Strain Name:

NODCaj.129S2(B6)-H2-Ab1tm1Doi/LwnJ

Stock Number:

010966

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Common Names: NOD.Ab null;     NOD.Ab0;    
NOD. H2-Ab1 deficient mice are diabetes resistant and exhibit a dramatic decrease of CD4+ T cells when compared to NOD/ShiLtJ. This model may be useful to facilitate the engraftment of immune cells in adoptive transfer experiments and to understand the role of MHC class II in autoimmunity.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NODShiDvs.129S2(B6)-H2-Ab1tm1Gru/LwnJ    (Changed: 29-OCT-09 )
NODShiDvs.129S2-H2-Ab1tm1Gru/LwnJ    (Changed: 29-SEP-09 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain NOD/CaJ
Donor Strain 129S2
GenerationN12+F22pN1
Generation Definitions
 
Donating Investigator Li Wen,   Yale School of Medicine

Description
Mice homozygous for MHC class II gene, H2-Ab1 targeted mutation, commonly referred to as NOD-Ab0, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. NOD. Ab0 mice are diabetes resistant. MHC class II gene products (mRNA or protein) are not detected. FACS analysis of peripheral blood lymphocytes (PBL's) indicates a dramatic decrease is observed in the number of CD4+ T cells when compared to NOD/ShiLtJ (Stock No. 001976). In addition, FACS of PBL's verifies the MHC class I variant Kb (129S2 derived) is in linkage to the Ab0 mutation. SNP and Mit marker assisted analysis indicates the Chromosome 17 markers D17Mit27 through D17Mit176 to be of 129S2 origin and is about 29 mb long. No other non-NOD contaminating regions were identified. In 2008, the T1DR received this stock at N11F?. This strain was mated to NOD/LtJ (Stock No. 001976) once prior to breeding to homozygosity. This model may be useful to facilitate the engraftment of immune cells in adoptive transfer experiments and to understand the role of MHC class II in autoimmunity.

Development
A construct containing a neomycin expression cassette was inserted into the second exon of H2-Ab1 gene. The construct was transfected into D3, 129S2/SvPas, embryonic stem (ES) cells, which carries a deletion in the promoter region of the H2-Ea. These ES cells were injected into C57BL/6 blastocysts. Progeny of chimeric mice were mated with C57BL/6. The H2-Ab1tm1Doi mutation was backcrossed for 10 generations to NOD/CaJ prior to making homozygous. In 2009, SNP and Mit marker testing performed by the T1DR indicate that there is no contaminating genome outside of the congenic region on Chr. 17.

Control Information

  Control
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   H2-Ab1tm1Doi allele
004606   NOD.Cg-Prkdcscid H2-Ab1tm1Doi Tg(HLA-DQA1,HLA-DQB1)1Dv/SzJ
005589   NOD.Cg-Prkdcscid H2-Ab1tm1Doi/SzJ
View Strains carrying   H2-Ab1tm1Doi     (2 strains)

View Strains carrying other alleles of H2-Ab1     (13 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

H2-Ab1tm1Gru/H2-Ab1tm1Gru

        NOD.129S2-H2-Ab1tm1Gru
  • immune system phenotype
  • *normal* immune system phenotype
    • NOD mice homozygous for this targeted mutation alone show no indication of inflammation at any age   (MGI Ref ID J:86540)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • NOD mice homozygous for this targeted mutation alone show no indication of cardiac enlargement or inflammation at any age   (MGI Ref ID J:86540)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

H2-Ab1tm1Doi/H2-Ab1+

        involves: 129S2/SvPas * C57BL/6 * NOD/Lt
  • endocrine/exocrine gland phenotype
  • insulitis
    • heterozygous mice show insulitis less frequently and less severely than homozygotes   (MGI Ref ID J:93557)
  • immune system phenotype
  • insulitis
    • heterozygous mice show insulitis less frequently and less severely than homozygotes   (MGI Ref ID J:93557)

H2-Ab1tm1Doi/H2-Ab1tm1Doi

        involves: 129S2/SvPas * C57BL/6 * NOD/Lt
  • endocrine/exocrine gland phenotype
  • insulitis
    • 10-11 week old mice of the fourth backcross generation to NOD/Lt mice are completely free of insulitis compared to NOD controls which develop insulitis by 6-8 weeks   (MGI Ref ID J:93557)
  • immune system phenotype
  • insulitis
    • 10-11 week old mice of the fourth backcross generation to NOD/Lt mice are completely free of insulitis compared to NOD controls which develop insulitis by 6-8 weeks   (MGI Ref ID J:93557)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
      resistant

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
      MHC class II deficient

Research Tools
Immunology, Inflammation and Autoimmunity Research
      MHC class I deficiency
      T cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol H2-Ab1tm1Doi
Allele Name targeted mutation 1, Christophe Benoist and Diane Mathis
Allele Type Targeted (Null/Knockout)
Common Name(s) A beta0; Abbeta-; AB0; Abeta-; Class II0; H2-Ab1tm1Dim; H2-Ab1tm1Doi; II0; MHC class II-; MHC-II-k.o.; MHCII-;
Mutation Made By Christophe Benoist,   Joslin Diabetes Center
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name H2-Ab1, histocompatibility 2, class II antigen A, beta 1
Chromosome 17
Gene Common Name(s) A beta; AI845868; Abeta; Bb; CELIAC1; H-2Ab; H2-Ab; HLA-DQB; I-Ab; I-Abeta; I-region-associated antigen 2; IAb; IDDM1; Ia-2; Ia2; RT1.B; Rmcs1; expressed sequence AI845868; histocompatibility 2, class II antigen A, beta; response to metastatic cancers 1;
Molecular Note The second exon was disrupted by the insertion of a neomycin resistance gene. In addition, the ES cell line used was derived from the 129S2/SvPas strain, which carries a deletion in the promoter region of H2-Ea. Consequently, these MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins. [MGI Ref ID J:65736]

