Description

Strain Information

Former Names B6.Cg-Tg(Sry)2Ei Srydl1Rob/ArnoJ    (Changed: 29-SEP-09 ) Type Congenic; Mutant Strain; Spontaneous Mutation; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N18+N4 (27-JUN-11) Generation Definitions   Donating Investigator Arthur P Arnold,   UCLA

Description
The dl1Rlb allele (Y^-) is an 11 kb deletion in the sex determining region of the Y chromosome, Sry , XY^- mice (with ovaries) with this mutation are phenotypic gonadal females, although they lose germ cells and cease estrous cycling earlier in life. The donating investigator indicates that XY^- mice generally infertile on the C57BL/6 background . XX mice carrying the Tg(Sry)2Ei transgene are phenotypic gonadal males (with testes), although they lack sperm and have smaller testes than normal males. When the two mutations are combined, testis determination is transferred from the Y chromosome to an autosome. Mating the carrier male to a C57BL/6J female produces four "core" genotypes that can be used as a model to investigate relationships between sex chromosome complement (XX or XY) and gonadal type that influences phenotypic characteristics. The four genotypes produced are two types of gonadal females (XX, XY^-), and two types of gonadal males (XXTg(Sry), XY^- Tg(Sry)).

Development
The Tg(Sry)2Ei transgenic construct was generated by Dr. Robin Lovell-Badge's laboratory from 129-derived 14.6-kb of genomic sequence encoding Sry. In the laboratory of Dr. Eva Eicher at the Jackson Laboratory, the construct was injected into the male pronuclei of fertilized eggs obtained from mating C57BL/6JEi females with C57BL/6JEi-Chr Y^AKR/J males. The XY transgenic male founder 2 was obtained and maintained by backcrossing transgenic males, obligatorily carrying the consomic Chr Y^AKR/J, to C57BL/6JEi females.

The dl1Rlb mutation arose spontaneously in a 129S6/SvEvTac derived-CCE embryonic stem cell line treated for retroviral integration. Chimeric male AL430 was mated to females from a C3H based random bred colony carrying the blotchy mutation. XY females produced by these matings were determined to be the result of a spontaneous 11 kb deletion in the Y chromosome and not a proviral insertion. These XY females were either sterile or extremely subfertile, and the continued maintenance of the stock was dependent on fertile XXY females generated by Dr. Paul Burgoyne. This mutation, originally termed Tdy^m1,, was first characterized in the laboratory of Dr. Robin Lovell-Badge, National Institute of Medical Research, London. The dl1Rlb mutation was transferred to an outbred MF1 background and then eventually combined with the Tg(Sry)2Ei transgene by Paul Burgoyne at NIMR, who mated XXY females (that generate X and XY eggs) to X (Paf labeled) Y transgenic males (that generate a low frequency of 'O' gametes with the Sry transgene) to produce the synthetically fertile XY dl1Rlb:Tg(Sry)2Ei males. When mated to normal XX females, the only fertile male offspring are those with the same genotype as the father. The mutation and transgene were backcrossed together into the C57BL/6J background for a minimum of eighteen generations. Dr. Arthur Arnold, UCLA, donated the strain to the Repository in 2009.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Sry)2Ei allele

006305	B6Ei.Cg-Nr0b1tm1.1Lja Tg(Sry)2Ei Chr YAKR/EiJ
002708	C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/J/EiJ

View Strains carrying   Tg(Sry)2Ei     (2 strains)

Strains carrying other alleles of Sry

007484	B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007265	C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ
007264	C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
003927	C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007250	C57BL/6JEi-Chr YAKR/J/Ei
001543	C57BL/6JEi-Chr YCB/EiJ
002063	C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ
002837	C57BL/6JEi-Tg(Sry)3Ei Chr YAKR/J/EiJ
002183	C57BL/6JEi-Tg(Sry)4Ei Chr YPOS/EiJ
007483	FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ

View Strains carrying other alleles of Sry     (10 strains)

Additional Web Information

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. 46,xx Sex Reversal 1; SRXX1   (SRY) 46,xx Sex Reversal 2; SRXX2   (SRY) 46,xy Sex Reversal 1; SRXY1   (SRY)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

