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| In this targeted mutation strain, the mouse Olig1 (oligodendrocyte transcription factor 1) locus drives expression of cre in Olig1-expressing cells of the central nervous system (CNS). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
Strain Information
Former Names B6.129S4-Olig1tm1(cre)Rth/J (Changed: 28-FEB-12 ) B6.129S4-Olig1tm1Rth/J (Changed: 15-JAN-10 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 19-JUN-12 Species laboratory mouse Generation F?pN1+F3 (14-FEB-13)
Generation DefinitionsDonating Investigator David H. Rowitch, University of California, San Francisco Description
In this targeted mutation strain, the mouse Olig1 (oligodendrocyte transcription factor 1) locus drives expression of cre in Olig1-expressing cells of the central nervous system (CNS). Olig1 expression is disrupted. When bred with mice containing sequences flanked by similarly oriented loxP sites, flanked sequences will be deleted in the Cre-expressing tissues of the offspring. Homozygotes are viable and reproduce normally.For example, when crossed to a strain with loxP-flanked stop cassette associated with an attentuated diptheria toxin (see Stock No. 010527), this mutant mouse strain may be useful in studies of neural stem cells.
Development
The coding region of the targeted gene was replaced by a cre-neomycin resistance cassette in 129S4/SvJae-derived J1 embryonic stem (ES) cells. This strain was backcrossed six times to C57BL/6 by the donating laboratory (see SNP note below).A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 7 markers on 6 chromosomes were found to be segregating suggesting an incomplete backcross.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (394 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Olig1tm1(cre)Rth/Olig1tm1(cre)Rth
involves: 129S4/SvJae * C57BL/6J
- nervous system phenotype
- abnormal oligodendrocyte morphology
- markers of oligodendrocyte progenitors in the white matter of the spinal cord are delayed, suggesting that maturation of oligodendrocytes in mutants is affected (MGI Ref ID J:75868)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm1(DTA)Mrc/Gt(ROSA)26Sor+ Olig1tm1(cre)Rth/Olig1+
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ (conditional)
- mortality/aging
- complete lethality throughout fetal growth and development
- double heterozygotes are viable to E18.0 but no live mice are recovered (MGI Ref ID J:105927)
- nervous system phenotype
- absent oligodendrocytes
- oligodendrocytes cannot be detected in mutant embryos examined from E12.5-18 (MGI Ref ID J:105927)
- decreased motor neuron number
- at E10.0, few motor neurons are detectable in the ventral spinal cord compared to wild-type; this result is consistent from E10.5-14 (MGI Ref ID J:105927)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Olig1tm1(cre)Rth | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, David H Rowitch | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | CreOlig1 Tg; OLIG1cre; Olig1-; Olig1-cre; Olig1-null;PGKneo; Olig1tm1Rth; | ||
| Strain of Origin | 129S4/SvJae | ||
| Site of Expression | Cre expressed in Olig1-expressing cells of the central nervous system. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Olig1 | ||
| Molecular Note | The majority of the coding region for this gene was replaced with a cre-neo cassette via homologous recombination so that the cre coding sequence is joined in-frame to the endogenous gene's coding region immediately following the translation initiation site. In situ hybridization of spinal cord from homozygous mutant embryos did not detect transcripts from the endogenous gene. In spinal cord from E10.5-E12.5 embryos heterozygous for this allele and for the conditional (Cre recombinase activated) lacZ reporter allele Gt(ROSA)26Sortm1Sor, Xgal staining co-localized with in situ hybridizing mRNAs for motor neuron and oligodendrocyte markers; antibodies to beta-galactosidase co-localized with those to an oligodendrocyte marker, but not to an astrocyte marker. [MGI Ref ID J:75868] | ||
