Strain Name:

STOCK Wt1tm2(cre/ERT2)Wtp/J

Stock Number:

010912

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Common Names: Wt1CreERT2;    
The Wt1CreERT2 knock-in allele in these mice both abolishes Wilms tumor 1 homolog gene function and directs expression of the CreERT2 fusion protein to proepicardium and epicardium by the Wt1 promoter/enhancer elements. As Wt1 is expressed in the developing genitourinary system and in the mesothelia overlying most visceral organs, these mutant mice are useful as a tamoxifen-inducible Cre-lox tool for lineage-tracing/marking Wt1-expressing cells for studying cardiomyocytes in the developing heart.

Description

Strain Information

Type Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHeterozygote x +/+ sibling         (Female x Male)   23-MAR-10
Specieslaboratory mouse
GenerationF?+F12 (15-MAY-15)
Generation Definitions
 
Donating Investigator William T Pu,   Children's Hospital Boston, Harvard MS

Description
Homozygous mice die between embryonic day (E)13.5 and birth with defects of heart, kidney, gonads, and multiple other organs. Heterozygous (Wt1CreERT2/+) mice are viable and fertile. The Wt1CreERT2 "knock-in" allele both abolishes Wt1 gene function and has expression of the CreERT2 fusion protein (CreERT2) under control of the Wt1 promoter/enhancer elements. In heart from heterozygous mice, CreERT2 expression is directed to proepicardium and epicardium from E9.5 to E15.5, and is not found in the myocardium. CreERT2 fusion gene activity is inducible; observed following tamoxifen administration. As such, when Wt1CreERT2 mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Wt1-expressing cells of the offspring. The donating investigator reports that Cre activity may be observed prior to tamoxifen exposure only in epicardium of adult mice following myocardial infarction. As Wt1 is expressed in the developing genitourinary system and in the mesothelia overlying most visceral organs, these mutant mice are useful as a tamoxifen-inducible Cre-lox tool for lineage-tracing/marking Wt1-expressing cells for studying cardiomyocytes in the developing heart.

The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development
A targeting vector was designed to replace exon 1 of the Wt1 (Wilms tumor 1 homolog) locus with a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked pgk-Neo cassette. The donating investigator reports that this construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with outbred Swiss Webster (CFW) mice to originate the colony. To remove the pgk-Neo cassette, mutant mice were bred with Actin-FLPe mice (mostly C57BL/6 genetic background; see Stock No. 005703). The resulting Wt1CreERT2 mice were subsequently mated with outbred Swiss Webster (CFW) mice for several generations (and the FLPe transgene was removed) prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred with B6129SF1/J hybrid mice (Stock No. 101043) for at least one generation to establish the Wt1CreERT2 colony.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Wt1
002719   B6.129S4-Wt1tm1Jae/J
002332   B6;129S4-Wt1tm1Jae/J
006817   D2.129S4(Cg)-Wt1tm1Jae/EiJ
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
View Strains carrying other alleles of Wt1     (4 strains)

