Strain Name:

C57BL/6-Tg(Foxp3-DTR/EGFP)23.2Spar/Mmjax

Availability:

Repository- Live     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about C57BL/6-Tg(Foxp3-DTR/EGFP)23.2Spar/Mmjax MMRRC Stock Number 032050.
These "depletion of regulatory T cell" (DEREG) BAC transgenic mice express a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein under control of the endogenous forkhead box P3 promoter/enhancer regions on the BAC transgene. DTR-eGFP expression is observed in fully functional Foxp3+CD4+ regulatory T cell populations allowing fluorescent detection or diphtheria toxin-induced ablation Foxp3+ T reg cells.

Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   19-SEP-13
Mating SystemHemizygote x Noncarrier         (Female x Male)   19-SEP-13
Specieslaboratory mouse
Generation?+pN1F1 (16-JUL-14)
Generation Definitions
 
Donating Investigator Tim Sparwasser,   Twincore Institut fr Infektionsimmunol

Description
Mice hemizygous for the "depletion of regulatory T cell" (DEREG) BAC transgene are viable and fertile, with expression of a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein under control of the endogenous Foxp3 (forkhead box P3) promoter/enhancer regions on the BAC transgene. The donating investigator reports that transcription/translation from the BAC Foxp3 locus is disabled. These DEREG mice have DTR-eGFP expression in fully functional Foxp3+CD4+ regulatory T cell populations with no observed influence on regulation of the endogenous Foxp3 locus. Specifically, EGFP expression (via FACS) is observed mainly in CD25+CD4+ T cells, and allows specific detection of Foxp3+ regulatory T cell populations. The donating investigator also reports that EGFP expression is measurable by direct fluorescence. Diphtheria toxin (DT) administration results in ablation of Foxp3+CD4+ T reg cells with no apparent affect on CD25+ effector T cells. DT-induced depletion of Foxp3+CD4+ T reg cells is associated with enhanced and prolonged delayed-type hypersensitivity (DTH) responses and neonatal development of scurfy-like symptoms. The donating investigator reports that while homozygous females breed fine, homozygous males seem infertile.

Development
The RP23-267C15 bacterial artificial chromosome (BAC) containing at least ten mouse genes was obtained. This BAC was modified by targeting a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein and an SV40 polyA element into the first exon of the Foxp3 (forkhead box P3) locus: none of the other loci on the BAC were mutated. This modified ~150 kb BAC transgene was microinjected into the pronuclei of fertilized C57BL/6NCrl oocytes. Transgenic mice were identified and bred to C57BL/6J wildtype mice to establish the high transgene expressing founder line 23.2. These "depletion of regulatory T cell" (DEREG) mice were subsequently maintained by breeding transgenic mice with wildtype C57BL/6J or wildtype siblings for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Foxp3-DTR/EGFP)23.2Spar allele
011010   C.B6-Tg(Foxp3-DTR/EGFP)23.2Spar/Mmjax
View Strains carrying   Tg(Foxp3-DTR/EGFP)23.2Spar     (1 strain)

View Strains carrying other alleles of Foxp3     (16 strains)