Genotyping

Genotyping Information

Genotyping Protocols

H2-Ab1tm1Gru/H2-Ab1tm1Doi, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

H2-Ab1tm1Doi related

Abraham RS; Wen L; Marietta EV; David CS. 2001. Type 1 diabetes-predisposing MHC alleles influence the selection of glutamic acid decarboxylase (GAD) 65-specific T cells in a transgenic model. J Immunol 166(2):1370-9. [PubMed: 11145722]  [MGI Ref ID J:66858]

Aguado E; Richelme S; Nunez-Cruz S; Miazek A; Mura AM; Richelme M; Guo XJ; Sainty D; He HT; Malissen B; Malissen M. 2002. Induction of T helper type 2 immunity by a point mutation in the LAT adaptor. Science 296(5575):2036-40. [PubMed: 12065839]  [MGI Ref ID J:77098]

Aichinger M; Wu C; Nedjic J; Klein L. 2013. Macroautophagy substrates are loaded onto MHC class II of medullary thymic epithelial cells for central tolerance. J Exp Med 210(2):287-300. [PubMed: 23382543]  [MGI Ref ID J:196474]

Bardos T; Zhang J; Mikecz K; David CS; Glant TT. 2002. Mice lacking endogenous major histocompatibility complex class II develop arthritis resembling psoriatic arthritis at an advanced age. Arthritis Rheum 46(9):2465-75. [PubMed: 12355495]  [MGI Ref ID J:134117]

Barr TA; Brown S; Mastroeni P; Gray D. 2010. TLR and B cell receptor signals to B cells differentially program primary and memory Th1 responses to Salmonella enterica. J Immunol 185(5):2783-9. [PubMed: 20675594]  [MGI Ref ID J:163256]

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Behrens GM; Li M; Davey GM; Allison J; Flavell RA; Carbone FR; Heath WR. 2004. Helper requirements for generation of effector CTL to islet beta cell antigens. J Immunol 172(9):5420-6. [PubMed: 15100283]  [MGI Ref ID J:89639]

Benhenda S; Ducroux A; Riviere L; Sobhian B; Ward MD; Dion S; Hantz O; Protzer U; Michel ML; Benkirane M; Semmes OJ; Buendia MA; Neuveut C. 2013. Methyltransferase PRMT1 is a binding partner of HBx and a negative regulator of hepatitis B virus transcription. J Virol 87(8):4360-71. [PubMed: 23388725]  [MGI Ref ID J:197216]

Bihl F; Pecheur J; Breart B; Poupon G; Cazareth J; Julia V; Glaichenhaus N; Braud VM. 2010. Primed Antigen-Specific CD4+ T Cells Are Required for NK Cell Activation In Vivo upon Leishmania major Infection. J Immunol 185(4):2174-81. [PubMed: 20624944]  [MGI Ref ID J:162384]

Black KE; Murray JA; David CS. 2002. HLA-DQ Determines the Response to Exogenous Wheat Proteins: A Model of Gluten Sensitivity in Transgenic Knockout Mice. J Immunol 169(10):5595-600. [PubMed: 12421937]  [MGI Ref ID J:80059]

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Bommhardt U; Basson MA; Krummrei U; Zamoyska R. 1999. Activation of the extracellular signal-related kinase/mitogen-activated protein kinase pathway discriminates CD4 versus CD8 lineage commitment in the thymus. J Immunol 163(2):715-22. [PubMed: 10395662]  [MGI Ref ID J:56161]

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Brown DR; Swier K; Moskowitz NH; Naujokas MF; Locksley RM; Reiner SL. 1997. T helper subset differentiation in the absence of invariant chain. J Exp Med 185(1):31-41. [PubMed: 8996239]  [MGI Ref ID J:111104]

Brown NK; McCormick DJ; Brusic V; David CS; Kong YC. 2008. A novel H2A-E+ transgenic model susceptible to human but not mouse thyroglobulin-induced autoimmune thyroiditis: identification of mouse pathogenic epitopes. Cell Immunol 251(1):1-7. [PubMed: 18489063]  [MGI Ref ID J:136313]

Broxmeyer HE; Cooper S; Hangoc G; Chang CH. 2006. Class II transactivator-mediated regulation of major histocompatibility complex class II antigen expression is important for hematopoietic progenitor cell suppression by chemokines and iron-binding proteins. Exp Hematol 34(8):1078-84. [PubMed: 16863914]  [MGI Ref ID J:111903]

Buhlmann JE; Foy TM; Aruffo A; Crassi KM; Ledbetter JA; Green WR; Xu JC; Shultz LD; Roopesian D; Flavell RA; et al.. 1995. In the absence of a CD40 signal, B cells are tolerogenic. Immunity 2(6):645-53. [PubMed: 7540943]  [MGI Ref ID J:189416]

Buning J; Schmitz M; Repenning B; Ludwig D; Schmidt MA; Strobel S; Zimmer KP. 2005. Interferon-gamma mediates antigen trafficking to MHC class II-positive late endosomes of enterocytes. Eur J Immunol 35(3):831-42. [PubMed: 15688349]  [MGI Ref ID J:96737]

Chan S; Correia-Neves M; Dierich A; Benoist C; Mathis D. 1998. Visualization of CD4/CD8 T cell commitment. J Exp Med 188(12):2321-33. [PubMed: 9858518]  [MGI Ref ID J:113263]

Chan SH; Cosgrove D; Waltzinger C; Benoist C; Mathis D. 1993. Another view of the selective model of thymocyte selection. Cell 73(2):225-36. [PubMed: 8097430]  [MGI Ref ID J:111130]

