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| These FLAG-Lrrk2-WT mice express a wild-type mouse leucine-rich repeat kinase 2 (Lrrk2) gene directed by its endogenous promoter/enhancer regions on a BAC transgene. These mice may be useful for studying normal function of LRRK2 function and the pathogenesis of Parkinson's disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N12pN1
Generation DefinitionsDonating Investigator Zhenyu Yue, Mount Sinai School of Medicine Description
Mice hemizygous for the BAC FLAG-Lrrk2-Wt transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a wild-type mouse leucine-rich repeat kinase 2 (Lrrk2) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. These BAC FLAG-Lrrk2-Wt mice "overexpress" the wildtype mouse LRRK2 protein in cerebral cortex, striatum, substantia nigra, internal capsule, and hippocampus at an approximately 6-8 fold greater level than endogenous mouse Lrrk2. Contrary to the BAC FLAG-Lrrk2-G2019S Parkinson's disease mice, these mice have enhanced evoked dopamine release in the striatum and exhibit slightly increased motor activities compared to nontransgenic wild-type controls. These mice may be useful for studying normal function of LRRK2 function and the pathogenesis of Parkinson's disease.Development
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 240 kb BAC (#RP23-312I9) containing the entire mouse Lrrk2 (leucine-rich repeat kinase 2) gene. This BAC, containing a FLAG-tag downstream from the start codon, was microinjected into fertilized B6C3 F1 oocytes. FLAG-Lrrk2-WT founder line 6 was established and then subsequently maintained by breeding transgenic mice with C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Parkinson's Disease Models
View Parkinson's Disease Models (109 strains)
012453 B6.129X1(FVB)-Lrrk2tm1.1Cai/J 009346 B6.Cg-Lrrk2tm1.1Shn/J 012467 B6.Cg-Tg(Lrrk2*G2019S)2Yue/J 009347 B6;129-Lrrk2tm1.1Shn/J 016209 B6;129-Lrrk2tm2.1Shn/J 016210 B6;129-Lrrk2tm3.1Shn/J 012444 C57BL/6-Lrrk2tm1Mjfa/J 016122 C57BL/6N-Lrrk1tm1.1Mjff Lrrk2tm1.1Mjff/J 016121 C57BL/6N-Lrrk2tm1.1Mjff/J
Additional Web Information
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Parkinson Disease 8, Autosomal Dominant; PARK8 (LRRK2)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Lrrk2)6Yue/0
involves: C3H * C57BL/6 * C57BL/6J
- nervous system phenotype
- *normal* nervous system phenotype
- mice exhibit normal neuronal survival (MGI Ref ID J:157931)
- increased neurotransmitter release
- tau protein deposits
- mice exhibit fewer tau deposits than wild-type mice or Tg(Lrrk2*G2019S)2Yue mice (MGI Ref ID J:157931)
- behavior/neurological phenotype
- enhanced coordination
- compared with wild-type mice and Tg(Lrrk2*G2019S)2Yue mice (MGI Ref ID J:157931)
- hyperactivity
- in an open field test, mice exhibit an increased number of rearings, movement, and distance traveled compared with wild-type mice (MGI Ref ID J:157931)
- increased vertical activity
- in an open field test (MGI Ref ID J:157931)
- long stride length
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
brain
Neurobiology Research
Neurodevelopmental Defects
Parkinson's Disease
LRRK2 strains
cell markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Lrrk2)6Yue | ||
|---|---|---|---|
| Allele Name | transgene insertion 6, Zhenyu Yue | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | FLAG-LRRK2-WT; | ||
| Mutation Made By | Zhenyu Yue, Mount Sinai School of Medicine | ||
| Strain of Origin | (C57BL/6 x C3H)F1 | ||
| Expressed Gene | Lrrk2, leucine-rich repeat kinase 2, mouse, laboratory | ||
| Promoter | Lrrk2, leucine-rich repeat kinase 2, mouse, laboratory | ||
| Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 240 kb BAC (#RP23-312I9) containing the entire mouse Lrrk2 (leucine-rich repeat kinase 2) gene. This BAC contains a FLAG-tag downstream from the start codon. Founder line 6 was established with expression in cerebral cortex, striatum, substantia nigra, internal capsule, and hippocampus at an approximately 6-8 fold greater level than endogenous mouse Lrrk2. [MGI Ref ID J:128036] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Lrrk2), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Li X; Tan YC; Poulose S; Olanow CW; Huang XY; Yue Z. 2007. Leucine-rich repeat kinase 2 (LRRK2)/PARK8 possesses GTPase activity that is altered in familial Parkinson's disease R1441C/G mutants. J Neurochem 103(1):238-47. [PubMed: 17623048] [MGI Ref ID J:128036]
Additional References
Tg(Lrrk2)6Yue relatedLi X; Patel JC; Wang J; Avshalumov MV; Nicholson C; Buxbaum JD; Elder GA; Rice ME; Yue Z. 2010. Enhanced striatal dopamine transmission and motor performance with LRRK2 overexpression in mice is eliminated by familial Parkinson's disease mutation G2019S. J Neurosci 30(5):1788-97. [PubMed: 20130188] [MGI Ref ID J:157931]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J (Stock No. 000664).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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