Strains carrying other alleles of GFP
006053   129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
006067   129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006041   129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
016925   129;B6-Grin3b/Tmem259tm1Zhang Tg(Prnp-C19ORF6,-GFP)6Zhang/J
016251   129S.Cg-Tg(Hoxb7-EGFP)33Cos/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
017458   B6(C)-Tg(UAS-EGFP,-SOD1*G37R)135Gsn/J
017460   B6(C)-Tg(UAS-EGFP,-SOD1*G37R)677Gsn/J
008242   B6(Cg)-Gt(ROSA)26Sortm4(Ikbkb)Rsky/J
021011   B6(D2)-Tg(CAG-Brainbow1.0)2Eggn/J
021012   B6(D2)-Tg(CAG-Brainbow1.0)3Eggn/J
021469   B6(D2)-Tg(CAG-GFP,-Uprt)985Cdoe/J
016958   B6.129(Cg)-Foxp3tm3(DTR/GFP)Ayr/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
018979   B6.129(Cg)-Tg(CAG-EGFP)D4Nagy/KnwJ
010635   B6.129(FVB)-Alcamtm1Jawe/J
006071   B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
008606   B6.129-Gt(ROSA)26Sortm1Joe/J
006080   B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006075   B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
011036   B6.129-Hoxa11tm1Dmwe/J
008451   B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
005582   B6.129P-Cx3cr1tm1Litt/J
008710   B6.129P2(129S4)-Hprttm10(Ple162-EGFP/cre)Ems/Mmjax
008877   B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax
009114   B6.129P2(129S4)-Hprttm14(Ple103-EGFP/cre)Ems/Mmjax
008709   B6.129P2(129S4)-Hprttm9(Ple178-EGFP/cre)Ems/Mmjax
009113   B6.129P2(Cg)-Hprttm13(Ple54-EGFP)Ems/Mmjax
009115   B6.129P2(Cg)-Hprttm15(Ple111-EGFP)Ems/Mmjax
009118   B6.129P2(Cg)-Hprttm18(Ple90-EGFP)Ems/Mmjax
009353   B6.129P2(Cg)-Hprttm20(Ple53-EGFP)Ems/Mmjax
009596   B6.129P2(Cg)-Hprttm33(Ple183-EGFP)Ems/Mmjax
010770   B6.129P2(Cg)-Hprttm34(Ple186-EGFP)Ems/Mmjax
008706   B6.129P2(Cg)-Hprttm4(Ple88-EGFP)Ems/Mmjax
010789   B6.129P2(Cg)-Hprttm54(Ple233-EGFP)Ems/Mmjax
008707   B6.129P2(Cg)-Hprttm7(Ple185-EGFP)Ems/Mmjax
008708   B6.129P2(Cg)-Hprttm8(Ple151-EGFP)Ems/Mmjax
007766   B6.129P2(Cg)-Olfr160tm6Mom/MomJ
007572   B6.129P2(Cg)-Rorctm2Litt/J
005693   B6.129P2-Cxcr6tm1Litt/J
008875   B6.129P2-Lgr5tm1(cre/ERT2)Cle/J
016934   B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
025880   B6.129S-Tg(EEF1A1-SHC1*)1Ravi/J
021794   B6.129S1(Cg)-Ascl3tm1.1(EGFP/cre)Ovi/J
009380   B6.129S1-Irf4tm1Rdf/J
021930   B6.129S1-Tg(CAG-EGFP)S1C2Tpo/KnwPeaJ
022510   B6.129S4-Gpr88tm1.1(cre/GFP)Rpa/J
007669   B6.129S4-Pdgfratm11(EGFP)Sor/J
013061   B6.129S6-Ccr6tm1(EGFP)Irw/J
008379   B6.129S6-Il10tm1Flv/J
012644   B6.129S7-Pcdhgtm2Xzw/J
008466   B6.129X1(Cg)-Shhtm6Amc/J
009081   B6.129X1-Id1tm1Xhsu/J
006772   B6.Cg-Foxp3tm2Tch/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007906   B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
005491   B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
022486   B6.Cg-Ptprca Tg(UBC-PA-GFP)1Mnz/J
013115   B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
008705   B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
022148   B6.Cg-Tg(CSNK1D*,-EGFP)827Yfu/J
022149   B6.Cg-Tg(CSNK1D,-EGFP)432Yfu/J
023404   B6.Cg-Tg(CSNK1D,-EGFP)433Yfu/J
022787   B6.Cg-Tg(Chst4-EGFP)23Nrud/J
013134   B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J
017466   B6.Cg-Tg(Col1a1*3.6-Topaz)2Rowe/J
018306   B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
014135   B6.Cg-Tg(Fos/EGFP)1-3Brth/J
007673   B6.Cg-Tg(Gad1-EGFP)3Gfng/J
010835   B6.Cg-Tg(Gfap-EGFP)3739Sart/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
024808   B6.Cg-Tg(Inpp5d-EGFP)DLrr/CprJ
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
012643   B6.Cg-Tg(Ly6a-EGFP)G5Dzk/J
008323   B6.Cg-Tg(Mc4r-MAPT/Sapphire)21Rck/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
008321   B6.Cg-Tg(Npy-MAPT/Sapphire)1Rck/J
021232   B6.Cg-Tg(Nrl-EGFP)1Asw/J
008324   B6.Cg-Tg(Pmch-MAPT/CFP)1Rck/J
008322   B6.Cg-Tg(Pomc-MAPT/Topaz)1Rck/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
022086   B6.Cg-Tg(RP24-131B16/EGFP)13Ghan/J
019494   B6.Cg-Tg(RP24-131B16/EGFP)37Ghan/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
021614   B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J
012893   B6.Cg-Tg(S100a4-EGFP)M1Egn/YunkJ
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
016998   B6.Cg-Tg(TetO-Axin1,EGFP)TA6Cos/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
007919   B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ
021069   B6.Cg-Tg(Thy1-PA-GFP)5Rmpl/J
021070   B6.Cg-Tg(Thy1-PA-GFP)6Rmpl/J
015805   B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J
015806   B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J
015807   B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J
024688   B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J
025854   B6.FVB-Ptprca Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
008226   B6.FVB-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
018056   B6.FVB-Tg(CAG-boNT/B,-EGFP)U75-56Fwp/J
018055   B6.FVB-Tg(H2-K-S100a9,GFP)1Gabr/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
024033   B6.FVB-Tg(Shank3-EGFP)1Hzo/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
008126   B6.NOD-Tg(Cd4-EGFP)1Lt/J
023161   B6129S-Tg(Foxp3-EGFP/cre)1aJbs/J
024179   B6;129-Gt(ROSA)26Sortm1(Actb-T,-GFP)Dalco/J
008516   B6;129-Gt(ROSA)26Sortm1Joe/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
009600   B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J
008678   B6;129-Ubbtm1Rrk/J
010988   B6;129P-Cyp11a1tm1(GFP/cre)Pzg/J
010985   B6;129P-Klf3tm1(cre/ERT2)Pzg/J
015854   B6;129P2-Foxl2tm1(GFP/cre/ERT2)Pzg/J
008769   B6;129P2-Gpr15tm1.1Litt/J
012601   B6;129P2-Lyve1tm1.1(EGFP/cre)Cys/J
021162   B6;129P2-Mapttm2Arbr/J
006717   B6;129P2-Olfr124tm1Mom/MomJ
006665   B6;129P2-Olfr151tm13(rI7)Mom/MomJ
006666   B6;129P2-Olfr151tm24(Olfr2)Mom/MomJ
006676   B6;129P2-Olfr151tm26Mom/MomJ
006734   B6;129P2-Olfr151tm35(Adrb2)Mom/MomJ
006714   B6;129P2-Olfr160tm11(Olfr545)Mom/MomJ
006649   B6;129P2-Olfr17tm5(Olfr6)Mom/MomJ
006712   B6;129P2-Olfr545tm1Mom/MomJ
006715   B6;129P2-Olfr545tm3(Olfr160)Mom/MomJ
004946   B6;129P2-Omptm2(spH)Mom/J
006667   B6;129P2-Omptm3Mom/MomJ
006728   B6;129P2-Vmn2r26tm2Mom/MomJ
012735   B6;129S-Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze/J
010987   B6;129S-Sox18tm1(GFP/cre/ERT2)Pzg/J
017592   B6;129S-Sox2tm2Hoch/J
004858   B6;129S1-Tshrtm1Rmar/J
007843   B6;129S4-Efnb2tm2Sor/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
012464   B6;129S4-Foxd1tm2(GFP/cre/ERT2)Amc/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
008214   B6;129S4-Pou5f1tm2Jae/J
008078   B6;129S4-Tcf3tm5Zhu/J
017495   B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J
012436   B6;129S7-Tg(CAG-lacZ,-BMPR1A*,-EGFP)1Mis/Mmjax
008605   B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008080   B6;C3-Tg(CAG-SAC/EGFP)35Rang/J
010827   B6;C3-Tg(FOXJ1-EGFP)85Leo/J
010930   B6;CB-Tg(Pbsn-Hpn,-GFP)DVv/J
010704   B6;CBA-Tg(ATP6V1B1-EGFP)1Rnel/Mmjax
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
021588   B6;CBA-Tg(Gast-EGFP)1Tcw/J
007986   B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987   B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
007979   B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007980   B6;CBA-Tg(H/Olfr16-GFP)4Mom/MomJ
007981   B6;CBA-Tg(H/Olfr16-GFP)6Mom/MomJ
