|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about B6.129S6-Ptpn11tm1Toa/Mmjax MMRRC Stock Number 032103.|
|These mutant mice possess a loxP-STOP-loxP cassette between exons 2 and 3 and a D61Y point mutation in exon 3 of the protein tyrosine phosphatase, non-receptor type 11 (Ptpn11) gene and may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).|
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1
Donating Investigator Benjamin Neel, Ontario Cancer Institute
Mice that are homozygous for the targeted mutation are not viable. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).
When bred to a strain expressing Cre recombinase in endothelial cells (Tg(Tek-cre)12Flv), this mutant mouse strain may be useful in studies of the cardiac defects found in Noonan syndrome.
When bred to a strain expressing Cre recombinase in epiblast-derived tissues (Meox2tm1(cre)Sor), this mutant mouse strain may be useful in studies of cardiac defects.
When bred to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (Tg(CAG-cre/Esr1*)5Amc), this mutant mouse strain may be useful in studies of hematopoiesis.
When bred to a strain expressing interferon-inducible Cre recombinase in most tissues (Tg(Mx1-cre)1Cgn), this mutant mouse strain may be useful in studies of fatal myeloproliferative disorder and juvenile myelomonocytic leukemia (JMML).
When bred to a strain expressing Cre recombinase in neural tube, spinal cord and areas of the brain (Tg(Wnt1-cre)11Rth), this mutant mouse strain may be useful in studies of craniofacial defects.
When bred to a strain expressing Cre recombinase in the early mouse embryo (Tg(EIIa-cre)C5379Lmgd), this mutant mouse strain may be useful in studies of myeloproliferative disorders.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to place a loxP-neo-loxP-STOP-loxP cassette between exons 2 and 3 and to insert a D61Y point mutation and unique AgeI restriction enzyme site in exon 3 of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for a minimum of 10 generations. Upon arrival, mice were bred to C57LB/6J for at least 1 generation to establish the colony.
|Considerations for Choosing Controls|
Strains carrying other alleles of Ptpn11
012591 B6.129S4-Ptpn11tm1Yan/Mmjax 012593 B6;129S4-Ptpn11tm1Bgn/Mmjax 025758 FVB.129P2(Cg)-Ptpn11tm1.1Wbm/JView Strains carrying other alleles of Ptpn11 (3 strains)
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGIView Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
|Allele Name||targeted mutation 1, Toshiyuki Araki|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Common Name(s)||Ptpn11inD61Y; inDY;|
|Mutation Made By||Benjamin Neel, Ontario Cancer Institute|
|Strain of Origin||129S6/SvEvTac|
|Gene Symbol and Name||Ptpn11, protein tyrosine phosphatase, non-receptor type 11|
|Gene Common Name(s)||2700084A17Rik; AW536184; BPTP3; CFC; NS1; PTP-1D; PTP1D; PTP2C; RIKEN cDNA 2700084A17 gene; SH-PTP2; SH-PTP3; SH2 domain-containing protein tyrosine phosphatase-2; SHP-2; SHP2; Syp; expressed sequence AW536184;|
|Molecular Note||A cassette consisting of a loxP site, neo, loxP site, stop signal, and a third loxP site was inserted into intron 2 and a D61Y mutation was inserted into exon 3 via homologous recombination. [MGI Ref ID J:147154] [MGI Ref ID J:148430]|
Araki T; Chan G; Newbigging S; Morikawa L; Bronson RT; Neel BG. 2009. Noonan syndrome cardiac defects are caused by PTPN11 acting in endocardium to enhance endocardial-mesenchymal transformation. Proc Natl Acad Sci U S A 106(12):4736-41. [PubMed: 19251646] [MGI Ref ID J:147154]
Chan G; Kalaitzidis D; Usenko T; Kutok JL; Yang W; Mohi MG; Neel BG. 2009. Leukemogenic Ptpn11 causes fatal myeloproliferative disorder via cell-autonomous effects on multiple stages of hematopoiesis. Blood 113(18):4414-24. [PubMed: 19179468] [MGI Ref ID J:148430]
Goodwin CB; Li XJ; Mali RS; Chan G; Kang M; Liu Z; Vanhaesebroeck B; Neel BG; Loh ML; Lannutti BJ; Kapur R; Chan RJ. 2014. PI3K p110delta uniquely promotes gain-of-function Shp2-induced GM-CSF hypersensitivity in a model of JMML. Blood 123(18):2838-42. [PubMed: 24553178] [MGI Ref ID J:210890]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
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