Strain Name:

FVB/N-Tg(Myh6-Gnaq)40Gwd/J

Stock Number:

012460

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Availability:

Repository- Live

These Gαq-40 mice are hemizygous for the Gnaq allele with overexpression of Gαq in the heart. These mice may be useful for studying the biochemical and physiologic phenotype resembling both the compensated and decompensated phases of human cardiac hypertrophy.

Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   27-JUL-10
Specieslaboratory mouse
GenerationF?+N4F3 (12-DEC-13)
Generation Definitions
 
Donating Investigator Gerald Dorn,   Washington University School of Medicine

Description
Mice hemizygous for the Myh6-Gnaq allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator states that hemizygous females develop peripartum cardiomyopathy. Expression of Gαq is regulated by an α-myosin heavy chain (Myh6) promoter, which leads to overexpression of Gαq in the heart. This overexpression results in cardiac hypertrophy, defined as a conserved program of fetal gene expression, increased heart weight, and increased cardiomyocyte size, which severely compromises systolic cardiac function, and results in overt cardiac failure. Upon experimental pressure overloading, the mice progress rapidly to heart failure. These mice may be useful for studying the biochemical and physiologic phenotype resembling both the compensated and decompensated phases of human cardiac hypertrophy.

Development
A transgenic vector was generated including the entire coding region of the GTP-binding proteins class Gq protein subunit Gaq (Gnaq) gene under control of an α-myosin heavy chain (Myh6) promoter, followed by a simian virus 40 intron and a polyadenylation signal (polyA). The construct was microinjected into FVB/N fertilized oocytes, and founder mice were bred to FVB/N. Mice from founder line 40 (Gαq-40) were identified to establish a hemizygous colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myh6
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011038   B6.FVB-Tg(Myh6-cre)2182Mds/J
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002639   B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
002638   B6SJL-Tg(WTbeta2)4Wjk/J
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010587   FVB-Tg(Myh6-MEF2A)1Jmol/J
010581   FVB-Tg(Myh6-Map2k1*)1Jmol/J
010582   FVB-Tg(Myh6-Map2k3*)1Jmol/J
010584   FVB-Tg(Myh6-Map2k7)1Jmol/J
010585   FVB-Tg(Myh6-Mapk1)1Jmol/J
010583   FVB-Tg(Myh6-Mapk14*)1Jmol/J
010586   FVB-Tg(Myh6-Mef2c)2Jmol/J
009438   FVB-Tg(Myh6-SOD2,Tyr)3Pne/J
016570   FVB-Tg(Myh6-TRPC3*)6.6Jmol/J
017543   FVB-Tg(Myh6-TRPC6*)1Jmol/J
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010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
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012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
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012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
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012461   FVB/N-Tg(Myh6-Cast)1Gwd/J
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010579   STOCK Tg(Myh6-Prkca)1Jmk/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
005650   STOCK Tg(Myh6-cre/Esr1*)1Jmk/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
View Strains carrying other alleles of Myh6     (40 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Capillary Malformations, Congenital; CMC   (GNAQ)
Sturge-Weber Syndrome; SWS   (GNAQ)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Myh6-Gnaq)40Gwd/0

        involves: FVB/N
  • mortality/aging
  • premature death
    • most die by 5 weeks of age   (MGI Ref ID J:41949)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities
      cardiomyopathy

Developmental Biology Research
Internal/Organ Defects
      heart

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Myh6-Gnaq)40Gwd
Allele Name transgene insertion 40, Gerald W Dorn II
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) Galphaq-40; Tg(Myh6-Gnaq)2Gwd; Tg(Myhca-Gnaq)2Gwd;
Mutation Made By Gerald Dorn,   Washington University School of Medicine
Strain of OriginFVB/N
Expressed Gene Gnaq, guanine nucleotide binding protein, alpha q polypeptide, mouse, laboratory
Promoter Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine
Molecular Note A 1.46 kb mouse Gnaq cDNA sequence under regulatory control of a full length mouse Myhca promoter was used for the transgene. The mouse Myhca promoter was used to drive transgene expression in the heart. Transgenic line designated Galphaq-40 carries 40 copies of the transgene. [MGI Ref ID J:41949]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Myh6-Gnaq)MCA, High Resolution Melting
Tg(Myh6-Gnaq), Separated PCR
Tg(Myh6-Gnaq)MCA, Melt Curve Analysis


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tg(Myh6-Gnaq)40Gwd related

Biolo A; Greferath R; Siwik DA; Qin F; Valsky E; Fylaktakidou KC; Pothukanuri S; Duarte CD; Schwarz RP; Lehn JM; Nicolau C; Colucci WS. 2009. Enhanced exercise capacity in mice with severe heart failure treated with an allosteric effector of hemoglobin, myo-inositol trispyrophosphate. Proc Natl Acad Sci U S A 106(6):1926-9. [PubMed: 19204295]  [MGI Ref ID J:144938]

D'Angelo DD; Sakata Y; Lorenz JN; Boivin GP; Walsh RA; Liggett SB ; Dorn GW 2nd. 1997. Transgenic Galphaq overexpression induces cardiac contractile failure in mice. Proc Natl Acad Sci U S A 94(15):8121-6. [PubMed: 9223325]  [MGI Ref ID J:41949]