007984   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007982   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007983   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)9Mom/MomJ
007978   B6;CBA-Tg(Hf/Olfr16-GFP)47Mom/MomJ
007977   B6;CBA-Tg(Hf/Olfr16-GFP)7Mom/MomJ
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
011070   B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ
014651   B6;CBA-Tg(Thy1-spH)21Vnmu/J
015814   B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ
017494   B6;D-Tg(Tshz3-GFP/cre)43Amc/J
025122   B6;D2-Tg(RP24-330G11-EGFP)1Mik/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
014160   B6;DBA-Tg(S100b-EGFP/cre/ERT2)22Amc/J
014159   B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J
015855   B6;DBA-Tg(Upk3a-GFP/cre/ERT2)26Amc/J
009159   B6;FVB-Tg(Cnp-EGFP/Rpl10a)JD368Htz/J
021187   B6;FVB-Tg(Pbsn-rtTA*M2)42Xy/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
019381   B6;FVB-Tg(Zfp423-EGFP)7Brsp/J
021022   B6;SJL-Tg(AMELX-EGFP/RHOA*T19N)13Gibs/Mmjax
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
021078   B6N.129S1-Mrgprb4tm4.1(flpo)And/J
019013   B6N.129S6(Cg)-Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
018974   B6N.B6-Tg(Nr4a1-EGFP/cre)820Khog/J
018549   B6N.Cg-Tg(Csf1r-EGFP)1Hume/J
020650   B6N.Cg-Tg(Trpm8-EGFP)1Dmck/J
018913   B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J
007732   B6SJL-Tg(Dazl-hrGFP)4Gar/J
004190   C.129-Il4tm1Lky/J
005700   C.129P2-Cxcr6tm1Litt/J
006769   C.Cg-Foxp3tm2Tch/J
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008591   C57BL/6-Ackr3tm1Litt/J
023520   C57BL/6-Bcrtm1(BCR/ABL)Tsr/J
012343   C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J
012361   C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
010724   C57BL/6-Trim21tm1Hm/J
017469   C57BL/6-Tg(BGLAP-Topaz)1Rowe/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
017467   C57BL/6-Tg(Dmp1-Topaz)1Ikal/J
023800   C57BL/6-Tg(Foxp3-GFP)90Pkraj/J
012943   C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
016617   C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J
012890   C57BL/6-Tg(Scgb1a1-Il17f,GFP)1Cdon/J
007265   C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ
007264   C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
022476   C57BL/6-Tg(Uchl1-EGFP)G1Phoz/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
018895   C57BL/6J-Tg(Krt6,-cre,-Cerulean)1Grsr/Grsr
018896   C57BL/6J-Tg(Krt6,-cre,-Cerulean)2Grsr/Grsr
018898   C57BL/6J-Tg(Krt6,-cre,-Cerulean)4Grsr/Grsr
018899   C57BL/6J-Tg(Krt6,-cre,-Cerulean)5Grsr/Grsr
009593   C57BL/6J-Tg(Pomc-EGFP)1Low/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
019363   C57BL/6J-Tg(Trp63,-cre,-Cerulean)10Grsr/Grsr
018792   C57BL/6J-Tg(Trp63,-cre,-Cerulean)4Grsr/GrsrJ
024898   C57BL/6J-Tg(tetO-EGFP/Rpl10a)5aReij/J
018151   C57BL/6N-Krt17tm1(cre,Cerulean)Murr/GrsrJ
024753   C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J
024752   C57BL/6N-Tg(Ddx25-EGFP)1Mld/J
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003718   FVB-Tg(GadGFP)45704Swn/J
010947   FVB-Tg(Gstm5-EGFP)1Ilis/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
017484   FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
024636   FVB.B6-Tg(CAG-cat,-EGFP)1Rbns/KrnzJ
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
022735   FVB.Cg-Tg(Cspg4-EGFP*)HDbe/J
016573   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd Tg(S100B-EGFP)1Wjt Tg(SMN2*delta7)4299Ahmb/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
018393   FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J
009354   FVB/N-Tg(Dazl-EGFP)10Rarp/J
025062   FVB/N-Tg(Figla-EGFP,-icre)ZP3Dean/Mmjax
018548   FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
025097   NOD.129X1(Cg)-Foxp3tm2Tch/DvsJ
013116   NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
013233   NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
021937   NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CAG-EGFP)1Osb/SzJ
017619   NOD.Cg-Prkdcscid Tg(CAG-EGFP)1Osb/KupwJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
012881   STOCK Ascl1tm1Reed/J
008666   STOCK Fmn1tm1Made/J
016961   STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
025671   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(tetO-Fgf10)1Jaw/SpdlJ
024746   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Hprttm1(tetO-Dkk1)Spdl Tg(TCF/Lef1-lacZ)34Efu/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
009349   STOCK Hprttm31(Ple67-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
017530   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J
013749   STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
013751   STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
017701   STOCK Kiss1tm1.1(cre/EGFP)Stei/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
005692   STOCK Nphs1tm1Rkl/J
006702   STOCK Ntstm1Mom/MomJ
006622   STOCK Olfr151tm10Mom/MomJ
006646   STOCK Olfr151tm11(Olfr160)Mom/MomJ
006692   STOCK Olfr151tm16(Olfr160/Olfr161)Mom/MomJ
006627   STOCK Olfr151tm4Mom/MomJ
006626   STOCK Olfr151tm6Mom/MomJ
006625   STOCK Olfr151tm7Mom/MomJ
006624   STOCK Olfr151tm8Mom/MomJ
006623   STOCK Olfr151tm9Mom/MomJ
006740   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)AMom/MomJ
006741   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)BMom/MomJ
006647   STOCK Olfr160tm1(Olfr151)Mom/MomJ
006636   STOCK Olfr160tm5(Cnga2)Mom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006650   STOCK Olfr17tm6(Olfr713)Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
022757   STOCK Prg4tm1(GFP/cre/ERT2)Abl/J
006770   STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
006633   STOCK Vmn1r49tm3Mom/MomJ
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
017472   STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
017919   STOCK Tg(CAG-EGFP,-dsRed2/RNAi:Tardbp)6Zxu/J
011106   STOCK Tg(CAG-GFP*)1Hadj/J
013753   STOCK Tg(CAG-KikGR)33Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
019082   STOCK Tg(CMV-GFP,-BBS4)4T25Vcs/J
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
017468   STOCK Tg(Col1a1*3.6-Cyan)2Rowe/J
018322   STOCK Tg(Cp-EGFP)25Gaia/ReyaJ
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
017952   STOCK Tg(Isl1-EGFP*)1Slp/J
023965   STOCK Tg(Krt17-EGFP)#Cou/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
008579   STOCK Tg(PSCA-EGFP)1Witt/J
024578   STOCK Tg(Pax6-GFP/cre)1Rilm/J
023345   STOCK Tg(Pgk1-Ccnb1/EGFP)1Aklo/J
012276   STOCK Tg(Piwil2/EGFP)1Ghan/J
012277   STOCK Tg(Piwil4/EGFP)1Ghan/J
012452   STOCK Tg(Rr5-GFP/cre)1Sapc/J
006570   STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
018148   STOCK Tg(Slc17a8-EGFP)1Edw/SealJ
013752   STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J
003658   STOCK Tg(TIE2GFP)287Sato/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
025193   STOCK Tg(Vmn1r232-Mapt/EGFP)1Dlc/J
018281   STOCK Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax
006129   STOCK Tg(Zp3-EGFP)1Dean/J
023724   STOCK Tg(mI56i-cre,EGFP)1Kc/J
017755   STOCK Tg(tetO-GCAMP2)12iRyu/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
017918   STOCK Tg(tetO-MAML1*/EGFP)2Akar/J
017906   STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
View Strains carrying other alleles of GFP     (361 strains)

