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| These mice carry a floxed allele of Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta). When bred to mice with a cre recombinase gene under the control of a promoter of interest, exons 4-6 of the targeted gene are deleted in the tissue of interest. This strain may be useful in studies of the metabolic and functional maturation in the tissue of interest, using a conditional "knockout" strategy. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N4pN1
Generation DefinitionsDonating Investigator Daniel Kelly, Burnham Institute for Medical Research Description
These mice carry a floxed allele of Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta). When bred to mice with a cre recombinase gene under the control of a promoter of interest, exons 4-6 of the targeted gene are deleted in the tissue of interest. This strain may be useful in studies of the metabolic and functional maturation in the tissue of interest, using a conditional "knockout" strategy.Development
A loxP-flanked neomycin resistance cassette was introduced into intron 3 and a third loxP site was placed in intron 6 of the targeted gene in 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Resultant mice were backcrossed 3 times to C57BL/6 by the donating laboratory.
Related Strains
Strains carrying other alleles of Ppargc1b
008737 FVB-Tg(Ckm-Ppargc1b)T37Brsp/J 012385 FVB-Tg(tetO-Ppargc1b)7Dpk/J View Strains carrying other alleles of Ppargc1b (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Obesity (PPARGC1B)
This mouse can be used to support research in many areas including:
Research Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
loxP-flanked Sequences
Metabolism Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ppargc1btm1Dpk | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Daniel P Kelly | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | PGC-1betaf; | ||
| Mutation Made By | Teresa Leone, Burnham Institute for Medical Research | ||
| Strain of Origin | 129X1/SvJ | ||
| Gene Symbol and Name | Ppargc1b, peroxisome proliferative activated receptor, gamma, coactivator 1 beta | ||
| Chromosome | 18 | ||
| Gene Common Name(s) | 4631412G21Rik; ERRL1; PERC; PGC-1(beta); PGC-1beta/ERRL1; PGC1B; RIKEN cDNA 4631412G21 gene; | ||
| Molecular Note | A floxed neo cassette was inserted downstream of exon 3 and an additional loxP site was inserted downstream of exon 6. Germ line, cre-mediated recombination was used to remove the neo cassette leaving exons 4 through 6 floxed. [MGI Ref ID J:137598] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ppargc1btm1Dpk, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lai L; Leone TC; Zechner C; Schaeffer PJ; Kelly SM; Flanagan DP; Medeiros DM; Kovacs A; Kelly DP. 2008. Transcriptional coactivators PGC-1{alpha} and PGC-l{beta} control overlapping programs required for perinatal maturation of the heart. Genes Dev 22(14):1948-61. [PubMed: 18628400] [MGI Ref ID J:137598]
Additional References
Ppargc1btm1Dpk relatedChambers KT; Chen Z; Crawford PA; Fu X; Burgess SC; Lai L; Leone TC; Kelly DP; Finck BN. 2012. Liver-specific PGC-1beta deficiency leads to impaired mitochondrial function and lipogenic response to fasting-refeeding. PLoS One 7(12):e52645. [PubMed: 23285128] [MGI Ref ID J:195754]
Shoag J; Haq R; Zhang M; Liu L; Rowe GC; Jiang A; Koulisis N; Farrel C; Amos CI; Wei Q; Lee JE; Zhang J; Kupper TS; Qureshi AA; Cui R; Han J; Fisher DE; Arany Z. 2013. PGC-1 coactivators regulate MITF and the tanning response. Mol Cell 49(1):145-57. [PubMed: 23201126] [MGI Ref ID J:194191]
Zechner C; Lai L; Zechner JF; Geng T; Yan Z; Rumsey JW; Collia D; Chen Z; Wozniak DF; Leone TC; Kelly DP. 2010. Total skeletal muscle PGC-1 deficiency uncouples mitochondrial derangements from fiber type determination and insulin sensitivity. Cell Metab 12(6):633-42. [PubMed: 21109195] [MGI Ref ID J:168114]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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