Strain Name:

NOD.B10Sn-Idd5.1C57BL/10SnJ/R46MrkTacJ

Stock Number:

012391

Order this mouse

Availability:

Cryopreserved - Ready for recovery

This strain is one of a set of eight NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 004344,012392, 012393, 012394, and 012395 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NOD.B10Sn-Idd5aC57BL/10SnJ/R46MrkTacJ    (Changed: 17-MAY-10 )
Type Congenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain NOD/ShiLtJ
Donor Strain C57BL/10SnJ
H2 Haplotypeg7
GenerationN16F?+F6pN1
Generation Definitions
 
Donating Investigator Linda Wicker,   University of Cambridge, UK

Description
This NOD congenic strain, commonly referred to as NOD.B10-Idd5R46, line 2193, carries a diabetes resistant C57BL/10SnJ (Stock No. 000666) chromosome 1 segment. The B10 introgressed segment is defined within D1Mit74 (57.6Mb) through AL671560 (SNP marker at approximately 62Mb) and includes the insulin dependent diabetes susceptibility (Idd)5.1; including candidate gene Ctla4. Diabetes incidence is modest but significant among 7 month old female mice of this strain, which is reported at 62%, compared with more than 80% in NOD controls (Hunter, et al. 2007).

This strain may be useful to understand the epistatic interactions of Idd5.1 alleles with other Idd loci.

Development
This NOD/MrkTac congenic stock contains a genomic segment, D1Mit74 (57.6Mb) through AL671560 (SNP marker at approximately 62Mb), from C57BL/10SnJ (B10) introgressed into chromosome 1. The B10 genomic segment includes Idd5.1. The major candidate gene for Idd5.1 is Ctla4. This strain was produced by mating mice from NOD/MrkTac (NOD) to B10 followed by 15 additional crosses to NOD with selection for chromosome 1, Idd5 markers. Siblings carrying the B10 derived segments denoted by markers D1Mit74 through AL671560 were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 37, identifies no B10 genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Idd5.1C57BL/10SnJ allele
012392   NOD.B10Sn-Idd5C57Bl/10SnJ/R193MrkTacJ
View Strains carrying   Idd5.1C57BL/10SnJ     (1 strain)

Strains carrying other alleles of Idd5.1
008894   NOD.B10Sn-Idd5C57BL/10SnJ/974MrkJ
View Strains carrying other alleles of Idd5.1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Idd5.1C57BL/10SnJ/Idd5.1C57BL/10SnJ

        involves: C57BL/10SnJ * NOD/MrkTac
  • homeostasis/metabolism phenotype
  • abnormal glucose homeostasis
    • resistance to type 1 diabetes   (MGI Ref ID J:88667)
    • resistance to insulin dependent diabetes   (MGI Ref ID J:90924)
    • resistance to type 1 diabetes   (MGI Ref ID J:143549)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
      NOD/ShiLtJ Non-MHC Congenics

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      Type 1 Diabetes

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Idd5.1C57BL/10SnJ
Allele Name C57BL/10SnJ
Allele Type QTL
Common Name(s) Idd5.1;
Strain of OriginC57BL/10SnJ
Gene Symbol and Name Idd5.1, insulin dependent diabetes susceptibility 5.1
Chromosome 1
Gene Common Name(s) Idd5a; insulin dependent diabetes susceptibility 5a;
Molecular Note This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac. [MGI Ref ID J:88667]

Genotyping

Genotyping Information

Genotyping Protocols

D1Mit320, Standard PCR
D1Mit74, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hunter K; Rainbow D; Plagnol V; Todd JA; Peterson LB; Wicker LS. 2007. Interactions between Idd5.1/Ctla4 and other type 1 diabetes genes. J Immunol 179(12):8341-9. [PubMed: 18056379]  [MGI Ref ID J:143549]

Additional References

Idd5.1C57BL/10SnJ related

Araki M; Chung D; Liu S; Rainbow DB; Chamberlain G; Garner V; Hunter KM; Vijayakrishnan L; Peterson LB; Oukka M; Sharpe AH; Sobel R; Kuchroo VK; Wicker LS. 2009. Genetic evidence that the differential expression of the ligand-independent isoform of CTLA-4 is the molecular basis of the Idd5.1 type 1 diabetes region in nonobese diabetic mice. J Immunol 183(8):5146-57. [PubMed: 19783679]  [MGI Ref ID J:153599]

Dai YD; Marrero IG; Gros P; Zaghouani H; Wicker LS; Sercarz EE. 2009. Slc11a1 enhances the autoimmune diabetogenic T-cell response by altering processing and presentation of pancreatic islet antigens. Diabetes 58(1):156-64. [PubMed: 18984740]  [MGI Ref ID J:146983]

Hamilton-Williams EE; Cheung J; Rainbow DB; Hunter KM; Wicker LS; Sherman LA. 2012. Cellular mechanisms of restored beta-cell tolerance mediated by protective alleles of Idd3 and Idd5. Diabetes 61(1):166-74. [PubMed: 22106155]  [MGI Ref ID J:191380]

Hill NJ; Lyons PA; Armitage N; Todd JA; Wicker LS; Peterson LB. 2000. NOD Idd5 locus controls insulitis and diabetes and overlaps the orthologous CTLA4/IDDM12 and NRAMP1 loci in humans. Diabetes 49(10):1744-7. [PubMed: 11016460]  [MGI Ref ID J:88667]

Kochupurakkal NM; Kruger AJ; Tripathi S; Zhu B; Adams LT; Rainbow DB; Rossini A; Greiner DL; Sayegh MH; Wicker LS; Guleria I. 2014. Blockade of the programmed death-1 (PD1) pathway undermines potent genetic protection from type 1 diabetes. PLoS One 9(2):e89561. [PubMed: 24586872]  [MGI Ref ID J:213816]

Lin X; Hamilton-Williams EE; Rainbow DB; Hunter KM; Dai YD; Cheung J; Peterson LB; Wicker LS; Sherman LA. 2013. Genetic Interactions among Idd3, Idd5.1, Idd5.2, and Idd5.3 Protective Loci in the Nonobese Diabetic Mouse Model of Type 1 Diabetes. J Immunol 190(7):3109-20. [PubMed: 23427248]  [MGI Ref ID J:194526]

Wicker LS; Chamberlain G; Hunter K; Rainbow D; Howlett S; Tiffen P; Clark J; Gonzalez-Munoz A; Cumiskey AM; Rosa RL; Howson JM; Smink LJ; Kingsnorth A; Lyons PA; Gregory S; Rogers J; Todd JA; Peterson LB. 2004. Fine mapping, gene content, comparative sequencing, and expression analyses support Ctla4 and Nramp1 as candidates for Idd5.1 and Idd5.2 in the nonobese diabetic mouse. J Immunol 173(1):164-73. [PubMed: 15210771]  [MGI Ref ID J:90924]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)