gene products (mRNA) are detected by RT-PCR and RACE analysis of brain total RNA. No gene product (protein) is detected in homozygous mutant mice by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. Western blots of liver reveal a deletion mutant protein product is produced. When housed in constant darkness, homozygotes have a short circadian period initially, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.

Development
A targeting vector containing a PGKneo cassette was used to disrupt exon 5 and a portion of exon 6. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to 129S4/SvJae female mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, mutant mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
   009104 129S4/SvJaeJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Per2tm1Drw allele
010492   B6.129-Per2tm1Drw/J
View Strains carrying   Per2tm1Drw     (1 strain)

Strains carrying other alleles of Per2
016176   B6(Cg)-Tg(tetO-Per2)2Jt/J
006852   B6.129S6-Per2tm1Jt/J
003819   B6.Cg-Per2tm1Brd Tyrc-Brd/J
013082   B6.FVB-Tg(Per2-DsRed*T3)12Obr/Mmjax
View Strains carrying other alleles of Per2     (4 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Advanced Sleep Phase Syndrome, Familial, 1; FASPS1   (PER2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Per2tm1Drw/Per2tm1Drw

        involves: 129/Sv
  • behavior/neurological phenotype
  • abnormal circadian temperature homeostasis
    • the acrophase of core temperature is phase-advanced compared to in wild-type mice   (MGI Ref ID J:95746)
  • abnormal sleep pattern
    • at the midday 3-hour period, mice wake more than wild-type mice   (MGI Ref ID J:95746)
    • at midday, mice exhibit less REM sleep than wild-type mice   (MGI Ref ID J:95746)
    • during the 12-hour recovery after 6 hours of prolonged waking, mice exhibit less waking and more short wave sleep compared to similarly treated wild-type mice   (MGI Ref ID J:95746)
    • mice are awake more during the mid-third of the light period compared with wild-type mice   (MGI Ref ID J:95746)
  • advanced circadian phase
    • the acrophase of core temperature is phase-advanced compared to in wild-type mice   (MGI Ref ID J:95746)
  • arrhythmic circadian persistence
    • all mice become arrhythmic when transferred into dark-dark conditions although at different duration and extent of rhythm persistence   (MGI Ref ID J:69626)
  • homeostasis/metabolism phenotype
  • abnormal circadian temperature homeostasis
    • the acrophase of core temperature is phase-advanced compared to in wild-type mice   (MGI Ref ID J:95746)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Circadian Rhythms

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Per2tm1Drw
Allele Name targeted mutation 1, David R Weaver
Allele Type Targeted (knock-out)
Common Name(s) Per2-; Per2m; mPer2ldc;
Mutation Made By Shin Yamazaki,   Vanderbilt University
Strain of Origin129S4/SvJae
Gene Symbol and Name Per2, period circadian clock 2
Chromosome 1
Gene Common Name(s) FASPS; FASPS1; mKIAA0347; mPer2; rPER2;
Molecular Note Exon 5 and a portion of exon 6 were replaced with a neomycin selection cassette. Several transcript forms produced from the targeted allele were identified in homozygous mutant mice via RT-PCR and RACE analyses of brain RNA. The different transcripts involved aberrant splicing of exon 4 to a pseudoexon in intron 4, to a cryptic splice site within neo, and to exon 7. In spite of the transcripts having in-frame regions, normal protein was undetected in by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. [MGI Ref ID J:69626]

Genotyping

Genotyping Information

Genotyping Protocols

Per2tm1Drw, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bae K; Jin X; Maywood ES; Hastings MH; Reppert SM; Weaver DR. 2001. Differential Functions of mPer1, mPer2, and mPer3 in the SCN Circadian Clock. Neuron 30(2):525-36. [PubMed: 11395012]  [MGI Ref ID J:69626]