Strains carrying other alleles of cre/ERT2
017611   129-Mcm2tm1(cre/ERT2)Scpr/J
009575   B6(129S4)-Et(cre/ERT2)119Rdav/J
009580   B6(129S4)-Et(cre/ERT2)1382Rdav/J
012688   B6(129S4)-Et(cre/ERT2)13866Rdav/J
009581   B6(129S4)-Et(cre/ERT2)1642Rdav/J
009582   B6(129S4)-Et(cre/ERT2)1645Rdav/J
009583   B6(129S4)-Et(cre/ERT2)1957Rdav/J
009584   B6(129S4)-Et(cre/ERT2)2007Rdav/J
009585   B6(129S4)-Et(cre/ERT2)2047Rdav/J
009574   B6(129S4)-Et(cre/ERT2)21Rdav/J
009577   B6(129S4)-Et(cre/ERT2)296Rdav/J
009578   B6(129S4)-Et(cre/ERT2)398Rdav/J
009573   B6(129S4)-Et(cre/ERT2)4Rdav/J
010688   B6(129S4)-Et(cre/ERT2)6691Rdav/J
010689   B6(129S4)-Et(cre/ERT2)6959Rdav/J
010690   B6(129S4)-Et(cre/ERT2)7089Rdav/J
010691   B6(129S4)-Et(cre/ERT2)7149Rdav/J
010692   B6(129S4)-Et(cre/ERT2)7381Rdav/J
010693   B6(129S4)-Et(cre/ERT2)8120Rdav/J
010694   B6(129S4)-Et(cre/ERT2)8131Rdav/J
009579   B6(129S4)-Et(cre/ERT2)837Rdav/J
010695   B6(129S4)-Et(cre/ERT2)9699Rdav/J
010696   B6(129S4)-Et(icre/ERT2)10596Rdav/J
010697   B6(129S4)-Et(icre/ERT2)10727Rdav/J
012689   B6(129S4)-Et(icre/ERT2)14163Rdav/J
012690   B6(129S4)-Et(icre/ERT2)14208Rdav/J
012694   B6(129S4)-Et(icre/ERT2)14915Rdav/J
022356   B6(129X1)-Tg(Cd4-cre/ERT2)11Gnri/J
013730   B6(Cg)-Calb2tm2.1(cre/ERT2)Zjh/J
010705   B6(Cg)-Dlx5tm1(cre/ERT2)Zjh/J
013048   B6(Cg)-Etv1tm1.1(cre/ERT2)Zjh/J
010776   B6(Cg)-Lhx6tm1(cre/ERT2)Zjh/J
010777   B6(Cg)-Pvalbtm1(cre/ERT2)Zjh/J
010708   B6(Cg)-Ssttm1(cre/ERT2)Zjh/J
026512   B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J
021881   B6.129(Cg)-Arctm1.1(cre/ERT2)Luo/J
018867   B6.129(Cg)-Axin2tm1(cre/ERT2)Rnu/J
021882   B6.129(Cg)-Fostm1.1(cre/ERT2)Luo/J
008463   B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J
020940   B6.129P2(C)-Cx3cr1tm2.1(cre/ERT2)Jung/J
021160   B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT)Litt/WganJ
027116   B6.129P2-Cd19tm1(cre/ERT2)Rsky/J
008875   B6.129P2-Lgr5tm1(cre/ERT2)Cle/J
016934   B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
016222   B6.129S(Cg)-Id2tm1.1(cre/ERT2)Blh/ZhuJ
005623   B6.129S6-Shhtm2(cre/ERT2)Cjt/J
019148   B6.Cg-Acantm1(cre/ERT2)Crm/J
012360   B6.Cg-Erbb4tm1.1(cre/ERT2)Aibs/J
023676   B6.Cg-Hprttm331(Ple275-icre/ERT2)Ems/Mmjax
023677   B6.Cg-Hprttm332(Ple272-icre/ERT2)Ems/Mmjax
023678   B6.Cg-Hprttm333(Ple281-icre/ERT2)Ems/Mmjax
023679   B6.Cg-Hprttm334(Ple279-icre/ERT2)Ems/Mmjax
023680   B6.Cg-Hprttm335(Ple277-icre/ERT2)Ems/Mmjax
023681   B6.Cg-Hprttm336(Ple134-icre/ERT2)Ems/Mmjax
023682   B6.Cg-Hprttm337(Ple285-icre/ERT2)Ems/Mmjax
023683   B6.Cg-Hprttm338(Ple284-icre/ERT2)Ems/Mmjax
023684   B6.Cg-Hprttm339(Ple280-icre/ERT2)Ems/Mmjax
023685   B6.Cg-Hprttm340(Ple252-icre/ERT2)Ems/Mmjax
023686   B6.Cg-Hprttm341(Ple273-icre/ERT2)Ems/Mmjax
023687   B6.Cg-Hprttm342(Ple282-icre/ERT2)Ems/Mmjax
023688   B6.Cg-Hprttm343(Ple270-icre/ERT2)Ems/Mmjax
023689   B6.Cg-Hprttm344(Ple5-icre/ERT2)Ems/Mmjax
023690   B6.Cg-Hprttm345(icre/ERT2)Ems/Mmjax
023691   B6.Cg-Hprttm346(Ple264-icre/ERT2)Ems/Mmjax
023692   B6.Cg-Hprttm347(Ple155-icre/ERT2)Ems/Mmjax
023693   B6.Cg-Hprttm348(Ple265-icre/ERT2)Ems/Mmjax
023694   B6.Cg-Hprttm349(Ple293-icre/ERT2)Ems/Mmjax
023695   B6.Cg-Hprttm350(Ple232-icre/ERT2)Ems/Mmjax
023696   B6.Cg-Hprttm351(Ple198-icre/ERT2)Ems/Mmjax
023697   B6.Cg-Hprttm352(Ple291-icre/ERT2)Ems/Mmjax
022861   B6.Cg-Nxph4tm1.1(cre/ERT2)Hze/J
017763   B6.Cg-Pax7tm1(cre/ERT2)Gaka/J
022862   B6.Cg-Penktm1.1(cre/ERT2)Hze/J
