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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129S6.129X1(B6)-Syt4tm1Hrh/J (Changed: 18-JUN-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7+F2pN1
Generation DefinitionsDonating Investigator Edwin R Chapman, University of Wisconsin, Madison Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of total brain RNA. Homozygotes exhibit impaired locomotor coordination (reduced performance on accelerating rotarod), diminished contextual fear conditioning, impaired social transmission of food preference, enhanced long-term potentiation (LTP) and hyperactivity. Anxiety-like behavior and depression-like behavior is decreased in homozygous animals. Posterior pituitary nerve terminals isolated from homozygotes exhibit lower Ca2+ current density than wildtype controls, decreased exocytosis and accelerated endocytosis with high Ca2+ entry, and increased exocytosis with low Ca2+ entry. Hippocampal neurons isolated from homozygotes display increased rate of synpatic vesicle exocytosis from presynaptic terminals. Inner hair cells from homozygotes have abnormal exocytotic Ca2+ dependence. Mice that are homozygous for this targeted mutation may be useful in studies of learning, behavior and synaptic function and plasticity.Development
A targeting vector containing a Neo cassette and herpes simplex virus thymidine kinase gene was used to disrupt an exon which encodes the first C2 domain. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to C57BL/6 female mice. The mice were backcrossed to C57BL/6 for several generations, then backcrossed to 129S6/SvEvTac for 7 or 8 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mutant females and mutant males were bred together to establish the colony.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Markers on Chromosomes 8 and 13 are segregating, suggesting an incomplete backcross.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Syt4tm1Hahe/Syt4tm1Hahe
involves: 129X1/SvJ * C57BL/6
- behavior/neurological phenotype
- abnormal contextual conditioning behavior
- in a foot shock test, homozygotes exhibited a deficit in freezing 24 hours after training compared to controls (MGI Ref ID J:62218)
- impaired coordination
- homozygous mice exhibit an impaired ability to remain on an accelerating rotarod compared to controls (MGI Ref ID J:62218)
- impaired social transmission of food preference
- homozygous mice exhibit no preference for cued foods 24 hours after interaction with demonstrators; however, homozygotes did exhibit a preference immediately after interaction with demonstrators indicating that the initial learning of this task was not impaired (MGI Ref ID J:62218)
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Neurobiology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Syt4tm1Hahe | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Harvey R Herschman | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Syt IV -/-; Syt4tm1Hrh; | ||
| Mutation Made By | Edwin Chapman, University of Wisconsin, Madison | ||
| Strain of Origin | 129X1/SvJ | ||
| Gene Symbol and Name | Syt4, synaptotagmin IV | ||
| Chromosome | 18 | ||
| Gene Common Name(s) | HsT1192; | ||
| Molecular Note | The gene was disrupted by insertion of a neo resistance cassette into an exon encoding the first C2 domain. Northern blot of total brain RNA from homozygous mutant animals verified the absence of gene expression. [MGI Ref ID J:62218] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Syt4tm1Hrh, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ferguson GD; Anagnostaras SG; Silva AJ; Herschman HR. 2000. Deficits in memory and motor performance in synaptotagmin IV mutant mice. Proc Natl Acad Sci U S A 97(10):5598-603. [PubMed: 10792055] [MGI Ref ID J:62218]
Additional References
Syt4tm1Hahe relatedArthur CP; Dean C; Pagratis M; Chapman ER; Stowell MH. 2010. Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons. Neuroscience 167(1):135-42. [PubMed: 20138128] [MGI Ref ID J:159708]
Dean C; Liu H; Dunning FM; Chang PY; Jackson MB; Chapman ER. 2009. Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release. Nat Neurosci 12(6):767-76. [PubMed: 19448629] [MGI Ref ID J:161262]
Ferguson GD; Herschman HR; Storm DR. 2004. Reduced anxiety and depression-like behavior in synaptotagmin IV (-/-) mice. Neuropharmacology 47(4):604-11. [PubMed: 15380377] [MGI Ref ID J:97193]
Johnson SL; Franz C; Kuhn S; Furness DN; Ruttiger L; Munkner S; Rivolta MN; Seward EP; Herschman HR; Engel J; Knipper M; Marcotti W. 2010. Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses. Nat Neurosci 13(1):45-52. [PubMed: 20010821] [MGI Ref ID J:156713]
Zhang G; Bai H; Zhang H; Dean C; Wu Q; Li J; Guariglia S; Meng Q; Cai D. 2011. Neuropeptide exocytosis involving synaptotagmin-4 and oxytocin in hypothalamic programming of body weight and energy balance. Neuron 69(3):523-35. [PubMed: 21315262] [MGI Ref ID J:174746]
Zhang Z; Bhalla A; Dean C; Chapman ER; Jackson MB. 2009. Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals. Nat Neurosci 12(2):163-71. [PubMed: 19136969] [MGI Ref ID J:146181]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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