View Strains carrying other alleles of DTR     (6 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Foxp3-DTR/EGFP)23.2Spar/0

        involves: C57BL/6NCrl
  • immune system phenotype
  • *normal* immune system phenotype
    • mice exhibit normal numbers and frequencies of CD25+CD4+ T regulatory cells   (MGI Ref ID J:125295)
    • decreased regulatory T cell number
      • following diphtheria toxin treatment, CD4+ T regulatory cells are decreased in adult and neonates compared to in similarly treated wild-type mice   (MGI Ref ID J:125295)
      • however, T regulatory cell numbers rebound after cessation of diphtheria treatment in adult mice but not neonates   (MGI Ref ID J:125295)
    • enlarged lymph nodes
      • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
      • lymph node hyperplasia
        • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
    • enlarged spleen
      • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
    • increased inflammatory response
      • diphtheria toxin treated-neonates exhibit massive inflammatory infiltrate in various organs that resembles the phenotype of Foxp3sf hemizygotes   (MGI Ref ID J:125295)
      • increased susceptibility to type IV hypersensitivity reaction
        • following diphtheria toxin treatment, adult mice exhibit an enhanced and prolonged delayed-type hypersensitivity response compared with similarly treated wild-type mice   (MGI Ref ID J:125295)
      • insulitis
        • following diphtheria toxin treatment of neonates, mice exhibit insulitis and portal aggregates unlike similarly treated wild-type mice   (MGI Ref ID J:125295)
      • lung inflammation
        • following diphtheria toxin treatment of neonates, mice exhibit peribronchial and perivascular infiltrated unlike similarly treated wild-type mice   (MGI Ref ID J:125295)
      • skin inflammation
        • following diphtheria toxin treatment of neonates, the skin overlying the hyaline cartilage of the ear is thickened with epidermal hyperplasia and a dense lymphocyte infiltrate unlike in similarly treated wild-type mice   (MGI Ref ID J:125295)
    • increased spleen red pulp amount
      • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
    • increased spleen white pulp amount
      • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
  • endocrine/exocrine gland phenotype
  • insulitis
    • following diphtheria toxin treatment of neonates, mice exhibit insulitis and portal aggregates unlike similarly treated wild-type mice   (MGI Ref ID J:125295)
  • respiratory system phenotype
  • abnormal pulmonary alveolus morphology
    • following diphtheria toxin treatment of neonates, alveoli are delicate and have thin walls compared to in similarly treated wild-type mice   (MGI Ref ID J:125295)
  • lung inflammation
    • following diphtheria toxin treatment of neonates, mice exhibit peribronchial and perivascular infiltrated unlike similarly treated wild-type mice   (MGI Ref ID J:125295)
  • hematopoietic system phenotype
  • decreased regulatory T cell number
    • following diphtheria toxin treatment, CD4+ T regulatory cells are decreased in adult and neonates compared to in similarly treated wild-type mice   (MGI Ref ID J:125295)
    • however, T regulatory cell numbers rebound after cessation of diphtheria treatment in adult mice but not neonates   (MGI Ref ID J:125295)
  • enlarged spleen
    • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
  • increased spleen red pulp amount
    • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
  • increased spleen white pulp amount
    • following diphtheria toxin treatment of neonates   (MGI Ref ID J:125295)
  • integument phenotype
  • epidermal hyperplasia
    • following diphtheria toxin treatment of neonates, the skin overlying the hyaline cartilage of the ear is thickened with epidermal hyperplasia and a dense lymphocyte infiltrate unlike in similarly treated wild-type mice   (MGI Ref ID J:125295)
  • skin inflammation
    • following diphtheria toxin treatment of neonates, the skin overlying the hyaline cartilage of the ear is thickened with epidermal hyperplasia and a dense lymphocyte infiltrate unlike in similarly treated wild-type mice   (MGI Ref ID J:125295)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin
Immunodeficiency
      T cell deficiency
      specific T cell deficiency
Inflammation
Intracellular Signaling Molecules