021189   B6.Cg-Pvalbtm1.1(cre/ERT2)Hze/J
022865   B6.Cg-Trib2tm1.1(cre/ERT2)Hze/J
022357   B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ
026516   B6.Cg-Tg(Avil-cre/ERT2)AJwo/J
022390   B6.Cg-Tg(CDX2-Cre/ERT2)752Erf/J
016241   B6.Cg-Tg(Col1a1-cre/ERT2)1Crm/J
024709   B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J
007606   B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J
019079   B6.FVB-Tg(Myh11-cre/ERT2)1Soff/J
021961   B6;129-Abcg2tm3.1(cre/ERT2)Bsor/J
010531   B6;129-Bmi1tm1(cre/ERT)Mrc/J
012476   B6;129-Pax7tm2.1(cre/ERT2)Fan/J
009600   B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J
010985   B6;129P-Klf3tm1(cre/ERT2)Pzg/J
015854   B6;129P2-Foxl2tm1(GFP/cre/ERT2)Pzg/J
014541   B6;129S-Nos1tm1.1(cre/ERT2)Zjh/J
010987   B6;129S-Sox18tm1(GFP/cre/ERT2)Pzg/J
017593   B6;129S-Sox2tm1(cre/ERT2)Hoch/J
014551   B6;129S4-Dlx1tm1(cre/ERT2)Zjh/J
012464   B6;129S4-Foxd1tm2(GFP/cre/ERT2)Amc/J
009576   B6;129S4-Et(cre/ERT2)278Rdav/J
024948   B6;129S6-Gdnftm1(cre/ERT2)Cos/J
012362   B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J
017495   B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J
014638   B6;129X1-Cldn6tm1(cre/ERT2)Dam/J
018937   B6;C-Tg(Erv4-cre/ERT2*)657Slp/J
025809   B6;CBA-Tg(Fgfr3-icre/ERT2)4-2Wdr/J
025808   B6;CBA-Tg(Nkx2-1-cre/ERT2)1Kess/J
024768   B6;CBA-Tg(Upk2-icre/ERT2)1Ccc/J
017496   B6;D-Tg(Lfng-TagRFP/cre/ERT2)8Amc/J
018682   B6;D-Tg(Meox1-TagRFP/cre/ERT2)30Amc/J
018683   B6;D-Tg(Wnt11-TagRFP/cre/ERT2)28Amc/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
010550   B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J
017743   B6N;129S-Prom1tm1(cre/ERT2)Gilb/J
025124   C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J
025867   C57BL/6J-Tg(Neurod1-cre/ERT2)M1Fcal/J
024760   FVB/N-Tg(Ddx4-cre/ERT2)1Dcas/J
012882   STOCK Ascl1tm1.1(Cre/ERT2)Jejo/J
012710   STOCK Ccktm2.1(cre/ERT2)Zjh/J
007924   STOCK En2tm4(cre/ERT2)Alj/J
016961   STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J
010702   STOCK Gad2tm1(cre/ERT2)Zjh/J
022135   STOCK Gbx2tm1.1(cre/ERT2)Jyhl/J
007913   STOCK Gli1tm3(cre/ERT2)Alj/J
024283   STOCK Hcn4tm2.1(cre/ERT2)Sev/J
017606   STOCK Hopxtm2.1(cre/ERT2)Joe/J
018418   STOCK Lrig1tm1.1(cre/ERT2)Rjc/J
014552   STOCK Nkx2-1tm1.1(cre/ERT2)Zjh/J
017536   STOCK Nkx6-2tm1(cre/ERT2)Fsh/J
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
017569   STOCK Polr2atm1(cre/ERT2)Bbd E4f1tm1.1Llca/J
017585   STOCK Polr2atm1(cre/ERT2)Bbd/J
022757   STOCK Prg4tm1(GFP/cre/ERT2)Abl/J
012691   STOCK Et(icre/ERT2)14374Rdav/J
012692   STOCK Et(icre/ERT2)14602Rdav/J
012693   STOCK Et(icre/ERT2)14624Rdav/J
008861   STOCK Tg(Ela1-Cre/ERT2)1Stof/J
022763   STOCK Tg(Eno2-cre/ERT2)1Pohlk/J
027681   STOCK Tg(Hnf1b-cre/ERT2)1Jfer/J
019380   STOCK Tg(IVL-cre/ERT2)1Blpn/J
017948   STOCK Tg(KRT18-cre/ERT2)23Blpn/J
017947   STOCK Tg(Krt8-cre/ERT2)17Blpn/J
018829   STOCK Tg(Sox9-cre/ERT2)1Msan/J
025520   STOCK Tg(T-cre/ERT2)1Lwd/J
View Strains carrying other alleles of cre/ERT2     (138 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Aniridia; AN   (WT1)
Denys-Drash Syndrome; DDS   (WT1)
Frasier Syndrome   (WT1)
Meacham Syndrome   (WT1)
Mesothelioma, Malignant; MESOM   (WT1)
Nephrotic Syndrome, Type 4; NPHS4   (WT1)
Wilms Tumor 1; WT1   (WT1)
Wilms Tumor, Aniridia, Genitourinary Anomalies, and Mental Retardation Syndrome; WAGR   (WT1)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities

Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects
      gonads
      heart
      kidney
      kidneys, gonads, heart

Internal/Organ Research
Heart Abnormalities
Kidney Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      Cre Recombinase Expression
      Cre Recombinase Expression: Inducible
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System
      Tissue/Cell Markers
      Tissue/Cell Markers: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Wt1tm2(cre/ERT2)Wtp
Allele Name targeted mutation 2, William T Pu
Allele Type Targeted (Inducible, cre- or Flp-expressing)
Common Name(s) Wt1CreERT2; Wt1iCre (i represents inducible); Wt1tm2(cre/ESR1)Wtp;
Mutation Made By William Pu,   Children's Hospital Boston, Harvard MS
Strain of Origin129S4/SvJae
Site of ExpressionCre activity is observed prior to tamoxifen exposure only in epicardium of adult mice following myocardial infarction.
Expressed Gene cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Gene Symbol and Name Wt1, Wilms tumor 1 homolog
Chromosome 2
Gene Common Name(s) AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; RIKEN cDNA D630046I19 gene; WAGR; WIT-2; WT33; Wt-1;
Driver Note Wt1
Inducible Note induced by tamoxifen
Molecular Note Exon 1 was replaced with an inducible cre ESR1 (ERT2) fusion followed by an frt-flanked neo cassette. Germ line, flp-mediated recombination was used to remove the neo cassette. [MGI Ref ID J:137426]
 

Genotyping

Genotyping Information

Genotyping Protocols

Wt1tm2(cre/ERT2)WtpqPCR, QPCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026]  [MGI Ref ID J:137426]

Additional References

Wt1tm2(cre/ERT2)Wtp related

Asahina K; Zhou B; Pu WT; Tsukamoto H. 2011. Septum transversum-derived mesothelium gives rise to hepatic stellate cells and perivascular mesenchymal cells in developing mouse liver. Hepatology 53(3):983-95. [PubMed: 21294146]  [MGI Ref ID J:170930]

Baek ST; Tallquist MD. 2012. Nf1 limits epicardial derivative expansion by regulating epithelial to mesenchymal transition and proliferation. Development 139(11):2040-9. [PubMed: 22535408]  [MGI Ref ID J:183991]

Barnes RM; Firulli BA; Vandusen NJ; Morikawa Y; Conway SJ; Cserjesi P; Vincentz JW; Firulli AB. 2011. Hand2 Loss-of-Function in Hand1-Expressing Cells Reveals Distinct Roles in Epicardial and Coronary Vessel Development. Circ Res 108(8):940-9. [PubMed: 21350214]  [MGI Ref ID J:171088]

Dixit R; Ai X; Fine A. 2013. Derivation of lung mesenchymal lineages from the fetal mesothelium requires hedgehog signaling for mesothelial cell entry. Development 140(21):4398-406. [PubMed: 24130328]  [MGI Ref ID J:204973]

Greif DM; Kumar M; Lighthouse JK; Hum J; An A; Ding L; Red-Horse K; Espinoza FH; Olson L; Offermanns S; Krasnow MA. 2012. Radial construction of an arterial wall. Dev Cell 23(3):482-93. [PubMed: 22975322]  [MGI Ref ID J:189012]

Hu YC; Okumura LM; Page DC. 2013. Gata4 is required for formation of the genital ridge in mice. PLoS Genet 9(7):e1003629. [PubMed: 23874227]  [MGI Ref ID J:199295]

Li Y; Wang J; Asahina K. 2013. Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury. Proc Natl Acad Sci U S A 110(6):2324-9. [PubMed: 23345421]  [MGI Ref ID J:194339]

Manuylov NL; Zhou B; Ma Q; Fox SC; Pu WT; Tevosian SG. 2011. Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol 353(2):229-41. [PubMed: 21385577]  [MGI Ref ID J:173838]

