Strain Name:

B6.129-Aldh1a1tm1Gdu/J

Stock Number:

012247

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Availability:

Cryopreserved - Ready for recovery

This Aldh1a1 (aldehyde dehydrogenase family 1, subfamily A1) targeted mutant lacks exon 11 which encodes the substrate binding pocket and the tetramerization domain. Homozygotes are resistant to diet-induced obesity and insulin resistance and show increased energy dissipation. Approximately 63% of animals are reported to develop mild anterior subcapsular cataracts at 6-9 months of age. This strain may be useful in further characterizing the various functions of the targeted gene.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Sean J Morrison,   UT Southwestern

Description
This Aldh1a1 (aldehyde dehydrogenase family 1, subfamily A1) targeted mutant lacks exon 11 which encodes the substrate binding pocket and the tetramerization domain. Protein is not detected by Western blot analysis of liver, lung and testes. Protein is not detected by immunohistochemistry in embryonic retina. Retinoic acid synthesis is reduced in embryonic retina and adult liver. Expression is significantly reduced in the hematopoietic system, but hematopoiesis is not affected. Homozygotes are resistant to diet-induced obesity and insulin resistance and show increased energy dissipation. Approximately 63% of animals are reported to develop mild anterior subcapsular cataracts at 6-9 months of age. This strain may be useful in further characterizing the various functions of the targeted gene.

Development
Exon 11 was replaced with a PGK-neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for eight generations by the donating laboratory.

Related Strains

View Strains carrying other alleles of neo     (9 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Aldh1a1tm1Gdu/Aldh1a1tm1Gdu

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • liver/biliary system phenotype
  • abnormal hepatobiliary system physiology
    • significantly reduced ability to convert retinol to retinoic acid   (MGI Ref ID J:83957)
  • vision/eye phenotype
  • *normal* vision/eye phenotype
    • in spite of the fact that this gene is highly expressed in the dorsal retina of the mouse, its absence has no apparent effect on retinal morphology   (MGI Ref ID J:83957)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Insulin Resistance
      diet-induced
Obesity Without Diabetes
      diet-induced

Sensorineural Research
Cataracts

neo related

Research Tools
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts), feeder cells for ES cell lines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Aldh1a1tm1Gdu
Allele Name targeted mutation 1, Gregg Duester
Allele Type Targeted (Null/Knockout)
Common Name(s) Raldh1-;
Mutation Made By Gregg Duester,   Burnham Institute for Medical Research
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Expressed Gene neo, neomycin resistance, bacterial
Molecular Note Exon 11 was replaced by a PGK-neomycin gene. This results in eliminating the region containing the substrate binding pocket and the tetramerization domain in any mRNA that might be transcribed. Resulting mice were genotyped by Southern blots. Absence of protein product was established by Western blot analysis. [MGI Ref ID J:83957]
 
Gene Symbol and Name Aldh1a1, aldehyde dehydrogenase family 1, subfamily A1
Chromosome 19
Gene Common Name(s) ALDC; ALDH-E1; ALDH1; ALDH11; Ahd-2; Ahd2; Aldh1; Aldh2; E1; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1; aldehyde dehydrogenase 1, liver cytosolic (class 1); aldehyde dehydrogenase 2, cytoplasmic;

Genotyping

Genotyping Information

Genotyping Protocols

Aldh1a1tm1Gdu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Fan X; Molotkov A; Manabe S; Donmoyer CM; Deltour L; Foglio MH; Cuenca AE; Blaner WS; Lipton SA; Duester G. 2003. Targeted disruption of Aldh1a1 (Raldh1) provides evidence for a complex mechanism of retinoic acid synthesis in the developing retina. Mol Cell Biol 23(13):4637-48. [PubMed: 12808103]  [MGI Ref ID J:83957]

Additional References

Aldh1a1tm1Gdu related

Anderson DW; Schray RC; Duester G; Schneider JS. 2011. Functional significance of aldehyde dehydrogenase ALDH1A1 to the nigrostriatal dopamine system. Brain Res 1408:81-7. [PubMed: 21784415]  [MGI Ref ID J:175780]

Gasparetto M; Sekulovic S; Brocker C; Tang P; Zakaryan A; Xiang P; Kuchenbauer F; Wen M; Kasaian K; Witty MF; Rosten P; Chen Y; Imren S; Duester G; Thompson DC; Humphries RK; Vasiliou V; Smith C. 2012. Aldehyde dehydrogenases are regulators of hematopoietic stem cell numbers and B-cell development. Exp Hematol 40(4):318-29.e2. [PubMed: 22198153]  [MGI Ref ID J:191504]

Goldstein DS; Sullivan P; Holmes C; Miller GW; Alter S; Strong R; Mash DC; Kopin IJ; Sharabi Y. 2013. Determinants of buildup of the toxic dopamine metabolite DOPAL in Parkinson's disease. J Neurochem 126(5):591-603. [PubMed: 23786406]  [MGI Ref ID J:200778]

Gushchina LV; Yasmeen R; Ziouzenkova O. 2013. Moderate vitamin A supplementation in obese mice regulates tissue factor and cytokine production in a sex-specific manner. Arch Biochem Biophys 539(2):239-47. [PubMed: 23850584]  [MGI Ref ID J:206684]

