Strain Name:

B6.129S7(129S4)-Ift20tm1.1Gjp/J

Stock Number:

012565

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Mice homozygous for this Ift20flox allele are viable and fertile, with loxP sites flanking exons 2-3 of the Ift20 locus. When bred to mice that express Cre recombinase, the resulting offspring will have sequences encoding the first 71 codons (including the start codon) deleted in the cre-expressing tissues; this is expected to produce a null allele.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN10pN1
Generation Definitions
 
Donating Investigator Gregory J Pazour,   University of Massachusetts Medical Sch.

Description
These Ift20flox mice harbor loxP sites flanking exons 2-3 (encoding the first 71 codons including the start codon) of the intraflagellar transport 20 homolog (Chlamydomonas) locus. The primary cilium is a microtubule-based antenna-like structure that emanates from the surface of virtually all cells in the mammalian body. The primary cilium functions as a sensory organelle (mechano-, chemo-, photo-receptor) that receives signals from other cells/the environment, and transmits these signals to the nucleus to elicit a cellular response. Most types of eukaryotic cilia and flagella are assembled and maintained by the process of intraflagellar transport (IFT). During IFT, large protein complexes (IFT particles) are transported along the ciliary microtubules under the ciliary membrane. IFT particle proteins organize into at least three distinct complexes called complex A, complex B and the Golgi IFT complex. The unique role of Ift20 in both complex B as well as the Golgi IFT complex is particularly noteworthy. Because of the widespread presence of primary cilia in multiple organ systems, these Ift20flox mutant mice may be useful in generating conditional mutations for studying IFT proteins in eukaryotic ciliary assembly, primary cilium in cell signaling pathways associated with embryonic development and tissue homeostasis in adults, as well as carcinogenesis/cancer, cystic kidney disease, retinal degeneration/blindness, obesity, and other diseases.

For example, when Ift20flox mice are bred with mice expressing Cre recombinase in the male germline (Prm-Cre: see Stock No. 003328), the resulting offspring have pan-deletion of Ift20 function; resulting in embryonic lethality. In addition, when Ift20flox mice are bred with mice expressing Cre recombinase in during embryonic kidney development (HoxB7-Cre: see Stock No. 004692), the resulting offspring have deletion of Ift20 function in the mechanoreceptors of the kidney tubule epithelium/collecting ducts; resulting in polycystic kidney disease.

Development
A targeting vector was designed to insert a frt-flanked neomycin cassette and loxP site upstream of exon 2, and a second loxP site (together with an NdeI site) downstream of exon 3 of the Ift20 (intraflagellar transport 20 homolog (Chlamydomonas)) locus. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2 derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6J mice to establish the Ift20neo colony. The Ift20neo mice were then bred to FLPeR mice (on a C57BL/6J;129S4 genetic background; see Stock No. 003946) to remove the frt-flanked neo cassette. The resulting Ift20flox mice (with a single frt site and loxP site remaining upstream of exon 2, and a second loxP site downstream of exon 3) were selected. The dontating investigator reported that these Ift20flox mice were backcrossed to C57BL/6J (see SNP note below) for ten generations (and the FLPe-expressing mutation was removed) prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Ift20tm1.1Gjp/Ift20tm1.1Gjp Tg(Hoxb7-cre)13Amc/0

        involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6   (conditional)
  • renal/urinary system phenotype
  • abnormal kidney morphology
    • by P23 most of the kidney is replaced by collecting duct epithelium, fluid filled cysts, and fibrotic material   (MGI Ref ID J:141071)
    • abnormal kidney collecting duct epithelium morphology
      • at E18 collecting duct epithelial cells almost completely lack cilia   (MGI Ref ID J:141071)
      • at P5 in non-dilated tubules, mitotic spindle orientation is randomized rather than being parallel to the long axis of the tubule   (MGI Ref ID J:141071)
      • at P5 the centrosome remains at the apical end of the cell but can be located anywhere from the lateral junction to the center of the cell rather than at the center of the cell   (MGI Ref ID J:141071)
    • abnormal kidney epithelial cell primary cilium morphology
      • at E18 collecting duct epithelial cells almost completely lack cilia   (MGI Ref ID J:141071)
    • dilated kidney collecting duct
      • dilation is first seen at P5   (MGI Ref ID J:141071)
    • enlarged kidney
      • progressive bilateral kidney enlargement beginning by P10   (MGI Ref ID J:141071)
      • increased kidney weight
        • by P23, kidney weight is about 10 times that of controls   (MGI Ref ID J:141071)
    • kidney cysts
      • cystic expansion of the collecting ducts   (MGI Ref ID J:141071)
      • cells lining cysts lack primary cilia   (MGI Ref ID J:141071)
      • cells lining cysts may be flat with prominent microvilli or domed with less pronounced microvilli   (MGI Ref ID J:141071)
    • renal interstitial fibrosis   (MGI Ref ID J:141071)
  • increased kidney cell proliferation
    • by P23, collecting duct cells lacking cilia show increased rates of proliferation   (MGI Ref ID J:141071)
  • homeostasis/metabolism phenotype
  • increased blood urea nitrogen level
    • about 3 times higher than in controls   (MGI Ref ID J:141071)
  • cellular phenotype
  • increased kidney cell proliferation
    • by P23, collecting duct cells lacking cilia show increased rates of proliferation   (MGI Ref ID J:141071)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cancer Research
Cardiovascular Research
      Cre-lox System
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
Internal/Organ Research
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ift20tm1.1Gjp
Allele Name targeted mutation 1.1, Gregory J Pazour
Allele Type Targeted (Floxed/Frt)
Common Name(s) ift20flox;
Mutation Made By Gregory Pazour,   University of Massachusetts Medical Sch.
Strain of Origin129S7/SvEvBrd-Hprt
Gene Symbol and Name Ift20, intraflagellar transport 20
Chromosome 11
Gene Common Name(s) 0610009H04Rik; AU015496; RGD1309400; RIKEN cDNA 0610009H04 gene; expressed sequence AU015496;
Molecular Note An frt flanked neo cassette followed by a loxP site were inserted into intron 1 and a second loxP site was inserted into intron 3 via homologous recombination. The neo cassette was then removed via in vivo Flp mediated recombination. [MGI Ref ID J:141071]

Genotyping

Genotyping Information

Genotyping Protocols

Ift20tm1.1Gjp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jonassen JA; San Agustin J; Follit JA; Pazour GJ. 2008. Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease. J Cell Biol 183(3):377-84. [PubMed: 18981227]  [MGI Ref ID J:141071]

Additional References

Ift20tm1.1Gjp related

Amador-Arjona A; Elliott J; Miller A; Ginbey A; Pazour GJ; Enikolopov G; Roberts AJ; Terskikh AV. 2011. Primary cilia regulate proliferation of amplifying progenitors in adult hippocampus: implications for learning and memory. J Neurosci 31(27):9933-44. [PubMed: 21734285]  [MGI Ref ID J:174559]

Keady BT; Le YZ; Pazour GJ. 2011. IFT20 is required for opsin trafficking and photoreceptor outer segment development. Mol Biol Cell 22(7):921-30. [PubMed: 21307337]  [MGI Ref ID J:183002]

Ma M; Tian X; Igarashi P; Pazour GJ; Somlo S. 2013. Loss of cilia suppresses cyst growth in genetic models of autosomal dominant polycystic kidney disease. Nat Genet 45(9):1004-12. [PubMed: 23892607]  [MGI Ref ID J:205308]

McDermott KM; Liu BY; Tlsty TD; Pazour GJ. 2010. Primary Cilia Regulate Branching Morphogenesis during Mammary Gland Development. Curr Biol :. [PubMed: 20381354]  [MGI Ref ID J:161973]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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