X/Sry^dl1Rlb Tg(Sry)2Ei/0

        involves: 129S/SvEv * C3H * C57BL/6JEi * MF1

• reproductive system phenotype
• primary sex reversal
  • chromosomal males that would have developed as fertile females develop as males   (MGI Ref ID J:89312)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      gonads

Reproductive Biology Research
Developmental Defects Affecting Gonads

Tg(Sry)2Ei related

Developmental Biology Research
Internal/Organ Defects
      gonads

Reproductive Biology Research
Developmental Defects Affecting Gonads

Research Tools
Developmental Biology Research
Reproductive Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Srydl1Rlb
Allele Name		sex determining region of Chr Y-deletion 1, Robin Lovell-Badge
Allele Type		Spontaneous
Common Name(s)		Srydl1Rob; Srym1; Tdym1; Y-; YTdym1; Yq-del;
Strain of Origin		129S6/SvEvTac
Gene Symbol and Name		Sry, sex determining region of Chr Y
Chromosome		Y
Gene Common Name(s)		Tdf; Tdy; testis determining factor; testis determining-Y;
Molecular Note		This allele contains an 11 kb deletion in the testis-determining region of the gene, including a highly conserved exon encoding the HMG box domain. [MGI Ref ID J:2258]
Allele Symbol		Tg(Sry)2Ei
Allele Name		transgene insertion 2, Eva M Eicher
Allele Type		Transgenic (random, expressed)
Common Name(s)		Tg(Sry-129)2Ei;
Strain of Origin		C57BL/6JEi-Chr Y
Expressed Gene		Sry, sex determining region of Chr Y, mouse, laboratory
Promoter		Sry, sex determining region of Chr Y, mouse, laboratory
Molecular Note		This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. [MGI Ref ID J:71077]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Sry)Eicher, Standard PCR
Sry, QPCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Arnold AP. 2009. Mouse models for evaluating sex chromosome effects that cause sex differences in non-gonadal tissues. J Neuroendocrinol 21(4):377-86. [PubMed: 19207816]  [MGI Ref ID J:152290]

Arnold AP; Chen X. 2009. What does the 'four core genotypes' mouse model tell us about sex differences in the brain and other tissues? Front Neuroendocrinol 30(1):1-9. [PubMed: 19028515]  [MGI Ref ID J:152291]

Additional References

Sry^dl1Rlb related

Arnold AP; Xu J; Grisham W; Chen X; Kim YH; Itoh Y. 2004. Minireview: Sex chromosomes and brain sexual differentiation. Endocrinology 145(3):1057-62. [PubMed: 14670983]  [MGI Ref ID J:88705]

Carruth LL; Reisert I; Arnold AP. 2002. Sex chromosome genes directly affect brain sexual differentiation. Nat Neurosci 5(10):933-4. [PubMed: 12244322]  [MGI Ref ID J:130009]

Chen X; McClusky R; Chen J; Beaven SW; Tontonoz P; Arnold AP; Reue K. 2012. The number of x chromosomes causes sex differences in adiposity in mice. PLoS Genet 8(5):e1002709. [PubMed: 22589744]  [MGI Ref ID J:185119]

Cocquet J; Ellis PJ; Yamauchi Y; Mahadevaiah SK; Affara NA; Ward MA; Burgoyne PS. 2009. The multicopy gene Sly represses the sex chromosomes in the male mouse germline after meiosis. PLoS Biol 7(11):e1000244. [PubMed: 19918361]  [MGI Ref ID J:155571]

Cox KH; Rissman EF. 2011. Sex differences in juvenile mouse social behavior are influenced by sex chromosomes and social context. Genes Brain Behav 10(4):465-72. [PubMed: 21414140]  [MGI Ref ID J:185705]

De Vries GJ; Rissman EF; Simerly RB; Yang LY; Scordalakes EM; Auger CJ; Swain A; Lovell-Badge R; Burgoyne PS; Arnold AP. 2002. A model system for study of sex chromosome effects on sexually dimorphic neural and behavioral traits. J Neurosci 22(20):9005-14. [PubMed: 12388607]  [MGI Ref ID J:79778]