Kiefer FW; Orasanu G; Nallamshetty S; Brown JD; Wang H; Luger P; Qi NR; Burant CF; Duester G; Plutzky J. 2012. Retinaldehyde dehydrogenase 1 coordinates hepatic gluconeogenesis and lipid metabolism. Endocrinology 153(7):3089-99. [PubMed: 22555438]  [MGI Ref ID J:189058]

Kiefer FW; Vernochet C; O'Brien P; Spoerl S; Brown JD; Nallamshetty S; Zeyda M; Stulnig TM; Cohen DE; Kahn CR; Plutzky J. 2012. Retinaldehyde dehydrogenase 1 regulates a thermogenic program in white adipose tissue. Nat Med 18(6):918-25. [PubMed: 22561685]  [MGI Ref ID J:187531]

Kumar S; Duester G. 2010. Retinoic acid signaling in perioptic mesenchyme represses Wnt signaling via induction of Pitx2 and Dkk2. Dev Biol 340(1):67-74. [PubMed: 20122913]  [MGI Ref ID J:158502]

Lassen N; Bateman JB; Estey T; Kuszak JR; Nees DW; Piatigorsky J; Duester G; Day BJ; Huang J; Hines LM; Vasiliou V. 2007. Multiple and Additive Functions of ALDH3A1 and ALDH1A1: CATARACT PHENOTYPE AND OCULAR OXIDATIVE DAMAGE IN Aldh3a1(-/-)/Aldh1a1(-/-) KNOCK-OUT MICE. J Biol Chem 282(35):25668-76. [PubMed: 17567582]  [MGI Ref ID J:124711]

Levi BP; Yilmaz OH; Duester G; Morrison SJ. 2009. Aldehyde dehydrogenase 1a1 is dispensable for stem cell function in the mouse hematopoietic and nervous systems. Blood 113(8):1670-80. [PubMed: 18971422]  [MGI Ref ID J:145577]

Mcilroy GD; Delibegovic M; Owen C; Stoney PN; Shearer KD; McCaffery PJ; Mody N. 2013. Fenretinide treatment prevents diet-induced obesity in association with major alterations in retinoid homeostatic gene expression in adipose, liver, and hypothalamus. Diabetes 62(3):825-36. [PubMed: 23193184]  [MGI Ref ID J:208599]

Mic FA; Molotkov A; Molotkova N; Duester G. 2004. Raldh2 expression in optic vesicle generates a retinoic acid signal needed for invagination of retina during optic cup formation. Dev Dyn 231(2):270-7. [PubMed: 15366004]  [MGI Ref ID J:93091]

Molotkov A; Duester G. 2003. Genetic evidence that retinaldehyde dehydrogenase Raldh1 (Aldh1a1) functions downstream of alcohol dehydrogenase Adh1 in metabolism of retinol to retinoic acid. J Biol Chem 278(38):36085-90. [PubMed: 12851412]  [MGI Ref ID J:85538]

Molotkov A; Molotkova N; Duester G. 2006. Retinoic acid guides eye morphogenetic movements via paracrine signaling but is unnecessary for retinal dorsoventral patterning. Development 133(10):1901-10. [PubMed: 16611695]  [MGI Ref ID J:108515]

Nallamshetty S; Wang H; Rhee EJ; Kiefer FW; Brown JD; Lotinun S; Le P; Baron R; Rosen CJ; Plutzky J. 2013. Deficiency of retinaldehyde dehydrogenase 1 induces BMP2 and increases bone mass in vivo. PLoS One 8(8):e71307. [PubMed: 23951127]  [MGI Ref ID J:205888]

Wright-Jin EC; Grider JR; Duester G; Heuckeroth RO. 2013. Retinaldehyde dehydrogenase enzymes regulate colon enteric nervous system structure and function. Dev Biol 381(1):28-37. [PubMed: 23806210]  [MGI Ref ID J:200936]

Yasmeen R; Meyers JM; Alvarez CE; Thomas JL; Bonnegarde-Bernard A; Alder H; Papenfuss TL; Benson DM Jr; Boyaka PN; Ziouzenkova O. 2013. Aldehyde dehydrogenase-1a1 induces oncogene suppressor genes in B cell populations. Biochim Biophys Acta 1833(12):3218-27. [PubMed: 24080087]  [MGI Ref ID J:204057]

Yasmeen R; Reichert B; Deiuliis J; Yang F; Lynch A; Meyers J; Sharlach M; Shin S; Volz KS; Green KB; Lee K; Alder H; Duester G; Zechner R; Rajagopalan S; Ziouzenkova O. 2013. Autocrine function of aldehyde dehydrogenase 1 as a determinant of diet- and sex-specific differences in visceral adiposity. Diabetes 62(1):124-36. [PubMed: 22933113]  [MGI Ref ID J:208490]

Ziouzenkova O; Orasanu G; Sharlach M; Akiyama TE; Berger JP; Viereck J; Hamilton JA; Tang G; Dolnikowski GG; Vogel S; Duester G; Plutzky J. 2007. Retinaldehyde represses adipogenesis and diet-induced obesity. Nat Med 13(6):695-702. [PubMed: 17529981]  [MGI Ref ID J:121870]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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