Chapoval SP; Iijima K; Marietta EV; Smart MK; Chapoval AI; Andrews AG; David CS. 2002. Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene. J Immunol 168(2):890-9. [PubMed: 11777987]  [MGI Ref ID J:73744]

Chapoval SP; Marietta EV; Smart MK; David CS. 2001. Requirements for allergen-induced airway inflammation and hyperreactivity in CD4-deficient and CD4-sufficient HLA-DQ transgenic mice. J Allergy Clin Immunol 108(5):764-71. [PubMed: 11692102]  [MGI Ref ID J:106479]

Chapoval SP; Nabozny GH; Marietta EV; Raymond EL; Krco CJ; Andrews AG; David CS. 1999. Short ragweed allergen induces eosinophilic lung disease in HLA-DQ transgenic mice. J Clin Invest 103(12):1707-17. [PubMed: 10377177]  [MGI Ref ID J:118972]

Chen Z; Dudek N; Wijburg O; Strugnell R; Brown L; Deliyannis G; Jackson D; Koentgen F; Gordon T; McCluskey J. 2002. A 320-kilobase artificial chromosome encoding the human HLA DR3-DQ2 MHC haplotype confers HLA restriction in transgenic mice. J Immunol 168(6):3050-6. [PubMed: 11884478]  [MGI Ref ID J:126283]

Cheng S; Smart M; Hanson J; David CS. 2003. Characterization of HLA DR2 and DQ8 transgenic mouse with a new engineered mouse class II deletion, which lacks all endogenous class II genes. J Autoimmun 21(3):195-9. [PubMed: 14599844]  [MGI Ref ID J:86434]

Choo DK; Murali-Krishna K; Anita R; Ahmed R. 2010. Homeostatic turnover of virus-specific memory CD8 T cells occurs stochastically and is independent of CD4 T cell help. J Immunol 185(6):3436-44. [PubMed: 20733203]  [MGI Ref ID J:163530]

Chowdhary VR; Tilahun AY; Clark CR; Grande JP; Rajagopalan G. 2012. Chronic exposure to staphylococcal superantigen elicits a systemic inflammatory disease mimicking lupus. J Immunol 189(4):2054-62. [PubMed: 22798666]  [MGI Ref ID J:189749]

Cigel F; Batchelder J; Burns JM Jr; Yanez D; van der Heyde H; Manning DD; Weidanz WP. 2003. Immunity to blood-stage murine malarial parasites is MHC class II dependent. Immunol Lett 89(2-3):243-9. [PubMed: 14556985]  [MGI Ref ID J:110978]

Cosgrove D; Gray D; Dierich A; Kaufman J; Lemeur M; Benoist C; Mathis D. 1991. Mice lacking MHC class II molecules. Cell 66(5):1051-66. [PubMed: 1909605]  [MGI Ref ID J:65736]

Crawford A; Macleod M; Schumacher T; Corlett L; Gray D. 2006. Primary T cell expansion and differentiation in vivo requires antigen presentation by B cells. J Immunol 176(6):3498-506. [PubMed: 16517718]  [MGI Ref ID J:129511]

Dautigny N; Le Campion A; Lucas B. 1999. Timing and casting for actors of thymic negative selection. J Immunol 162(3):1294-302. [PubMed: 9973382]  [MGI Ref ID J:124433]

Desvignes C; Bour H; Nicolas JF; Kaiserlian D. 1996. Lack of oral tolerance but oral priming for contact sensitivity to dinitrofluorobenzene in major histocompatibility complex class II-deficient mice and in CD4+ T cell-depleted mice. Eur J Immunol 26(8):1756-61. [PubMed: 8765017]  [MGI Ref ID J:34592]

Dubois B; Chapat L; Goubier A; Papiernik M; Nicolas JF; Kaiserlian D. 2003. Innate CD4+CD25+ regulatory T cells are required for oral tolerance and inhibition of CD8+ T cells mediating skin inflammation. Blood 102(9):3295-301. [PubMed: 12855551]  [MGI Ref ID J:115675]

Emoto M; Mittrucker HW; Schmits R; Mak TW; Kaufmann SH. 1999. Critical role of leukocyte function-associated antigen-1 in liver accumulation of CD4+NKT cells. J Immunol 162(9):5094-8. [PubMed: 10227978]  [MGI Ref ID J:111009]

Fiette L; Brahic M; Pena-Rossi C. 1996. Infection of class II-deficient mice by the DA strain of Theiler's virus. J Virol 70(7):4811-5. [PubMed: 8676513]  [MGI Ref ID J:33649]

Fillatreau S; Gray D. 2003. T cell accumulation in B cell follicles is regulated by dendritic cells and is independent of B cell activation. J Exp Med 197(2):195-206. [PubMed: 12538659]  [MGI Ref ID J:124822]

Flynn JC; Gilbert JA; Meroueh C; Snower DP; David CS; Kong YC; Banga JP. 2007. Chronic exposure in vivo to thyrotropin receptor stimulating monoclonal antibodies sustains high thyroxine levels and thyroid hyperplasia in thyroid autoimmunity-prone HLA-DRB1*0301 transgenic mice. Immunology 122(2):261-7. [PubMed: 17535305]  [MGI Ref ID J:125652]

Flynn JC; Wan Q; Panos JC; McCormick DJ; Giraldo AA; David CS; Kong YC. 2002. Coexpression of susceptible and resistant HLA class II transgenes in murine experimental autoimmune thyroiditis: DQ8 molecules downregulate DR3-mediated thyroiditis. J Autoimmun 18(3):213-20. [PubMed: 12126634]  [MGI Ref ID J:127841]