Additional References

Per2tm1Drw related

Anea CB; Ali MI; Osmond JM; Sullivan JC; Stepp DW; Merloiu AM; Rudic RD. 2010. Matrix metalloproteinase 2 and 9 dysfunction underlie vascular stiffness in circadian clock mutant mice. Arterioscler Thromb Vasc Biol 30(12):2535-43. [PubMed: 20829506]  [MGI Ref ID J:182097]

Bae K; Lee K; Seo Y; Lee H; Kim D; Choi I. 2006. Differential effects of two period genes on the physiology and proteomic profiles of mouse anterior tibialis muscles. Mol Cells 22(3):275-84. [PubMed: 17202855]  [MGI Ref ID J:135910]

Beaule C; Swanstrom A; Leone MJ; Herzog ED. 2009. Circadian modulation of gene expression, but not glutamate uptake, in mouse and rat cortical astrocytes. PLoS One 4(10):e7476. [PubMed: 19829696]  [MGI Ref ID J:154095]

Chen R; Schirmer A; Lee Y; Lee H; Kumar V; Yoo SH; Takahashi JS; Lee C. 2009. Rhythmic PER abundance defines a critical nodal point for negative feedback within the circadian clock mechanism. Mol Cell 36(3):417-30. [PubMed: 19917250]  [MGI Ref ID J:154857]

Cheng B; Anea CB; Yao L; Chen F; Patel V; Merloiu A; Pati P; Caldwell RW; Fulton DJ; Rudic RD. 2011. Tissue-intrinsic dysfunction of circadian clock confers transplant arteriosclerosis. Proc Natl Acad Sci U S A 108(41):17147-52. [PubMed: 21969583]  [MGI Ref ID J:177448]

Cho YH; Kim D; Choi I; Bae K. 2011. Identification of transcriptional regulatory elements required for the Mup2 expression in circadian clock mutant mice. Biochem Biophys Res Commun 410(4):834-40. [PubMed: 21703244]  [MGI Ref ID J:174778]

Duong HA; Robles MS; Knutti D; Weitz CJ. 2011. A molecular mechanism for circadian clock negative feedback. Science 332(6036):1436-9. [PubMed: 21680841]  [MGI Ref ID J:173532]

Grimaldi B; Bellet MM; Katada S; Astarita G; Hirayama J; Amin RH; Granneman JG; Piomelli D; Leff T; Sassone-Corsi P. 2010. PER2 controls lipid metabolism by direct regulation of PPARgamma. Cell Metab 12(5):509-20. [PubMed: 21035761]  [MGI Ref ID J:167907]

Gumz ML; Stow LR; Lynch IJ; Greenlee MM; Rudin A; Cain BD; Weaver DR; Wingo CS. 2009. The circadian clock protein Period 1 regulates expression of the renal epithelial sodium channel in mice. J Clin Invest 119(8):2423-34. [PubMed: 19587447]  [MGI Ref ID J:152557]

Hoogerwerf WA; Shahinian VB; Cornelissen G; Halberg F; Bostwick J; Timm J; Bartell PA; Cassone VM. 2010. Rhythmic changes in colonic motility are regulated by period genes. Am J Physiol Gastrointest Liver Physiol 298(2):G143-50. [PubMed: 19926812]  [MGI Ref ID J:157491]

Jang YS; Lee MH; Lee SH; Bae K. 2011. Cu/Zn superoxide dismutase is differentially regulated in period gene-mutant mice. Biochem Biophys Res Commun 409(1):22-7. [PubMed: 21549097]  [MGI Ref ID J:172369]

Lamia KA; Storch KF; Weitz CJ. 2008. Physiological significance of a peripheral tissue circadian clock. Proc Natl Acad Sci U S A 105(39):15172-7. [PubMed: 18779586]  [MGI Ref ID J:141274]

LeSauter J; Lambert CM; Robotham MR; Model Z; Silver R; Weaver DR. 2012. Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus. PLoS One 7(4):e35938. [PubMed: 22558277]  [MGI Ref ID J:187275]

