Description

Strain Information

Former Names 129S4/SvJaeSor-Pdgfrbtm11Sor/J    (Changed: 25-JUN-10 ) 129S4-Pdgfrbtm11Sor/J    (Changed: 25-JUN-10 ) Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Heterozygote         (Female x Male)   06-OCT-10 Species laboratory mouse Generation F?+N1F2 (30-JUN-11) Generation Definitions   Donating Investigator Dr. Philippe Soriano,   Mount Sinai School of Medicine

Description
These mice possess loxP sites on either side of the exons encoding the first and second immunoglobulin domain of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exons encoding the first and second immunoglobulin domain deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in adrenal tissue (see Stock No. 006364 for example), this mutant mouse strain may be useful in studies of steroidogenesis.

Development
A FRT site flanked targeting vector containing a PGKneo cassette was utilized in the construction of this mutant. A loxP site was inserted 500 bp upstream of the exon that encodes the first immunoglobulin domain. A second loxP site was inserted 2,100 bp downstream from the exon encoding the second immunoglobulin domain. This construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to mice carrying the Gt(ROSA)26Sor^tm1(FLP1)Dym allele (on the 129S4/SvJaeSor genetic background) to remove the FRT-flanked selection cassette. Mice that retained the loxP site flanked exons were then bred to 129S4/SvJaeSor mice to remove the FLP1 recombinase allele. Upon arrival at The Jackson Laboratory the mice were bred to 129S4/SvJaeJ inbred mice (Stock No. 009104) at least once to establish the colony.

Control Information

	Control
	Wild-type from the colony
	009104 129S4/SvJaeJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pdgfrb

007846	129S-Pdgfrbtm1Sor/J
018434	STOCK Pdgfrbtm13(Pdgfrb)Sor/J
018435	STOCK Pdgfrbtm14(Pdgfrb)Sor/J

View Strains carrying other alleles of Pdgfrb     (3 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Basal Ganglia Calcification, Idiopathic, 4; IBGC4   (PDGFRB) Myeloproliferative Disorder, Chronic, with Eosinophilia   (PDGFRB)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Pdgfrbtm11Sor
Allele Name		targeted mutation 11, Philippe Soriano
Allele Type		Targeted (Floxed/Frt)
Mutation Made By		Dr. Philippe Soriano,   Mount Sinai School of Medicine
Strain of Origin		129S4/SvJaeSor
Gene Symbol and Name		Pdgfrb, platelet derived growth factor receptor, beta polypeptide
Chromosome		18
Gene Common Name(s)		AI528809; CD140B; IBGC4; JTK12; PDGFR; PDGFR-1; PDGFR1; Pdgfr; expressed sequence AI528809; platelet derived growth factor receptor;
Molecular Note		LoxP sites were inserted at a BstEII and a HpaI site about 500 base pairs upstream of and 2,100 base pairs downstream from the exons encoding the first and second immunoglobulin domain, respectively. Additionally, a FRT-flanked PGKneo cassette was inserted and then removed by crossing with Gt(ROSA)26Sortm1(FLP1)Dym mice. [MGI Ref ID J:142042]

Genotyping

Genotyping Information

Genotyping Protocols

Pdgfrb^tm11Sor, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Schmahl J; Rizzolo K; Soriano P. 2008. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev 22(23):3255-67. [PubMed: 19056881]  [MGI Ref ID J:142042]

Additional References

Pdgfrb^tm11Sor related

Chintalgattu V; Ai D; Langley RR; Zhang J; Bankson JA; Shih TL; Reddy AK; Coombes KR; Daher IN; Pati S; Patel SS; Pocius JS; Taffet GE; Buja LM; Entman ML; Khakoo AY. 2010. Cardiomyocyte PDGFR-beta signaling is an essential component of the mouse cardiac response to load-induced stress. J Clin Invest 120(2):472-84. [PubMed: 20071776]  [MGI Ref ID J:156680]

Gromley A; Churchman ML; Zindy F; Sherr CJ. 2009. Transient expression of the Arf tumor suppressor during male germ cell and eye development in Arf-Cre reporter mice. Proc Natl Acad Sci U S A 106(15):6285-90. [PubMed: 19339492]  [MGI Ref ID J:147752]

Smith CL; Baek ST; Sung CY; Tallquist MD. 2011. Epicardial-derived cell epithelial-to-mesenchymal transition and fate specification require PDGF receptor signaling. Circ Res 108(12):e15-26. [PubMed: 21512159]  [MGI Ref ID J:185693]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Heterozygote x Heterozygote         (Female x Male)   06-OCT-10
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	009104 129S4/SvJaeJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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