Foucras G; Coudert JD; Coureau C; Guery JC. 2000. Dendritic cells prime in vivo alloreactive CD4 T lymphocytes toward type 2 cytokine- and TGF-beta-producing cells in the absence of CD8 T cell activation. J Immunol 165(9):4994-5003. [PubMed: 11046027]  [MGI Ref ID J:118923]

Freland S; Chambers BJ; Andersson M; Van Kaer L; Ljunggren HG. 1998. Rejection of allogeneic and syngeneic but not MHC class I-deficient tumor grafts by MHC class I-deficient mice. J Immunol 160(2):572-9. [PubMed: 9551890]  [MGI Ref ID J:45189]

Fukui Y; Oono T; Cabaniols JP; Nakao K; Hirokawa K; Inayoshi A; Sanui T; Kanellopoulos J; Iwata E; Noda M; Katsuki M; Kourilsky P; Sasazuki T. 2000. Diversity of T cell repertoire shaped by a single peptide ligand is critically affected by its amino acid residue at a T cell receptor contact. Proc Natl Acad Sci U S A 97(25):13760-5. [PubMed: 11087837]  [MGI Ref ID J:125474]

Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. [PubMed: 19433445]  [MGI Ref ID J:150146]

Gaszewska-Mastalarz A; Muranski P; Chmielowski B; Kraj P; Ignatowicz L. 2000. Altered selection of CD4+ T cells by class II MHC bound with dominant and low abundance self-peptides. J Immunol 165(11):6099-106. [PubMed: 11086042]  [MGI Ref ID J:118564]

Giannoni F; Shea A; Inglis C; Lee LN; Sarawar SR. 2008. CD40 engagement on dendritic cells, but not on B or T cells, is required for long-term control of murine gammaherpesvirus 68. J Virol 82(22):11016-22. [PubMed: 18768977]  [MGI Ref ID J:142958]

Goold HD; Escors D; Conlan TJ; Chakraverty R; Bennett CL. 2011. Conventional dendritic cells are required for the activation of helper-dependent CD8 T cell responses to a model antigen after cutaneous vaccination with lentiviral vectors. J Immunol 186(8):4565-72. [PubMed: 21389256]  [MGI Ref ID J:172461]

Graf D; Nethisinghe S; Palmer DB; Fisher AG; Merkenschlager M. 2002. The developmentally regulated expression of Twisted gastrulation reveals a role for bone morphogenetic proteins in the control of T cell development. J Exp Med 196(2):163-71. [PubMed: 12119341]  [MGI Ref ID J:120461]

Guo J; McLachlan SM; Pichurin PN; Chen CR; Pham N; Aliesky HA; David CS; Rapoport B. 2005. Relationship between thyroid peroxidase T cell epitope restriction and antibody recognition of the autoantibody immunodominant region in human leukocyte antigen DR3 transgenic mice. Endocrinology 146(11):4961-7. [PubMed: 16081633]  [MGI Ref ID J:129450]

Hanano R; Kaufmann SH. 1999. Effect on parasite eradication of Pneumocystis carinii-specific antibodies produced in the presence or absence of CD4(+) alphabeta T lymphocytes. Eur J Immunol 29(8):2464-75. [PubMed: 10458760]  [MGI Ref ID J:115322]

Hess J; Ladel C; Miko D; Kaufmann SH. 1996. Salmonella typhimurium aroA- infection in gene-targeted immunodeficient mice: major role of CD4+ TCR-alpha beta cells and IFN-gamma in bacterial clearance independent of intracellular location. J Immunol 156(9):3321-6. [PubMed: 8617956]  [MGI Ref ID J:32521]

Hikosaka Y; Nitta T; Ohigashi I; Yano K; Ishimaru N; Hayashi Y; Matsumoto M; Matsuo K; Penninger JM; Takayanagi H; Yokota Y; Yamada H; Yoshikai Y; Inoue J; Akiyama T; Takahama Y. 2008. The cytokine RANKL produced by positively selected thymocytes fosters medullary thymic epithelial cells that express autoimmune regulator. Immunity 29(3):438-50. [PubMed: 18799150]  [MGI Ref ID J:139648]

Huang GJ; Smith AL; Gray DH; Cosgrove C; Singer BH; Edwards A; Sim S; Parent JM; Johnsen A; Mott R; Mathis D; Klenerman P; Benoist C; Flint J. 2010. A genetic and functional relationship between T cells and cellular proliferation in the adult hippocampus. PLoS Biol 8(12):e1000561. [PubMed: 21179499]  [MGI Ref ID J:170591]

Huber SA; Sakkinen P; David C; Newell MK; Tracy RP. 2001. T helper-cell phenotype regulates atherosclerosis in mice under conditions of mild hypercholesterolemia. Circulation 103(21):2610-6. [PubMed: 11382732]  [MGI Ref ID J:133189]

Johnson S; Zhan Y; Sutherland RM; Mount AM; Bedoui S; Brady JL; Carrington EM; Brown LE; Belz GT; Heath WR; Lew AM. 2009. Selected Toll-like receptor ligands and viruses promote helper-independent cytotoxic T cell priming by upregulating CD40L on dendritic cells. Immunity 30(2):218-27. [PubMed: 19200758]  [MGI Ref ID J:146462]

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Kadioglu A; Coward W; Colston MJ; Hewitt CR; Andrew PW. 2004. CD4-T-lymphocyte interactions with pneumolysin and pneumococci suggest a crucial protective role in the host response to pneumococcal infection. Infect Immun 72(5):2689-97. [PubMed: 15102777]  [MGI Ref ID J:89530]

Katz J; Benoist C; Mathis D. 1993. Major histocompatibility complex class I molecules are required for the development of insulitis in non-obese diabetic mice. Eur J Immunol 23(12):3358-60. [PubMed: 8258349]  [MGI Ref ID J:93557]