Lee C; Weaver DR; Reppert SM. 2004. Direct association between mouse PERIOD and CKIepsilon is critical for a functioning circadian clock. Mol Cell Biol 24(2):584-94. [PubMed: 14701732]  [MGI Ref ID J:87576]

Lee H; Chen R; Lee Y; Yoo S; Lee C. 2009. Essential roles of CKIdelta and CKIepsilon in the mammalian circadian clock. Proc Natl Acad Sci U S A 106(50):21359-64. [PubMed: 19948962]  [MGI Ref ID J:155524]

Maywood ES; Drynan L; Chesham JE; Edwards MD; Dardente H; Fustin JM; Hazlerigg DG; O'Neill JS; Codner GF; Smyllie NJ; Brancaccio M; Hastings MH. 2013. Analysis of core circadian feedback loop in suprachiasmatic nucleus of mCry1-luc transgenic reporter mouse. Proc Natl Acad Sci U S A 110(23):9547-52. [PubMed: 23690615]  [MGI Ref ID J:197411]

Meng QJ; Maywood ES; Bechtold DA; Lu WQ; Li J; Gibbs JE; Dupre SM; Chesham JE; Rajamohan F; Knafels J; Sneed B; Zawadzke LE; Ohren JF; Walton KM; Wager TT; Hastings MH; Loudon AS. 2010. Entrainment of disrupted circadian behavior through inhibition of casein kinase 1 (CK1) enzymes. Proc Natl Acad Sci U S A 107(34):15240-5. [PubMed: 20696890]  [MGI Ref ID J:163807]

Park N; Cheon S; Son GH; Cho S; Kim K. 2012. Chronic circadian disturbance by a shortened light-dark cycle increases mortality. Neurobiol Aging 33(6):1122.e11-22. [PubMed: 22154820]  [MGI Ref ID J:188320]

Pendergast JS; Friday RC; Yamazaki S. 2010. Distinct functions of Period2 and Period3 in the mouse circadian system revealed by in vitro analysis. PLoS One 5(1):e8552. [PubMed: 20072700]  [MGI Ref ID J:157236]

Pendergast JS; Friday RC; Yamazaki S. 2010. Photic entrainment of period mutant mice is predicted from their phase response curves. J Neurosci 30(36):12179-84. [PubMed: 20826680]  [MGI Ref ID J:164289]

Pendergast JS; Oda GA; Niswender KD; Yamazaki S. 2012. Period determination in the food-entrainable and methamphetamine-sensitive circadian oscillator(s). Proc Natl Acad Sci U S A 109(35):14218-23. [PubMed: 22891330]  [MGI Ref ID J:188577]

Qu X; Metz RP; Porter WW; Cassone VM; Earnest DJ. 2007. Disruption of clock gene expression alters responses of the aryl hydrocarbon receptor signaling pathway in the mouse mammary gland. Mol Pharmacol 72(5):1349-58. [PubMed: 17715397]  [MGI Ref ID J:147684]

Shiromani PJ; Xu M; Winston EM; Shiromani SN; Gerashchenko D; Weaver DR. 2004. Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes. Am J Physiol Regul Integr Comp Physiol 287(1):R47-57. [PubMed: 15031135]  [MGI Ref ID J:95746]

Wang CY; Wen MS; Wang HW; Hsieh IC; Li Y; Liu PY; Lin FC; Liao JK. 2008. Increased vascular senescence and impaired endothelial progenitor cell function mediated by mutation of circadian gene Per2. Circulation 118(21):2166-73. [PubMed: 18981300]  [MGI Ref ID J:165618]

Xu Y; Toh KL; Jones CR; Shin JY; Fu YH; Ptacek LJ. 2007. Modeling of a human circadian mutation yields insights into clock regulation by PER2. Cell 128(1):59-70. [PubMed: 17218255]  [MGI Ref ID J:126404]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
   009104 129S4/SvJaeJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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