Description

Cell Line Information

Name	FVB/NJ-PB84.3 mES cells

Description
The FVB/NJ-PB84.3 mES cells were provided by Ted Choi, PhD of of Predictive Biology, Inc. FVB/NJ (Stock No. 001800) mice are an albino strain commonly used for transgenic strain creation. This strain is homozygous for retinal degeneration allele Pde6b^rd1. The FVB/NJ-PB84.3 mES cells are being provided at either passage 9 and 10.

mES cells are cultured in mES cell medium as described in the product spec sheet and replenished daily. mES cells are maintained on irradiated primary mouse embryonic fibroblasts (MEFs) from C57BL/6J or B6D2F1/J mice. Therefore, each vial of mES cells contain a small number of irradiated fibroblast feeder cells.

Additional Web Information

Product Spec Sheet for FVB/NJ-PB84.3 mES cells

Research Applications

Research Application Information

Genes & Alleles

Gene & Allele Information provided by MGI

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

Quality assurance

Quality Assurance Information

JAX^® mES cells are tested for bacterial and fungal growth and for the presence of mycoplasma using a PCR detection system. Cell culture contaminants were not detected. The genotype of the cell line was confirmed by SNP analysis. mES cells will spontaneously differentiate in the absence of mouse embryonic fibroblasts (MEFs) and supplemental growth factors, which together act as inhibitors of differentiation. Germ line competency testing for FVB/NJ-PB84.3 mES cells is currently underway. JAX makes no guarantee that this cell line can be used to generate targeted clones or, if targeted clones are created, that they will be germ line competent.

References

References provided by MGI

Currently there are no available references for this strain.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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General Terms and Conditions

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General inquiries regarding Terms of Use

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JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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