Kawano M; Nakayama M; Aoshima Y; Nakamura K; Ono M; Nishiya T; Nakamura S; Takeda Y; Dobashi A; Takahashi A; Endo M; Ito A; Ueda K; Sato N; Higuchi S; Kondo T; Hashimoto S; Watanabe M; Watanabe M; Takahashi T; Sasaki K; Nakamura M; Sasazuki T; Narushima T; Suzuki R; Ogasawara K. 2014. NKG2D(+) IFN-gamma(+) CD8(+) T cells are responsible for palladium allergy. PLoS One 9(2):e86810. [PubMed: 24533050]  [MGI Ref ID J:212941]

Kehren J; Desvignes C; Krasteva M; Ducluzeau MT; Assossou O; Horand F; Hahne M; Kagi D; Kaiserlian D; Nicolas JF. 1999. Cytotoxicity is mandatory for CD8(+) T cell-mediated contact hypersensitivity. J Exp Med 189(5):779-86. [PubMed: 10049941]  [MGI Ref ID J:112018]

Komaniwa S; Hayashi H; Kawamoto H; Sato SB; Ikawa T; Katsura Y; Udaka K. 2009. Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage. Eur J Immunol 39(1):96-112. [PubMed: 19089815]  [MGI Ref ID J:143734]

Kraj P; Pacholczyk R; Ignatowicz L. 2001. Alpha beta TCRs differ in the degree of their specificity for the positively selecting MHC/peptide ligand. J Immunol 166(4):2251-9. [PubMed: 11160279]  [MGI Ref ID J:126970]

Krajina T; Leithauser F; Reimann J. 2004. MHC class II-independent CD25+ CD4+ CD8alpha beta+ alpha beta T cells attenuate CD4+ T cell-induced transfer colitis. Eur J Immunol 34(3):705-14. [PubMed: 14991600]  [MGI Ref ID J:88382]

Kudva YC; Rajagopalan G; Raju R; Abraham RS; Smart M; Hanson J; David CS. 2002. Modulation of insulitis and type 1 diabetes by transgenic HLA-DR3 and DQ8 in NOD mice lacking endogenous MHC class II. Hum Immunol 63(11):987-99. [PubMed: 12392851]  [MGI Ref ID J:129453]

Ladel CH; Daugelat S; Kaufmann SH. 1995. Immune response to Mycobacterium bovis bacille Calmette Guerin infection in major histocompatibility complex class I- and II-deficient knock-out mice: contribution of CD4 and CD8 T cells to acquired resistance. Eur J Immunol 25(2):377-84. [PubMed: 7875199]  [MGI Ref ID J:112984]

Ladel CH; Flesch IE; Arnoldi J; Kaufmann SH. 1994. Studies with MHC-deficient knock-out mice reveal impact of both MHC I- and MHC II-dependent T cell responses on Listeria monocytogenes infection [published erratum appears in J Immunol 1995 Apr 15;154(8):4223] J Immunol 153(7):3116-22. [PubMed: 7726898]  [MGI Ref ID J:20420]

Lawson VJ; Maurice D; Silk JD; Cerundolo V; Weston K. 2009. Aberrant selection and function of invariant NKT cells in the absence of AP-1 transcription factor Fra-2. J Immunol 183(4):2575-84. [PubMed: 19620306]  [MGI Ref ID J:151549]

Lee BO; Rangel-Moreno J; Moyron-Quiroz JE; Hartson L; Makris M; Sprague F; Lund FE; Randall TD. 2005. CD4 T cell-independent antibody response promotes resolution of primary influenza infection and helps to prevent reinfection. J Immunol 175(9):5827-38. [PubMed: 16237075]  [MGI Ref ID J:119355]

Luhder F; Katz J; Benoist C; Mathis D. 1998. Major histocompatibility complex class II molecules can protect from diabetes by positively selecting T cells with additional specificities. J Exp Med 187(3):379-87. [PubMed: 9449718]  [MGI Ref ID J:108722]

Mangalam A; Luckey D; Basal E; Behrens M; Rodriguez M; David C. 2008. HLA-DQ6 (DQB1*0601)-restricted T cells protect against experimental autoimmune encephalomyelitis in HLA-DR3.DQ6 double-transgenic mice by generating anti-inflammatory IFN-gamma. J Immunol 180(11):7747-56. [PubMed: 18490779]  [MGI Ref ID J:136376]

Martin B; Bourgeois C; Dautigny N; Lucas B. 2003. On the role of MHC class II molecules in the survival and lymphopenia-induced proliferation of peripheral CD4+ T cells. Proc Natl Acad Sci U S A 100(10):6021-6. [PubMed: 12719530]  [MGI Ref ID J:126900]

Marusic-Galesic S; Walden P. 1995. Increased number of CD4-CD8+ MHC class II-specific T cells in MHC class II-deficient mice. Immunology 85(3):442-6. [PubMed: 7558133]  [MGI Ref ID J:110784]

Maurice D; Hooper J; Lang G; Weston K. 2007. c-Myb regulates lineage choice in developing thymocytes via its target gene Gata3. EMBO J 26(15):3629-40. [PubMed: 17641686]  [MGI Ref ID J:124106]

Millet V; Naquet P; Guinamard RR. 2008. Intercellular MHC transfer between thymic epithelial and dendritic cells. Eur J Immunol 38(5):1257-63. [PubMed: 18412162]  [MGI Ref ID J:134149]

Muller KP; Kyewski BA. 1995. Intrathymic T cell receptor (TcR) targeting in mice lacking CD4 or major histocompatibility complex (MHC) class II: rescue of CD4 T cell lineage without co-engagement of TcR/CD4 by MHC class II. Eur J Immunol 25(4):896-902. [PubMed: 7737291]  [MGI Ref ID J:24487]

