Strain Name:

B6CBA-Tg(Myh6-Ppard)HEDpk/J

Stock Number:

012383

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Availability:

Cryopreserved - Ready for recovery

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The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ppard (peroxisome proliferator activator receptor delta) in these transgenic mice. Hemizygotes have increased myocardial glucose utilization, do not accumulate myocardial lipid, and have normal cardiac function. Expression levels in this "high expression" (HE) line are increased approximately 100-fold over endogenous levels. This strain may be useful in studies of cardiac function and metabolic modulation during diabetes and ischemia.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN7pN1
Generation Definitions
 
Donating Investigator Daniel Kelly,   Burnham Institute for Medical Research

Description
The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ppard (peroxisome proliferator activator receptor delta) in these transgenic mice. Hemizygotes have increased myocardial glucose utilization, do not accumulate myocardial lipid, and have normal cardiac function. Expression levels in this "high expression" (HE) line are increased approximately 100-fold over endogenous levels. This strain may be useful in studies of cardiac function and metabolic modulation during diabetes and ischemia.

Development
A mouse Ppard cDNA was cloned downstream of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The transgenic construct was microinjected into B6CBAF1 embryos. This mixed genetic background was maintained by the donating laboratory by crossing offspring to B6CBAF1. These mice represent the "high expression" line described in the original reference.

Related Strains

Strains carrying other alleles of Myh6
012389   B6.Cg-Tg(Myh6-Ppara)404-3Dpk/J
012382   B6.Cg-Tg(Myh6-Ppara)404-4Dpk/J
005657   B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J
011038   B6.FVB-Tg(Myh6-cre)2182Mds/J
013781   B6;FVB-Tg(Myh6/NFAT-luc)1Jmol/J
002640   B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
018972   B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J
002639   B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
002638   B6SJL-Tg(WTbeta2)4Wjk/J
006768   D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ
010587   FVB-Tg(Myh6-MEF2A)1Jmol/J
010581   FVB-Tg(Myh6-Map2k1*)1Jmol/J
010582   FVB-Tg(Myh6-Map2k3*)1Jmol/J
010584   FVB-Tg(Myh6-Map2k7)1Jmol/J
010585   FVB-Tg(Myh6-Mapk1)1Jmol/J
010583   FVB-Tg(Myh6-Mapk14*)1Jmol/J
010586   FVB-Tg(Myh6-Mef2c)2Jmol/J
009438   FVB-Tg(Myh6-SOD2,Tyr)3Pne/J
016570   FVB-Tg(Myh6-TRPC3*)6.6Jmol/J
017543   FVB-Tg(Myh6-TRPC6*)1Jmol/J
011037   FVB-Tg(Myh6-cre)2182Mds/J
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
014153   FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J
012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
010580   FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J
022979   FVB-Tg(tetO-Thbs4)17.7Jmol/J
002535   FVB/N-Tg(ANX6)2Agh/J
012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
008716   FVB/N-Tg(Myh6-AIP/PLN*)46Jded/J
012461   FVB/N-Tg(Myh6-Cast)1Gwd/J
012460   FVB/N-Tg(Myh6-Gnaq)40Gwd/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
009075   STOCK Tg(Myh6-Ppp3ca)37Eno/J
010579   STOCK Tg(Myh6-Prkca)1Jmk/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
005650   STOCK Tg(Myh6-cre/Esr1*)1Jmk/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
View Strains carrying other alleles of Myh6     (40 strains)

Strains carrying other alleles of Ppard
005828   B6.129P2-Ppardtm1Dgen/J
005897   B6.129S4-Ppardtm1Rev/J
005901   B6.129S4-Ppardtm2Rev/J
View Strains carrying other alleles of Ppard     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Myh6-Ppard)HEDpk/0

        involves: C57BL/6 * CBA
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • mice exhibit normal ventricular weight and cardiac response to a high fat diet   (MGI Ref ID J:130750)
    • abnormal cardiac cell glucose uptake
    • altered response to myocardial infarction
      • mice exhibit reduced ischemia/reperfusion injury compared with wild-type mice   (MGI Ref ID J:130750)
      • decreased myocardial infarction size
        • following ischemia/reperfusion injury, infarct area relative to area at risk is decreased compared to in similarly treated wild-type mice   (MGI Ref ID J:130750)
    • decreased cardiac muscle contractility
      • without ventricular hypertrophy   (MGI Ref ID J:130750)
  • homeostasis/metabolism phenotype
  • altered response to myocardial infarction
    • mice exhibit reduced ischemia/reperfusion injury compared with wild-type mice   (MGI Ref ID J:130750)
    • decreased myocardial infarction size
      • following ischemia/reperfusion injury, infarct area relative to area at risk is decreased compared to in similarly treated wild-type mice   (MGI Ref ID J:130750)
  • increased glycogen level
    • in myocardial cells   (MGI Ref ID J:130750)
  • muscle phenotype
  • abnormal cardiac cell glucose uptake
  • decreased cardiac muscle contractility
    • without ventricular hypertrophy   (MGI Ref ID J:130750)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research

Internal/Organ Research
Heart Abnormalities

Metabolism Research
Lipid Metabolism

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Myh6-Ppard)HEDpk
Allele Name transgene insertion HE, Daniel P Kelly
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) MHC-PPARbeta;
Mutation Made By Teresa Leone,   Burnham Institute for Medical Research
Strain of Origin(C57BL/6 x CBA)F1
Expressed Gene Ppard, peroxisome proliferator activator receptor delta, mouse, laboratory
Promoter Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine
Molecular Note A mouse Ppard cDNA was cloned downstream of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. Three lines were created with low (20-fold over levels of the corresponding nuclear receptor), medium (50-fold), and high (100-fold)expression levels (LE, ME, and HE). This is a high expression line. [MGI Ref ID J:130750]
 
 

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Burkart EM; Sambandam N; Han X; Gross RW; Courtois M; Gierasch CM; Shoghi K; Welch MJ; Kelly DP. 2007. Nuclear receptors PPARbeta/delta and PPARalpha direct distinct metabolic regulatory programs in the mouse heart. J Clin Invest 117(12):3930-9. [PubMed: 18037994]  [MGI Ref ID J:130750]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, hemizygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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