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| These ROSA:LNL:tTA mutant mice harbor a conditional mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of the downstream optimized tetracycline-controlled transactivator protein. Applying both Cre-lox and Tet-Off technologies, these mutant mice may be useful for generating compound mutant mice in which expression of a tetracycline-responsive promoter element-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Gene Trap; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 13-JUL-12 Species laboratory mouse Generation N4F5 (25-FEB-13)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).Development
The ROSA:LNL:tTA targeting vector was designed to insert a loxP-flanked STOP cassette (containing a neomycin resistance cassette and transcriptional STOP element) just upstream of a optimized form of the tetracycline controlled transactivator ("mtTA") into the Gt(ROSA)26Sor locus. The donating investigator reports that the construct was electroporated into 129P2/OlaHsd-derived HM-1 embryonic stem (ES) cells, correctly targeted ES cells were injected into recipient blastocysts and the resultant chimeric mice were bred to C57BL/6J mice to establish the colony. Mutant mice on a mixed genetic background (mostly C57BL/6 with some 129 or FVB/N) were sent to The Jackson Laboratory (as Stock No. 008600). Upon arrival, some mice were bred with C57BL/6J using a marker-assisted, speed congenic approach to generate this congenic strain (Stock No. 011008).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(tTA)Roos allele
008603 C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J 008600 STOCK Gt(ROSA)26Sortm1(tTA)Roos/J View Strains carrying Gt(ROSA)26Sortm1(tTA)Roos (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Tet Expression System
tTA/rtTA Expressing Strains
Research Tools
Cancer Research
Tetop Tet System
Cardiovascular Research
Cre-lox System
Tetop Tet System
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Cre-lox System
Tetop Tet System
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(tTA)Roos | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Raymond Roos | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | ROSA:LNL:tTA; | ||
| Mutation Made By | Raymond Roos, University of Chicago | ||
| Strain of Origin | 129P2/OlaHsd-Hprt | ||
| ES Cell Line Name | HM-1 | ||
| ES Cell Line Strain | 129P2/OlaHsd-Hprt | ||
| Site of Expression | Widespread expression of the tetracycline-controlled transactivator (tTA) protein is initiated after removal of an upstream loxP-flanked STOP cassette by Cre recombinase. Thus the expression pattern of tTA is defined by the expression pattern of Cre recombinase. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Molecular Note | A targeting vector was designed to insert a loxP-flanked STOP cassette (containing a neomycin resistance cassette and transcriptional STOP element) just upstream of a modified/optimized form of tetracycline controlled transactivator ("mtTA") into the Gt(ROSA)26Sor locus. and chimeric mice were established. [MGI Ref ID J:138761] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(tTA)Roos, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wang L; Sharma K; Deng HX; Siddique T; Grisotti G; Liu E; Roos RP. 2008. Restricted expression of mutant SOD1 in spinal motor neurons and interneurons induces motor neuron pathology. Neurobiol Dis 29(3):400-8. [PubMed: 18054242] [MGI Ref ID J:138761]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry Mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) using a marker-assisted, speed congenic approach to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 13-JUL-12 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(tTA)Roos
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Gt(ROSA)26Sortm1(tTA)Roos x Homozygous for Gt(ROSA)26Sortm1(tTA)Roos Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(tTA)Roos
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Gt(ROSA)26Sortm1(tTA)Roos x Homozygous for Gt(ROSA)26Sortm1(tTA)Roos Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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