Murray PD; McGavern DB; Lin X; Njenga MK; Leibowitz J; Pease LR; Rodriguez M. 1998. Perforin-dependent neurologic injury in a viral model of multiple sclerosis. J Neurosci 18(18):7306-14. [PubMed: 9736651]  [MGI Ref ID J:120427]

Nabozny GH; Baisch JM; Cheng S; Cosgrove D; Griffiths MM; Luthra HS; David CS. 1996. HLA-DQ8 transgenic mice are highly susceptible to collagen-induced arthritis: a novel model for human polyarthritis. J Exp Med 183(1):27-37. [PubMed: 8551230]  [MGI Ref ID J:88296]

Nasreen M; Ueno T; Saito F; Takahama Y. 2003. In vivo treatment of class II MHC-deficient mice with anti-TCR antibody restores the generation of circulating CD4 T cells and optimal architecture of thymic medulla. J Immunol 171(7):3394-400. [PubMed: 14500633]  [MGI Ref ID J:120396]

Natividad JM; Huang X; Slack E; Jury J; Sanz Y; David C; Denou E; Yang P; Murray J; McCoy KD; Verdu EF. 2009. Host responses to intestinal microbial antigens in gluten-sensitive mice. PLoS One 4(7):e6472. [PubMed: 19649259]  [MGI Ref ID J:151313]

Nooh MM; El-Gengehi N; Kansal R; David CS; Kotb M. 2007. HLA transgenic mice provide evidence for a direct and dominant role of HLA class II variation in modulating the severity of streptococcal sepsis. J Immunol 178(5):3076-83. [PubMed: 17312154]  [MGI Ref ID J:144098]

Pacholczyk R; Kraj P; Ignatowicz L. 2002. Peptide specificity of thymic selection of CD4+CD25+ T cells. J Immunol 168(2):613-20. [PubMed: 11777953]  [MGI Ref ID J:126012]

Pajot A; Michel ML; Fazilleau N; Pancre V; Auriault C; Ojcius DM; Lemonnier FA; Lone YC. 2004. A mouse model of human adaptive immune functions: HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice. Eur J Immunol 34(11):3060-9. [PubMed: 15468058]  [MGI Ref ID J:93846]

Pajot A; Pancre V; Fazilleau N; Michel ML; Angyalosi G; Ojcius DM; Auriault C; Lemonnier FA; Lone YC. 2004. Comparison of HLA-DR1-restricted T cell response induced in HLA-DR1 transgenic mice deficient for murine MHC class II and HLA-DR1 transgenic mice expressing endogenous murine MHC class II molecules. Int Immunol 16(9):1275-82. [PubMed: 15249541]  [MGI Ref ID J:132849]

Papouchado BG; Chapoval SP; Marietta EV; Weiler CR; David CS. 2001. Cockroach allergen-induced eosinophilic airway inflammation in HLA-DQ/human CD4(+) transgenic mice. J Immunol 167(8):4627-34. [PubMed: 11591792]  [MGI Ref ID J:130539]

Park SH; Roark JH; Bendelac A. 1998. Tissue-specific recognition of mouse CD1 molecules. J Immunol 160(7):3128-34. [PubMed: 9531267]  [MGI Ref ID J:46614]

Rajagopalan G; Iijima K; Singh M; Kita H; Patel R; David CS. 2006. Intranasal exposure to bacterial superantigens induces airway inflammation in HLA class II transgenic mice. Infect Immun 74(2):1284-96. [PubMed: 16428778]  [MGI Ref ID J:105070]

Rajagopalan G; Mangalam AK; Sen MM; Cheng S; Kudva YC; David CS. 2007. Autoimmunity in HLA-DQ8 transgenic mice expressing granulocyte/macrophage-colony stimulating factor in the beta cells of islets of Langerhans. Autoimmunity 40(3):169-79. [PubMed: 17453715]  [MGI Ref ID J:136978]

Rajagopalan G; Polich G; Sen MM; Singh M; Epstein BE; Lytle AK; Rouse MS; Patel R; David CS. 2008. Evaluating the role of HLA-DQ polymorphisms on immune response to bacterial superantigens using transgenic mice. Tissue Antigens 71(2):135-45. [PubMed: 18086265]  [MGI Ref ID J:148052]

Raju R; Munn SR; Majoribanks C; David CS. 1998. Islet cell autoimmunity in NOD mice transgenic to HLA-DQ8 and lacking I-Ag7. Transplant Proc 30(2):474. [PubMed: 9532134]  [MGI Ref ID J:110185]

Raju R; Spack EG; David CS. 2001. Acetylcholine receptor peptide recognition in HLA DR3-transgenic mice: in vivo responses correlate with MHC-peptide binding. J Immunol 167(2):1118-24. [PubMed: 11441124]  [MGI Ref ID J:132786]

Rincon M; Flavell RA. 1996. Regulation of AP-1 and NFAT transcription factors during thymic selection of T cells. Mol Cell Biol 16(3):1074-84. [PubMed: 8622652]  [MGI Ref ID J:106463]

Roddis M; Carter RW; Sun MY; Weissensteiner T; McMichael AJ; Bowness P; Bodmer HC. 2004. Fully functional HLA B27-restricted CD4+ as well as CD8+ T cell responses in TCR transgenic mice. J Immunol 172(1):155-61. [PubMed: 14688321]  [MGI Ref ID J:87093]

