Strain Name:

B6;129S4-Mapttm3(HDAC2)Jae/J

Stock Number:

012639

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
In mice homozygous for the HDAC2OE allele, overexpression of Hdac2 is regulated by microtubule-associated protein tau Mapt gene, which leads to neuron-specific overexpression of Hdac2. This strain may be useful for studying the functional and structural synaptic changes and neuronal adaptive responses implicated in memory formation and storage.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Li-Huei Tsai,   Massachusetts Institue of Technology

Description
Mice homozygous for the HDAC2OE allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of Hdac2 from the microtubule-associated protein tau (Mapt) locus, results in neuron-specific overexpression of Hdac2. This overexpression results in a 2-3 fold expression of Hdac2, increased histone deacetylation, and impaired synaptic plasticity in the hippocampus leading to a negative function in regulating memory formation. This strain may be useful for studying the functional and structural synaptic changes and neuronal adaptive responses implicated in memory formation and storage.

Development
A targeting vector was designed to insert mouse histone deacetylase 2 (Hdac2) cDNA into exon 1 of the microtubule-associated protein tau (Mapt) gene, followed by a polyadenylation (polyA) signal, and a floxed PGK neomycin (neo) resistance cassette. The construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid for the purpose of removing the neo cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette were injected into (DBA/2 x C57BL/6)F1 blastocysts. The resulting chimeric males were bred to C57BL/6 females, and their offspring were crossed to generated a colony of HDAC2OE mice. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

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View Alzheimer's Disease Models     (109 strains)

Strains carrying other alleles of Hdac2
022625   B6.Cg-Hdac2tm1.1Rdp/J
012640   STOCK Hdac2tm1.2Rdp/J
View Strains carrying other alleles of Hdac2     (2 strains)

View Strains carrying other alleles of Mapt     (13 strains)

Additional Web Information

Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Frontotemporal Dementia; FTD   (MAPT)
Parkinson Disease, Late-Onset; PD   (MAPT)
Parkinson-Dementia Syndrome   (MAPT)
Pick Disease of Brain   (MAPT)
Supranuclear Palsy, Progressive, 1; PSNP1   (MAPT)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mapttm3(Hdac2)Jae/Mapttm3(Hdac2)Jae

        involves: 129S4/SvJae * C57BL/6
  • behavior/neurological phenotype
  • abnormal spatial learning
    • mice have increased escape latency throughout a 9 day training process in a Morris water maze   (MGI Ref ID J:148391)
    • mice do not show a preference for the target quadrant when the hidden platform is removed whereas the control mice spend significantly more time in the target quadrant   (MGI Ref ID J:148391)
  • abnormal spatial working memory
    • mice show impairment of spatial working memory in a T-maze non-matching-to-place task with a third less correct guesses than controls after 2 days of training   (MGI Ref ID J:148391)
  • impaired contextual conditioning behavior
    • show markedly decreased freezing behaviour in both context-dependent and tone-dependent fear learning   (MGI Ref ID J:148391)
    • time spent in a freezing position is about half that of controls for the context-depending test and only about 1/10th of controls for tone-dependent testing   (MGI Ref ID J:148391)
  • nervous system phenotype
  • abnormal dentate gyrus morphology
    • the density of dendritic spines along dentate gyrus granule cells is markedly decreased   (MGI Ref ID J:148391)
  • abnormal hippocampus CA1 region morphology
    • the number of presynaptic terminals of functional synapses is significantly decreased in the CA1 striatum radiatum   (MGI Ref ID J:148391)
  • abnormal hippocampus pyramidal cell morphology
    • the overall level of acetylated lysine on histones is decreased in these neurons compared to controls   (MGI Ref ID J:148391)
    • the density of dendritic spines along individual dendrites of hippocampal CA1 pyramidal neurons is markedly decreased   (MGI Ref ID J:148391)
  • reduced long term potentiation
    • LTP induced by two trains of high frequency stimulation in CA1 neurons is impaired   (MGI Ref ID J:148391)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
      Tau (Mapt) mutants
Neurodegeneration

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mapttm3(Hdac2)Jae
Allele Name targeted mutation 3, Rudolf Jaenisch
Allele Type Targeted
Common Name(s) HDAC2OE; TauHDAC2;
Mutation Made ByDr. Rudolf Jaenisch,   Whitehead Institute (MIT)
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Expressed Gene Hdac2, histone deacetylase 2, mouse, laboratory
Gene Symbol and Name Mapt, microtubule-associated protein tau
Chromosome 11
Gene Common Name(s) AI413597; AW045860; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; Mtapt; PPND; PPP1R103; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau;
Molecular Note Coding sequence for the mouse Hdac2 protein was placed in-frame with exon 1 of the gene locus creating a fusion protein containing the first 31 amino-acids of the endogenous locus. A 2-3 fol expression of Hdac2 was confirmed in the CA1 region of the hippocampus by immunohistofluorescence. Hdac2 activity was confirmed by decreased levels of acetylation present in the same region as expression. [MGI Ref ID J:148391]
 

Genotyping

Genotyping Information

Genotyping Protocols

Mapttm3(HDAC2)Jae alternate 1,

Separated MCA


Mapttm3(HDAC2)Jaealternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Guan JS; Haggarty SJ; Giacometti E; Dannenberg JH; Joseph N; Gao J; Nieland TJ; Zhou Y; Wang X; Mazitschek R; Bradner JE; DePinho RA; Jaenisch R; Tsai LH. 2009. HDAC2 negatively regulates memory formation and synaptic plasticity. Nature 459(7243):55-60. [PubMed: 19424149]  [MGI Ref ID J:148391]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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