Miyoshi H; Ajima R; Luo CT; Yamaguchi TP; Stappenbeck TS. 2012. Wnt5a potentiates TGF-beta signaling to promote colonic crypt regeneration after tissue injury. Science 338(6103):108-13. [PubMed: 22956684]  [MGI Ref ID J:187747]

Rudat C; Kispert A. 2012. Wt1 and epicardial fate mapping. Circ Res 111(2):165-9. [PubMed: 22693350]  [MGI Ref ID J:205035]

Smith CL; Baek ST; Sung CY; Tallquist MD. 2011. Epicardial-derived cell epithelial-to-mesenchymal transition and fate specification require PDGF receptor signaling. Circ Res 108(12):e15-26. [PubMed: 21512159]  [MGI Ref ID J:185693]

Tian X; Hu T; He L; Zhang H; Huang X; Poelmann RE; Liu W; Yang Z; Yan Y; Pu WT; Zhou B. 2013. Peritruncal coronary endothelial cells contribute to proximal coronary artery stems and their aortic orifices in the mouse heart. PLoS One 8(11):e80857. [PubMed: 24278332]  [MGI Ref ID J:209777]

Tian X; Hu T; Zhang H; He L; Huang X; Liu Q; Yu W; He L; Yang Z; Zhang Z; Zhong TP; Yang X; Yang Z; Yan Y; Baldini A; Sun Y; Lu J; Schwartz RJ; Evans SM; Gittenberger-de Groot AC; Red-Horse K; Zhou B. 2013. Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries. Cell Res 23(9):1075-90. [PubMed: 23797856]  [MGI Ref ID J:220957]

Trembley MA; Velasquez LS; de Mesy Bentley KL; Small EM. 2015. Myocardin-related transcription factors control the motility of epicardium-derived cells and the maturation of coronary vessels. Development 142(1):21-30. [PubMed: 25516967]  [MGI Ref ID J:217680]

Urbach A; Yermalovich A; Zhang J; Spina CS; Zhu H; Perez-Atayde AR; Shukrun R; Charlton J; Sebire N; Mifsud W; Dekel B; Pritchard-Jones K; Daley GQ. 2014. Lin28 sustains early renal progenitors and induces Wilms tumor. Genes Dev 28(9):971-82. [PubMed: 24732380]  [MGI Ref ID J:211179]

Zhao C; Guo H; Li J; Myint T; Pittman W; Yang L; Zhong W; Schwartz RJ; Schwarz JJ; Singer HA; Tallquist MD; Wu M. 2014. Numb family proteins are essential for cardiac morphogenesis and progenitor differentiation. Development 141(2):281-95. [PubMed: 24335256]  [MGI Ref ID J:206589]

Zhou B; Pu WT. 2012. Genetic Cre-loxP assessment of epicardial cell fate using Wt1-driven Cre alleles. Circ Res 111(11):e276-80. [PubMed: 23139287]  [MGI Ref ID J:205034]

Zhou B; von Gise A; Ma Q; Hu YW; Pu WT. 2010. Genetic fate mapping demonstrates contribution of epicardium-derived cells to the annulus fibrosis of the mammalian heart. Dev Biol 338(2):251-61. [PubMed: 20025864]  [MGI Ref ID J:156703]

von Gise A; Zhou B; Honor LB; Ma Q; Petryk A; Pu WT. 2011. WT1 regulates epicardial epithelial to mesenchymal transition through beta-catenin and retinoic acid signaling pathways. Dev Biol 356(2):421-31. [PubMed: 21663736]  [MGI Ref ID J:175466]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred together or to wildtype siblings. Homozygous mice die before birth.
Mating SystemHeterozygote x +/+ sibling         (Female x Male)   23-MAR-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $246.90Female or MaleHeterozygous for Wt1tm2(cre/ERT2)Wtp  
Price per Pair (US dollars $)Pair Genotype
$320.90Heterozygous for Wt1tm2(cre/ERT2)Wtp x Wild-type for Wt1tm2(cre/ERT2)Wtp  
$320.90Wild-type for Wt1tm2(cre/ERT2)Wtp x Heterozygous for Wt1tm2(cre/ERT2)Wtp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $321.00Female or MaleHeterozygous for Wt1tm2(cre/ERT2)Wtp  
Price per Pair (US dollars $)Pair Genotype
$417.20Heterozygous for Wt1tm2(cre/ERT2)Wtp x Wild-type for Wt1tm2(cre/ERT2)Wtp  
$417.20Wild-type for Wt1tm2(cre/ERT2)Wtp x Heterozygous for Wt1tm2(cre/ERT2)Wtp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

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Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Mice are subject to US Patent 6040430.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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