Saint-Mezard P; Chavagnac C; Vocanson M; Kehren J; Rozieres A; Bosset S; Ionescu M; Dubois B; Kaiserlian D; Nicolas JF; Berard F. 2005. Deficient Contact Hypersensitivity Reaction in CD4-/- Mice Is Because of Impaired Hapten-Specific CD8+ T Cell Functions. J Invest Dermatol 124(3):562-9. [PubMed: 15737197]  [MGI Ref ID J:96362]

Schaffer M; Bongartz M; Hoffmann W; Viebahn R. 2007. MHC-class-II-deficiency impairs wound healing. J Surg Res 138(1):100-5. [PubMed: 17161429]  [MGI Ref ID J:129590]

Schirmbeck R; Riedl P; Zurbriggen R; Akira S; Reimann J. 2003. Antigenic epitopes fused to cationic peptide bound to oligonucleotides facilitate Toll-like receptor 9-dependent, but CD4+ T cell help-independent, priming of CD8+ T cells. J Immunol 171(10):5198-207. [PubMed: 14607920]  [MGI Ref ID J:118774]

Schwandt T; Schumak B; Gielen GH; Jungerkes F; Schmidbauer P; Klocke K; Staratschek-Jox A; van Rooijen N; Kraal G; Ludwig-Portugall I; Franken L; Wehner S; Kalff JC; Weber O; Kirschning C; Coch C; Kalinke U; Wenzel J; Kurts C; Zawatzky R; Holzmann B; Layland L; Schultze JL; Burgdorf S; den Haan JM; Knolle PA; Limmer A. 2012. Expression of type I interferon by splenic macrophages suppresses adaptive immunity during sepsis. EMBO J 31(1):201-13. [PubMed: 22036947]  [MGI Ref ID J:180238]

Scotto M; Afonso G; Larger E; Raverdy C; Lemonnier FA; Carel JC; Dubois-Laforgue D; Baz B; Levy D; Gautier JF; Launay O; Bruno G; Boitard C; Sechi LA; Hutton JC; Davidson HW; Mallone R. 2012. Zinc transporter (ZnT)8(186-194) is an immunodominant CD8+ T cell epitope in HLA-A2+ type 1 diabetic patients. Diabetologia 55(7):2026-31. [PubMed: 22526607]  [MGI Ref ID J:188655]

Segura E; Nicco C; Lombard B; Veron P; Raposo G; Batteux F; Amigorena S; Thery C. 2005. ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming. Blood 106(1):216-23. [PubMed: 15790784]  [MGI Ref ID J:107454]

Sellge G; Magalhaes JG; Konradt C; Fritz JH; Salgado-Pabon W; Eberl G; Bandeira A; Di Santo JP; Sansonetti PJ; Phalipon A. 2010. Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity. J Immunol 184(4):2076-85. [PubMed: 20089698]  [MGI Ref ID J:159474]

Serbina NV; Lazarevic V; Flynn JL. 2001. CD4(+) T cells are required for the development of cytotoxic CD8(+) T cells during Mycobacterium tuberculosis infection. J Immunol 167(12):6991-7000. [PubMed: 11739519]  [MGI Ref ID J:73094]

Shen X; Niu C; Konig R. 2013. Increased numbers and suppressive activity of regulatory CD25(+)CD4(+) T lymphocytes in the absence of CD4 engagement by MHC class II molecules. Cell Immunol 282(2):117-28. [PubMed: 23770721]  [MGI Ref ID J:202111]

Sokol CL; Chu NQ; Yu S; Nish SA; Laufer TM; Medzhitov R. 2009. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. Nat Immunol 10(7):713-20. [PubMed: 19465907]  [MGI Ref ID J:150141]

Soong L; Chang CH; Sun J; Longley BJ Jr; Ruddle NH; Flavell RA; McMahon-Pratt D. 1997. Role of CD4+ T cells in pathogenesis associated with Leishmania amazonensis infection. J Immunol 158(11):5374-83. [PubMed: 9164958]  [MGI Ref ID J:64283]

Stohl W; Xu D; Kim KS; David CS; Allison JP. 2004. MHC class II-independent and -dependent T cell expansion and B cell hyperactivity in vivo in mice deficient in CD152 (CTLA-4). Int Immunol 16(7):895-904. [PubMed: 15136557]  [MGI Ref ID J:90671]

Stohl W; Xu D; Kim KS; Tedder TF; Sato S. 2005. Humoral autoimmunity in mice overexpressing B cell surface CD19: vital role for MHC class II. Clin Immunol 116(3):257-64. [PubMed: 15963762]  [MGI Ref ID J:100655]

Stohl W; Xu D; Metzger TE; Kim KS; Morel L; Kotzin BL. 2004. Dichotomous effects of complete versus partial class II major histocompatibility complex deficiency on circulating autoantibody levels in autoimmune-prone mice. Arthritis Rheum 50(7):2227-39. [PubMed: 15248222]  [MGI Ref ID J:106283]

Suzuki M; Takahashi T; Katano I; Ito R; Ito M; Harigae H; Ishii N; Sugamura K. 2012. Induction of human humoral immune responses in a novel HLA-DR-expressing transgenic NOD/Shi-scid/gammacnull mouse. Int Immunol 24(4):243-52. [PubMed: 22402880]  [MGI Ref ID J:182727]

Swier K; Brown DR; Bird JJ; Martin WD; Van Kaer L; Reiner SL. 1998. A critical, invariant chain-independent role for H2-M in antigen presentation. J Immunol 160(2):540-4. [PubMed: 9551884]  [MGI Ref ID J:45185]

Szanto S; Bardos T; Szabo Z; David CS; Buzas EI; Mikecz K; Glant TT. 2004. Induction of arthritis in HLA-DR4-humanized and HLA-DQ8-humanized mice by human cartilage proteoglycan aggrecan but only in the presence of an appropriate (non-MHC) genetic background. Arthritis Rheum 50(6):1984-95. [PubMed: 15188376]  [MGI Ref ID J:135879]