Durcova-Hills G; Hajkova P; Sullivan S; Barton S; Surani MA; McLaren A. 2006. Influence of sex chromosome constitution on the genomic imprinting of germ cells. Proc Natl Acad Sci U S A 103(30):11184-8. [PubMed: 16847261]  [MGI Ref ID J:111825]

Gatewood JD; Wills A; Shetty S; Xu J; Arnold AP; Burgoyne PS; Rissman EF. 2006. Sex chromosome complement and gonadal sex influence aggressive and parental behaviors in mice. J Neurosci 26(8):2335-42. [PubMed: 16495461]  [MGI Ref ID J:105767]

Gubbay J; Vivian N; Economou A; Jackson D; Goodfellow P; Lovell-Badge R. 1992. Inverted repeat structure of the Sry locus in mice. Proc Natl Acad Sci U S A 89(17):7953-7. [PubMed: 1518820]  [MGI Ref ID J:2258]

Kim Y; Kobayashi A; Sekido R; DiNapoli L; Brennan J; Chaboissier MC; Poulat F; Behringer RR; Lovell-Badge R; Capel B. 2006. Fgf9 and Wnt4 act as antagonistic signals to regulate mammalian sex determination. PLoS Biol 4(6):e187. [PubMed: 16700629]  [MGI Ref ID J:110252]

Lovell-Badge R; Robertson E. 1990. XY female mice resulting from a heritable mutation in the primary testis-determining gene, Tdy. Development 109(3):635-46. [PubMed: 2401216]  [MGI Ref ID J:10734]

Mahadevaiah SK; Odorisio T; Elliott DJ; Rattigan A; Szot M; Laval SH ; Washburn LL ; McCarrey JR ; Cattanach BM ; Lovell-Badge R ; Burgoyne PS. 1998. Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 7(4):715-27. [PubMed: 9499427]  [MGI Ref ID J:46791]

Park JH; Burns-Cusato M; Dominguez-Salazar E; Riggan A; Shetty S; Arnold AP; Rissman EF. 2008. Effects of sex chromosome aneuploidy on male sexual behavior. Genes Brain Behav 7(6):609-17. [PubMed: 18363850]  [MGI Ref ID J:151160]

Sekido R; Bar I; Narvaez V; Penny G; Lovell-Badge R. 2004. SOX9 is up-regulated by the transient expression of SRY specifically in Sertoli cell precursors. Dev Biol 274(2):271-9. [PubMed: 15385158]  [MGI Ref ID J:93564]

Szot M; Grigoriev V; Mahadevaiah SK; Ojarikre OA; Toure A; von Glasenapp E; Rattigan A; Turner JM; Elliott DJ; Burgoyne PS. 2003. Does Rbmy have a role in sperm development in mice? Cytogenet Genome Res 103(3-4):330-6. [PubMed: 15051956]  [MGI Ref ID J:89312]

Toure A; Clemente EJ; Ellis P; Mahadevaiah SK; Ojarikre OA; Ball PA; Reynard L; Loveland KL; Burgoyne PS; Affara NA. 2005. Identification of novel Y chromosome encoded transcripts by testis transcriptome analysis of mice with deletions of the Y chromosome long arm. Genome Biol 6(12):R102. [PubMed: 16356265]  [MGI Ref ID J:104746]

Toure A; Szot M; Mahadevaiah SK; Rattigan A; Ojarikre OA; Burgoyne PS. 2004. A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 166(2):901-12. [PubMed: 15020475]  [MGI Ref ID J:89616]

Wagner CK; Xu J; Pfau JL; Quadros PS; De Vries GJ; Arnold AP. 2004. Neonatal mice possessing an Sry transgene show a masculinized pattern of progesterone receptor expression in the brain independent of sex chromosome status. Endocrinology 145(3):1046-9. [PubMed: 14645115]  [MGI Ref ID J:88702]

