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| Ly6a-GFP transgenic mice have an enhanced green fluorescent protein (EGFP) under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. These mice may be useful for the visualizing and sorting of hematopoietic stem cells. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
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- Health & husbandry
- References
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Description
Strain Information
Former Names B6.Cg-Tg(Ly6a-GFP)G5Dzk/J (Changed: 26-MAR-10 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Noncarrier (Female x Male) 27-JUL-10 Species laboratory mouse Generation N11+F2 (30-JUN-11)
Generation DefinitionsDonating Investigator Elaine Dzierzak, Erasmus MC Description
Ly6a-GFP transgenic mice have an enhanced green fluorescent protein (EGFP) under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. Hemizygous Ly6a-GFP mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Ly6a promoter directs the expression of GFP in all functional repopulating adult hematopoietic stem cells (HSCs) in the adult bone marrow, and several other hematopoietic cell types. The GFP transgene expression pattern generally corresponds to that of Sca-1, a glycoprotein-I-linked cell-surface glycoprotein used routinely as a marker of adult hematopoietic stem cells. These mice may be useful for the visualizing and sorting of hematopoietic stem cells.Development
The Ly6a-GFP transgene contains an enhanced green fluorescent protein (EGFP) and the simian virus 40 (SV40) polyadenylation (polyA) sequence under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. The construct was microinjected into fertilized (C57BL/10 x CBA)F1 oocytes, and offspring from founder line G5 were bred to (C57BL/10 x CBA)F1 mice to establish a colony. The donating investigator reported that these mice were then backcrossed to C57BL/6J (see SNP note below) for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Ly6a-EGFP)G5Dzk/0
involves: C57BL/10 * CBA
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:158054)
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Genetics Research
Tissue/Cell Markers
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ly6a-EGFP)G5Dzk | ||
|---|---|---|---|
| Allele Name | transgene insertion G5, Elaine Dzierzak | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Ly6a-GFP; | ||
| Strain of Origin | (C57BL/10 x CBA)F1 | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Ly6a, lymphocyte antigen 6 complex, locus A, mouse, laboratory | ||
| Molecular Note | The Ly6a-GFP transgene contains an enhanced green fluorescent protein (EGFP) and the simian virus 40 (SV40) polyadenylation (polyA) sequence under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. Several lines were created. Line G5 has 8 copies of the transgene. [MGI Ref ID J:158054] | ||
| Gene Symbol and Name | Tg(Ly6a-EGFP)G5Dzk, transgene insertion G5, Elaine Dzierzak | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Ly6a-GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ma X; Robin C; Ottersbach K; Dzierzak E. 2002. The Ly-6A (Sca-1) GFP transgene is expressed in all adult mouse hematopoietic stem cells. Stem Cells 20(6):514-21. [PubMed: 12456959] [MGI Ref ID J:158054]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony hemizygous transgenic mice may be bred to wildtype (noncarrier) mice from the colony, or C57BL/6J (Stock No. 000664). Mating System Hemizygote x Noncarrier (Female x Male) 27-JUL-10 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Ly6a-EGFP)G5Dzk
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Ly6a-EGFP)G5Dzk x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Ly6a-EGFP)G5Dzk Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Ly6a-EGFP)G5Dzk
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Ly6a-EGFP)G5Dzk x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Ly6a-EGFP)G5Dzk Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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