Strain Name:

C;129-Crhr2tm1Jsp/J

Stock Number:

010902

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Availability:

Cryopreserved - Ready for recovery

Male mice lacking expression of corticotropin releasing hormone receptor 2 (Crhr2) exhibit enhanced anxious behavior in several tests of anxiety. Female targeted mutant mice do not show this increase of anxiety-like behavior.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Michael Rosenfeld,   University of California, San Diego

Description
Male mice lacking expression of corticotropin releasing hormone receptor 2 (Crhr2) exhibit enhanced anxious behavior in several tests of anxiety, including the elevated plus-maze test and the dark-light emergence task. Female targeted mutant mice do not show this increase of anxiety-like behavior. Mice show impaired responses in specific brain regions involved in emotional and autonomic function as monitored by a reduction of Creb phosphorylation in male, but not female, homozygous targeted mice. Male and female deficient animals display a larger edema response in response to thermal injury than do their wildtype littermates.

Development
Three exons encoding a cytoplasmic region (third intracellular loop) of the targeted gene were replaced with a neomycin resistance cassette. The (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cell line was used to create the mutation. Repeated backcrosses to C57BL/6 were found to contribute to a reduced recovery of homozygotes. The strain was backcrossed to BALB/c for an unspecified number of generations by the donating laboratory. Single nucleotide polymophism (SNP) analysis indicated a mixed genetic background upon importation.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Crhr2tm1Jsp allele
010842   B6;129-Crhr2tm1Jsp/J
View Strains carrying   Crhr2tm1Jsp     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Crhr2tm1Jsp/Crhr2+

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • male heterozygotes displayed normal locomotor activity in the elevated plus-maze test, and in a novel environment   (MGI Ref ID J:61485)
    • female heterozygotes showed normal baseline anxiety levels, stress-induced anxiety, and locomotor activity relative to wild-type   (MGI Ref ID J:61485)
    • baseline anxiety of female wild-type and heterozygous mice did not change with regard to estrous cycle phase   (MGI Ref ID J:61485)
    • female heterozygotes showed normal behavior in the dark-light emergence task, open-field and acute locomotor activity test   (MGI Ref ID J:61485)
    • increased anxiety-related response
      • in the elevated plus-maze, heterozygous males appeared to be more anxious than wild-type males   (MGI Ref ID J:61485)
      • in the dark-light emergence and open-field tests, the anxiety level of heterozygotes was between that of homozygous null and wild-type mice   (MGI Ref ID J:61485)
      • in the elevated plus-maze, the anxious behavior of heterozygotes was indistinguishable from that of homozygous null mice   (MGI Ref ID J:61485)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • heterozygotes displayed a normal activation of the HPA axis in response to 10 minutes of physical restraint stress   (MGI Ref ID J:61485)
    • the increased POMC1 (ACTH) and corticosterone levels produced by male or female heterozygotes did not differ from the values obtained for matched wild-type controls   (MGI Ref ID J:61485)

Crhr2tm1Jsp/Crhr2tm1Jsp

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • male and female homozygotes showed normal baseline feeding and body weight gain   (MGI Ref ID J:61485)
    • male homozygotes displayed normal locomotor activity in the elevated plus-maze test, and in a novel environment   (MGI Ref ID J:61485)
    • female homozygotes showed normal baseline anxiety levels, stress-induced anxiety, and locomotor activity relative to wild-type   (MGI Ref ID J:61485)
    • baseline anxiety of female wild-type and homozygous mice did not change with regard to estrous cycle phase   (MGI Ref ID J:61485)
    • female homozygotes showed normal behavior in the dark-light emergence task, open-field and acute locomotor activity test   (MGI Ref ID J:61485)
    • increased anxiety-related response
      • in the elevated plus-maze, homozygous males appeared to be more anxious than wild-type males   (MGI Ref ID J:61485)
      • in the open-field, homozygotes were explorative and did not differ from wild-type in traveled distance and time spent in the peripheral zones; however, homozygotes showed increased center region activity   (MGI Ref ID J:61485)
      • a single 5-min exposure to the elevated plus-maze resulted in a sustained elevation of phosphorylated Creb and widespread Fos production   (MGI Ref ID J:61485)
      • the levels of pCreb were lower in specific brain regions of male, but not female, homozygotes compared with wild-type controls: these regions included the cingulate cortex, the bed nucleus of the stria terminalis, the ventromedial hypothalamic nucleus and the basolateral amygdala   (MGI Ref ID J:61485)
      • all genotypes showed a similar level and distribution of Fos protein after exposure to the pus-maze regardless of gender   (MGI Ref ID J:61485)
  • cardiovascular system phenotype
  • abnormal vascular permeability
    • homozygotes of both genders exhibited increased edema formation in response to thermal injury relative to wild-type   (MGI Ref ID J:61485)
  • hearing/vestibular/ear phenotype
  • *normal* hearing/vestibular/ear phenotype
    • no auditory or orientational deficits   (MGI Ref ID J:61485)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • homozygotes displayed a normal activation of the HPA axis in response to 10 minutes of physical restraint stress   (MGI Ref ID J:61485)
    • the increased POMC1 (ACTH) and corticosterone levels produced by male or female homozygotes did not differ from the values obtained for matched wild-type controls   (MGI Ref ID J:61485)
  • vision/eye phenotype
  • *normal* vision/eye phenotype
    • no visual deficits   (MGI Ref ID J:61485)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Internal/Organ Research
Wound Healing

Neurobiology Research
Behavioral and Learning Defects
      high anxiety

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Crhr2tm1Jsp
Allele Name targeted mutation 1, Joachim Speiss
Allele Type Targeted (Null/Knockout)
Common Name(s) Crhr2-;
Mutation Made By Joachim Spiess,   Salk Institute
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Crhr2, corticotropin releasing hormone receptor 2
Chromosome 6
Gene Common Name(s) CRF 2 receptor; CRF-R2; CRF-RB; CRF2; CRFR2; CRFR2alpha; CRFR2beta; CRH-R2; Crf2r; HM-CRF;
Molecular Note A neomycin selection cassette replaced a genomic fragment containing exons which encode the third intracelllular loop. Binding assays on sections of adult homozygous brain confirmed that no functional protein was expressed from this allele. No detectable transcript was produced from this allele (data not shown). [MGI Ref ID J:61485]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kishimoto T; Radulovic J; Radulovic M; Lin CR; Schrick C; Hooshmand F; Hermanson O; Rosenfeld MG; Spiess J. 2000. Deletion of crhr2 reveals an anxiolytic role for corticotropin-releasing hormone receptor-2. Nat Genet 24(4):415-9. [PubMed: 10742109]  [MGI Ref ID J:61485]

Additional References

Crhr2tm1Jsp related

Stiedl O; Meyer M. 2003. Cardiac dynamics in corticotropin-releasing factor receptor subtype-2 deficient mice. Neuropeptides 37(1):3-16. [PubMed: 12637030]  [MGI Ref ID J:119312]

Stiedl O; Meyer M; Kishimoto T; Rosenfeld MG; Spiess J. 2003. Stress-mediated heart rate dynamics after deletion of the gene encoding corticotropin-releasing factor receptor 2. Eur J Neurosci 17(10):2231-5. [PubMed: 12786991]  [MGI Ref ID J:89671]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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