Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator David R. Weaver,   Univ of Massachusetts Medical School

Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. Homozygotes die during late gestation and perinatally, with no homozygotes at weaning age. Homozygous embryos are smaller (approximately 30% less than control weight) than wild-type controls at embryonic day 18-19. A truncated non-functional gene product (protein), lacking residues 26 to 62, is detected by Western blot analysis and cotransfection assays of homozygotes. The truncated protein does not phosphorylate or degrade PER1, period homolog 1 (Drosophila), and PER2, period homolog 2 (Drosophila), proteins. After being kept in constant darkness for 10 days, heterozygotes exhibit a longer free-running period than wild-type controls.

Development
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then crossed to mice expressing Cre recombinase in the testis (Protamine-cre, on the C57BL6J background). Mice that no longer contained exon 2 or the Cre recombinase transgene were backcrossed to C57BL/6J for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed with C57BL/6J once to establish the colony.

Related Strains

Strains carrying other alleles of Csnk1d

010487

B6.129S4-Csnk1dtm1Drw/J

View Strains carrying other alleles of Csnk1d     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Csnk1d^tm1.1Drw/Csnk1d⁺

        B6.129S4-Csnk1d^tm1.1Drw

• behavior/neurological phenotype
• prolonged circadian period
  • when kept in constant darkness for 10 days, the free-running period is longer than in wild-type mice   (MGI Ref ID J:150148)
  • however, the amplitude of rhythmicity in the suprachiasmatic nuclei is normal   (MGI Ref ID J:150148)

Csnk1d^tm1.1Drw/Csnk1d^tm1.1Drw

        B6.129S4-Csnk1d^tm1.1Drw

• mortality/aging
• complete preweaning lethality
  • no mice are present at weaning   (MGI Ref ID J:150148)
• partial lethality throughout fetal growth and development
  • fewer than expected mice are present at E18 to E19   (MGI Ref ID J:150148)
• growth/size phenotype
• decreased fetal weight
  • by 30% at E18 and E19   (MGI Ref ID J:150148)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Perinatal Lethality
      Homozygous

Neurobiology Research
Circadian Rhythms

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Csnk1dtm1.1Drw
Allele Name		targeted mutation 1.1, David R Weaver
Allele Type		Targeted (knock-out)
Common Name(s)		CK1deltadelta2;
Mutation Made By		David Weaver,   Univ of Massachusetts Medical School
Strain of Origin		129S4/SvJae
Gene Symbol and Name		Csnk1d, casein kinase 1, delta
Chromosome		11
Gene Common Name(s)		1200006A05Rik; AA409348; ASPS; CKIdelta; FASPS2; HCKID; RIKEN cDNA 1200006A05 gene; expressed sequence AA409348;
Molecular Note		Germ line, cre mediated recombination removed exon 2. The absence of exon 2 leads to the deletion of residues 26 and 62 and produces a non-functional truncated protein. [MGI Ref ID J:150148]

Genotyping

Genotyping Information

Genotyping Protocols

Csnk1d^tm1.1Drw, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Etchegaray JP; Machida KK; Noton E; Constance CM; Dallmann R; Di Napoli MN; DeBruyne JP; Lambert CM; Yu EA; Reppert SM; Weaver DR. 2009. Casein kinase 1 delta regulates the pace of the mammalian circadian clock. Mol Cell Biol 29(14):3853-66. [PubMed: 19414593]  [MGI Ref ID J:150148]

Additional References

Csnk1d^tm1.1Drw related

Etchegaray JP; Yu EA; Indic P; Dallmann R; Weaver DR. 2010. Casein kinase 1 delta (CK1delta) regulates period length of the mouse suprachiasmatic circadian clock in vitro. PLoS One 5(4):e10303. [PubMed: 20421981]  [MGI Ref ID J:160138]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes die during late gestation and perinatally, with no homozygotes at weaning age.

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Pricing, Supply Level & Notes, Controls

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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