Strain Name:

B6;129-Ndptm1.1Nat/J

Stock Number:

011076

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
The human placental alkaline phosphatase (AP) reporter was introduced to the Ndp (Norrie disease (pseudoglioma) (human)) locus. The coding region is intact but it is not apparently expressed. As a result, the retinal hypovascularization defect caused by this allele is as severe as that seen with a conventional null allele. This strain may be useful in studies of vascular growth, remodeling, maintenance, and disease.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Ndptm1Nat/J    (Changed: 09-APR-10 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating Investigator Jeremy Nathans,   Johns Hopkins University

Description
The human placental alkaline phosphatase (AP) reporter was introduced upstream of the Ndp (Norrie disease (pseudoglioma) (human)) locus start codon. The coding region is intact but it is not apparently expressed and as a result, the retinal hypovascularization defect caused by this allele is as severe as that seen with a conventional null allele. Retinas exhibit retarded vascular growth and sparse vascular coverage. Histochemical staining for AP in males shows homogenous staining throughout the retina at postnatal day 4 (P4), P7 and in adulthood. AP expression in females is mosaic as a result of X chromosome inactivation, and may be useful in visualizing individual cell morphologies. This strain may be useful in studies of vascular growth, remodeling, maintenance, and disease.

Development
The coding sequence of human placenta alkaline phosphatase gene and rabbit beta-globin 3' UTR followed by a PGK-neomycin resistance cassette flanked by FRT sites were inserted upstream of the start codon of the targeted gene (exon 2, 5' UTR). The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice were crossed to a germline FLP strain to excise the neomycin cassette. The strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ndp
012287   B6;129-Ndptm2Nat/J
View Strains carrying other alleles of Ndp     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Exudative Vitreoretinopathy 2, X-Linked; EVR2   (NDP)
Norrie Disease; ND   (NDP)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ndptm1.1Nat/Ndp+

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
  • no phenotypic analysis
  • *normal* no phenotypic analysis
    • mice were used for expression analysis   (MGI Ref ID J:154020)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Reproductive Biology Research
Fertility Defects
      females only

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ndptm1.1Nat
Allele Name targeted mutation 1.1, Jeremy Nathans
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) NdpAP;
Mutation Made By Jeremy Nathans,   Johns Hopkins University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Ndp, Norrie disease (pseudoglioma) (human)
Chromosome X
Gene Common Name(s) EVR2; FEVR; ND; Ndph; Norrie disease homolog; Norrin;
Molecular Note An alkaline phosphatase sequence was inserted in exon2 along with an frt flanked PGK-neomycin cassette. Excision of the PGK-neo cassette was accomplished by crossing mice to Tg(ACTFLPe)9205Dym. [MGI Ref ID J:154020]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ye X; Wang Y; Cahill H; Yu M; Badea TC; Smallwood PM; Peachey NS; Nathans J. 2009. Norrin, frizzled-4, and Lrp5 signaling in endothelial cells controls a genetic program for retinal vascularization. Cell 139(2):285-98. [PubMed: 19837032]  [MGI Ref ID J:154020]

Additional References

Ndptm1.1Nat related

Ye X; Smallwood P; Nathans J. 2011. Expression of the Norrie disease gene (Ndp) in developing and adult mouse eye, ear, and brain. Gene Expr Patterns 11(1-2):151-5. [PubMed: 21055480]  [MGI Ref ID J:168626]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, hemizygous males and heterozygous females may be bred. Homozygous females are infertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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