Strain Name:

B6;129-Edn2tm1.1Nat/J

Stock Number:

011082

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These mice possess loxP sites on either side of the second exon in the Edn2 (endothelin 2) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in elucidating the many roles of this gene.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Edn2tm1Nat/J    (Changed: 07-APR-10 )
Type Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating Investigator Jeremy Nathans,   Johns Hopkins University

Description
These mice possess loxP sites on either side of the second exon in the Edn2 (endothelin 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed with a Sox2-Cre transgenic strain, mice homozygous for the resulting allele lack Edn2 expression. This strain may be useful in elucidating the many roles of this gene.

Development
The second exon of the targeted gene was flanked by two LoxP sites. Adjacent to the second LoxP site is an Frt site left over after the excision of the PGK-neomycin selectable marker. This mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Edn2tm1.1Nat/Edn2+ Tg(CAG-cre/Esr1*)5Amc/0

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA   (conditional)
  • mortality/aging
  • partial postnatal lethality
    • in mice treated with tamoxifen at P0   (MGI Ref ID J:201436)
  • premature death
    • in mice treated with tamoxifen at P0   (MGI Ref ID J:201436)
    • however, adult mice treated with tamoxifen exhibit normal survival   (MGI Ref ID J:201436)
  • homeostasis/metabolism phenotype
  • abnormal body temperature homeostasis
    • decreased body temperature in response to cold exposure in mice treated with tamoxifen   (MGI Ref ID J:201436)
  • acidemia
    • blood pH is more acidic in tamoxifen-treated mice   (MGI Ref ID J:201436)
  • acidosis
    • blood pH is more acidic in tamoxifen-treated mice   (MGI Ref ID J:201436)
  • decreased body temperature
    • in mice treated with tamoxifen at P0 or 6 weeks of age   (MGI Ref ID J:201436)
  • decreased circulating glucose level
    • in mice treated with tamoxifen at P0 or 6 weeks of age   (MGI Ref ID J:201436)
  • hypercapnia
    • in tamoxifen-treated mice   (MGI Ref ID J:201436)
  • increased circulating bicarbonate level
    • in tamoxifen-treated mice   (MGI Ref ID J:201436)
  • growth/size/body phenotype
  • decreased body weight
    • in mice treated with tamoxifen at P0 or 6 weeks of age   (MGI Ref ID J:201436)
  • decreased total body fat amount
    • after 10 weeks of tamoxifen-induced activation   (MGI Ref ID J:201436)
  • postnatal growth retardation
    • in mice treated with tamoxifen at P0 or 6 weeks of age   (MGI Ref ID J:201436)
  • respiratory system phenotype
  • abnormal pulmonary alveolar sac morphology
    • tamoxifen-treated mice exhibit enlarged air spaces with simplification of the alveolar structure   (MGI Ref ID J:201436)
  • abnormal pulmonary alveolus morphology
    • simplified structure in tamoxifen-treated mice   (MGI Ref ID J:201436)
  • adipose tissue phenotype
  • decreased total body fat amount
    • after 10 weeks of tamoxifen-induced activation   (MGI Ref ID J:201436)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Edn2tm1.1Nat/Edn2tm1.1Nat Tg(Nes-cre)1Kln/0

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL   (conditional)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • mice exhibit normal growth   (MGI Ref ID J:201436)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal core body temperature, even in a cold environment   (MGI Ref ID J:201436)

Edn2tm1.1Nat/Edn2tm1.1Nat Tg(Vil-cre)997Gum/0

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL   (conditional)
  • mortality/aging
  • *normal* mortality/aging
    • mice exhibit normal survival   (MGI Ref ID J:201436)
  • digestive/alimentary phenotype
  • *normal* digestive/alimentary phenotype
    • mice exhibit normal fat and carbohydrate absorption and excretion   (MGI Ref ID J:201436)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • mice exhibit normal growth   (MGI Ref ID J:201436)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal blood glucose levels and body temperature regulation   (MGI Ref ID J:201436)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences
Internal/Organ Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Edn2tm1.1Nat
Allele Name targeted mutation 1.1, Jeremy Nathans
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) ET-2fl;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Edn2, endothelin 2
Chromosome 4
Gene Common Name(s) ET2; PPET2; VIC; vasoactive intestinal contractor peptide;
Molecular Note The second exon of the targeted gene was flanked by two LoxP sites. Adjacent to the second LoxP site is an Frt site left over after the excision of the PGK-neomycin selectable marker. [MGI Ref ID J:158269]

Genotyping

Genotyping Information

Genotyping Protocols

Edn2tm1.1Nat, Standard PCR
Generic Neo Melt Curve Analysis, Melt Curve Analysis


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Edn2tm1.1Nat related

Chang I; Bramall AN; Baynash AG; Rattner A; Rakheja D; Post M; Joza S; McKerlie C; Stewart DJ; McInnes RR; Yanagisawa M. 2013. Endothelin-2 deficiency causes growth retardation, hypothermia, and emphysema in mice. J Clin Invest 123(6):2643-53. [PubMed: 23676500]  [MGI Ref ID J:201436]

Nathans J. 2010. Direct data submission for Edn2<tm1Nat> MGI Direct Data Submission :.  [MGI Ref ID J:158269]

Rattner A; Yu H; Williams J; Smallwood PM; Nathans J. 2013. Endothelin-2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis. Proc Natl Acad Sci U S A 110(40):E3830-E3839. [PubMed: 24043815]  [MGI Ref ID J:201133]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)