| |||||||||||||||
| These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp1.3) promoter. Cre recombinase expression is detected in epithelial cells of the developing kidney and genitourinary tract and in the renal tubules of adult mice. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Noncarrier (Female x Male) 25-MAY-10 Species laboratory mouse Generation N21+1F3 (11-OCT-11)
Generation DefinitionsDonating Investigator Peter Igarashi, UT Southwestern Medical Center at Dallas Description
Hemizygous and homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter. Cre recombinase expression follows expression of the endogenous gene and is detected in the epithelial cells of developing nephrons, ureteric bud, mesonephric tubules, Wolffian duct, and Mullerian duct. In the adult mouse expression is limited to the renal tubules especially the collecting ducts, loops of Henle and distal tubules. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies.Development
A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter was introduced into ICR fertilized mouse eggs. Founder line 91 was subsequently established and crossed to a C57BL/6 (see SNP note below) for more than 20 generations by the donating laboratory.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (311 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Reproductive Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cdh16-cre)91Igr | ||
|---|---|---|---|
| Allele Name | transgene insertion 91, Peter Igarashi | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Ksp Cre; Ksp-Cre; Ksp1.3-Cre; Ksp1.3/Cre; KspCre; | ||
| Mutation Made By | Peter Igarashi, UT Southwestern Medical Center at Dallas | ||
| Strain of Origin | ICR | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cdh16, cadherin 16, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Cdh16-cre)91Igr, transgene insertion 91, Peter Igarashi | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Ksp-Cre; Ksp1.3-Cre; Ksp1.3/Cre; KspCre; | ||
| Driver Note | Cdh16 | ||
| General Note | This transgenic line is also representative of the cre expression pattern in line 38. | ||
| Molecular Note | The transgene consists of 1341 bp of the Cdh16 5' flanking region, a beta-globin minimal promoter, and a cre recombinase sequence. The Cdh16 promoter was used to drive transgene expression in the developing kidney and genitourinary tract. [MGI Ref ID J:82531] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Cdh16-cre), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Shao X; Somlo S; Igarashi P. 2002. Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract. J Am Soc Nephrol 13(7):1837-46. [PubMed: 12089379] [MGI Ref ID J:82531]
Additional References
Tg(Cdh16-cre)91Igr relatedAdam J; Hatipoglu E; O'Flaherty L; Ternette N; Sahgal N; Lockstone H; Baban D; Nye E; Stamp GW; Wolhuter K; Stevens M; Fischer R; Carmeliet P; Maxwell PH; Pugh CW; Frizzell N; Soga T; Kessler BM; El-Bahrawy M; Ratcliffe PJ; Pollard PJ. 2011. Renal Cyst Formation in Fh1-Deficient Mice Is Independent of the Hif/Phd Pathway: Roles for Fumarate in KEAP1 Succination and Nrf2 Signaling. Cancer Cell 20(4):524-37. [PubMed: 22014577] [MGI Ref ID J:177485]
Baba M; Furihata M; Hong SB; Tessarollo L; Haines DC; Southon E; Patel V; Igarashi P; Alvord WG; Leighty R; Yao M; Bernardo M; Ileva L; Choyke P; Warren MB; Zbar B; Linehan WM; Schmidt LS. 2008. Kidney-targeted Birt-Hogg-Dube gene inactivation in a mouse model: Erk1/2 and Akt-mTOR activation, cell hyperproliferation, and polycystic kidneys. J Natl Cancer Inst 100(2):140-54. [PubMed: 18182616] [MGI Ref ID J:130978]
Bardella C; El-Bahrawy M; Frizzell N; Adam J; Ternette N; Hatipoglu E; Howarth K; O'Flaherty L; Roberts I; Turner G; Taylor J; Giaslakiotis K; Macaulay VM; Harris AL; Chandra A; Lehtonen HJ; Launonen V; Aaltonen LA; Pugh CW; Mihai R; Trudgian D; Kessler B; Baynes JW; Ratcliffe PJ; Tomlinson IP; Pollard PJ. 2011. Aberrant succination of proteins in fumarate hydratase-deficient mice and HLRCC patients is a robust biomarker of mutation status. J Pathol 225(1):4-11. [PubMed: 21630274] [MGI Ref ID J:175252]
