Strain Name:

B6.129S4-Clocktm1.1Rep/J

Stock Number:

010925

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Mice that are homozygous for this targeted mutation, when kept in constant darkness, exhibit a shorter circadian period than wild-type controls. This mutant mouse strain may be useful in studies of circadian rhythm and behavior.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S4-Clocktm1.1Drw/J    (Changed: 11-FEB-10 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator David R. Weaver,   Univ of Massachusetts Medical School

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by immunohistochemical and immunoprecipitation analysis of SCN (suprachiasma nucleus). Homozygotes exhibit a shorter circadian period of approximately 20 minutes, when kept in constant darkness. While under 12 hr light/12 hr dark (LD) cycle, homozygotes become active approximately 2 hours before the start of the dark cycle, indicating altered response to light cues. A four hour extended light period preceding constant darkness does not result in the expected activity rhythm phase delay. Re-entrainment with LD cycle followed by a 4 hour light pulse in the dark cycle does not cause activity rhythm phase advance.

Development
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 5 of the targeted gene, and another loxP site was inserted downstream of exon 6. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then crossed to transgenic mice expressing Cre recombinase in the male germ line, to remove the floxed exons (Protamine-cre, on the C57BL6J background). Mice that no longer contained exons 5-6 or the Cre recombinase transgene were backcrossed to C57BL/6J for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed with C57BL/6J once to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Clock
010490   B6.129S4-Clocktm1Rep/J
016167   B6.Cg-ClockGt(P007F12)Wrst/JtJ
016182   B6.Cg-Clocktm1Jt/J
008277   B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J
016175   C.B6-Clockm1Jt/J
002923   C57BL/6J-Clockm1Jt/J
008278   C57BL/6J-Tg(tetO-Clock)1Jt/J
View Strains carrying other alleles of Clock     (7 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Clocktm1.1Rep/Clocktm1.1Rep

        involves: 129S4/SvJae * C57BL/6J
  • behavior/neurological phenotype
  • abnormal circadian phase
    • after light-dark entrainment for several weeks (L/D - 12/12 hours) followed by an extension of the light period by 4 hours, wild-type mice and heterozygotes exhibit a phase delay of ~1.6-1.9 hours whereas mutants don't show any delay   (MGI Ref ID J:109634)
    • advanced circadian phase
      • mutants become active ~ 2 hours before lights-off (start of dark cycle) while wild-type mice show activity at onset of dark period   (MGI Ref ID J:109634)
      • after LD entrainment for 3 weeks, exposure to a light pulse late in the dark phase followed by release into total darkness results in a phase advance of 6.8 hours compared to 1.8-2 hours exhibited by wild-type and heterozygotes in the same paradigm   (MGI Ref ID J:109634)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Circadian Rhythms

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Clocktm1.1Rep
Allele Name targeted mutation 1.1, Steven M Reppert
Allele Type Targeted (knock-out)
Common Name(s) Clockdelta5-6; Clocktm1.1Drw;
Mutation Made By Steven Reppert,   University of Massachusetts Medical Scho
Strain of Origin129S4/SvJae
Gene Symbol and Name Clock, circadian locomotor output cycles kaput
Chromosome 5
Gene Common Name(s) 5330400M04Rik; KAT13D; RIKEN cDNA 5330400M04 gene; bHLHe8; mKIAA0334;
Molecular Note Cre mediated recombination resulted in the excision of exons 5 and 6. Immunohistochemistry confirmed absence of protein in mutants. [MGI Ref ID J:109634]

Genotyping

Genotyping Information

Genotyping Protocols

Clocktm1.1Rep, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Debruyne JP; Noton E; Lambert CM; Maywood ES; Weaver DR; Reppert SM. 2006. A clock shock: mouse CLOCK is not required for circadian oscillator function. Neuron 50(3):465-77. [PubMed: 16675400]  [MGI Ref ID J:109634]

Additional References

Clocktm1.1Rep related

Bellet MM; Deriu E; Liu JZ; Grimaldi B; Blaschitz C; Zeller M; Edwards RA; Sahar S; Dandekar S; Baldi P; George MD; Raffatellu M; Sassone-Corsi P. 2013. Circadian clock regulates the host response to Salmonella. Proc Natl Acad Sci U S A 110(24):9897-902. [PubMed: 23716692]  [MGI Ref ID J:197391]

Cretenet G; Le Clech M; Gachon F. 2010. Circadian clock-coordinated 12 Hr period rhythmic activation of the IRE1alpha pathway controls lipid metabolism in mouse liver. Cell Metab 11(1):47-57. [PubMed: 20074527]  [MGI Ref ID J:157002]

DeBruyne JP; Weaver DR; Reppert SM. 2007. CLOCK and NPAS2 have overlapping roles in the suprachiasmatic circadian clock. Nat Neurosci 10(5):543-5. [PubMed: 17417633]  [MGI Ref ID J:121658]

DeBruyne JP; Weaver DR; Reppert SM. 2007. Peripheral circadian oscillators require CLOCK. Curr Biol 17(14):R538-9. [PubMed: 17637349]  [MGI Ref ID J:123965]

Eckel-Mahan KL; Patel VR; Mohney RP; Vignola KS; Baldi P; Sassone-Corsi P. 2012. Coordination of the transcriptome and metabolome by the circadian clock. Proc Natl Acad Sci U S A 109(14):5541-6. [PubMed: 22431615]  [MGI Ref ID J:182655]

LeSauter J; Lambert CM; Robotham MR; Model Z; Silver R; Weaver DR. 2012. Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus. PLoS One 7(4):e35938. [PubMed: 22558277]  [MGI Ref ID J:187275]

Masri S; Patel VR; Eckel-Mahan KL; Peleg S; Forne I; Ladurner AG; Baldi P; Imhof A; Sassone-Corsi P. 2013. Circadian acetylome reveals regulation of mitochondrial metabolic pathways. Proc Natl Acad Sci U S A 110(9):3339-44. [PubMed: 23341599]  [MGI Ref ID J:193829]

Peruquetti RL; de Mateo S; Sassone-Corsi P. 2012. Circadian proteins CLOCK and BMAL1 in the chromatoid body, a RNA processing granule of male germ cells. PLoS One 7(8):e42695. [PubMed: 22900038]  [MGI Ref ID J:189932]

Spengler ML; Kuropatwinski KK; Comas M; Gasparian AV; Fedtsova N; Gleiberman AS; Gitlin II; Artemicheva NM; Deluca KA; Gudkov AV; Antoch MP. 2012. Core circadian protein CLOCK is a positive regulator of NF-kappaB-mediated transcription. Proc Natl Acad Sci U S A 109(37):E2457-65. [PubMed: 22895791]  [MGI Ref ID J:189804]

Zuber AM; Centeno G; Pradervand S; Nikolaeva S; Maquelin L; Cardinaux L; Bonny O; Firsov D. 2009. Molecular clock is involved in predictive circadian adjustment of renal function. Proc Natl Acad Sci U S A 106(38):16523-8. [PubMed: 19805330]  [MGI Ref ID J:153224]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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