Strain Name:

B6;129-Terf2iptm1.1Tdl/J

Stock Number:

012346

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Rap1F mice harbor loxP sites flanking exon 2 (encoding the TRF2 binding domain) of the mouse Rap1 locus (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2 interacting protein). Telomeres serve a dual role in protecting the chromosome ends from degradation/repair activities and in intracellular signaling for regulating cell proliferation. Mammalian telomeres are formed by tandem TTAGGG sequence repeats bound by a specialized complex of six telomere-associated proteins called the shelterin complex. As Rap1 is one of the components of shelterin, these Rap1F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating Investigator Titia de Lange,   The Rockefeller University

Description
Mice homozygous for this Rap1F allele are viable and fertile, with loxP sites flanking exon 2 of the mouse Rap1 gene (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2 interacting protein). When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the TRF2 binding domain deleted in the cre-expressing tissues. As Rap1 is one of the components of shelterin, these Rap1F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging.

Development
A targeting vector was designed to insert a frt-flanked neomycin cassette and loxP site upstream of exon 2, and a second loxP site (together with an NdeI site) downstream of exon 2 of the mouse Rap1 locus (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2, interacting protein). The construct was electroporated into CY2.4 embryonic stem (ES) cells derived from C57BL/6J-Tyrc-2J (aka B6(Cg)-Tyrc-2J/J ; Stock No. 000058). Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with albino C57BL/6J-Tyrc-2J females to establish the colony. Rap1 mutant mice were then bred to FLPe-deleter mice (B6;129 mixed genetic background; derived from Stock No. 003946) to remove the frt-flanked neo cassette. The resulting Rap1F mice (with loxP site and single frt site remaining upstream of exon 2, and a second loxP site downstream of exon 2) were selected. Black Rap1F mice were bred together (and the FLPe-expressing transgene was removed) for many generations, and then backcrossed at least one generation to C57BL/6J mice prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. These Rap1F mice may still be segregating for the Tyrc-2J mutation.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Terf2iptm1.1Tdl/Terf2ip+

        involves: C57BL/6J
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:158884)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Other
      DNA Repair

Cell Biology Research
DNA Damage Response
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cancer Research
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Terf2iptm1.1Tdl
Allele Name targeted mutation 1.1, Titia de Lange
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Rap1F;
Mutation Made By Titia de Lange,   The Rockefeller University
Strain of OriginB6(Cg)-Tyr
Gene Symbol and Name Terf2ip, telomeric repeat binding factor 2, interacting protein
Chromosome 8
Gene Common Name(s) DRIP5; RAP1;
Molecular Note An frt flanked neo cassette with a 3' loxP site was inserted upstream of exon 2 and an additional loxP site was inserted downstream of exon 2. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. [MGI Ref ID J:158884]

Genotyping

Genotyping Information

Genotyping Protocols

Terf2iptm1.1Tdl, Melt Curve Analysis
Terf2iptm1.1Tdl, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sfeir A; Kabir S; van Overbeek M; Celli GB; de Lange T. 2010. Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Science 327(5973):1657-61. [PubMed: 20339076]  [MGI Ref ID J:158884]

Additional References

Terf2iptm1.1Tdl related

Martinez P; Gomez-Lopez G; Garcia F; Mercken E; Mitchell S; Flores JM; de Cabo R; Blasco MA. 2013. RAP1 protects from obesity through its extratelomeric role regulating gene expression. Cell Rep 3(6):2059-74. [PubMed: 23791526]  [MGI Ref ID J:199371]

Yeung F; Ramirez CM; Mateos-Gomez PA; Pinzaru A; Ceccarini G; Kabir S; Fernandez-Hernando C; Sfeir A. 2013. Nontelomeric role for Rap1 in regulating metabolism and protecting against obesity. Cell Rep 3(6):1847-56. [PubMed: 23791522]  [MGI Ref ID J:199372]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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