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| These ROSA26HABirA mice contain an HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene inserted into the Gt(ROSA)26Sor locus. When crossed with a strain containing an Avi-tagged sequence of interest, biotinylation of the target protein results. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Gene Trap; Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+5pN1
Generation DefinitionsDonating Investigator Dies Meijer, Erasmus University Medical Center Description
These ROSA26HABirA mice contain an HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene inserted into the Gt(ROSA)26Sor locus. When crossed with a strain containing an Avi-tagged sequence of interest, biotinylation of the target protein results. Biotinylation is highly specific, quantitative and allows purification of the target protein complexes. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. birA gene product (protein) is detected in all tissues by Western blot analysis. Secretory proteins are not efficiently biotinylated due to the location of the BirA protein in cytoplasm. This mutant mouse strain would be a useful tool for the isolation and purification of proteins and protein complexes from tissues.Development
An HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene was inserted into exon 3 of the rabbit beta-globin gene. The entire birA ORF, with the rabbit beta globin splice acceptor and transcription termination sequences were excised to generate a targeting vector that also contained a floxed PGK- puromycin selection cassette. This targeting vector was inserted into the first intron of the Gt(ROSA)26Sor gene. The construct was electroporated into 129P2/OlaHsd derived IB10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to FVB/N mice. The mice were intercrossed for 3 generations before being crossed to a Cre recombinase expressing strain (on an unknown genetic background) to remove the loxP site flanked PGK-puromycin selection cassette. A single loxP site remains. The mice no longer carry the Cre transgene. Upon arrival at The Jackson Laboratory the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (130 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(birA)Mejr/?
involves: 129P2/OlaHsd
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:100227)
This mouse can be used to support research in many areas including:
Cell Biology Research
Protein Processing
Research Tools
General Purpose
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(birA)Mejr | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Dies Meijer | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Rosa26BirA; | ||
| Mutation Made By | Dies Meijer, Erasmus University Medical Center | ||
| Strain of Origin | 129P2/OlaHsd | ||
| Expressed Gene | birA, biotin ligase), bacteria | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | The birA sequence was inserted into the third exon of the rabbit Beta-globin gene, and subsequently inserted into the first intron of the ROSA26 construct. Western blot of mutants demonstrated the expression of birA in all tissues tested, though levels of expression varied from tissue to tissue. [MGI Ref ID J:100227] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(birA)Mejr MCA,Separated MCA
Gt(ROSA)26Sortm1(birA)Mejr, Separated PCR
Gt(ROSA)26Sortm1(birA)Mejr, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Driegen S; Ferreira R; van Zon A; Strouboulis J; Jaegle M; Grosveld F; Philipsen S; Meijer D. 2005. A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Res 14(4):477-82. [PubMed: 16201414] [MGI Ref ID J:100227]
Additional References
Gt(ROSA)26Sortm1(birA)Mejr relatedCretney E; Xin A; Shi W; Minnich M; Masson F; Miasari M; Belz GT; Smyth GK; Busslinger M; Nutt SL; Kallies A. 2011. The transcription factors Blimp-1 and IRF4 jointly control the differentiation and function of effector regulatory T cells. Nat Immunol 12(4):304-11. [PubMed: 21378976] [MGI Ref ID J:170323]
He A; Shen X; Ma Q; Cao J; von Gise A; Zhou P; Wang G; Marquez VE; Orkin SH; Pu WT. 2012. PRC2 directly methylates GATA4 and represses its transcriptional activity. Genes Dev 26(1):37-42. [PubMed: 22215809] [MGI Ref ID J:179371]
McManus S; Ebert A; Salvagiotto G; Medvedovic J; Sun Q; Tamir I; Jaritz M; Tagoh H; Busslinger M. 2011. The transcription factor Pax5 regulates its target genes by recruiting chromatin-modifying proteins in committed B cells. EMBO J 30(12):2388-404. [PubMed: 21552207] [MGI Ref ID J:173158]
Ouyang W; Liao W; Luo CT; Yin N; Huse M; Kim MV; Peng M; Chan P; Ma Q; Mo Y; Meijer D; Zhao K; Rudensky AY; Atwal G; Zhang MQ; Li MO. 2012. Novel Foxo1-dependent transcriptional programs control T(reg) cell function. Nature 491(7425):554-9. [PubMed: 23135404] [MGI Ref ID J:189962]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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