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| Mouse strains created by the Pleiades Promoter Project (Dr. Elizabeth M. Simpson, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Canada) are now available through the Mutant Mouse Regional Resource Center (MMRRC) at The Jackson Laboratory. For the Ple134-lacZ mice, please see MMRRC #032958 at the MMRRC website. | |||||||||||||||||||
| These mice have the Ple134-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human NOV gene direct expression of β-galactosidase. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
Strain Information
Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N3pN1
Generation DefinitionsDonating Investigator Elizabeth M Simpson, University of British Columbia
Related Strains
Pleiades Promoter Project lacZ strains
View Pleiades Promoter Project lacZ strains (20 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Hprttm69(Ple134-lacZ)Ems/Y
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J
- normal phenotype
- no abnormal phenotype detected
- heterozygous females and hemizygous males are viable and fertile (MGI Ref ID J:145689)
This mouse can be used to support research in many areas including:
Neurobiology Research
lacZ expression in neural tissue
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Hprttm69(Ple134-lacZ)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 69, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprttm69(mEMS4521)Ems; NOV-lacZ; Ple134; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | (B6.129P2-Hprt | ||
| Site of Expression | lacZ is expressed in areas of the brain. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | NOV, nephroblastoma overexpressed, human | ||
| General Note | Germ line transmission of mutant cell line mEMS4521 has been established. | ||
| Molecular Note | The Ple134-lacZ transgene (bEMS91) was designed with the Ple134 MaxiPromoter (NOV-X; derived from the BAC RP11-840I14 that contains the whole nephroblastoma overexpressed (NOV) gene (along with ~140 kb of 5' and ~40 kb of 3' flanking sequences)) upstreamof a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. [MGI Ref ID J:144244] [MGI Ref ID J:164356] | ||
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; Hgprtase; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Hprttm69(Ple134-lacZ)Ems, Standard PCR
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Gt(ROSA)26Sortm1sor STD, Standard PCR
Tyrc-2J, End Point Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Hprttm69(Ple134-lacZ)Ems relatedPortales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF; Bonaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Simpson EM:. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 107(38):16589-94. [PubMed: 20807748] [MGI Ref ID J:164356]
The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699] [MGI Ref ID J:144244]
Health & husbandry
Health & Colony Maintenance Information
Currently there is no information available for this strain. This may be due to the supply level of this strain.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
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