Internal/Organ Research
Lymphoid Tissue Defects
      T cell deficiency

Research Tools
Cancer Research
      T cell deficiency
      production of T cells and hybridoma
      specific T cell deficiency
Fluorescent Proteins
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: T cell specific surface marker
Immunology, Inflammation and Autoimmunity Research
      T cell deficiency
      T cell specific surface marker
      production of T cell lines and hybridomas
      specific T cell deficiency

GFP related

Research Tools
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Foxp3-DTR/EGFP)23.2Spar
Allele Name transgene insertion 23.2, Tim Sparwasser
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) DEREG; Tg(Foxp3-DTR/EGFP)1Ebe;
Mutation Made By Tim Sparwasser,   Twincore Inst. for Infection Immunology
Strain of OriginC57BL/6NCrl
Site of ExpressionSelective DTR-eGFP expression is seen within the CD4+ T cell compartment, and highest expression within the CD25+ subset.
Expressed Gene DTR, Simian Diphtheria Toxin Receptor,
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Promoter Foxp3, forkhead box P3, mouse, laboratory
Molecular Note The RP23-267C15 bacterial artificial chromosome (BAC) containing at least ten mouse genes was obtained. This BAC was modified by targeting a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein and an SV40 polyA element into the first exon of the Foxp3 (forkhead box P3) locus: none of the other loci on the BAC were mutated. Two high expressing lines (16.1 and 23.2) were established. Analysis revealed selective DTR-eGFP expressionwithin the CD4+ T cell compartment, with the highest expression within the CD25+ subset. [MGI Ref ID J:125295]
 
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Fluorescent Proteins -- Generic GFP, QPCR
Tg(Foxp3-DTR/EGFP)23.2Spar, High Resolution Melting
Tg(Foxp3-DTR/EGFP)23.2Spar, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jung S; Unutmaz D; Wong P; Sano G; De los Santos K; Sparwasser T; Wu S; Vuthoori S; Ko K; Zavala F; Pamer EG; Littman DR; Lang RA. 2002. In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens. Immunity 17(2):211-20. [PubMed: 12196292]  [MGI Ref ID J:93488]

Lahl K; Loddenkemper C; Drouin C; Freyer J; Arnason J; Eberl G; Hamann A; Wagner H; Huehn J; Sparwasser T. 2007. Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease. J Exp Med 204(1):57-63. [PubMed: 17200412]  [MGI Ref ID J:125295]

Additional References

Tg(Foxp3-DTR/EGFP)23.2Spar related

Abel S; Luckheide N; Westendorf AM; Geffers R; Roers A; Muller W; Sparwasser T; Matuschewski K; Buer J; Hansen W. 2012. Strong impact of CD4+ Foxp3+ regulatory T cells and limited effect of T cell-derived IL-10 on pathogen clearance during Plasmodium yoelii infection. J Immunol 188(11):5467-77. [PubMed: 22544931]  [MGI Ref ID J:188734]

Anz D; Koelzer VH; Moder S; Thaler R; Schwerd T; Lahl K; Sparwasser T; Besch R; Poeck H; Hornung V; Hartmann G; Rothenfusser S; Bourquin C; Endres S. 2010. Immunostimulatory RNA blocks suppression by regulatory T cells. J Immunol 184(2):939-46. [PubMed: 19966212]  [MGI Ref ID J:159428]

Baru AM; Ganesh V; Krishnaswamy JK; Hesse C; Untucht C; Glage S; Behrens G; Mayer CT; Puttur F; Sparwasser T. 2012. Absence of Foxp3+ regulatory T cells during allergen provocation does not exacerbate murine allergic airway inflammation. PLoS One 7(10):e47102. [PubMed: 23071726]  [MGI Ref ID J:192084]

Beyer M; Thabet Y; Muller RU; Sadlon T; Classen S; Lahl K; Basu S; Zhou X; Bailey-Bucktrout SL; Krebs W; Schonfeld EA; Bottcher J; Golovina T; Mayer CT; Hofmann A; Sommer D; Debey-Pascher S; Endl E; Limmer A; Hippen KL; Blazar BR; Balderas R; Quast T; Waha A; Mayer G; Famulok M; Knolle PA; Wickenhauser C; Kolanus W; Schermer B; Bluestone JA; Barry SC; Sparwasser T; Riley JL; Schultze JL. 2011. Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. Nat Immunol 12(9):898-907. [PubMed: 21841785]  [MGI Ref ID J:176464]