| Gene Symbol and Name | Olig1, oligodendrocyte transcription factor 1 | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | AI836478; AW494459; BHLHB6; BHLHE21; Olg-1; Olg1; expressed sequence AI836478; expressed sequence AW494459; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Olig1tm1(cre)Rthalternate1,Separated MCA
Olig1tm1(cre)Rthalternate1, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lu QR; Sun T; Zhu Z; Ma N; Garcia M; Stiles CD; Rowitch DH. 2002. Common developmental requirement for olig function indicates a motor neuron/oligodendrocyte connection. Cell 109(1):75-86. [PubMed: 11955448] [MGI Ref ID J:75868]
Additional References
Olig1tm1(cre)Rth relatedArnett HA; Fancy SP; Alberta JA; Zhao C; Plant SR; Kaing S; Raine CS; Rowitch DH; Franklin RJ; Stiles CD. 2004. bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS. Science 306(5704):2111-5. [PubMed: 15604411] [MGI Ref ID J:94822]
Benson MD; Romero MI; Lush ME; Lu QR; Henkemeyer M; Parada LF. 2005. Ephrin-B3 is a myelin-based inhibitor of neurite outgrowth. Proc Natl Acad Sci U S A 102(30):10694-9. [PubMed: 16020529] [MGI Ref ID J:100179]
De Biase LM; Kang SH; Baxi EG; Fukaya M; Pucak ML; Mishina M; Calabresi PA; Bergles DE. 2011. NMDA Receptor Signaling in Oligodendrocyte Progenitors Is Not Required for Oligodendrogenesis and Myelination. J Neurosci 31(35):12650-62. [PubMed: 21880926] [MGI Ref ID J:176216]
Fu H; Cai J; Clevers H; Fast E; Gray S; Greenberg R; Jain MK; Ma Q; Qiu M; Rowitch DH; Taylor CM; Stiles CD. 2009. A genome-wide screen for spatially restricted expression patterns identifies transcription factors that regulate glial development. J Neurosci 29(36):11399-408. [PubMed: 19741146] [MGI Ref ID J:152838]
Furusho M; Dupree JL; Nave KA; Bansal R. 2012. Fibroblast growth factor receptor signaling in oligodendrocytes regulates myelin sheath thickness. J Neurosci 32(19):6631-41. [PubMed: 22573685] [MGI Ref ID J:184849]
Guo X; Harada C; Namekata K; Mitamura Y; Yoshida H; Matsumoto Y; Harada T. 2010. Delayed onset of experimental autoimmune encephalomyelitis in Olig1 deficient mice. PLoS One 5(9):. [PubMed: 20927333] [MGI Ref ID J:165093]
Ishii A; Furusho M; Bansal R. 2013. Sustained activation of ERK1/2 MAPK in oligodendrocytes and schwann cells enhances myelin growth and stimulates oligodendrocyte progenitor expansion. J Neurosci 33(1):175-86. [PubMed: 23283332] [MGI Ref ID J:193409]
Ligon KL; Huillard E; Mehta S; Kesari S; Liu H; Alberta JA; Bachoo RM; Kane M; Louis DN; Depinho RA; Anderson DJ; Stiles CD; Rowitch DH. 2007. Olig2-regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma. Neuron 53(4):503-17. [PubMed: 17296553] [MGI Ref ID J:136794]
Ligon KL; Kesari S; Kitada M; Sun T; Arnett HA; Alberta JA; Anderson DJ; Stiles CD; Rowitch DH. 2006. Development of NG2 neural progenitor cells requires Olig gene function. Proc Natl Acad Sci U S A 103(20):7853-8. [PubMed: 16682644] [MGI Ref ID J:110095]
Liu Y; Rao MS. 2004. Olig genes are expressed in a heterogeneous population of precursor cells in the developing spinal cord. Glia 45(1):67-74. [PubMed: 14648547] [MGI Ref ID J:104809]
Mukhopadhyay A; McGuire T; Peng CY; Kessler JA. 2009. Differential effects of BMP signaling on parvalbumin and somatostatin interneuron differentiation. Development 136(15):2633-42. [PubMed: 19592576] [MGI Ref ID J:158140]
Peng CY; Mukhopadhyay A; Jarrett JC; Yoshikawa K; Kessler JA. 2012. BMP receptor 1A regulates development of hypothalamic circuits critical for feeding behavior. J Neurosci 32(48):17211-24. [PubMed: 23197713] [MGI Ref ID J:193055]
Samanta J; Burke GM; McGuire T; Pisarek AJ; Mukhopadhyay A; Mishina Y; Kessler JA. 2007. BMPR1a signaling determines numbers of oligodendrocytes and calbindin-expressing interneurons in the cortex. J Neurosci 27(28):7397-407. [PubMed: 17626200] [MGI Ref ID J:122994]
