| |||||||||||||||
| This transgenic line expresses hepsin and hrGFP in the ventral lobe of the prostate and exhibits disruptions in the basement membrane in mature males. This mutant mouse strain may be useful in studies of prostate carcinogenesis. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(Pbsn-Hpn)DVv/J (Changed: 23-SEP-09 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Valeri Vasioukhin, Fred Hutchinson Cancer Research Center Description
Hemizygous mice are viable and express mouse hepsin (Hpn) and humanized Renilla Green Fluorescent Protein under the control of the rat probasin promoter. Tissues in the transgenic animals show GFP fluorescence patterns consistent with the hepsin transgene expression, specifically in the ventral lobe of the prostate. Immunofluorescence staining with antibodies specific to basement membrane, histological and electron microscopical examination suggest that the basement membrane in the prostate gland is severely compromised. This mutant mouse strain may be useful in studies of prostate carcinogenesis.Development
A transgenic construct containing a modified rat probasin (Pbsn) promoter, beta globin intron, mouse hepsin (Hpn) full length cDNA , and an IRES-humanized Renilla (hr)GFP cassette was injected into C57BL/6 X CBA fertilized embryos. Founder line D was subsequently established. This line has been backcrossed to C57BL/6 for at least 6 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
010929 129S.Cg-Tg(Pbsn-ERG*)1Vv/J 008878 FVB-Tg(Pbsn-Ar*E231G)7353Ng/J 008874 FVB-Tg(Pbsn-IGF1*)5305Ng/J 003739 FVB.Cg-Tg(Pbsn-Cdc37)1Stp/J 003689 STOCK Tg(Pbsn-Cdc37)1Stp/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Pbsn-Hpn,-GFP)DVv/0
involves: C57BL/6J * CBA
- reproductive system phenotype
- abnormal prostate gland morphology (MGI Ref ID J:92562)
- 75% of transgenic mice exhibit separation between epithelial and stromal cell layers in contrast to controls
- small areas of separation is observed in 14-week old mice
- ultrastructural analysis of prostatic basement membranes shows microblisters separating the epithelial and stromal cell compartments; disruption is at the level of the basement membrane with compromised adhesion between collagen fibers
- endocrine/exocrine gland phenotype
- abnormal prostate gland morphology (MGI Ref ID J:92562)
- 75% of transgenic mice exhibit separation between epithelial and stromal cell layers in contrast to controls
- small areas of separation is observed in 14-week old mice
- ultrastructural analysis of prostatic basement membranes shows microblisters separating the epithelial and stromal cell compartments; disruption is at the level of the basement membrane with compromised adhesion between collagen fibers
This mouse can be used to support research in many areas including:
GFP relatedCancer Research
Increased Tumor Incidence
Prostate Tumors
Reproductive Biology Research
Prostate Tumors
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Pbsn-Hpn,-GFP)DVv | ||
|---|---|---|---|
| Allele Name | transgene insertion D, Valeri Vasioukhin | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | PB-hepsin Line D; | ||
| Mutation Made By | Valeri Vasioukhin, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | C57BL/6 x CBA | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Hpn, hepsin, mouse, laboratory | ||
| Promoter | Pbsn, probasin, rat | ||
| Molecular Note | A transgenic construct containing a modified rat probasin (Pbsn) promoter, beta globin intron, mouse hepsin (Hpn) full length cDNA , and an IRES-humanized Renilla (hr) GFP cassette was injected into C57BL/6 x CBA fertilized embryos. Founder line D was subsequently established. [MGI Ref ID J:92562] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Klezovitch O; Chevillet J; Mirosevich J; Roberts RL; Matusik RJ; Vasioukhin V. 2004. Hepsin promotes prostate cancer progression and metastasis. Cancer Cell 6(2):185-95. [PubMed: 15324701] [MGI Ref ID J:92562]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry While maintaining a live colony, these mice are bred as hemizygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.