Brincks EL; Roberts AD; Cookenham T; Sell S; Kohlmeier JE; Blackman MA; Woodland DL. 2013. Antigen-Specific Memory Regulatory CD4+Foxp3+ T Cells Control Memory Responses to Influenza Virus Infection. J Immunol 190(7):3438-46. [PubMed: 23467933]  [MGI Ref ID J:194913]

Cassani B; Villablanca EJ; Quintana FJ; Love PE; Lacy-Hulbert A; Blaner WS; Sparwasser T; Snapper SB; Weiner HL; Mora JR. 2011. Gut-tropic T cells that express integrin alpha4beta7 and CCR9 are required for induction of oral immune tolerance in mice. Gastroenterology 141(6):2109-18. [PubMed: 21925467]  [MGI Ref ID J:180414]

Cervantes-Barragan L; Firner S; Bechmann I; Waisman A; Lahl K; Sparwasser T; Thiel V; Ludewig B. 2012. Regulatory T cells selectively preserve immune privilege of self-antigens during viral central nervous system infection. J Immunol 188(8):3678-85. [PubMed: 22407917]  [MGI Ref ID J:184079]

Dietze KK; Zelinskyy G; Gibbert K; Schimmer S; Francois S; Myers L; Sparwasser T; Hasenkrug KJ; Dittmer U. 2011. Transient depletion of regulatory T cells in transgenic mice reactivates virus-specific CD8+ T cells and reduces chronic retroviral set points. Proc Natl Acad Sci U S A 108(6):2420-5. [PubMed: 21262821]  [MGI Ref ID J:169103]

Ehrentraut H; Westrich JA; Eltzschig HK; Clambey ET. 2012. Adora2b adenosine receptor engagement enhances regulatory T cell abundance during endotoxin-induced pulmonary inflammation. PLoS One 7(2):e32416. [PubMed: 22389701]  [MGI Ref ID J:185292]

Fyhrquist N; Lehtimaki S; Lahl K; Savinko T; Lappetelainen AM; Sparwasser T; Wolff H; Lauerma A; Alenius H. 2012. Foxp3+ cells control Th2 responses in a murine model of atopic dermatitis. J Invest Dermatol 132(6):1672-80. [PubMed: 22402436]  [MGI Ref ID J:188756]

Goldwich A; Steinkasserer A; Gessner A; Amann K. 2012. Impairment of podocyte function by diphtheria toxin-a new reversible proteinuria model in mice. Lab Invest 92(12):1674-85. [PubMed: 23007132]  [MGI Ref ID J:190336]

Hall JA; Cannons JL; Grainger JR; Dos Santos LM; Hand TW; Naik S; Wohlfert EA; Chou DB; Oldenhove G; Robinson M; Grigg ME; Kastenmayer R; Schwartzberg PL; Belkaid Y. 2011. Essential Role for Retinoic Acid in the Promotion of CD4(+) T Cell Effector Responses via Retinoic Acid Receptor Alpha. Immunity 34(3):435-47. [PubMed: 21419664]  [MGI Ref ID J:169861]

Hansen W; Hutzler M; Abel S; Alter C; Stockmann C; Kliche S; Albert J; Sparwasser T; Sakaguchi S; Westendorf AM; Schadendorf D; Buer J; Helfrich I. 2012. Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J Exp Med 209(11):2001-16. [PubMed: 23045606]  [MGI Ref ID J:190913]

Huang YJ; Haist V; Baumgartner W; Fohse L; Prinz I; Suerbaum S; Floess S; Huehn J. 2014. Induced and thymus-derived Foxp3(+) regulatory T cells share a common niche. Eur J Immunol 44(2):460-8. [PubMed: 24170313]  [MGI Ref ID J:208910]

Hubert S; Rissiek B; Klages K; Huehn J; Sparwasser T; Haag F; Koch-Nolte F; Boyer O; Seman M; Adriouch S. 2010. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med 207(12):2561-8. [PubMed: 20975043]  [MGI Ref ID J:176876]

Kanjarawi R; Dy M; Bardel E; Sparwasser T; Dubois B; Mecheri S; Kaiserlian D. 2013. Regulatory CD4+Foxp3+ T cells control the severity of anaphylaxis. PLoS One 8(7):e69183. [PubMed: 23922690]  [MGI Ref ID J:203276]

Kim J; Lahl K; Hori S; Loddenkemper C; Chaudhry A; deRoos P; Rudensky A; Sparwasser T. 2009. Cutting edge: depletion of Foxp3+ cells leads to induction of autoimmunity by specific ablation of regulatory T cells in genetically targeted mice. J Immunol 183(12):7631-4. [PubMed: 19923467]  [MGI Ref ID J:157495]

Klages K; Mayer CT; Lahl K; Loddenkemper C; Teng MW; Ngiow SF; Smyth MJ; Hamann A; Huehn J; Sparwasser T. 2010. Selective depletion of Foxp3+ regulatory T cells improves effective therapeutic vaccination against established melanoma. Cancer Res 70(20):7788-99. [PubMed: 20924102]  [MGI Ref ID J:165566]

Kleinschnitz C; Kraft P; Dreykluft A; Hagedorn I; Gobel K; Schuhmann MK; Langhauser F; Helluy X; Schwarz T; Bittner S; Mayer CT; Brede M; Varallyay C; Pham M; Bendszus M; Jakob P; Magnus T; Meuth SG; Iwakura Y; Zernecke A; Sparwasser T; Nieswandt B; Stoll G; Wiendl H. 2013. Regulatory T cells are strong promoters of acute ischemic stroke in mice by inducing dysfunction of the cerebral microvasculature. Blood 121(4):679-91. [PubMed: 23160472]  [MGI Ref ID J:194093]