Toda T; Hayakawa I; Matsubayashi Y; Tanaka K; Ikenaka K; Lu QR; Kawasaki H. 2008. Termination of lesion-induced plasticity in the mouse barrel cortex in the absence of oligodendrocytes. Mol Cell Neurosci 39(1):40-9. [PubMed: 18588982] [MGI Ref ID J:141900]
Virard I; Coquillat D; Bancila M; Kaing S; Durbec P. 2006. Oligodendrocyte precursor cells generate pituicytes in vivo during neurohypophysis development. Glia 53(3):294-303. [PubMed: 16265670] [MGI Ref ID J:156148]
Weng Q; Chen Y; Wang H; Xu X; Yang B; He Q; Shou W; Chen Y; Higashi Y; van den Berghe V; Seuntjens E; Kernie SG; Bukshpun P; Sherr EH; Huylebroeck D; Lu QR. 2012. Dual-mode modulation of Smad signaling by Smad-interacting protein Sip1 is required for myelination in the central nervous system. Neuron 73(4):713-28. [PubMed: 22365546] [MGI Ref ID J:182704]
Wu S; Wu Y; Capecchi MR. 2006. Motoneurons and oligodendrocytes are sequentially generated from neural stem cells but do not appear to share common lineage-restricted progenitors in vivo. Development 133(4):581-90. [PubMed: 16407399] [MGI Ref ID J:105927]
Xin M; Yue T; Ma Z; Wu FF; Gow A; Lu QR. 2005. Myelinogenesis and axonal recognition by oligodendrocytes in brain are uncoupled in Olig1-null mice. J Neurosci 25(6):1354-65. [PubMed: 15703389] [MGI Ref ID J:98102]
Ye F; Chen Y; Hoang T; Montgomery RL; Zhao XH; Bu H; Hu T; Taketo MM; van Es JH; Clevers H; Hsieh J; Bassel-Duby R; Olson EN; Lu QR. 2009. HDAC1 and HDAC2 regulate oligodendrocyte differentiation by disrupting the beta-catenin-TCF interaction. Nat Neurosci 12(7):829-38. [PubMed: 19503085] [MGI Ref ID J:152576]
Yu W; McDonnell K; Taketo MM; Bai CB. 2008. Wnt signaling determines ventral spinal cord cell fates in a time-dependent manner. Development 135(22):3687-96. [PubMed: 18927156] [MGI Ref ID J:143585]
Yu Y; Chen Y; Kim B; Wang H; Zhao C; He X; Liu L; Liu W; Wu LM; Mao M; Chan JR; Wu J; Lu QR. 2013. Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation. Cell 152(1-2):248-61. [PubMed: 23332759] [MGI Ref ID J:193501]
Zeger M; Popken G; Zhang J; Xuan S; Lu QR; Schwab MH; Nave KA; Rowitch D; D'Ercole AJ; Ye P. 2007. Insulin-like growth factor type 1 receptor signaling in the cells of oligodendrocyte lineage is required for normal in vivo oligodendrocyte development and myelination. Glia 55(4):400-11. [PubMed: 17186502] [MGI Ref ID J:156105]
Zhang Y; Argaw AT; Gurfein BT; Zameer A; Snyder BJ; Ge C; Lu QR; Rowitch DH; Raine CS; Brosnan CF; John GR. 2009. Notch1 signaling plays a role in regulating precursor differentiation during CNS remyelination. Proc Natl Acad Sci U S A 106(45):19162-7. [PubMed: 19855010] [MGI Ref ID J:154660]
Zhao S; Hu X; Park J; Zhu Y; Zhu Q; Li H; Luo C; Han R; Cooper N; Qiu M. 2007. Selective expression of LDLR and VLDLR in myelinating oligodendrocytes. Dev Dyn 236(9):2708-12. [PubMed: 17685481] [MGI Ref ID J:124181]
Zhao X; He X; Han X; Yu Y; Ye F; Chen Y; Hoang T; Xu X; Mi QS; Xin M; Wang F; Appel B; Lu QR. 2010. MicroRNA-mediated control of oligodendrocyte differentiation. Neuron 65(5):612-26. [PubMed: 20223198] [MGI Ref ID J:167652]
Zheng K; Li H; Zhu Y; Zhu Q; Qiu M. 2010. MicroRNAs are essential for the developmental switch from neurogenesis to gliogenesis in the developing spinal cord. J Neurosci 30(24):8245-50. [PubMed: 20554876] [MGI Ref ID J:161800]
Zhu Y; Park J; Hu X; Zheng K; Li H; Cao Q; Feng GS; Qiu M. 2010. Control of oligodendrocyte generation and proliferation by Shp2 protein tyrosine phosphatase. Glia 58(12):1407-14. [PubMed: 20648636] [MGI Ref ID J:168041]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes or homozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 19-JUN-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Olig1tm1(cre)Rth
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Olig1tm1(cre)Rth x Homozygous for Olig1tm1(cre)Rth Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Olig1tm1(cre)Rth
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Olig1tm1(cre)Rth x Homozygous for Olig1tm1(cre)Rth Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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