Chen J; Futami K; Petillo D; Peng J; Wang P; Knol J; Li Y; Khoo SK; Huang D; Qian CN; Zhao P; Dykyma K; Zhang R; Cao B; Yang XJ; Furge K; Williams BO; Teh BT. 2008. Deficiency of FLCN in mouse kidney led to development of polycystic kidneys and renal neoplasia. PLoS ONE 3(10):e3581. [PubMed: 18974783] [MGI Ref ID J:143922]
Couillard M; Trudel M. 2009. C-myc as a modulator of renal stem/progenitor cell population. Dev Dyn 238(2):405-14. [PubMed: 19161241] [MGI Ref ID J:144178]
Fischer E; Legue E; Doyen A; Nato F; Nicolas JF; Torres V; Yaniv M; Pontoglio M. 2006. Defective planar cell polarity in polycystic kidney disease. Nat Genet 38(1):21-3. [PubMed: 16341222] [MGI Ref ID J:134544]
Frew IJ; Minola A; Georgiev S; Hitz M; Moch H; Richard S; Vortmeyer AO; Krek W. 2008. Combined VHLH and PTEN mutation causes genital tract cystadenoma and squamous metaplasia. Mol Cell Biol 28(14):4536-48. [PubMed: 18474617] [MGI Ref ID J:137442]
Frew IJ; Thoma CR; Georgiev S; Minola A; Hitz M; Montani M; Moch H; Krek W. 2008. pVHL and PTEN tumour suppressor proteins cooperatively suppress kidney cyst formation. EMBO J 27(12):1747-57. [PubMed: 18497742] [MGI Ref ID J:137073]
Goodwin JE; Zhang J; Velazquez H; Geller DS. 2010. The glucocorticoid receptor in the distal nephron is not necessary for the development or maintenance of dexamethasone-induced hypertension. Biochem Biophys Res Commun 394(2):266-71. [PubMed: 20188070] [MGI Ref ID J:158817]
Gresh L; Fischer E; Reimann A; Tanguy M; Garbay S; Shao X; Hiesberger T; Fiette L; Igarashi P; Yaniv M; Pontoglio M. 2004. A transcriptional network in polycystic kidney disease. EMBO J 23(7):1657-68. [PubMed: 15029248] [MGI Ref ID J:131271]
Karner CM; Chirumamilla R; Aoki S; Igarashi P; Wallingford JB; Carroll TJ. 2009. Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. Nat Genet 41(7):793-9. [PubMed: 19543268] [MGI Ref ID J:151498]
Karner CM; Das A; Ma Z; Self M; Chen C; Lum L; Oliver G; Carroll TJ. 2011. Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development. Development 138(7):1247-57. [PubMed: 21350016] [MGI Ref ID J:171512]
Kim D; Wang M; Cai Q; Brooks H; Dressler GR. 2007. Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. J Am Soc Nephrol 18(5):1458-65. [PubMed: 17429055] [MGI Ref ID J:148599]
Kim HY; Verlander JW; Bishop JM; Cain BD; Han KH; Igarashi P; Lee HW; Handlogten ME; Weiner ID. 2009. Basolateral expression of the ammonia transporter family member Rh C glycoprotein in the mouse kidney. Am J Physiol Renal Physiol 296(3):F543-55. [PubMed: 19129254] [MGI Ref ID J:145704]
Landrette SF; Cornett JC; Ni TK; Bosenberg MW; Xu T. 2011. piggyBac transposon somatic mutagenesis with an activated reporter and tracker (PB-SMART) for genetic screens in mice. PLoS One 6(10):e26650. [PubMed: 22039523] [MGI Ref ID J:179725]
Li L; Black R; Ma Z; Yang Q; Wang A; Lin F. 2012. Use of mouse hematopoietic stem and progenitor cells to treat acute kidney injury. Am J Physiol Renal Physiol 302(1):F9-F19. [PubMed: 21937606] [MGI Ref ID J:180011]
Li L; Zepeda-Orozco D; Black R; Lin F. 2010. Autophagy is a component of epithelial cell fate in obstructive uropathy. Am J Pathol 176(4):1767-78. [PubMed: 20150430] [MGI Ref ID J:158607]
Lin F; Hiesberger T; Cordes K; Sinclair AM; Goldstein LS; Somlo S; Igarashi P. 2003. Kidney-specific inactivation of the KIF3A subunit of kinesin-II inhibits renal ciliogenesis and produces polycystic kidney disease. Proc Natl Acad Sci U S A 100(9):5286-91. [PubMed: 12672950] [MGI Ref ID J:83293]
Lin SH; Yu IS; Jiang ST; Lin SW; Chu P; Chen A; Sytwu HK; Sohara E; Uchida S; Sasaki S; Yang SS. 2011. Impaired phosphorylation of Na+-K+-2Cl- cotransporter by oxidative stress-responsive kinase-1 deficiency manifests hypotension and Bartter-like syndrome. Proc Natl Acad Sci U S A 108(42):17538-43. [PubMed: 21972418] [MGI Ref ID J:177443]
