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| These Gfap-EGFP transgenic mice have EGFP expression directed primarily to Muller cells and may be useful in studies of retinal glial cells. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Vijay Sarthy, Northwestern University Description
Mice harboring the Gfap-EGFP transgene are viable and fertile. The mouse glial fibrillary acidic protein (Gfap) promoter region directs expression of enhanced green fluorescent protein (EGFP) primarily to adult mouse retinal Muller cells. Developmental expression of EGFP mostly parallels GFAP in retinal astrocytes. Expression of EGFP first appears in Muller cells around two weeks of age. However, not all Muller cells express EGFP.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
The Gfap-EGFP transgene was designed with a 2.5 kb 5' fragment of the mouse Gfap glial fibrillary acidic protein placed upstream of an enhanced green fluorescent protein (EGFP) coding sequence and SV40 polyA sequence. This transgene was microinjected into fertilized ICR(CD-1) mouse oocytes. Founder mice (line 3739) were mated with ICR(CD-1) mice for several generations and subsequently bred to C57BL/6 mice for 15 generations prior to arrival at The Jackson Laboratory. The Donating Investigator indicates that homozygotes are viable, but maintained the strain by mating hemizygotes to outbred/inbred mice. Upon arrival, transgenic mice were bred with C57BL/6J to establish the colony.SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (5 of 5 markers segregating) in the development of the strain.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Gfap-EGFP)3739Sart/0
involves: CD-1
- normal phenotype
- no abnormal phenotype detected
- mice are viable and fertile; no details of phenotypic analysis are provided (MGI Ref ID J:151687)
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Fluorescent Proteins
Sensorineural Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Gfap-EGFP)3739Sart | ||
|---|---|---|---|
| Allele Name | transgene insertion 3739, Vijay P Sarthy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GFAP-GFP; | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | Transgene expression is observed during retinal development, specifically in Muller cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gfap, glial fibrillary acidic protein, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the EGFP gene under the control of a 2.5 kb 5' fragment of the mouse Gfap, glial fibrillary acidic protein, promoter was injected into fertilized ICR(CD-1) mouse eggs. Founder line 3747 was established and characterized.Line 3739 was also established but not described in the original reference. Transgene expression is observed during retinal development, specifically in Muller cells. [MGI Ref ID J:151687] | ||
| Gene Symbol and Name | Tg(Gfap-EGFP)3739Sart, transgene insertion 3739, Vijay P Sarthy | ||
| Chromosome | UN | ||
| Gene Common Name(s) | GFAP-GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Gfap-EGFP)3739Sart, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kuzmanovic M; Dudley VJ; Sarthy VP. 2003. GFAP promoter drives Muller cell-specific expression in transgenic mice. Invest Ophthalmol Vis Sci 44(8):3606-13. [PubMed: 12882814] [MGI Ref ID J:151687]
Additional References
Tg(Gfap-EGFP)3739Sart relatedKaneko N; Marin O; Koike M; Hirota Y; Uchiyama Y; Wu JY; Lu Q; Tessier-Lavigne M; Alvarez-Buylla A; Okano H; Rubenstein JL; Sawamoto K. 2010. New neurons clear the path of astrocytic processes for their rapid migration in the adult brain. Neuron 67(2):213-23. [PubMed: 20670830] [MGI Ref ID J:167867]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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