Taneja V; Behrens M; Cooper LT; Yamada S; Kita H; Redfield MM; Terzic A; David C. 2007. Spontaneous myocarditis mimicking human disease occurs in the presence of an appropriate MHC and non-MHC background in transgenic mice. J Mol Cell Cardiol 42(6):1054-64. [PubMed: 17499268]  [MGI Ref ID J:123115]

Taneja V; Griffiths M; Behrens M; Luthra HS; David CS. 2003. Auricular chondritis in NOD.DQ8.Abetao (Ag7-/-) transgenic mice resembles human relapsing polychondritis. J Clin Invest 112(12):1843-50. [PubMed: 14679179]  [MGI Ref ID J:86951]

Taneja V; Taneja N; Behrens M; Griffiths MM; Luthra HS; David CS. 2005. Requirement for CD28 may not be absolute for collagen-induced arthritis: study with HLA-DQ8 transgenic mice. J Immunol 174(2):1118-25. [PubMed: 15634938]  [MGI Ref ID J:95828]

Tilloy F; Treiner E; Park SH; Garcia C; Lemonnier F; de la Salle H; Bendelac A; Bonneville M; Lantz O. 1999. An invariant T cell receptor alpha chain defines a novel TAP-independent major histocompatibility complex class Ib-restricted alpha/beta T cell subpopulation in mammals. J Exp Med 189(12):1907-21. [PubMed: 10377186]  [MGI Ref ID J:55844]

Tonti E; Fedeli M; Napolitano A; Iannacone M; von Andrian UH; Guidotti LG; Abrignani S; Casorati G; Dellabona P. 2012. Follicular helper NKT cells induce limited B cell responses and germinal center formation in the absence of CD4(+) T cell help. J Immunol 188(7):3217-22. [PubMed: 22379027]  [MGI Ref ID J:183101]

Tonti E; Galli G; Malzone C; Abrignani S; Casorati G; Dellabona P. 2009. NKT-cell help to B lymphocytes can occur independently of cognate interaction. Blood 113(2):370-6. [PubMed: 18832653]  [MGI Ref ID J:144427]

Tonti E; Jimenez de Oya N; Galliverti G; Moseman EA; Di Lucia P; Amabile A; Sammicheli S; De Giovanni M; Sironi L; Chevrier N; Sitia G; Gennari L; Guidotti LG; von Andrian UH; Iannacone M. 2013. Bisphosphonates target B cells to enhance humoral immune responses. Cell Rep 5(2):323-30. [PubMed: 24120862]  [MGI Ref ID J:203773]

Tourne S; Miyazaki T; Wolf P; Ploegh H; Benoist C; Mathis D. 1997. Functionality of major histocompatibility complex class II molecules in mice doubly deficient for invariant chain and H-2M complexes. Proc Natl Acad Sci U S A 94(17):9255-60. [PubMed: 9256469]  [MGI Ref ID J:42482]

Treiner E; Duban L; Bahram S; Radosavljevic M; Wanner V; Tilloy F; Affaticati P; Gilfillan S; Lantz O. 2003. Selection of evolutionarily conserved mucosal-associated invariant T cells by MR1. Nature 422(6928):164-9. [PubMed: 12634786]  [MGI Ref ID J:113083]

Trobonjaca Z; Kroger A; Stober D; Leithauser F; Moller P; Hauser H; Schirmbeck R; Reimann J. 2002. Activating immunity in the liver. II. IFN-beta attenuates NK cell-dependent liver injury triggered by liver NKT cell activation. J Immunol 168(8):3763-70. [PubMed: 11937527]  [MGI Ref ID J:126190]

Ueno T; Saito F; Gray DH; Kuse S; Hieshima K; Nakano H; Kakiuchi T; Lipp M; Boyd RL; Takahama Y. 2004. CCR7 signals are essential for cortex-medulla migration of developing thymocytes. J Exp Med 200(4):493-505. [PubMed: 15302902]  [MGI Ref ID J:93960]

Vitry MA; De Trez C; Goriely S; Dumoutier L; Akira S; Ryffel B; Carlier Y; Letesson JJ; Muraille E. 2012. Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. Infect Immun 80(12):4271-80. [PubMed: 23006848]  [MGI Ref ID J:190620]

Vitry MA; Hanot Mambres D; De Trez C; Akira S; Ryffel B; Letesson JJ; Muraille E. 2014. Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis. J Immunol 192(8):3740-52. [PubMed: 24646742]  [MGI Ref ID J:209994]

Vocanson M; Hennino A; Cluzel-Tailhardat M; Saint-Mezard P; Benetiere J; Chavagnac C; Berard F; Kaiserlian D; Nicolas JF. 2006. CD8+ T cells are effector cells of contact dermatitis to common skin allergens in mice. J Invest Dermatol 126(4):815-20. [PubMed: 16456532]  [MGI Ref ID J:106764]

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de Candia P; Torri A; Gorletta T; Fedeli M; Bulgheroni E; Cheroni C; Marabita F; Crosti M; Moro M; Pariani E; Romano L; Esposito S; Mosca F; Rossetti G; Rossi RL; Geginat J; Casorati G; Dellabona P; Pagani M; Abrignani S. 2013. Intracellular modulation, extracellular disposal and serum increase of MiR-150 mark lymphocyte activation. PLoS One 8(9):e75348. [PubMed: 24205408]  [MGI Ref ID J:206477]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

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Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

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Breeding & Husbandry This strain is maintained homozygous x homozygous.

These mice are immunodeficient and need to be maintained in a high barrier environment with sterile food and water or given antibiotic (Sulfatrim) in the water.

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Cryopreserved

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Cryorecovery* $2140.00
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At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

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    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)