Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male)   10-MAY-11 Species laboratory mouse Generation N2F2 (08-SEP-10) Generation Definitions

Description
The Tg(RP24-180N9)2Kjn transgene gives expression of Fscn2 and rescues the Fscn2^ahl8 related early onset hearing loss on the DBA/2J background. At one month of age the 16 kHz ABR threshold of the hemizygote is close to that of DBA/2NCrl controls, a subline that does not have Fscn2^ahl8. This transgenic rescue is similar to that found in the congenic rescue in D2.B6-Fscn2⁺/Kjn (stock #012438).

Development
The C57BL/6J-derived BAC RP24-180N9 was microinjected into the pronuclei of DBA/2J embryos. This transgene was backcrossed twice to DBA/2J then intercrossed to homozygosity.

Related Strains

Strains carrying other alleles of Fscn2

009629	B6.D2-Fscn2ahl8/4Kjn
012438	D2.B6-Fscn2+/Kjn
000671	DBA/2J

View Strains carrying other alleles of Fscn2     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Retinitis Pigmentosa 30; RP30   (FSCN2)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tg(RP24-180N9)2Kjn/0

        DBA/2J-Tg(RP24-180N9)2Kjn/Kjn

• hearing/vestibular/ear phenotype
• abnormal auditory brainstem response
  • at 1 month of age the 16kHz ABR threshold is close to that of DBA/2NCrl controls indicating rescue of the ahl8 associated early onset hearing loss   (MGI Ref ID J:162868)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tg(RP24-180N9)2Kjn related

Sensorineural Research
Hearing Defects
      Age related hearing loss, control

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(RP24-180N9)2Kjn
Allele Name		transgene insertion 2, Kenneth R Johnson
Allele Type		Transgenic (random, expressed)
Strain of Origin		DBA/2J
Expressed Gene		Fscn2, fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), mouse, laboratory
Promoter		Fscn2, fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), mouse, laboratory
Molecular Note		BAC clone RP24-180N9, derived from C57BL/6J and including the full Fscn2 gene and regulatory sequence, was microinjected into pronuclei of DBA/2J embryos and randomly integrated. This insertion gives detectable expression of Fscn2. [MGI Ref ID J:162868]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shin JB; Longo-Guess CM; Gagnon LH; Saylor KW; Dumont RA; Spinelli KJ; Pagana JM; Wilmarth PA; David LL; Gillespie PG; Johnson KR. 2010. The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice. J Neurosci 30(29):9683-94. [PubMed: 20660251]  [MGI Ref ID J:162868]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)   10-MAY-11

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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