Dorn GW 2nd; Tepe NM; Lorenz JN; Koch WJ; Liggett SB. 1999. Low- and high-level transgenic expression of beta2-adrenergic receptors differentially affect cardiac hypertrophy and function in Galphaq- overexpressing mice. Proc Natl Acad Sci U S A 96(11):6400-5. [PubMed: 10339599]  [MGI Ref ID J:55199]

Lancel S; Qin F; Lennon SL; Zhang J; Tong X; Mazzini MJ; Kang YJ; Siwik DA; Cohen RA; Colucci WS. 2010. Oxidative posttranslational modifications mediate decreased SERCA activity and myocyte dysfunction in Galphaq-overexpressing mice. Circ Res 107(2):228-32. [PubMed: 20508180]  [MGI Ref ID J:175046]

Rogers JH; Tsirka A; Kovacs A; Blumer KJ; Dorn GW 2nd; Muslin AJ. 2001. RGS4 reduces contractile dysfunction and hypertrophic gene induction in Galpha q overexpressing mice. J Mol Cell Cardiol 33(2):209-18. [PubMed: 11162127]  [MGI Ref ID J:127759]

Roth DM; Bayat H; Drumm JD; Gao MH; Swaney JS; Ander A; Hammond HK. 2002. Adenylyl cyclase increases survival in cardiomyopathy. Circulation 105(16):1989-94. [PubMed: 11997288]  [MGI Ref ID J:133188]

Roth DM; Gao MH; Lai NC; Drumm J; Dalton N; Zhou JY; Zhu J; Entrikin D; Hammond HK. 1999. Cardiac-directed adenylyl cyclase expression improves heart function in murine cardiomyopathy. Circulation 99(24):3099-102. [PubMed: 10377071]  [MGI Ref ID J:133194]

Satoh M; Matter CM; Ogita H; Takeshita K; Wang CY; Dorn GW 2nd; Liao JK. 2007. Inhibition of apoptosis-regulated signaling kinase-1 and prevention of congestive heart failure by estrogen. Circulation 115(25):3197-204. [PubMed: 17562954]  [MGI Ref ID J:137560]

Song Q; Schmidt AG; Hahn HS; Carr AN; Frank B; Pater L; Gerst M; Young K; Hoit BD; McConnell BK; Haghighi K; Seidman CE; Seidman JG; Dorn GW 2nd; Kranias EG. 2003. Rescue of cardiomyocyte dysfunction by phospholamban ablation does not prevent ventricular failure in genetic hypertrophy. J Clin Invest 111(6):859-67. [PubMed: 12639992]  [MGI Ref ID J:82521]

Takimoto E; Koitabashi N; Hsu S; Ketner EA; Zhang M; Nagayama T; Bedja D; Gabrielson KL; Blanton R; Siderovski DP; Mendelsohn ME; Kass DA. 2009. Regulator of G protein signaling 2 mediates cardiac compensation to pressure overload and antihypertrophic effects of PDE5 inhibition in mice. J Clin Invest 119(2):408-20. [PubMed: 19127022]  [MGI Ref ID J:146192]

Tepe NM; Liggett SB. 1999. Transgenic replacement of type V adenylyl cyclase identifies a critical mechanism of beta-adrenergic receptor dysfunction in the G alpha q overexpressing mouse. FEBS Lett 458(2):236-40. [PubMed: 10481072]  [MGI Ref ID J:57726]

Timofeyev V; He Y; Tuteja D; Zhang Q; Roth DM; Hammond HK; Chiamvimonvat N. 2006. Cardiac-directed expression of adenylyl cyclase reverses electrical remodeling in cardiomyopathy. J Mol Cell Cardiol 41(1):170-81. [PubMed: 16750219]  [MGI Ref ID J:110971]

Timofeyev V; Porter CA; Tuteja D; Qiu H; Li N; Tang T; Singapuri A; Han PL; Lopez JE; Hammond HK; Chiamvimonvat N. 2010. Disruption of adenylyl cyclase type V does not rescue the phenotype of cardiac-specific overexpression of Galphaq protein-induced cardiomyopathy. Am J Physiol Heart Circ Physiol 299(5):H1459-67. [PubMed: 20709863]  [MGI Ref ID J:166641]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, transgenic mice may be bred to wildtype (noncarrier) mice from the colony or with FVB/NJ inbred mice (Stock No. 001800). Transgenic females develop peripartum cardiomyopathy.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   27-JUL-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(Myh6-Gnaq)40Gwd  
Price per Pair (US dollars $)Pair Genotype
$311.00Hemizygous for Tg(Myh6-Gnaq)40Gwd x Noncarrier  
$311.00Noncarrier x Hemizygous for Tg(Myh6-Gnaq)40Gwd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(Myh6-Gnaq)40Gwd  
Price per Pair (US dollars $)Pair Genotype
$404.30Hemizygous for Tg(Myh6-Gnaq)40Gwd x Noncarrier  
$404.30Noncarrier x Hemizygous for Tg(Myh6-Gnaq)40Gwd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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