Klingenberg R; Gerdes N; Badeau RM; Gistera A; Strodthoff D; Ketelhuth DF; Lundberg AM; Rudling M; Nilsson SK; Olivecrona G; Zoller S; Lohmann C; Luscher TF; Jauhiainen M; Sparwasser T; Hansson GK. 2013. Depletion of FOXP3+ regulatory T cells promotes hypercholesterolemia and atherosclerosis. J Clin Invest 123(3):1323-34. [PubMed: 23426179]  [MGI Ref ID J:196393]

Kuhlhorn F; Rath M; Schmoeckel K; Cziupka K; Nguyen HH; Hildebrandt P; Hunig T; Sparwasser T; Huehn J; Potschke C; Broker BM. 2013. Foxp3+ regulatory T cells are required for recovery from severe sepsis. PLoS One 8(5):e65109. [PubMed: 23724126]  [MGI Ref ID J:200788]

Lahl K; Mayer CT; Bopp T; Huehn J; Loddenkemper C; Eberl G; Wirnsberger G; Dornmair K; Geffers R; Schmitt E; Buer J; Sparwasser T. 2009. Nonfunctional regulatory T cells and defective control of Th2 cytokine production in natural scurfy mutant mice. J Immunol 183(9):5662-72. [PubMed: 19812199]  [MGI Ref ID J:156808]

Lehtimaki S; Savinko T; Lahl K; Sparwasser T; Wolff H; Lauerma A; Alenius H; Fyhrquist N. 2012. The temporal and spatial dynamics of Foxp3+ Treg cell-mediated suppression during contact hypersensitivity responses in a murine model. J Invest Dermatol 132(12):2744-51. [PubMed: 22739792]  [MGI Ref ID J:191693]

Markey KA; Koyama M; Kuns RD; Lineburg KE; Wilson YA; Olver SD; Raffelt NC; Don AL; Varelias A; Robb RJ; Cheong M; Engwerda CR; Steptoe RJ; Ramshaw HS; Lopez AF; Vega-Ramos J; Lew AM; Villadangos JA; Hill GR; MacDonald KP. 2012. Immune insufficiency during GVHD is due to defective antigen presentation within dendritic cell subsets. Blood 119(24):5918-30. [PubMed: 22415754]  [MGI Ref ID J:186569]

Mattarollo SR; Yong M; Gosmann C; Choyce A; Chan D; Leggatt GR; Frazer IH. 2011. NKT cells inhibit antigen-specific effector CD8 T cell induction to skin viral proteins. J Immunol 187(4):1601-8. [PubMed: 21742969]  [MGI Ref ID J:179166]

Mayer CT; Kuhl AA; Loddenkemper C; Sparwasser T. 2012. Lack of Foxp3+ macrophages in both untreated and B16 melanoma-bearing mice. Blood 119(5):1314-5. [PubMed: 22308282]  [MGI Ref ID J:181693]

McNally A; Hill GR; Sparwasser T; Thomas R; Steptoe RJ. 2011. CD4+CD25+ regulatory T cells control CD8+ T-cell effector differentiation by modulating IL-2 homeostasis. Proc Natl Acad Sci U S A 108(18):7529-34. [PubMed: 21502514]  [MGI Ref ID J:172217]

Navid F; Boniotto M; Walker C; Ahrens K; Proksch E; Sparwasser T; Muller W; Schwarz T; Schwarz A. 2012. Induction of regulatory T cells by a murine beta-defensin. J Immunol 188(2):735-43. [PubMed: 22174455]  [MGI Ref ID J:180788]

Navid F; Bruhs A; Schuller W; Fritsche E; Krutmann J; Schwarz T; Schwarz A. 2013. The Aryl Hydrocarbon Receptor Is Involved in UVR-Induced Immunosuppression. J Invest Dermatol 133(12):2763-70. [PubMed: 23652795]  [MGI Ref ID J:203019]

Ohkura N; Hamaguchi M; Morikawa H; Sugimura K; Tanaka A; Ito Y; Osaki M; Tanaka Y; Yamashita R; Nakano N; Huehn J; Fehling HJ; Sparwasser T; Nakai K; Sakaguchi S. 2012. T cell receptor stimulation-induced epigenetic changes and Foxp3 expression are independent and complementary events required for Treg cell development. Immunity 37(5):785-99. [PubMed: 23123060]  [MGI Ref ID J:190981]

Oldenhove G; Bouladoux N; Wohlfert EA; Hall JA; Chou D; Dos Santos L; O'Brien S; Blank R; Lamb E; Natarajan S; Kastenmayer R; Hunter C; Grigg ME; Belkaid Y. 2009. Decrease of Foxp3+ Treg cell number and acquisition of effector cell phenotype during lethal infection. Immunity 31(5):772-86. [PubMed: 19896394]  [MGI Ref ID J:155313]

Osorio F; Leibundgut-Landmann S; Lochner M; Lahl K; Sparwasser T; Eberl G; Reis E Sousa C. 2008. DC activated via dectin-1 convert Treg into IL-17 producers. Eur J Immunol 38(12):3274-3281. [PubMed: 19039774]  [MGI Ref ID J:141368]

Paust HJ; Ostmann A; Erhardt A; Turner JE; Velden J; Mittrucker HW; Sparwasser T; Panzer U; Tiegs G. 2011. Regulatory T cells control the Th1 immune response in murine crescentic glomerulonephritis. Kidney Int 80(2):154-64. [PubMed: 21525855]  [MGI Ref ID J:194807]

Pellegrini M; Calzascia T; Elford AR; Shahinian A; Lin AE; Dissanayake D; Dhanji S; Nguyen LT; Gronski MA; Morre M; Assouline B; Lahl K; Sparwasser T; Ohashi PS; Mak TW. 2009. Adjuvant IL-7 antagonizes multiple cellular and molecular inhibitory networks to enhance immunotherapies. Nat Med 15(5):528-36. [PubMed: 19396174]  [MGI Ref ID J:149567]

