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| The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ppard (peroxisome proliferator activator receptor delta) in these transgenic mice. Hemizygotes have increased myocardial glucose utilization, do not accumulate myocardial lipid, and have normal cardiac function. Expression levels in this "high expression" (HE) line are increased approximately 100-fold over endogenous levels. This strain may be useful in studies of cardiac function and metabolic modulation during diabetes and ischemia. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N7pN1
Generation DefinitionsDonating Investigator Daniel Kelly, Burnham Institute for Medical Research Description
The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ppard (peroxisome proliferator activator receptor delta) in these transgenic mice. Hemizygotes have increased myocardial glucose utilization, do not accumulate myocardial lipid, and have normal cardiac function. Expression levels in this "high expression" (HE) line are increased approximately 100-fold over endogenous levels. This strain may be useful in studies of cardiac function and metabolic modulation during diabetes and ischemia.Development
A mouse Ppard cDNA was cloned downstream of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The transgenic construct was microinjected into B6CBAF1 embryos. This mixed genetic background was maintained by the donating laboratory by crossing offspring to B6CBAF1. These mice represent the "high expression" line described in the original reference.
Related Strains
Strains carrying other alleles of Myh6
View Strains carrying other alleles of Myh6 (38 strains)
005828 B6.129P2-Ppardtm1Dgen/J 005897 B6.129S4-Ppardtm1Rev/J 005901 B6.129S4-Ppardtm2Rev/J
Phenotype
Phenotype Information
assigned by genotype
Tg(Myh6-Ppard)HEDpk/0
involves: C57BL/6 * CBA
- cardiovascular system phenotype
- *normal* cardiovascular system phenotype
- mice exhibit normal ventricular weight and cardiac response to a high fat diet (MGI Ref ID J:130750)
- abnormal cardiac cell glucose uptake
- increased (MGI Ref ID J:130750)
- altered response to myocardial infarction
- mice exhibit reduced ischemia/reperfusion injury compared with wild-type mice (MGI Ref ID J:130750)
- decreased infarction size
- following ischemia/reperfusion injury, infarct area relative to area at risk is decreased compared to in similarly treated wild-type mice (MGI Ref ID J:130750)
- decreased cardiac muscle contractility
- without ventricular hypertrophy (MGI Ref ID J:130750)
- homeostasis/metabolism phenotype
- altered response to myocardial infarction
- mice exhibit reduced ischemia/reperfusion injury compared with wild-type mice (MGI Ref ID J:130750)
- decreased infarction size
- following ischemia/reperfusion injury, infarct area relative to area at risk is decreased compared to in similarly treated wild-type mice (MGI Ref ID J:130750)
- increased glycogen level
- in myocardial cells (MGI Ref ID J:130750)
- muscle phenotype
- abnormal cardiac cell glucose uptake
- increased (MGI Ref ID J:130750)
- decreased cardiac muscle contractility
- without ventricular hypertrophy (MGI Ref ID J:130750)
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Internal/Organ Research
Heart Abnormalities
Metabolism Research
Lipid Metabolism
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Myh6-Ppard)HEDpk | ||
|---|---|---|---|
| Allele Name | transgene insertion HE, Daniel P Kelly | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | MHC-PPARbeta; | ||
| Mutation Made By | Teresa Leone, Burnham Institute for Medical Research | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Expressed Gene | Ppard, peroxisome proliferator activator receptor delta, mouse, laboratory | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine | ||
| Molecular Note | A mouse Ppard cDNA was cloned downstream of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. Three lines were created with low (20-fold over levels of the corresponding nuclear receptor), medium (50-fold), and high (100-fold)expression levels (LE, ME, and HE). This is a high expression line. [MGI Ref ID J:130750] | ||
References
References provided by MGI
Selected Reference(s)
Burkart EM; Sambandam N; Han X; Gross RW; Courtois M; Gierasch CM; Shoghi K; Welch MJ; Kelly DP. 2007. Nuclear receptors PPARbeta/delta and PPARalpha direct distinct metabolic regulatory programs in the mouse heart. J Clin Invest 117(12):3930-9. [PubMed: 18037994] [MGI Ref ID J:130750]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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