Ward MA; Burgoyne PS. 2006. The Effects of Deletions of the Mouse Y Chromosome Long Arm on Sperm Function--Intracytoplasmic Sperm Injection (ICSI)-Based Analysis. Biol Reprod 74(4):652-8. [PubMed: 16354792]  [MGI Ref ID J:107012]

Yamauchi Y; Riel JM; Stoytcheva Z; Burgoyne PS; Ward MA. 2010. Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage. Genome Biol 11(6):R66. [PubMed: 20573212]  [MGI Ref ID J:179194]

Tg(Sry)2Ei related

Albrecht KH; Young M; Washburn LL; Eicher EM. 2003. Sry expression level and protein isoform differences play a role in abnormal testis development in C57BL/6J mice carrying certain Sry alleles. Genetics 164(1):277-88. [PubMed: 12750339]  [MGI Ref ID J:127090]

Carruth LL; Reisert I; Arnold AP. 2002. Sex chromosome genes directly affect brain sexual differentiation. Nat Neurosci 5(10):933-4. [PubMed: 12244322]  [MGI Ref ID J:130009]

Gatewood JD; Wills A; Shetty S; Xu J; Arnold AP; Burgoyne PS; Rissman EF. 2006. Sex chromosome complement and gonadal sex influence aggressive and parental behaviors in mice. J Neurosci 26(8):2335-42. [PubMed: 16495461]  [MGI Ref ID J:105767]

Mahadevaiah SK; Odorisio T; Elliott DJ; Rattigan A; Szot M; Laval SH ; Washburn LL ; McCarrey JR ; Cattanach BM ; Lovell-Badge R ; Burgoyne PS. 1998. Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 7(4):715-27. [PubMed: 9499427]  [MGI Ref ID J:46791]

Quinn JJ; Hitchcott PK; Umeda EA; Arnold AP; Taylor JR. 2007. Sex chromosome complement regulates habit formation. Nat Neurosci 10(11):1398-400. [PubMed: 17952068]  [MGI Ref ID J:128462]

Smith-Bouvier DL; Divekar AA; Sasidhar M; Du S; Tiwari-Woodruff SK; King JK; Arnold AP; Singh RR; Voskuhl RR. 2008. A role for sex chromosome complement in the female bias in autoimmune disease. J Exp Med 205(5):1099-108. [PubMed: 18443225]  [MGI Ref ID J:136240]

Szot M; Grigoriev V; Mahadevaiah SK; Ojarikre OA; Toure A; von Glasenapp E; Rattigan A; Turner JM; Elliott DJ; Burgoyne PS. 2003. Does Rbmy have a role in sperm development in mice? Cytogenet Genome Res 103(3-4):330-6. [PubMed: 15051956]  [MGI Ref ID J:89312]

Toure A; Szot M; Mahadevaiah SK; Rattigan A; Ojarikre OA; Burgoyne PS. 2004. A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 166(2):901-12. [PubMed: 15020475]  [MGI Ref ID J:89616]

Wagner CK; Xu J; Pfau JL; Quadros PS; De Vries GJ; Arnold AP. 2004. Neonatal mice possessing an Sry transgene show a masculinized pattern of progesterone receptor expression in the brain independent of sex chromosome status. Endocrinology 145(3):1046-9. [PubMed: 14645115]  [MGI Ref ID J:88702]

Ward MA; Burgoyne PS. 2006. The Effects of Deletions of the Mouse Y Chromosome Long Arm on Sperm Function--Intracytoplasmic Sperm Injection (ICSI)-Based Analysis. Biol Reprod 74(4):652-8. [PubMed: 16354792]  [MGI Ref ID J:107012]

Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455]  [MGI Ref ID J:71077]

Yamauchi Y; Riel JM; Stoytcheva Z; Burgoyne PS; Ward MA. 2010. Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage. Genome Biol 11(6):R66. [PubMed: 20573212]  [MGI Ref ID J:179194]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Breeding & Husbandry	While maintaining a live colony, these mice are bred as carrier male crossed to C57BL/6 female.
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Use restrictions apply, see Policy on Licensing and Use Restrictions.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.