Ma Z; Gong Y; Patel V; Karner CM; Fischer E; Hiesberger T; Carroll TJ; Pontoglio M; Igarashi P. 2007. Mutations of HNF-1beta inhibit epithelial morphogenesis through dysregulation of SOCS-3. Proc Natl Acad Sci U S A 104(51):20386-91. [PubMed: 18077349] [MGI Ref ID J:130583]
Parajuli N; Marine A; Simmons S; Saba H; Mitchell T; Shimizu T; Shirasawa T; Macmillan-Crow LA. 2011. Generation and characterization of a novel kidney-specific manganese superoxide dismutase knockout mouse. Free Radic Biol Med 51(2):406-16. [PubMed: 21571061] [MGI Ref ID J:174099]
Pollard PJ; Spencer-Dene B; Shukla D; Howarth K; Nye E; El-Bahrawy M; Deheragoda M; Joannou M; McDonald S; Martin A; Igarashi P; Varsani-Brown S; Rosewell I; Poulsom R; Maxwell P; Stamp GW; Tomlinson IP. 2007. Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. Cancer Cell 11(4):311-9. [PubMed: 17418408] [MGI Ref ID J:120969]
Qian CN; Furge KA; Knol J; Huang D; Chen J; Dykema KJ; Kort EJ; Massie A; Khoo SK; Vanden Beldt K; Resau JH; Anema J; Kahnoski RJ; Morreau H; Camparo P; Comperat E; Sibony M; Denoux Y; Molinie V; Vieillefond A; Eng C; Williams BO; Teh BT. 2009. Activation of the PI3K/AKT pathway induces urothelial carcinoma of the renal pelvis: identification in human tumors and confirmation in animal models. Cancer Res 69(21):8256-64. [PubMed: 19843858] [MGI Ref ID J:153952]
Qian CN; Knol J; Igarashi P; Lin F; Zylstra U; Teh BT; Williams BO. 2005. Cystic Renal Neoplasia Following Conditional Inactivation of Apc in Mouse Renal Tubular Epithelium. J Biol Chem 280(5):3938-3945. [PubMed: 15550389] [MGI Ref ID J:95502]
Saifudeen Z; Dipp S; Stefkova J; Yao X; Lookabaugh S; El-Dahr SS. 2009. p53 regulates metanephric development. J Am Soc Nephrol 20(11):2328-37. [PubMed: 19729440] [MGI Ref ID J:166316]
Shibazaki S; Yu Z; Nishio S; Tian X; Thomson RB; Mitobe M; Louvi A; Velazquez H; Ishibe S; Cantley LG; Igarashi P; Somlo S. 2008. Cyst formation and activation of the extracellular regulated kinase pathway after kidney specific inactivation of Pkd1. Hum Mol Genet 17(11):1505-16. [PubMed: 18263604] [MGI Ref ID J:135301]
Takiar V; Nishio S; Seo-Mayer P; King JD Jr; Li H; Zhang L; Karihaloo A; Hallows KR; Somlo S; Caplan MJ. 2011. Activating AMP-activated protein kinase (AMPK) slows renal cystogenesis. Proc Natl Acad Sci U S A 108(6):2462-7. [PubMed: 21262823] [MGI Ref ID J:169102]
Tiwari S; Sharma N; Gill PS; Igarashi P; Kahn CR; Wade JB; Ecelbarger CM. 2008. Impaired sodium excretion and increased blood pressure in mice with targeted deletion of renal epithelial insulin receptor. Proc Natl Acad Sci U S A 105(17):6469-74. [PubMed: 18424559] [MGI Ref ID J:134541]
Wodarczyk C; Distefano G; Rowe I; Gaetani M; Bricoli B; Muorah M; Spitaleri A; Mannella V; Ricchiuto P; Pema M; Castelli M; Casanova AE; Mollica L; Banzi M; Boca M; Antignac C; Saunier S; Musco G; Boletta A. 2010. Nephrocystin-1 forms a complex with polycystin-1 via a polyproline motif/SH3 domain interaction and regulates the apoptotic response in mammals. PLoS One 5(9):e12719. [PubMed: 20856870] [MGI Ref ID J:165121]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry While maintaining a live colony, these mice are bred as hemizygotes or homozygotes. Mating System Hemizygote x Noncarrier (Female x Male) 25-MAY-10 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
 |
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Cdh16-cre)91Igr
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Cdh16-cre)91Igr x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Cdh16-cre)91Igr Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
|
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Cdh16-cre)91Igr
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Cdh16-cre)91Igr x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Cdh16-cre)91Igr Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.