Robb RJ; Lineburg KE; Kuns RD; Wilson YA; Raffelt NC; Olver SD; Varelias A; Alexander KA; Teal BE; Sparwasser T; Hammerling GJ; Markey KA; Koyama M; Clouston AD; Engwerda CR; Hill GR; MacDonald KP. 2012. Identification and expansion of highly suppressive CD8(+)FoxP3(+) regulatory T cells after experimental allogeneic bone marrow transplantation. Blood 119(24):5898-908. [PubMed: 22538855]  [MGI Ref ID J:188644]

Schallenberg S; Petzold C; Tsai PY; Sparwasser T; Kretschmer K. 2012. Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells. PLoS One 7(8):e41971. [PubMed: 22879902]  [MGI Ref ID J:189833]

Schuster M; Glauben R; Plaza-Sirvent C; Schreiber L; Annemann M; Floess S; Kuhl AA; Clayton LK; Sparwasser T; Schulze-Osthoff K; Pfeffer K; Huehn J; Siegmund B; Schmitz I. 2012. IkappaB(NS) Protein Mediates Regulatory T Cell Development via Induction of the Foxp3 Transcription Factor. Immunity 37(6):998-1008. [PubMed: 23200824]  [MGI Ref ID J:191062]

Schwarz A; Navid F; Sparwasser T; Clausen BE; Schwarz T. 2012. 1,25-dihydroxyvitamin D exerts similar immunosuppressive effects as UVR but is dispensable for local UVR-induced immunosuppression. J Invest Dermatol 132(12):2762-9. [PubMed: 22854622]  [MGI Ref ID J:191596]

Seung E; Cho JL; Sparwasser T; Medoff BD; Luster AD. 2011. Inhibiting CXCR3-dependent CD8+ T cell trafficking enhances tolerance induction in a mouse model of lung rejection. J Immunol 186(12):6830-8. [PubMed: 21555535]  [MGI Ref ID J:175494]

Stagg J; Divisekera U; Duret H; Sparwasser T; Teng MW; Darcy PK; Smyth MJ. 2011. CD73-Deficient Mice Have Increased Antitumor Immunity and Are Resistant to Experimental Metastasis. Cancer Res 71(8):2892-2900. [PubMed: 21292811]  [MGI Ref ID J:171004]

Steeg C; Adler G; Sparwasser T; Fleischer B; Jacobs T. 2009. Limited role of CD4+Foxp3+ regulatory T cells in the control of experimental cerebral malaria. J Immunol 183(11):7014-22. [PubMed: 19890049]  [MGI Ref ID J:157400]

Stein P; Weber M; Prufer S; Schmid B; Schmitt E; Probst HC; Waisman A; Langguth P; Schild H; Radsak MP. 2011. Regulatory T cells and IL-10 independently counterregulate cytotoxic T lymphocyte responses induced by transcutaneous immunization. PLoS One 6(11):e27911. [PubMed: 22114725]  [MGI Ref ID J:180954]

Teng MW; Ngiow SF; von Scheidt B; McLaughlin N; Sparwasser T; Smyth MJ. 2010. Conditional regulatory T-cell depletion releases adaptive immunity preventing carcinogenesis and suppressing established tumor growth. Cancer Res 70(20):7800-9. [PubMed: 20924111]  [MGI Ref ID J:165562]

Vaeth M; Gogishvili T; Bopp T; Klein M; Berberich-Siebelt F; Gattenloehner S; Avots A; Sparwasser T; Grebe N; Schmitt E; Hunig T; Serfling E; Bodor J. 2011. Regulatory T cells facilitate the nuclear accumulation of inducible cAMP early repressor (ICER) and suppress nuclear factor of activated T cell c1 (NFATc1). Proc Natl Acad Sci U S A 108(6):2480-5. [PubMed: 21262800]  [MGI Ref ID J:169110]

Veiga-Parga T; Suryawanshi A; Mulik S; Gimenez F; Sharma S; Sparwasser T; Rouse BT. 2012. On the Role of Regulatory T Cells during Viral-Induced Inflammatory Lesions. J Immunol 189(12):5924-33. [PubMed: 23129753]  [MGI Ref ID J:190970]

Villegas-Mendez A; de Souza JB; Lavelle SW; Gwyer Findlay E; Shaw TN; van Rooijen N; Saris CJ; Hunter CA; Riley EM; Couper KN. 2013. IL-27 receptor signalling restricts the formation of pathogenic, terminally differentiated Th1 cells during malaria infection by repressing IL-12 dependent signals. PLoS Pathog 9(4):e1003293. [PubMed: 23593003]  [MGI Ref ID J:196654]

Wang Y; Sparwasser T; Figlin R; Kim HL. 2014. Foxp3+ T cells inhibit antitumor immune memory modulated by mTOR inhibition. Cancer Res 74(8):2217-28. [PubMed: 24574514]  [MGI Ref ID J:210527]

Wheeler K; Tardif S; Rival C; Luu B; Bui E; Del Rio R; Teuscher C; Sparwasser T; Hardy D; Tung KS. 2011. Regulatory T cells control tolerogenic versus autoimmune response to sperm in vasectomy. Proc Natl Acad Sci U S A 108(18):7511-6. [PubMed: 21502500]  [MGI Ref ID J:172046]

Zelinskyy G; Dietze KK; Husecken YP; Schimmer S; Nair S; Werner T; Gibbert K; Kershaw O; Gruber AD; Sparwasser T; Dittmer U. 2009. The regulatory T-cell response during acute retroviral infection is locally defined and controls the magnitude and duration of the virus-specific cytotoxic T-cell response. Blood 114(15):3199-207. [PubMed: 19671923]  [MGI Ref ID J:153544]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator reports that while homozygous females breed fine, homozygous males seem infertile.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   19-SEP-13
Hemizygote x Noncarrier         (Female x Male)   19-SEP-13
Diet Information LabDiet® 5K20

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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